Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3127 and Pf6N2E2_3128 are separated by 229 nucleotides Pf6N2E2_3128 and Pf6N2E2_3129 are separated by 1 nucleotides Pf6N2E2_3129 and Pf6N2E2_3130 are separated by 25 nucleotides
Pf6N2E2_3127 - ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1), at 3,618,253 to 3,619,404
_3127
Pf6N2E2_3128 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 3,619,634 to 3,620,671
_3128
Pf6N2E2_3129 - Spermidine Putrescine ABC transporter permease component PotB (TC 3.A.1.11.1), at 3,620,673 to 3,621,620
_3129
Pf6N2E2_3130 - Spermidine Putrescine ABC transporter permease component potC (TC_3.A.1.11.1), at 3,621,646 to 3,622,428
_3130
Position (kb)
3619
3620
3621 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3618.649 kb on + strand, within Pf6N2E2_3127 at 3618.649 kb on + strand, within Pf6N2E2_3127 at 3618.649 kb on + strand, within Pf6N2E2_3127 at 3618.650 kb on - strand, within Pf6N2E2_3127 at 3618.650 kb on - strand, within Pf6N2E2_3127 at 3618.650 kb on - strand, within Pf6N2E2_3127 at 3618.709 kb on + strand, within Pf6N2E2_3127 at 3618.709 kb on + strand, within Pf6N2E2_3127 at 3618.709 kb on + strand, within Pf6N2E2_3127 at 3618.709 kb on + strand, within Pf6N2E2_3127 at 3618.710 kb on - strand, within Pf6N2E2_3127 at 3618.812 kb on - strand, within Pf6N2E2_3127 at 3618.812 kb on - strand, within Pf6N2E2_3127 at 3618.826 kb on + strand, within Pf6N2E2_3127 at 3618.826 kb on + strand, within Pf6N2E2_3127 at 3618.827 kb on - strand, within Pf6N2E2_3127 at 3618.847 kb on + strand, within Pf6N2E2_3127 at 3618.847 kb on + strand, within Pf6N2E2_3127 at 3618.847 kb on + strand, within Pf6N2E2_3127 at 3618.890 kb on - strand, within Pf6N2E2_3127 at 3618.910 kb on + strand, within Pf6N2E2_3127 at 3618.910 kb on + strand, within Pf6N2E2_3127 at 3618.910 kb on + strand, within Pf6N2E2_3127 at 3618.910 kb on + strand, within Pf6N2E2_3127 at 3618.910 kb on + strand, within Pf6N2E2_3127 at 3618.910 kb on + strand, within Pf6N2E2_3127 at 3618.911 kb on - strand, within Pf6N2E2_3127 at 3619.034 kb on - strand, within Pf6N2E2_3127 at 3619.034 kb on - strand, within Pf6N2E2_3127 at 3619.156 kb on + strand, within Pf6N2E2_3127 at 3619.156 kb on + strand, within Pf6N2E2_3127 at 3619.378 kb on + strand at 3619.378 kb on + strand at 3619.378 kb on + strand at 3619.378 kb on + strand at 3619.378 kb on + strand at 3619.378 kb on + strand at 3619.378 kb on + strand at 3619.379 kb on - strand at 3619.379 kb on - strand at 3619.379 kb on - strand at 3619.379 kb on - strand at 3619.412 kb on + strand at 3619.436 kb on + strand at 3619.662 kb on + strand at 3619.662 kb on + strand at 3619.853 kb on + strand, within Pf6N2E2_3128 at 3619.871 kb on - strand, within Pf6N2E2_3128 at 3619.871 kb on - strand, within Pf6N2E2_3128 at 3619.904 kb on - strand, within Pf6N2E2_3128 at 3619.904 kb on - strand, within Pf6N2E2_3128 at 3620.300 kb on + strand, within Pf6N2E2_3128 at 3620.300 kb on + strand, within Pf6N2E2_3128 at 3620.301 kb on - strand, within Pf6N2E2_3128 at 3620.301 kb on - strand, within Pf6N2E2_3128 at 3620.301 kb on - strand, within Pf6N2E2_3128 at 3620.301 kb on - strand, within Pf6N2E2_3128 at 3620.489 kb on + strand, within Pf6N2E2_3128 at 3620.510 kb on + strand, within Pf6N2E2_3128 at 3620.760 kb on + strand at 3620.761 kb on - strand at 3620.761 kb on - strand at 3620.761 kb on - strand at 3620.808 kb on + strand, within Pf6N2E2_3129 at 3620.809 kb on - strand, within Pf6N2E2_3129 at 3620.847 kb on + strand, within Pf6N2E2_3129 at 3620.848 kb on - strand, within Pf6N2E2_3129 at 3621.006 kb on + strand, within Pf6N2E2_3129 at 3621.006 kb on + strand, within Pf6N2E2_3129 at 3621.006 kb on + strand, within Pf6N2E2_3129 at 3621.007 kb on - strand, within Pf6N2E2_3129 at 3621.007 kb on - strand, within Pf6N2E2_3129 at 3621.009 kb on + strand, within Pf6N2E2_3129 at 3621.009 kb on + strand, within Pf6N2E2_3129 at 3621.009 kb on + strand, within Pf6N2E2_3129 at 3621.009 kb on + strand, within Pf6N2E2_3129 at 3621.009 kb on + strand, within Pf6N2E2_3129 at 3621.009 kb on + strand, within Pf6N2E2_3129 at 3621.009 kb on + strand, within Pf6N2E2_3129 at 3621.009 kb on + strand, within Pf6N2E2_3129 at 3621.009 kb on + strand, within Pf6N2E2_3129 at 3621.010 kb on - strand, within Pf6N2E2_3129 at 3621.010 kb on - strand, within Pf6N2E2_3129 at 3621.010 kb on - strand, within Pf6N2E2_3129 at 3621.010 kb on - strand, within Pf6N2E2_3129 at 3621.010 kb on - strand, within Pf6N2E2_3129 at 3621.010 kb on - strand, within Pf6N2E2_3129 at 3621.010 kb on - strand, within Pf6N2E2_3129 at 3621.010 kb on - strand, within Pf6N2E2_3129 at 3621.010 kb on - strand, within Pf6N2E2_3129 at 3621.010 kb on - strand, within Pf6N2E2_3129 at 3621.022 kb on - strand, within Pf6N2E2_3129 at 3621.051 kb on + strand, within Pf6N2E2_3129 at 3621.051 kb on + strand, within Pf6N2E2_3129 at 3621.052 kb on - strand, within Pf6N2E2_3129 at 3621.084 kb on + strand, within Pf6N2E2_3129 at 3621.084 kb on + strand, within Pf6N2E2_3129 at 3621.085 kb on - strand, within Pf6N2E2_3129 at 3621.085 kb on - strand, within Pf6N2E2_3129 at 3621.085 kb on - strand, within Pf6N2E2_3129 at 3621.085 kb on - strand, within Pf6N2E2_3129 at 3621.099 kb on + strand, within Pf6N2E2_3129 at 3621.100 kb on - strand, within Pf6N2E2_3129 at 3621.100 kb on - strand, within Pf6N2E2_3129 at 3621.100 kb on - strand, within Pf6N2E2_3129 at 3621.100 kb on - strand, within Pf6N2E2_3129 at 3621.255 kb on + strand, within Pf6N2E2_3129 at 3621.256 kb on - strand, within Pf6N2E2_3129 at 3621.256 kb on - strand, within Pf6N2E2_3129 at 3621.477 kb on + strand, within Pf6N2E2_3129 at 3621.477 kb on + strand, within Pf6N2E2_3129 at 3621.478 kb on - strand, within Pf6N2E2_3129 at 3621.478 kb on - strand, within Pf6N2E2_3129 at 3621.499 kb on - strand, within Pf6N2E2_3129 at 3621.576 kb on + strand at 3621.576 kb on + strand at 3621.576 kb on + strand at 3621.576 kb on + strand at 3621.576 kb on + strand at 3621.577 kb on - strand at 3621.577 kb on - strand at 3621.577 kb on - strand at 3621.580 kb on + strand at 3621.580 kb on + strand at 3621.580 kb on + strand at 3621.581 kb on - strand at 3621.581 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 3,618,649 + Pf6N2E2_3127 0.34 +0.7 3,618,649 + Pf6N2E2_3127 0.34 -0.5 3,618,649 + Pf6N2E2_3127 0.34 +0.3 3,618,650 - Pf6N2E2_3127 0.34 -1.2 3,618,650 - Pf6N2E2_3127 0.34 -0.6 3,618,650 - Pf6N2E2_3127 0.34 -0.6 3,618,709 + Pf6N2E2_3127 0.40 +0.3 3,618,709 + Pf6N2E2_3127 0.40 -0.6 3,618,709 + Pf6N2E2_3127 0.40 +0.7 3,618,709 + Pf6N2E2_3127 0.40 +1.5 3,618,710 - Pf6N2E2_3127 0.40 +0.4 3,618,812 - Pf6N2E2_3127 0.49 +0.1 3,618,812 - Pf6N2E2_3127 0.49 -0.1 3,618,826 + Pf6N2E2_3127 0.50 -0.3 3,618,826 + Pf6N2E2_3127 0.50 -0.5 3,618,827 - Pf6N2E2_3127 0.50 +0.7 3,618,847 + Pf6N2E2_3127 0.52 +0.5 3,618,847 + Pf6N2E2_3127 0.52 +0.2 3,618,847 + Pf6N2E2_3127 0.52 +0.6 3,618,890 - Pf6N2E2_3127 0.55 -1.2 3,618,910 + Pf6N2E2_3127 0.57 -1.1 3,618,910 + Pf6N2E2_3127 0.57 +0.7 3,618,910 + Pf6N2E2_3127 0.57 +0.1 3,618,910 + Pf6N2E2_3127 0.57 -0.4 3,618,910 + Pf6N2E2_3127 0.57 +0.1 3,618,910 + Pf6N2E2_3127 0.57 +0.5 3,618,911 - Pf6N2E2_3127 0.57 -0.5 3,619,034 - Pf6N2E2_3127 0.68 +0.6 3,619,034 - Pf6N2E2_3127 0.68 -0.8 3,619,156 + Pf6N2E2_3127 0.78 +1.5 3,619,156 + Pf6N2E2_3127 0.78 +0.3 3,619,378 + +0.1 3,619,378 + -0.3 3,619,378 + -0.3 3,619,378 + -1.8 3,619,378 + +0.2 3,619,378 + -1.4 3,619,378 + -0.6 3,619,379 - -0.6 3,619,379 - +1.5 3,619,379 - +0.0 3,619,379 - +0.2 3,619,412 + -0.2 3,619,436 + -0.7 3,619,662 + +0.6 3,619,662 + +1.5 3,619,853 + Pf6N2E2_3128 0.21 +0.8 3,619,871 - Pf6N2E2_3128 0.23 +0.2 3,619,871 - Pf6N2E2_3128 0.23 -0.8 3,619,904 - Pf6N2E2_3128 0.26 +0.3 3,619,904 - Pf6N2E2_3128 0.26 -0.2 3,620,300 + Pf6N2E2_3128 0.64 -1.9 3,620,300 + Pf6N2E2_3128 0.64 +0.1 3,620,301 - Pf6N2E2_3128 0.64 -0.7 3,620,301 - Pf6N2E2_3128 0.64 +0.5 3,620,301 - Pf6N2E2_3128 0.64 -0.3 3,620,301 - Pf6N2E2_3128 0.64 -1.1 3,620,489 + Pf6N2E2_3128 0.82 +0.1 3,620,510 + Pf6N2E2_3128 0.84 -1.3 3,620,760 + +0.9 3,620,761 - -0.5 3,620,761 - -0.3 3,620,761 - +0.5 3,620,808 + Pf6N2E2_3129 0.14 -0.3 3,620,809 - Pf6N2E2_3129 0.14 +0.3 3,620,847 + Pf6N2E2_3129 0.18 -0.4 3,620,848 - Pf6N2E2_3129 0.18 -1.1 3,621,006 + Pf6N2E2_3129 0.35 -0.3 3,621,006 + Pf6N2E2_3129 0.35 -1.5 3,621,006 + Pf6N2E2_3129 0.35 +0.5 3,621,007 - Pf6N2E2_3129 0.35 -0.2 3,621,007 - Pf6N2E2_3129 0.35 +1.2 3,621,009 + Pf6N2E2_3129 0.35 -0.2 3,621,009 + Pf6N2E2_3129 0.35 +0.2 3,621,009 + Pf6N2E2_3129 0.35 -0.6 3,621,009 + Pf6N2E2_3129 0.35 +1.0 3,621,009 + Pf6N2E2_3129 0.35 +1.5 3,621,009 + Pf6N2E2_3129 0.35 -0.5 3,621,009 + Pf6N2E2_3129 0.35 -0.4 3,621,009 + Pf6N2E2_3129 0.35 +0.2 3,621,009 + Pf6N2E2_3129 0.35 -1.5 3,621,010 - Pf6N2E2_3129 0.36 +0.5 3,621,010 - Pf6N2E2_3129 0.36 +0.3 3,621,010 - Pf6N2E2_3129 0.36 -0.9 3,621,010 - Pf6N2E2_3129 0.36 -0.0 3,621,010 - Pf6N2E2_3129 0.36 +0.4 3,621,010 - Pf6N2E2_3129 0.36 -0.7 3,621,010 - Pf6N2E2_3129 0.36 -0.1 3,621,010 - Pf6N2E2_3129 0.36 +0.9 3,621,010 - Pf6N2E2_3129 0.36 -0.2 3,621,010 - Pf6N2E2_3129 0.36 +0.9 3,621,022 - Pf6N2E2_3129 0.37 -1.4 3,621,051 + Pf6N2E2_3129 0.40 +0.2 3,621,051 + Pf6N2E2_3129 0.40 +0.1 3,621,052 - Pf6N2E2_3129 0.40 -0.5 3,621,084 + Pf6N2E2_3129 0.43 +0.3 3,621,084 + Pf6N2E2_3129 0.43 -0.1 3,621,085 - Pf6N2E2_3129 0.43 +0.5 3,621,085 - Pf6N2E2_3129 0.43 +0.7 3,621,085 - Pf6N2E2_3129 0.43 -0.4 3,621,085 - Pf6N2E2_3129 0.43 -0.1 3,621,099 + Pf6N2E2_3129 0.45 -0.7 3,621,100 - Pf6N2E2_3129 0.45 -0.2 3,621,100 - Pf6N2E2_3129 0.45 -0.6 3,621,100 - Pf6N2E2_3129 0.45 +2.1 3,621,100 - Pf6N2E2_3129 0.45 -0.3 3,621,255 + Pf6N2E2_3129 0.61 +0.7 3,621,256 - Pf6N2E2_3129 0.61 -0.8 3,621,256 - Pf6N2E2_3129 0.61 -0.0 3,621,477 + Pf6N2E2_3129 0.85 -0.1 3,621,477 + Pf6N2E2_3129 0.85 +0.1 3,621,478 - Pf6N2E2_3129 0.85 +1.5 3,621,478 - Pf6N2E2_3129 0.85 -0.1 3,621,499 - Pf6N2E2_3129 0.87 +0.3 3,621,576 + +0.3 3,621,576 + -0.1 3,621,576 + -0.9 3,621,576 + -1.0 3,621,576 + -0.2 3,621,577 - -1.2 3,621,577 - -0.1 3,621,577 - -0.4 3,621,580 + +0.0 3,621,580 + +0.1 3,621,580 + -0.5 3,621,581 - -0.6 3,621,581 - +0.4
Or see this region's nucleotide sequence