Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2654 and Pf6N2E2_2655 are separated by 346 nucleotides Pf6N2E2_2655 and Pf6N2E2_2656 are separated by 9 nucleotides Pf6N2E2_2656 and Pf6N2E2_2657 are separated by 52 nucleotides
Pf6N2E2_2654 - Transcriptional regulator GbuR, at 3,126,600 to 3,127,493
_2654
Pf6N2E2_2655 - Agmatinase (EC 3.5.3.11), at 3,127,840 to 3,128,790
_2655
Pf6N2E2_2656 - Undecaprenyl pyrophosphate synthase, at 3,128,800 to 3,129,207
_2656
Pf6N2E2_2657 - Cytosine/purine/uracil/thiamine/allantoin permease family protein, at 3,129,260 to 3,130,771
_2657
Position (kb)
3127
3128
3129 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3126.967 kb on + strand, within Pf6N2E2_2654 at 3126.968 kb on - strand, within Pf6N2E2_2654 at 3126.988 kb on + strand, within Pf6N2E2_2654 at 3126.989 kb on - strand, within Pf6N2E2_2654 at 3127.000 kb on + strand, within Pf6N2E2_2654 at 3127.000 kb on + strand, within Pf6N2E2_2654 at 3127.000 kb on + strand, within Pf6N2E2_2654 at 3127.001 kb on - strand, within Pf6N2E2_2654 at 3127.237 kb on + strand, within Pf6N2E2_2654 at 3127.237 kb on + strand, within Pf6N2E2_2654 at 3127.238 kb on - strand, within Pf6N2E2_2654 at 3127.375 kb on + strand, within Pf6N2E2_2654 at 3127.375 kb on + strand, within Pf6N2E2_2654 at 3127.375 kb on + strand, within Pf6N2E2_2654 at 3127.376 kb on - strand, within Pf6N2E2_2654 at 3127.376 kb on - strand, within Pf6N2E2_2654 at 3127.376 kb on - strand, within Pf6N2E2_2654 at 3127.376 kb on - strand, within Pf6N2E2_2654 at 3127.376 kb on - strand, within Pf6N2E2_2654 at 3127.376 kb on - strand, within Pf6N2E2_2654 at 3127.498 kb on + strand at 3127.498 kb on + strand at 3127.498 kb on + strand at 3127.498 kb on + strand at 3127.519 kb on + strand at 3127.520 kb on - strand at 3127.520 kb on - strand at 3127.520 kb on - strand at 3127.520 kb on - strand at 3127.520 kb on - strand at 3127.580 kb on - strand at 3127.586 kb on - strand at 3127.695 kb on + strand at 3127.696 kb on - strand at 3127.753 kb on + strand at 3127.754 kb on - strand at 3127.754 kb on - strand at 3127.758 kb on - strand at 3127.817 kb on - strand at 3127.817 kb on - strand at 3127.817 kb on - strand at 3127.817 kb on - strand at 3128.051 kb on - strand, within Pf6N2E2_2655 at 3128.051 kb on - strand, within Pf6N2E2_2655 at 3128.051 kb on - strand, within Pf6N2E2_2655 at 3128.164 kb on + strand, within Pf6N2E2_2655 at 3128.165 kb on - strand, within Pf6N2E2_2655 at 3128.545 kb on + strand, within Pf6N2E2_2655 at 3128.545 kb on + strand, within Pf6N2E2_2655 at 3128.546 kb on - strand, within Pf6N2E2_2655 at 3128.546 kb on - strand, within Pf6N2E2_2655 at 3128.687 kb on + strand, within Pf6N2E2_2655 at 3128.688 kb on - strand, within Pf6N2E2_2655 at 3128.688 kb on - strand, within Pf6N2E2_2655 at 3128.750 kb on - strand at 3128.875 kb on + strand, within Pf6N2E2_2656 at 3128.875 kb on + strand, within Pf6N2E2_2656 at 3128.875 kb on + strand, within Pf6N2E2_2656 at 3128.951 kb on - strand, within Pf6N2E2_2656 at 3128.951 kb on - strand, within Pf6N2E2_2656 at 3129.149 kb on + strand, within Pf6N2E2_2656 at 3129.150 kb on - strand, within Pf6N2E2_2656 at 3129.205 kb on + strand at 3129.243 kb on + strand at 3129.243 kb on + strand at 3129.265 kb on + strand at 3129.265 kb on + strand at 3129.265 kb on + strand at 3129.265 kb on + strand at 3129.265 kb on + strand at 3129.266 kb on - strand at 3129.266 kb on - strand at 3129.266 kb on - strand at 3129.266 kb on - strand at 3129.266 kb on - strand at 3129.266 kb on - strand at 3129.266 kb on - strand at 3129.272 kb on - strand at 3129.383 kb on - strand at 3129.749 kb on + strand, within Pf6N2E2_2657 at 3129.750 kb on - strand, within Pf6N2E2_2657 at 3129.750 kb on - strand, within Pf6N2E2_2657 at 3129.750 kb on - strand, within Pf6N2E2_2657 at 3129.789 kb on + strand, within Pf6N2E2_2657
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 3,126,967 + Pf6N2E2_2654 0.41 -1.4 3,126,968 - Pf6N2E2_2654 0.41 -0.5 3,126,988 + Pf6N2E2_2654 0.43 +1.0 3,126,989 - Pf6N2E2_2654 0.44 -1.4 3,127,000 + Pf6N2E2_2654 0.45 -2.4 3,127,000 + Pf6N2E2_2654 0.45 +1.0 3,127,000 + Pf6N2E2_2654 0.45 -0.2 3,127,001 - Pf6N2E2_2654 0.45 -0.4 3,127,237 + Pf6N2E2_2654 0.71 +0.5 3,127,237 + Pf6N2E2_2654 0.71 -0.8 3,127,238 - Pf6N2E2_2654 0.71 +0.1 3,127,375 + Pf6N2E2_2654 0.87 -0.4 3,127,375 + Pf6N2E2_2654 0.87 -0.1 3,127,375 + Pf6N2E2_2654 0.87 +0.5 3,127,376 - Pf6N2E2_2654 0.87 +0.4 3,127,376 - Pf6N2E2_2654 0.87 +2.5 3,127,376 - Pf6N2E2_2654 0.87 +0.7 3,127,376 - Pf6N2E2_2654 0.87 +0.1 3,127,376 - Pf6N2E2_2654 0.87 +0.4 3,127,376 - Pf6N2E2_2654 0.87 +0.6 3,127,498 + +0.4 3,127,498 + +0.3 3,127,498 + -0.1 3,127,498 + -0.3 3,127,519 + +0.1 3,127,520 - +1.5 3,127,520 - -0.4 3,127,520 - -1.2 3,127,520 - -0.2 3,127,520 - +0.2 3,127,580 - -0.2 3,127,586 - +0.1 3,127,695 + -0.3 3,127,696 - -0.5 3,127,753 + -0.2 3,127,754 - -0.4 3,127,754 - -1.1 3,127,758 - +0.5 3,127,817 - -0.8 3,127,817 - +0.6 3,127,817 - -0.1 3,127,817 - -0.3 3,128,051 - Pf6N2E2_2655 0.22 +0.6 3,128,051 - Pf6N2E2_2655 0.22 +1.5 3,128,051 - Pf6N2E2_2655 0.22 +0.8 3,128,164 + Pf6N2E2_2655 0.34 +0.5 3,128,165 - Pf6N2E2_2655 0.34 -1.2 3,128,545 + Pf6N2E2_2655 0.74 +0.9 3,128,545 + Pf6N2E2_2655 0.74 -1.0 3,128,546 - Pf6N2E2_2655 0.74 -0.4 3,128,546 - Pf6N2E2_2655 0.74 +0.8 3,128,687 + Pf6N2E2_2655 0.89 +0.1 3,128,688 - Pf6N2E2_2655 0.89 -0.3 3,128,688 - Pf6N2E2_2655 0.89 -0.2 3,128,750 - -0.3 3,128,875 + Pf6N2E2_2656 0.18 -0.0 3,128,875 + Pf6N2E2_2656 0.18 -0.2 3,128,875 + Pf6N2E2_2656 0.18 +0.3 3,128,951 - Pf6N2E2_2656 0.37 +0.6 3,128,951 - Pf6N2E2_2656 0.37 +0.5 3,129,149 + Pf6N2E2_2656 0.86 +0.7 3,129,150 - Pf6N2E2_2656 0.86 -0.9 3,129,205 + -0.9 3,129,243 + +0.5 3,129,243 + -1.3 3,129,265 + +0.3 3,129,265 + +1.5 3,129,265 + +0.6 3,129,265 + +1.7 3,129,265 + -0.7 3,129,266 - +0.0 3,129,266 - -1.6 3,129,266 - -1.3 3,129,266 - +1.5 3,129,266 - -0.3 3,129,266 - +0.1 3,129,266 - +0.2 3,129,272 - +0.9 3,129,383 - +0.9 3,129,749 + Pf6N2E2_2657 0.32 +0.2 3,129,750 - Pf6N2E2_2657 0.32 -0.3 3,129,750 - Pf6N2E2_2657 0.32 -0.4 3,129,750 - Pf6N2E2_2657 0.32 -1.6 3,129,789 + Pf6N2E2_2657 0.35 -0.7
Or see this region's nucleotide sequence