Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3253

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
3,763,145	+		Pf6N2E2_3252	0.50	-1.4
3,763,145	+		Pf6N2E2_3252	0.50	-3.4
3,763,145	+		Pf6N2E2_3252	0.50	-3.0
3,763,145	+		Pf6N2E2_3252	0.50	-1.6
3,763,145	+		Pf6N2E2_3252	0.50	-0.6
3,763,145	+		Pf6N2E2_3252	0.50	-4.7
3,763,145	+		Pf6N2E2_3252	0.50	-3.3
3,763,145	+		Pf6N2E2_3252	0.50	-3.5
3,763,145	+		Pf6N2E2_3252	0.50	-3.9
3,763,145	+		Pf6N2E2_3252	0.50	-2.0
3,763,146	-		Pf6N2E2_3252	0.50	-3.0
3,763,146	-		Pf6N2E2_3252	0.50	-1.6
3,763,146	-		Pf6N2E2_3252	0.50	-2.6
3,763,146	-		Pf6N2E2_3252	0.50	-3.3
3,763,146	-		Pf6N2E2_3252	0.50	+1.4
3,763,379	+		Pf6N2E2_3252	0.68	-2.2
3,763,404	-		Pf6N2E2_3252	0.70	-0.2
3,763,404	-		Pf6N2E2_3252	0.70	+1.4
3,763,852	+				+0.4
3,763,853	-				+0.7
3,763,853	-				-0.8
3,763,889	+				+0.1
3,764,159	+		Pf6N2E2_3253	0.36	-4.7
3,764,159	+		Pf6N2E2_3253	0.36	-2.8
3,764,159	+		Pf6N2E2_3253	0.36	-3.3
3,764,160	-		Pf6N2E2_3253	0.36	-2.5
3,764,160	-		Pf6N2E2_3253	0.36	-3.9
3,764,160	-		Pf6N2E2_3253	0.36	-2.3
3,764,199	-		Pf6N2E2_3253	0.42	-0.9
3,764,201	+		Pf6N2E2_3253	0.42	-1.2
3,764,202	-		Pf6N2E2_3253	0.42	-3.7
3,764,202	-		Pf6N2E2_3253	0.42	-1.2
3,764,396	+		Pf6N2E2_3253	0.73	-3.3
3,764,396	+		Pf6N2E2_3253	0.73	-3.7
3,764,396	+		Pf6N2E2_3253	0.73	-3.9
3,764,396	+		Pf6N2E2_3253	0.73	+0.4
3,764,585	+				-3.1
3,764,593	+				-4.4
3,764,594	-				-0.4
3,764,594	-				-1.1
3,764,594	-				+0.5
3,764,594	-				+0.5
3,764,697	+				-1.7