Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3038 and Pf6N2E2_3039 are separated by 856 nucleotides Pf6N2E2_3039 and Pf6N2E2_3040 overlap by 1 nucleotides Pf6N2E2_3040 and Pf6N2E2_3041 overlap by 13 nucleotides
Pf6N2E2_3038 - Lipoprotein NlpD, at 3,522,793 to 3,522,993
_3038
Pf6N2E2_3039 - Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77), at 3,523,850 to 3,524,527
_3039
Pf6N2E2_3040 - 5-nucleotidase SurE (EC 3.1.3.5), at 3,524,527 to 3,525,276
_3040
Pf6N2E2_3041 - tRNA pseudouridine 13 synthase (EC 4.2.1.-), at 3,525,264 to 3,526,322
_3041
Position (kb)
3523
3524
3525 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3523.698 kb on - strand at 3523.704 kb on - strand at 3523.704 kb on - strand at 3523.704 kb on - strand at 3523.704 kb on - strand at 3523.704 kb on - strand at 3523.704 kb on - strand at 3523.706 kb on - strand at 3523.706 kb on - strand at 3523.728 kb on - strand at 3523.842 kb on - strand at 3523.844 kb on - strand at 3523.844 kb on - strand at 3523.844 kb on - strand at 3523.844 kb on - strand at 3524.055 kb on + strand, within Pf6N2E2_3039 at 3524.056 kb on - strand, within Pf6N2E2_3039 at 3524.157 kb on - strand, within Pf6N2E2_3039 at 3524.298 kb on + strand, within Pf6N2E2_3039 at 3524.298 kb on + strand, within Pf6N2E2_3039 at 3524.298 kb on + strand, within Pf6N2E2_3039 at 3524.298 kb on + strand, within Pf6N2E2_3039 at 3524.298 kb on + strand, within Pf6N2E2_3039 at 3524.298 kb on + strand, within Pf6N2E2_3039 at 3524.298 kb on + strand, within Pf6N2E2_3039 at 3524.298 kb on + strand, within Pf6N2E2_3039 at 3524.298 kb on + strand, within Pf6N2E2_3039 at 3524.298 kb on + strand, within Pf6N2E2_3039 at 3524.299 kb on - strand, within Pf6N2E2_3039 at 3524.299 kb on - strand, within Pf6N2E2_3039 at 3524.299 kb on - strand, within Pf6N2E2_3039 at 3524.299 kb on - strand, within Pf6N2E2_3039 at 3524.299 kb on - strand, within Pf6N2E2_3039 at 3524.299 kb on - strand, within Pf6N2E2_3039 at 3524.299 kb on - strand, within Pf6N2E2_3039 at 3524.299 kb on - strand, within Pf6N2E2_3039 at 3524.396 kb on - strand, within Pf6N2E2_3039 at 3524.442 kb on + strand, within Pf6N2E2_3039 at 3524.443 kb on - strand, within Pf6N2E2_3039 at 3524.580 kb on + strand at 3524.581 kb on - strand at 3524.612 kb on + strand, within Pf6N2E2_3040 at 3524.612 kb on + strand, within Pf6N2E2_3040 at 3524.612 kb on + strand, within Pf6N2E2_3040 at 3524.612 kb on + strand, within Pf6N2E2_3040 at 3524.612 kb on + strand, within Pf6N2E2_3040 at 3524.612 kb on + strand, within Pf6N2E2_3040 at 3524.612 kb on + strand, within Pf6N2E2_3040 at 3524.612 kb on + strand, within Pf6N2E2_3040 at 3524.613 kb on - strand, within Pf6N2E2_3040 at 3524.613 kb on - strand, within Pf6N2E2_3040 at 3524.613 kb on - strand, within Pf6N2E2_3040 at 3524.613 kb on - strand, within Pf6N2E2_3040 at 3524.613 kb on - strand, within Pf6N2E2_3040 at 3524.613 kb on - strand, within Pf6N2E2_3040 at 3524.613 kb on - strand, within Pf6N2E2_3040 at 3524.613 kb on - strand, within Pf6N2E2_3040 at 3524.613 kb on - strand, within Pf6N2E2_3040 at 3524.972 kb on + strand, within Pf6N2E2_3040 at 3524.973 kb on - strand, within Pf6N2E2_3040 at 3524.973 kb on - strand, within Pf6N2E2_3040 at 3525.009 kb on + strand, within Pf6N2E2_3040 at 3525.082 kb on - strand, within Pf6N2E2_3040 at 3525.113 kb on + strand, within Pf6N2E2_3040 at 3525.219 kb on - strand at 3525.219 kb on - strand at 3525.270 kb on + strand at 3525.270 kb on + strand at 3525.271 kb on - strand at 3525.271 kb on - strand at 3525.326 kb on - strand at 3525.453 kb on + strand, within Pf6N2E2_3041 at 3525.454 kb on - strand, within Pf6N2E2_3041 at 3525.454 kb on - strand, within Pf6N2E2_3041
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 3,523,698 - -1.2 3,523,704 - +0.5 3,523,704 - -2.6 3,523,704 - +0.1 3,523,704 - -0.7 3,523,704 - -0.8 3,523,704 - -0.3 3,523,706 - +1.5 3,523,706 - -0.0 3,523,728 - +1.1 3,523,842 - -0.2 3,523,844 - -0.4 3,523,844 - +0.3 3,523,844 - -1.3 3,523,844 - -0.5 3,524,055 + Pf6N2E2_3039 0.30 -2.0 3,524,056 - Pf6N2E2_3039 0.30 -1.2 3,524,157 - Pf6N2E2_3039 0.45 -0.0 3,524,298 + Pf6N2E2_3039 0.66 -2.8 3,524,298 + Pf6N2E2_3039 0.66 -2.2 3,524,298 + Pf6N2E2_3039 0.66 -2.5 3,524,298 + Pf6N2E2_3039 0.66 -0.6 3,524,298 + Pf6N2E2_3039 0.66 -2.6 3,524,298 + Pf6N2E2_3039 0.66 -2.0 3,524,298 + Pf6N2E2_3039 0.66 -1.6 3,524,298 + Pf6N2E2_3039 0.66 -2.2 3,524,298 + Pf6N2E2_3039 0.66 -0.9 3,524,298 + Pf6N2E2_3039 0.66 +1.5 3,524,299 - Pf6N2E2_3039 0.66 -0.8 3,524,299 - Pf6N2E2_3039 0.66 -1.8 3,524,299 - Pf6N2E2_3039 0.66 +0.5 3,524,299 - Pf6N2E2_3039 0.66 -0.1 3,524,299 - Pf6N2E2_3039 0.66 -1.3 3,524,299 - Pf6N2E2_3039 0.66 -1.7 3,524,299 - Pf6N2E2_3039 0.66 -1.8 3,524,299 - Pf6N2E2_3039 0.66 -0.7 3,524,396 - Pf6N2E2_3039 0.81 -0.1 3,524,442 + Pf6N2E2_3039 0.87 -0.9 3,524,443 - Pf6N2E2_3039 0.87 -2.1 3,524,580 + -1.4 3,524,581 - -1.2 3,524,612 + Pf6N2E2_3040 0.11 -2.5 3,524,612 + Pf6N2E2_3040 0.11 -2.8 3,524,612 + Pf6N2E2_3040 0.11 -1.1 3,524,612 + Pf6N2E2_3040 0.11 -0.8 3,524,612 + Pf6N2E2_3040 0.11 -1.3 3,524,612 + Pf6N2E2_3040 0.11 -1.8 3,524,612 + Pf6N2E2_3040 0.11 -1.1 3,524,612 + Pf6N2E2_3040 0.11 -0.4 3,524,613 - Pf6N2E2_3040 0.11 -0.3 3,524,613 - Pf6N2E2_3040 0.11 -0.6 3,524,613 - Pf6N2E2_3040 0.11 +0.4 3,524,613 - Pf6N2E2_3040 0.11 +0.3 3,524,613 - Pf6N2E2_3040 0.11 +0.5 3,524,613 - Pf6N2E2_3040 0.11 +0.6 3,524,613 - Pf6N2E2_3040 0.11 +0.5 3,524,613 - Pf6N2E2_3040 0.11 +0.2 3,524,613 - Pf6N2E2_3040 0.11 -0.0 3,524,972 + Pf6N2E2_3040 0.59 -2.4 3,524,973 - Pf6N2E2_3040 0.59 -1.6 3,524,973 - Pf6N2E2_3040 0.59 +0.2 3,525,009 + Pf6N2E2_3040 0.64 -0.6 3,525,082 - Pf6N2E2_3040 0.74 +0.4 3,525,113 + Pf6N2E2_3040 0.78 -3.2 3,525,219 - -0.1 3,525,219 - -0.4 3,525,270 + -1.5 3,525,270 + -0.8 3,525,271 - +0.2 3,525,271 - -0.1 3,525,326 - +0.3 3,525,453 + Pf6N2E2_3041 0.18 -0.2 3,525,454 - Pf6N2E2_3041 0.18 -1.5 3,525,454 - Pf6N2E2_3041 0.18 -2.1
Or see this region's nucleotide sequence