Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3311

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
3,824,656	-		Pf6N2E2_3310	0.43	-0.1
3,824,656	-		Pf6N2E2_3310	0.43	+1.4
3,824,760	+		Pf6N2E2_3310	0.49	+1.4
3,824,841	+		Pf6N2E2_3310	0.54	-0.0
3,824,856	-		Pf6N2E2_3310	0.55	+1.3
3,824,856	-		Pf6N2E2_3310	0.55	+0.1
3,824,856	-		Pf6N2E2_3310	0.55	-0.8
3,824,868	+		Pf6N2E2_3310	0.56	-3.0
3,824,868	+		Pf6N2E2_3310	0.56	+1.2
3,824,868	+		Pf6N2E2_3310	0.56	-0.2
3,824,869	-		Pf6N2E2_3310	0.56	-0.5
3,824,869	-		Pf6N2E2_3310	0.56	-0.2
3,824,869	-		Pf6N2E2_3310	0.56	-0.8
3,824,869	-		Pf6N2E2_3310	0.56	+0.2
3,824,869	-		Pf6N2E2_3310	0.56	-1.5
3,824,869	-		Pf6N2E2_3310	0.56	+0.5
3,824,869	-		Pf6N2E2_3310	0.56	+0.6
3,824,869	-		Pf6N2E2_3310	0.56	+0.4
3,824,925	+		Pf6N2E2_3310	0.59	+0.4
3,825,302	+		Pf6N2E2_3310	0.83	-0.9
3,825,303	-		Pf6N2E2_3310	0.83	-0.4
3,825,303	-		Pf6N2E2_3310	0.83	+0.4
3,825,340	-		Pf6N2E2_3310	0.85	-1.0
3,825,340	-		Pf6N2E2_3310	0.85	+1.4
3,825,340	-		Pf6N2E2_3310	0.85	+0.1
3,825,423	+				+0.9
3,825,423	+				-0.9
3,825,424	-				-0.6
3,825,424	-				-1.2
3,825,511	+				-0.1
3,825,512	-				+1.4
3,825,519	+				-0.9
3,825,519	+				+0.5
3,825,519	+				-1.3
3,825,519	+				+1.4
3,825,519	+				-0.0
3,825,519	+				-0.3
3,825,520	-				-0.9
3,825,520	-				+0.2
3,825,570	+				-0.6
3,825,570	+				-0.8
3,825,570	+				+0.5
3,825,570	+				-0.2
3,825,571	-				+0.3
3,825,571	-				+1.4
3,825,571	-				-0.9
3,825,571	-				-0.9
3,825,574	+				+0.4
3,825,574	+				-0.5
3,825,575	-				-0.9
3,825,575	-				+2.4
3,825,635	+				-0.6
3,825,635	+				+0.1
3,825,636	-				-1.2
3,825,636	-				-1.7
3,825,636	-				-0.0
3,825,636	-				-1.0
3,825,636	-				+0.6
3,825,657	+				-0.0
3,825,657	+				+0.2
3,825,822	+		Pf6N2E2_3311	0.17	-1.4
3,825,832	+		Pf6N2E2_3311	0.18	-2.0
3,825,832	+		Pf6N2E2_3311	0.18	+0.4
3,825,833	-		Pf6N2E2_3311	0.18	-0.3
3,825,842	-		Pf6N2E2_3311	0.19	-0.7
3,825,851	-		Pf6N2E2_3311	0.20	-1.4
3,826,163	-		Pf6N2E2_3311	0.51	+0.1
3,826,342	+		Pf6N2E2_3311	0.69	-0.2
3,826,342	+		Pf6N2E2_3311	0.69	-0.6
3,826,342	+		Pf6N2E2_3311	0.69	-1.2
3,826,343	-		Pf6N2E2_3311	0.69	-0.9
3,826,343	-		Pf6N2E2_3311	0.69	-2.0
3,826,343	-		Pf6N2E2_3311	0.69	-0.4
3,826,343	-		Pf6N2E2_3311	0.69	-0.9
3,826,343	-		Pf6N2E2_3311	0.69	-1.1
3,826,458	-		Pf6N2E2_3311	0.80	-0.8
3,826,544	-		Pf6N2E2_3311	0.89	+0.1
3,826,672	+				+0.4
3,826,672	+				-1.0
3,826,673	-				+0.2
3,827,195	+		Pf6N2E2_3312	0.58	-0.2
3,827,195	+		Pf6N2E2_3312	0.58	-1.5
3,827,195	+		Pf6N2E2_3312	0.58	-0.2
3,827,196	-		Pf6N2E2_3312	0.58	-0.6
3,827,196	-		Pf6N2E2_3312	0.58	-0.7
3,827,243	+		Pf6N2E2_3312	0.63	+0.6
3,827,244	-		Pf6N2E2_3312	0.63	-0.1
3,827,450	+		Pf6N2E2_3312	0.86	-1.6
3,827,451	-		Pf6N2E2_3312	0.86	-0.4
3,827,451	-		Pf6N2E2_3312	0.86	-0.3
3,827,600	-				-0.8