Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_291

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
359,403	+		Pf6N2E2_289	0.83	+1.6
359,403	+		Pf6N2E2_289	0.83	-0.8
359,404	-		Pf6N2E2_289	0.83	-2.4
359,404	-		Pf6N2E2_289	0.83	-2.0
359,404	-		Pf6N2E2_289	0.83	+1.6
359,541	+				-2.5
359,541	+				-2.5
359,541	+				-2.8
359,541	+				-1.8
359,541	+				+0.6
359,541	+				-0.9
359,542	-				-2.5
359,542	-				-1.9
359,542	-				-1.7
359,542	-				-1.4
359,542	-				-2.2
359,542	-				-0.8
359,542	-				-2.8
359,619	+				-0.9
359,619	+				-2.8
359,619	+				-1.0
359,620	-				-1.1
359,620	-				-1.7
359,620	-				-1.0
359,620	-				-0.4
359,620	-				-0.7
359,620	-				-1.3
359,836	+		Pf6N2E2_290	0.51	+2.6
359,836	+		Pf6N2E2_290	0.51	-0.8
359,837	-		Pf6N2E2_290	0.51	-2.0
359,837	-		Pf6N2E2_290	0.51	-1.3
359,838	+		Pf6N2E2_290	0.51	+1.6
359,838	+		Pf6N2E2_290	0.51	+1.6
359,838	+		Pf6N2E2_290	0.51	+0.4
359,839	-		Pf6N2E2_290	0.52	-0.8
359,862	+		Pf6N2E2_290	0.60	-0.4
359,968	+				+0.6
359,968	+				+0.3
360,007	-				-2.6
360,007	-				-0.5
360,007	-				+0.6
360,007	-				-0.4
360,029	+				-0.0
360,030	-				-2.3
360,040	+				-0.9
360,040	+				-0.5
360,040	+				-0.8
360,041	-				-1.6
360,041	-				-0.3
360,041	-				-0.0
360,047	-				-1.6
360,083	-				-1.2
360,083	-				-0.8
360,092	-				-1.2
360,092	-				-2.9
360,165	+				+1.6
360,165	+				-0.4
360,176	+				-1.6
360,176	+				-1.1
360,177	-				-0.9
360,177	-				+0.1
360,221	-				-2.0
360,221	-				-2.0
360,248	-				-1.5
360,298	+				-1.7
360,298	+				-1.2
360,299	-				-0.1
360,315	+				-1.0
360,315	+				-0.0
360,315	+				-1.9
360,315	+				-3.2
360,316	-				-1.4
360,342	+		Pf6N2E2_291	0.12	+0.1
360,342	+		Pf6N2E2_291	0.12	-1.7
360,342	+		Pf6N2E2_291	0.12	-2.2
360,431	+		Pf6N2E2_291	0.48	-1.3
360,431	+		Pf6N2E2_291	0.48	-1.4
360,432	-		Pf6N2E2_291	0.49	-1.6
360,448	+		Pf6N2E2_291	0.55	-0.2
360,448	+		Pf6N2E2_291	0.55	+0.1
360,449	-		Pf6N2E2_291	0.56	-2.2
360,530	-		Pf6N2E2_291	0.89	-1.4
360,573	+				-0.8
360,586	+				-1.0
360,586	+				+1.6
360,586	+				-1.1
360,586	+				-0.2
360,587	-				+1.6
360,587	-				-3.7
360,587	-				-1.0
360,591	+				-0.0
360,688	-				-1.2
360,870	+		Pf6N2E2_292	0.12	-0.0
360,870	+		Pf6N2E2_292	0.12	-1.6
360,870	+		Pf6N2E2_292	0.12	+1.6
360,870	+		Pf6N2E2_292	0.12	-0.8
360,870	+		Pf6N2E2_292	0.12	-1.1
360,871	-		Pf6N2E2_292	0.13	-1.9
360,871	-		Pf6N2E2_292	0.13	-2.7
360,871	-		Pf6N2E2_292	0.13	-2.5
360,871	-		Pf6N2E2_292	0.13	-1.8
360,995	+		Pf6N2E2_292	0.18	-3.0
360,996	-		Pf6N2E2_292	0.18	-1.5
361,058	-		Pf6N2E2_292	0.21	-1.8
361,113	-		Pf6N2E2_292	0.23	-0.0
361,334	+		Pf6N2E2_292	0.33	-1.5
361,334	+		Pf6N2E2_292	0.33	-2.0
361,334	+		Pf6N2E2_292	0.33	-0.4
361,335	-		Pf6N2E2_292	0.33	-1.2