Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3129 and Pf6N2E2_3130 are separated by 25 nucleotides Pf6N2E2_3130 and Pf6N2E2_3131 are separated by 72 nucleotides Pf6N2E2_3131 and Pf6N2E2_3132 are separated by 146 nucleotides Pf6N2E2_3132 and Pf6N2E2_3133 overlap by 38 nucleotides Pf6N2E2_3133 and Pf6N2E2_3134 are separated by 44 nucleotides
Pf6N2E2_3129 - Spermidine Putrescine ABC transporter permease component PotB (TC 3.A.1.11.1), at 3,620,673 to 3,621,620
_3129
Pf6N2E2_3130 - Spermidine Putrescine ABC transporter permease component potC (TC_3.A.1.11.1), at 3,621,646 to 3,622,428
_3130
Pf6N2E2_3131 - 4-aminobutyraldehyde dehydrogenase (EC 1.2.1.19), at 3,622,501 to 3,623,925
_3131
Pf6N2E2_3132 - FIG00954548: hypothetical protein, at 3,624,072 to 3,624,335
_3132
Pf6N2E2_3133 - Death on curing protein, Doc toxin, at 3,624,298 to 3,624,597
_3133
Pf6N2E2_3134 - hypothetical protein, at 3,624,642 to 3,624,827
_3134
Position (kb)
3622
3623
3624 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3621.576 kb on + strand at 3621.576 kb on + strand at 3621.576 kb on + strand at 3621.576 kb on + strand at 3621.576 kb on + strand at 3621.577 kb on - strand at 3621.577 kb on - strand at 3621.577 kb on - strand at 3621.580 kb on + strand at 3621.580 kb on + strand at 3621.580 kb on + strand at 3621.581 kb on - strand at 3621.581 kb on - strand at 3621.709 kb on + strand at 3621.919 kb on - strand, within Pf6N2E2_3130 at 3622.063 kb on + strand, within Pf6N2E2_3130 at 3622.157 kb on - strand, within Pf6N2E2_3130 at 3622.349 kb on + strand, within Pf6N2E2_3130 at 3622.349 kb on + strand, within Pf6N2E2_3130 at 3622.350 kb on - strand at 3622.378 kb on + strand at 3622.378 kb on + strand at 3622.379 kb on - strand at 3622.379 kb on - strand at 3622.381 kb on + strand at 3622.381 kb on + strand at 3622.381 kb on + strand at 3622.426 kb on + strand at 3622.426 kb on + strand at 3622.426 kb on + strand at 3622.427 kb on - strand at 3622.427 kb on - strand at 3622.876 kb on + strand, within Pf6N2E2_3131 at 3622.948 kb on + strand, within Pf6N2E2_3131 at 3622.948 kb on + strand, within Pf6N2E2_3131 at 3622.949 kb on - strand, within Pf6N2E2_3131 at 3623.001 kb on + strand, within Pf6N2E2_3131 at 3623.087 kb on + strand, within Pf6N2E2_3131 at 3623.088 kb on - strand, within Pf6N2E2_3131 at 3623.088 kb on - strand, within Pf6N2E2_3131 at 3623.320 kb on + strand, within Pf6N2E2_3131 at 3623.321 kb on - strand, within Pf6N2E2_3131 at 3623.359 kb on + strand, within Pf6N2E2_3131 at 3623.360 kb on - strand, within Pf6N2E2_3131 at 3623.360 kb on - strand, within Pf6N2E2_3131 at 3623.360 kb on - strand, within Pf6N2E2_3131 at 3623.377 kb on + strand, within Pf6N2E2_3131 at 3623.377 kb on + strand, within Pf6N2E2_3131 at 3623.378 kb on - strand, within Pf6N2E2_3131 at 3623.378 kb on - strand, within Pf6N2E2_3131 at 3623.425 kb on + strand, within Pf6N2E2_3131 at 3623.425 kb on + strand, within Pf6N2E2_3131 at 3623.425 kb on + strand, within Pf6N2E2_3131 at 3623.426 kb on - strand, within Pf6N2E2_3131 at 3623.529 kb on + strand, within Pf6N2E2_3131 at 3623.529 kb on + strand, within Pf6N2E2_3131 at 3623.530 kb on - strand, within Pf6N2E2_3131 at 3623.530 kb on - strand, within Pf6N2E2_3131 at 3623.530 kb on - strand, within Pf6N2E2_3131 at 3623.530 kb on - strand, within Pf6N2E2_3131 at 3623.684 kb on + strand, within Pf6N2E2_3131 at 3623.684 kb on + strand, within Pf6N2E2_3131 at 3623.684 kb on + strand, within Pf6N2E2_3131 at 3623.685 kb on - strand, within Pf6N2E2_3131 at 3623.685 kb on - strand, within Pf6N2E2_3131 at 3623.685 kb on - strand, within Pf6N2E2_3131 at 3623.779 kb on + strand, within Pf6N2E2_3131 at 3623.779 kb on + strand, within Pf6N2E2_3131 at 3623.780 kb on - strand, within Pf6N2E2_3131 at 3623.780 kb on - strand, within Pf6N2E2_3131 at 3623.875 kb on + strand at 3623.875 kb on + strand at 3623.875 kb on + strand at 3623.875 kb on + strand at 3623.875 kb on + strand at 3623.876 kb on - strand at 3623.876 kb on - strand at 3623.876 kb on - strand at 3623.876 kb on - strand at 3623.891 kb on - strand at 3623.980 kb on - strand at 3623.980 kb on - strand at 3623.980 kb on - strand at 3624.044 kb on + strand at 3624.051 kb on + strand at 3624.052 kb on - strand at 3624.052 kb on - strand at 3624.052 kb on - strand at 3624.052 kb on - strand at 3624.052 kb on - strand at 3624.053 kb on + strand at 3624.053 kb on + strand at 3624.054 kb on - strand at 3624.054 kb on - strand at 3624.163 kb on - strand, within Pf6N2E2_3132 at 3624.163 kb on - strand, within Pf6N2E2_3132 at 3624.190 kb on - strand, within Pf6N2E2_3132 at 3624.190 kb on - strand, within Pf6N2E2_3132 at 3624.190 kb on - strand, within Pf6N2E2_3132 at 3624.284 kb on + strand, within Pf6N2E2_3132 at 3624.284 kb on + strand, within Pf6N2E2_3132 at 3624.284 kb on + strand, within Pf6N2E2_3132 at 3624.311 kb on + strand at 3624.311 kb on + strand at 3624.312 kb on - strand at 3624.680 kb on - strand, within Pf6N2E2_3134 at 3624.816 kb on + strand at 3624.842 kb on + strand at 3624.842 kb on + strand at 3624.842 kb on + strand at 3624.907 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 3,621,576 + +0.3 3,621,576 + -0.1 3,621,576 + -0.9 3,621,576 + -1.0 3,621,576 + -0.2 3,621,577 - -1.2 3,621,577 - -0.1 3,621,577 - -0.4 3,621,580 + +0.0 3,621,580 + +0.1 3,621,580 + -0.5 3,621,581 - -0.6 3,621,581 - +0.4 3,621,709 + +0.3 3,621,919 - Pf6N2E2_3130 0.35 +1.9 3,622,063 + Pf6N2E2_3130 0.53 -0.6 3,622,157 - Pf6N2E2_3130 0.65 -2.6 3,622,349 + Pf6N2E2_3130 0.90 -0.1 3,622,349 + Pf6N2E2_3130 0.90 -0.0 3,622,350 - +0.4 3,622,378 + -0.6 3,622,378 + -0.4 3,622,379 - -0.1 3,622,379 - +0.5 3,622,381 + +1.5 3,622,381 + +0.1 3,622,381 + +1.5 3,622,426 + -0.2 3,622,426 + -0.1 3,622,426 + -1.2 3,622,427 - -0.7 3,622,427 - -0.1 3,622,876 + Pf6N2E2_3131 0.26 -2.0 3,622,948 + Pf6N2E2_3131 0.31 +1.9 3,622,948 + Pf6N2E2_3131 0.31 -0.9 3,622,949 - Pf6N2E2_3131 0.31 -0.6 3,623,001 + Pf6N2E2_3131 0.35 +0.5 3,623,087 + Pf6N2E2_3131 0.41 -2.9 3,623,088 - Pf6N2E2_3131 0.41 +0.3 3,623,088 - Pf6N2E2_3131 0.41 +0.4 3,623,320 + Pf6N2E2_3131 0.57 +0.0 3,623,321 - Pf6N2E2_3131 0.58 +0.5 3,623,359 + Pf6N2E2_3131 0.60 -0.2 3,623,360 - Pf6N2E2_3131 0.60 -1.9 3,623,360 - Pf6N2E2_3131 0.60 -0.2 3,623,360 - Pf6N2E2_3131 0.60 +0.5 3,623,377 + Pf6N2E2_3131 0.61 +1.3 3,623,377 + Pf6N2E2_3131 0.61 -0.1 3,623,378 - Pf6N2E2_3131 0.62 +0.1 3,623,378 - Pf6N2E2_3131 0.62 -0.1 3,623,425 + Pf6N2E2_3131 0.65 +0.6 3,623,425 + Pf6N2E2_3131 0.65 -1.5 3,623,425 + Pf6N2E2_3131 0.65 -0.4 3,623,426 - Pf6N2E2_3131 0.65 -0.7 3,623,529 + Pf6N2E2_3131 0.72 -2.0 3,623,529 + Pf6N2E2_3131 0.72 +0.2 3,623,530 - Pf6N2E2_3131 0.72 +2.5 3,623,530 - Pf6N2E2_3131 0.72 -0.1 3,623,530 - Pf6N2E2_3131 0.72 +0.2 3,623,530 - Pf6N2E2_3131 0.72 +0.1 3,623,684 + Pf6N2E2_3131 0.83 -0.1 3,623,684 + Pf6N2E2_3131 0.83 -0.4 3,623,684 + Pf6N2E2_3131 0.83 -0.6 3,623,685 - Pf6N2E2_3131 0.83 -0.8 3,623,685 - Pf6N2E2_3131 0.83 +0.6 3,623,685 - Pf6N2E2_3131 0.83 +1.5 3,623,779 + Pf6N2E2_3131 0.90 +0.4 3,623,779 + Pf6N2E2_3131 0.90 +2.1 3,623,780 - Pf6N2E2_3131 0.90 +1.2 3,623,780 - Pf6N2E2_3131 0.90 -0.0 3,623,875 + -1.9 3,623,875 + -0.1 3,623,875 + -1.2 3,623,875 + -0.6 3,623,875 + +0.8 3,623,876 - +0.3 3,623,876 - -0.5 3,623,876 - -0.5 3,623,876 - +0.6 3,623,891 - -0.3 3,623,980 - +0.2 3,623,980 - +3.1 3,623,980 - -0.1 3,624,044 + -0.2 3,624,051 + +0.1 3,624,052 - +0.1 3,624,052 - -2.8 3,624,052 - -0.0 3,624,052 - +0.5 3,624,052 - -1.3 3,624,053 + +0.8 3,624,053 + -2.4 3,624,054 - +0.3 3,624,054 - -0.4 3,624,163 - Pf6N2E2_3132 0.34 -2.8 3,624,163 - Pf6N2E2_3132 0.34 -1.4 3,624,190 - Pf6N2E2_3132 0.45 -0.5 3,624,190 - Pf6N2E2_3132 0.45 -0.5 3,624,190 - Pf6N2E2_3132 0.45 +0.5 3,624,284 + Pf6N2E2_3132 0.80 -0.9 3,624,284 + Pf6N2E2_3132 0.80 -0.1 3,624,284 + Pf6N2E2_3132 0.80 -1.3 3,624,311 + +0.4 3,624,311 + -0.7 3,624,312 - +0.1 3,624,680 - Pf6N2E2_3134 0.20 -0.1 3,624,816 + -0.1 3,624,842 + +0.0 3,624,842 + +1.5 3,624,842 + -0.5 3,624,907 - -0.4
Or see this region's nucleotide sequence