Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2674

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
3,161,984	-		Pf6N2E2_2673	0.77	-0.8
3,162,043	+		Pf6N2E2_2673	0.80	+1.5
3,162,086	+		Pf6N2E2_2673	0.81	+0.5
3,162,119	+		Pf6N2E2_2673	0.83	-2.8
3,162,120	-		Pf6N2E2_2673	0.83	+0.2
3,162,183	+		Pf6N2E2_2673	0.85	+1.5
3,162,184	-		Pf6N2E2_2673	0.85	-0.8
3,162,184	-		Pf6N2E2_2673	0.85	+1.5
3,162,277	+		Pf6N2E2_2673	0.89	+0.3
3,162,278	-		Pf6N2E2_2673	0.89	-0.1
3,162,281	+		Pf6N2E2_2673	0.89	+0.5
3,162,296	-		Pf6N2E2_2673	0.90	+0.3
3,162,438	+				+1.7
3,162,439	-				+0.5
3,162,554	+				+1.5
3,162,598	-				+0.6
3,162,613	+				+1.1
3,162,614	-				-0.4
3,162,617	+				-0.2
3,162,683	+				-0.2
3,162,683	+				+0.9
3,162,684	-				-0.2
3,162,684	-				+1.5
3,162,684	-				-0.4
3,162,697	+				+0.6
3,162,698	-				-0.1
3,162,713	+				-2.4
3,162,713	+				-1.3
3,162,713	+				-0.5
3,162,713	+				-1.1
3,162,713	+				-0.1
3,162,713	+				+0.5
3,162,714	-				-1.1
3,162,751	+				-1.2
3,162,752	-				-0.1
3,162,780	-				+0.7
3,162,780	-				-0.0
3,162,792	-				+0.5
3,162,907	+				-1.9
3,162,908	-				+0.5
3,163,151	+		Pf6N2E2_2674	0.83	-1.0
3,163,204	-				+0.3
3,163,292	+				+1.4
3,163,293	-				-0.9
3,163,293	-				+0.5
3,163,373	+				-0.2
3,163,373	+				-0.0
3,163,373	+				-0.5
3,163,373	+				+0.5
3,163,373	+				+0.3
3,163,374	-				+0.2
3,163,393	-				-1.3
3,163,657	-		Pf6N2E2_2675	0.16	+0.7