Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2770 and Pf6N2E2_2771 overlap by 8 nucleotides Pf6N2E2_2771 and Pf6N2E2_2772 are separated by 244 nucleotides
Pf6N2E2_2770 - Tricarboxylate transport membrane protein TctA, at 3,257,724 to 3,259,238
_2770
Pf6N2E2_2771 - Ammonia monooxygenase, at 3,259,231 to 3,260,271
_2771
Pf6N2E2_2772 - Uracil-DNA glycosylase, family 1, at 3,260,516 to 3,261,208
_2772
Position (kb)
3259
3260
3261 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3258.564 kb on + strand, within Pf6N2E2_2770 at 3258.564 kb on + strand, within Pf6N2E2_2770 at 3258.564 kb on + strand, within Pf6N2E2_2770 at 3258.564 kb on + strand, within Pf6N2E2_2770 at 3258.564 kb on + strand, within Pf6N2E2_2770 at 3258.565 kb on - strand, within Pf6N2E2_2770 at 3258.565 kb on - strand, within Pf6N2E2_2770 at 3258.565 kb on - strand, within Pf6N2E2_2770 at 3258.565 kb on - strand, within Pf6N2E2_2770 at 3258.565 kb on - strand, within Pf6N2E2_2770 at 3258.565 kb on - strand, within Pf6N2E2_2770 at 3258.565 kb on - strand, within Pf6N2E2_2770 at 3258.565 kb on - strand, within Pf6N2E2_2770 at 3258.565 kb on - strand, within Pf6N2E2_2770 at 3258.565 kb on - strand, within Pf6N2E2_2770 at 3258.566 kb on + strand, within Pf6N2E2_2770 at 3258.567 kb on - strand, within Pf6N2E2_2770 at 3258.567 kb on - strand, within Pf6N2E2_2770 at 3258.567 kb on - strand, within Pf6N2E2_2770 at 3258.745 kb on - strand, within Pf6N2E2_2770 at 3258.939 kb on + strand, within Pf6N2E2_2770 at 3258.939 kb on + strand, within Pf6N2E2_2770 at 3258.939 kb on + strand, within Pf6N2E2_2770 at 3259.000 kb on - strand, within Pf6N2E2_2770 at 3259.368 kb on + strand, within Pf6N2E2_2771 at 3259.368 kb on + strand, within Pf6N2E2_2771 at 3259.369 kb on - strand, within Pf6N2E2_2771 at 3259.369 kb on - strand, within Pf6N2E2_2771 at 3259.404 kb on + strand, within Pf6N2E2_2771 at 3259.404 kb on + strand, within Pf6N2E2_2771 at 3259.404 kb on + strand, within Pf6N2E2_2771 at 3259.405 kb on - strand, within Pf6N2E2_2771 at 3259.405 kb on - strand, within Pf6N2E2_2771 at 3259.405 kb on - strand, within Pf6N2E2_2771 at 3259.647 kb on + strand, within Pf6N2E2_2771 at 3259.648 kb on - strand, within Pf6N2E2_2771 at 3259.648 kb on - strand, within Pf6N2E2_2771 at 3259.688 kb on + strand, within Pf6N2E2_2771 at 3259.689 kb on - strand, within Pf6N2E2_2771 at 3259.689 kb on - strand, within Pf6N2E2_2771 at 3259.717 kb on + strand, within Pf6N2E2_2771 at 3259.718 kb on - strand, within Pf6N2E2_2771 at 3259.718 kb on - strand, within Pf6N2E2_2771 at 3259.866 kb on - strand, within Pf6N2E2_2771 at 3259.866 kb on - strand, within Pf6N2E2_2771 at 3259.866 kb on - strand, within Pf6N2E2_2771 at 3259.866 kb on - strand, within Pf6N2E2_2771 at 3260.060 kb on + strand, within Pf6N2E2_2771 at 3260.090 kb on + strand, within Pf6N2E2_2771 at 3260.090 kb on + strand, within Pf6N2E2_2771 at 3260.091 kb on - strand, within Pf6N2E2_2771 at 3260.091 kb on - strand, within Pf6N2E2_2771 at 3260.091 kb on - strand, within Pf6N2E2_2771 at 3260.091 kb on - strand, within Pf6N2E2_2771 at 3260.091 kb on - strand, within Pf6N2E2_2771 at 3260.091 kb on - strand, within Pf6N2E2_2771 at 3260.109 kb on + strand, within Pf6N2E2_2771 at 3260.110 kb on - strand, within Pf6N2E2_2771 at 3260.267 kb on + strand at 3260.267 kb on + strand at 3260.267 kb on + strand at 3260.267 kb on + strand at 3260.268 kb on - strand at 3260.268 kb on - strand at 3260.269 kb on + strand at 3260.269 kb on + strand at 3260.270 kb on - strand at 3260.517 kb on + strand at 3260.517 kb on + strand at 3260.517 kb on + strand at 3260.517 kb on + strand at 3260.518 kb on - strand at 3260.705 kb on + strand, within Pf6N2E2_2772 at 3260.821 kb on + strand, within Pf6N2E2_2772 at 3260.821 kb on + strand, within Pf6N2E2_2772 at 3260.821 kb on + strand, within Pf6N2E2_2772 at 3260.822 kb on - strand, within Pf6N2E2_2772 at 3260.822 kb on - strand, within Pf6N2E2_2772 at 3260.913 kb on + strand, within Pf6N2E2_2772 at 3260.913 kb on + strand, within Pf6N2E2_2772 at 3260.913 kb on + strand, within Pf6N2E2_2772 at 3260.913 kb on + strand, within Pf6N2E2_2772 at 3260.914 kb on - strand, within Pf6N2E2_2772 at 3260.914 kb on - strand, within Pf6N2E2_2772 at 3260.914 kb on - strand, within Pf6N2E2_2772 at 3260.914 kb on - strand, within Pf6N2E2_2772 at 3260.917 kb on + strand, within Pf6N2E2_2772 at 3261.100 kb on - strand, within Pf6N2E2_2772 at 3261.100 kb on - strand, within Pf6N2E2_2772 at 3261.111 kb on - strand, within Pf6N2E2_2772 at 3261.135 kb on + strand, within Pf6N2E2_2772 at 3261.135 kb on + strand, within Pf6N2E2_2772 at 3261.135 kb on + strand, within Pf6N2E2_2772 at 3261.135 kb on + strand, within Pf6N2E2_2772 at 3261.135 kb on + strand, within Pf6N2E2_2772 at 3261.136 kb on - strand, within Pf6N2E2_2772 at 3261.136 kb on - strand, within Pf6N2E2_2772 at 3261.136 kb on - strand, within Pf6N2E2_2772 at 3261.136 kb on - strand, within Pf6N2E2_2772 at 3261.191 kb on - strand at 3261.251 kb on + strand at 3261.251 kb on + strand at 3261.251 kb on + strand at 3261.252 kb on - strand at 3261.252 kb on - strand at 3261.252 kb on - strand at 3261.252 kb on - strand at 3261.252 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 3,258,564 + Pf6N2E2_2770 0.55 +0.2 3,258,564 + Pf6N2E2_2770 0.55 -0.4 3,258,564 + Pf6N2E2_2770 0.55 +1.0 3,258,564 + Pf6N2E2_2770 0.55 +1.1 3,258,564 + Pf6N2E2_2770 0.55 +0.2 3,258,565 - Pf6N2E2_2770 0.56 -0.1 3,258,565 - Pf6N2E2_2770 0.56 -0.2 3,258,565 - Pf6N2E2_2770 0.56 -2.4 3,258,565 - Pf6N2E2_2770 0.56 +1.0 3,258,565 - Pf6N2E2_2770 0.56 +0.1 3,258,565 - Pf6N2E2_2770 0.56 +0.8 3,258,565 - Pf6N2E2_2770 0.56 +1.5 3,258,565 - Pf6N2E2_2770 0.56 -0.2 3,258,565 - Pf6N2E2_2770 0.56 +0.0 3,258,565 - Pf6N2E2_2770 0.56 -0.5 3,258,566 + Pf6N2E2_2770 0.56 -0.5 3,258,567 - Pf6N2E2_2770 0.56 -0.8 3,258,567 - Pf6N2E2_2770 0.56 +0.6 3,258,567 - Pf6N2E2_2770 0.56 -0.1 3,258,745 - Pf6N2E2_2770 0.67 -0.1 3,258,939 + Pf6N2E2_2770 0.80 -0.4 3,258,939 + Pf6N2E2_2770 0.80 -0.6 3,258,939 + Pf6N2E2_2770 0.80 +0.0 3,259,000 - Pf6N2E2_2770 0.84 +0.2 3,259,368 + Pf6N2E2_2771 0.13 -0.4 3,259,368 + Pf6N2E2_2771 0.13 -1.2 3,259,369 - Pf6N2E2_2771 0.13 -1.6 3,259,369 - Pf6N2E2_2771 0.13 -1.3 3,259,404 + Pf6N2E2_2771 0.17 -0.8 3,259,404 + Pf6N2E2_2771 0.17 -0.5 3,259,404 + Pf6N2E2_2771 0.17 -1.1 3,259,405 - Pf6N2E2_2771 0.17 -1.0 3,259,405 - Pf6N2E2_2771 0.17 -0.5 3,259,405 - Pf6N2E2_2771 0.17 -0.1 3,259,647 + Pf6N2E2_2771 0.40 -0.1 3,259,648 - Pf6N2E2_2771 0.40 -0.5 3,259,648 - Pf6N2E2_2771 0.40 -1.6 3,259,688 + Pf6N2E2_2771 0.44 -0.4 3,259,689 - Pf6N2E2_2771 0.44 +1.5 3,259,689 - Pf6N2E2_2771 0.44 -0.2 3,259,717 + Pf6N2E2_2771 0.47 +0.0 3,259,718 - Pf6N2E2_2771 0.47 +0.9 3,259,718 - Pf6N2E2_2771 0.47 +1.5 3,259,866 - Pf6N2E2_2771 0.61 -1.6 3,259,866 - Pf6N2E2_2771 0.61 +0.1 3,259,866 - Pf6N2E2_2771 0.61 +0.0 3,259,866 - Pf6N2E2_2771 0.61 +0.7 3,260,060 + Pf6N2E2_2771 0.80 -3.1 3,260,090 + Pf6N2E2_2771 0.83 -0.1 3,260,090 + Pf6N2E2_2771 0.83 -0.5 3,260,091 - Pf6N2E2_2771 0.83 -1.1 3,260,091 - Pf6N2E2_2771 0.83 -0.7 3,260,091 - Pf6N2E2_2771 0.83 -0.8 3,260,091 - Pf6N2E2_2771 0.83 +0.3 3,260,091 - Pf6N2E2_2771 0.83 +0.5 3,260,091 - Pf6N2E2_2771 0.83 +0.1 3,260,109 + Pf6N2E2_2771 0.84 +0.2 3,260,110 - Pf6N2E2_2771 0.84 -0.0 3,260,267 + -0.1 3,260,267 + +1.5 3,260,267 + +0.0 3,260,267 + -1.4 3,260,268 - +0.3 3,260,268 - -3.2 3,260,269 + -0.6 3,260,269 + -0.1 3,260,270 - +0.1 3,260,517 + -0.8 3,260,517 + +0.3 3,260,517 + -2.0 3,260,517 + +0.7 3,260,518 - +1.5 3,260,705 + Pf6N2E2_2772 0.27 -0.9 3,260,821 + Pf6N2E2_2772 0.44 -0.1 3,260,821 + Pf6N2E2_2772 0.44 +0.7 3,260,821 + Pf6N2E2_2772 0.44 +0.2 3,260,822 - Pf6N2E2_2772 0.44 +0.8 3,260,822 - Pf6N2E2_2772 0.44 -1.1 3,260,913 + Pf6N2E2_2772 0.57 +0.9 3,260,913 + Pf6N2E2_2772 0.57 +1.5 3,260,913 + Pf6N2E2_2772 0.57 -1.1 3,260,913 + Pf6N2E2_2772 0.57 +0.5 3,260,914 - Pf6N2E2_2772 0.57 -0.6 3,260,914 - Pf6N2E2_2772 0.57 +0.1 3,260,914 - Pf6N2E2_2772 0.57 -0.0 3,260,914 - Pf6N2E2_2772 0.57 +2.1 3,260,917 + Pf6N2E2_2772 0.58 -1.7 3,261,100 - Pf6N2E2_2772 0.84 +1.1 3,261,100 - Pf6N2E2_2772 0.84 +0.3 3,261,111 - Pf6N2E2_2772 0.86 +0.5 3,261,135 + Pf6N2E2_2772 0.89 -0.5 3,261,135 + Pf6N2E2_2772 0.89 +0.8 3,261,135 + Pf6N2E2_2772 0.89 -0.3 3,261,135 + Pf6N2E2_2772 0.89 -0.1 3,261,135 + Pf6N2E2_2772 0.89 -1.0 3,261,136 - Pf6N2E2_2772 0.89 -0.8 3,261,136 - Pf6N2E2_2772 0.89 -0.3 3,261,136 - Pf6N2E2_2772 0.89 +0.2 3,261,136 - Pf6N2E2_2772 0.89 +0.1 3,261,191 - +0.2 3,261,251 + +0.3 3,261,251 + +0.0 3,261,251 + -0.2 3,261,252 - +0.8 3,261,252 - -0.4 3,261,252 - +1.0 3,261,252 - -1.1 3,261,252 - -0.6
Or see this region's nucleotide sequence