Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2750 and Pf6N2E2_2751 are separated by 29 nucleotides Pf6N2E2_2751 and Pf6N2E2_2752 are separated by 98 nucleotides Pf6N2E2_2752 and Pf6N2E2_2753 are separated by 63 nucleotides
Pf6N2E2_2750 - Putative permease PerM (= YfgO), at 3,242,055 to 3,243,125
_2750
Pf6N2E2_2751 - Rhodanese-like domain protein, at 3,243,155 to 3,243,394
_2751
Pf6N2E2_2752 - Exported zinc metalloprotease YfgC precursor, at 3,243,493 to 3,244,926
_2752
Pf6N2E2_2753 - HtrA protease/chaperone protein, at 3,244,990 to 3,246,420
_2753
Position (kb)
3243
3244
3245 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3242.632 kb on + strand, within Pf6N2E2_2750 at 3242.633 kb on - strand, within Pf6N2E2_2750 at 3242.633 kb on - strand, within Pf6N2E2_2750 at 3242.633 kb on - strand, within Pf6N2E2_2750 at 3242.872 kb on - strand, within Pf6N2E2_2750 at 3242.977 kb on + strand, within Pf6N2E2_2750 at 3243.092 kb on - strand at 3243.092 kb on - strand at 3243.380 kb on + strand at 3243.380 kb on + strand at 3243.427 kb on + strand at 3243.428 kb on - strand at 3243.432 kb on + strand at 3243.432 kb on + strand at 3243.432 kb on + strand at 3243.432 kb on + strand at 3243.433 kb on - strand at 3243.433 kb on - strand at 3243.433 kb on - strand at 3243.459 kb on + strand at 3243.464 kb on + strand at 3243.464 kb on + strand at 3243.464 kb on + strand at 3243.464 kb on + strand at 3243.465 kb on - strand at 3243.481 kb on - strand at 3243.688 kb on + strand, within Pf6N2E2_2752 at 3243.688 kb on + strand, within Pf6N2E2_2752 at 3243.689 kb on - strand, within Pf6N2E2_2752 at 3243.706 kb on + strand, within Pf6N2E2_2752 at 3243.706 kb on + strand, within Pf6N2E2_2752 at 3243.707 kb on - strand, within Pf6N2E2_2752 at 3243.853 kb on + strand, within Pf6N2E2_2752 at 3243.854 kb on - strand, within Pf6N2E2_2752 at 3244.117 kb on + strand, within Pf6N2E2_2752 at 3244.321 kb on + strand, within Pf6N2E2_2752 at 3244.321 kb on + strand, within Pf6N2E2_2752 at 3244.321 kb on + strand, within Pf6N2E2_2752 at 3244.322 kb on - strand, within Pf6N2E2_2752 at 3244.322 kb on - strand, within Pf6N2E2_2752 at 3244.399 kb on + strand, within Pf6N2E2_2752 at 3244.495 kb on + strand, within Pf6N2E2_2752 at 3244.495 kb on + strand, within Pf6N2E2_2752 at 3244.495 kb on + strand, within Pf6N2E2_2752 at 3244.495 kb on + strand, within Pf6N2E2_2752 at 3244.495 kb on + strand, within Pf6N2E2_2752 at 3244.495 kb on + strand, within Pf6N2E2_2752 at 3244.495 kb on + strand, within Pf6N2E2_2752 at 3244.496 kb on - strand, within Pf6N2E2_2752 at 3244.496 kb on - strand, within Pf6N2E2_2752 at 3244.496 kb on - strand, within Pf6N2E2_2752 at 3244.496 kb on - strand, within Pf6N2E2_2752 at 3244.496 kb on - strand, within Pf6N2E2_2752 at 3244.496 kb on - strand, within Pf6N2E2_2752 at 3244.496 kb on - strand, within Pf6N2E2_2752 at 3244.496 kb on - strand, within Pf6N2E2_2752 at 3244.496 kb on - strand, within Pf6N2E2_2752 at 3244.496 kb on - strand, within Pf6N2E2_2752 at 3244.496 kb on - strand, within Pf6N2E2_2752 at 3244.579 kb on + strand, within Pf6N2E2_2752 at 3244.592 kb on - strand, within Pf6N2E2_2752 at 3244.705 kb on + strand, within Pf6N2E2_2752 at 3244.705 kb on + strand, within Pf6N2E2_2752 at 3244.705 kb on + strand, within Pf6N2E2_2752 at 3244.771 kb on + strand, within Pf6N2E2_2752 at 3244.943 kb on - strand at 3244.943 kb on - strand at 3245.130 kb on + strand at 3245.131 kb on - strand at 3245.131 kb on - strand at 3245.410 kb on + strand, within Pf6N2E2_2753 at 3245.410 kb on + strand, within Pf6N2E2_2753 at 3245.410 kb on + strand, within Pf6N2E2_2753 at 3245.709 kb on - strand, within Pf6N2E2_2753 at 3245.811 kb on - strand, within Pf6N2E2_2753
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 3,242,632 + Pf6N2E2_2750 0.54 +0.4 3,242,633 - Pf6N2E2_2750 0.54 -0.5 3,242,633 - Pf6N2E2_2750 0.54 +1.5 3,242,633 - Pf6N2E2_2750 0.54 -2.7 3,242,872 - Pf6N2E2_2750 0.76 -1.0 3,242,977 + Pf6N2E2_2750 0.86 +0.5 3,243,092 - +0.2 3,243,092 - +1.4 3,243,380 + -2.8 3,243,380 + -1.8 3,243,427 + +0.3 3,243,428 - +0.1 3,243,432 + -1.0 3,243,432 + -0.8 3,243,432 + +0.2 3,243,432 + +1.5 3,243,433 - -0.4 3,243,433 - -0.3 3,243,433 - +0.4 3,243,459 + -1.0 3,243,464 + +1.1 3,243,464 + +0.4 3,243,464 + -0.2 3,243,464 + +0.5 3,243,465 - +1.5 3,243,481 - +1.5 3,243,688 + Pf6N2E2_2752 0.14 +1.5 3,243,688 + Pf6N2E2_2752 0.14 +1.5 3,243,689 - Pf6N2E2_2752 0.14 +1.5 3,243,706 + Pf6N2E2_2752 0.15 -1.2 3,243,706 + Pf6N2E2_2752 0.15 -1.8 3,243,707 - Pf6N2E2_2752 0.15 -3.3 3,243,853 + Pf6N2E2_2752 0.25 +0.5 3,243,854 - Pf6N2E2_2752 0.25 -1.8 3,244,117 + Pf6N2E2_2752 0.44 +1.5 3,244,321 + Pf6N2E2_2752 0.58 -2.5 3,244,321 + Pf6N2E2_2752 0.58 -0.1 3,244,321 + Pf6N2E2_2752 0.58 -2.8 3,244,322 - Pf6N2E2_2752 0.58 -2.3 3,244,322 - Pf6N2E2_2752 0.58 +1.5 3,244,399 + Pf6N2E2_2752 0.63 -1.3 3,244,495 + Pf6N2E2_2752 0.70 +1.5 3,244,495 + Pf6N2E2_2752 0.70 -2.8 3,244,495 + Pf6N2E2_2752 0.70 +1.5 3,244,495 + Pf6N2E2_2752 0.70 +1.5 3,244,495 + Pf6N2E2_2752 0.70 +1.5 3,244,495 + Pf6N2E2_2752 0.70 -2.4 3,244,495 + Pf6N2E2_2752 0.70 +1.5 3,244,496 - Pf6N2E2_2752 0.70 +1.5 3,244,496 - Pf6N2E2_2752 0.70 +1.5 3,244,496 - Pf6N2E2_2752 0.70 +1.5 3,244,496 - Pf6N2E2_2752 0.70 -0.5 3,244,496 - Pf6N2E2_2752 0.70 +1.5 3,244,496 - Pf6N2E2_2752 0.70 +1.5 3,244,496 - Pf6N2E2_2752 0.70 +1.5 3,244,496 - Pf6N2E2_2752 0.70 +1.5 3,244,496 - Pf6N2E2_2752 0.70 +1.5 3,244,496 - Pf6N2E2_2752 0.70 +1.5 3,244,496 - Pf6N2E2_2752 0.70 -3.3 3,244,579 + Pf6N2E2_2752 0.76 +0.5 3,244,592 - Pf6N2E2_2752 0.77 +1.5 3,244,705 + Pf6N2E2_2752 0.85 +1.5 3,244,705 + Pf6N2E2_2752 0.85 +1.5 3,244,705 + Pf6N2E2_2752 0.85 +1.5 3,244,771 + Pf6N2E2_2752 0.89 -1.3 3,244,943 - -0.5 3,244,943 - +0.5 3,245,130 + -0.7 3,245,131 - +0.2 3,245,131 - +1.5 3,245,410 + Pf6N2E2_2753 0.29 -0.5 3,245,410 + Pf6N2E2_2753 0.29 -0.1 3,245,410 + Pf6N2E2_2753 0.29 +1.2 3,245,709 - Pf6N2E2_2753 0.50 -0.6 3,245,811 - Pf6N2E2_2753 0.57 -1.3
Or see this region's nucleotide sequence