Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2655

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
3,126,967	+		Pf6N2E2_2654	0.41	-1.4
3,126,968	-		Pf6N2E2_2654	0.41	-0.5
3,126,988	+		Pf6N2E2_2654	0.43	+1.0
3,126,989	-		Pf6N2E2_2654	0.44	-1.4
3,127,000	+		Pf6N2E2_2654	0.45	-2.4
3,127,000	+		Pf6N2E2_2654	0.45	+1.0
3,127,000	+		Pf6N2E2_2654	0.45	-0.2
3,127,001	-		Pf6N2E2_2654	0.45	-0.4
3,127,237	+		Pf6N2E2_2654	0.71	+0.5
3,127,237	+		Pf6N2E2_2654	0.71	-0.8
3,127,238	-		Pf6N2E2_2654	0.71	+0.1
3,127,375	+		Pf6N2E2_2654	0.87	-0.4
3,127,375	+		Pf6N2E2_2654	0.87	-0.1
3,127,375	+		Pf6N2E2_2654	0.87	+0.5
3,127,376	-		Pf6N2E2_2654	0.87	+0.4
3,127,376	-		Pf6N2E2_2654	0.87	+2.5
3,127,376	-		Pf6N2E2_2654	0.87	+0.7
3,127,376	-		Pf6N2E2_2654	0.87	+0.1
3,127,376	-		Pf6N2E2_2654	0.87	+0.4
3,127,376	-		Pf6N2E2_2654	0.87	+0.6
3,127,498	+				+0.4
3,127,498	+				+0.3
3,127,498	+				-0.1
3,127,498	+				-0.3
3,127,519	+				+0.1
3,127,520	-				+1.5
3,127,520	-				-0.4
3,127,520	-				-1.2
3,127,520	-				-0.2
3,127,520	-				+0.2
3,127,580	-				-0.2
3,127,586	-				+0.1
3,127,695	+				-0.3
3,127,696	-				-0.5
3,127,753	+				-0.2
3,127,754	-				-0.4
3,127,754	-				-1.1
3,127,758	-				+0.5
3,127,817	-				-0.8
3,127,817	-				+0.6
3,127,817	-				-0.1
3,127,817	-				-0.3
3,128,051	-		Pf6N2E2_2655	0.22	+0.6
3,128,051	-		Pf6N2E2_2655	0.22	+1.5
3,128,051	-		Pf6N2E2_2655	0.22	+0.8
3,128,164	+		Pf6N2E2_2655	0.34	+0.5
3,128,165	-		Pf6N2E2_2655	0.34	-1.2
3,128,545	+		Pf6N2E2_2655	0.74	+0.9
3,128,545	+		Pf6N2E2_2655	0.74	-1.0
3,128,546	-		Pf6N2E2_2655	0.74	-0.4
3,128,546	-		Pf6N2E2_2655	0.74	+0.8
3,128,687	+		Pf6N2E2_2655	0.89	+0.1
3,128,688	-		Pf6N2E2_2655	0.89	-0.3
3,128,688	-		Pf6N2E2_2655	0.89	-0.2
3,128,750	-				-0.3
3,128,875	+		Pf6N2E2_2656	0.18	-0.0
3,128,875	+		Pf6N2E2_2656	0.18	-0.2
3,128,875	+		Pf6N2E2_2656	0.18	+0.3
3,128,951	-		Pf6N2E2_2656	0.37	+0.6
3,128,951	-		Pf6N2E2_2656	0.37	+0.5
3,129,149	+		Pf6N2E2_2656	0.86	+0.7
3,129,150	-		Pf6N2E2_2656	0.86	-0.9
3,129,205	+				-0.9
3,129,243	+				+0.5
3,129,243	+				-1.3
3,129,265	+				+0.3
3,129,265	+				+1.5
3,129,265	+				+0.6
3,129,265	+				+1.7
3,129,265	+				-0.7
3,129,266	-				+0.0
3,129,266	-				-1.6
3,129,266	-				-1.3
3,129,266	-				+1.5
3,129,266	-				-0.3
3,129,266	-				+0.1
3,129,266	-				+0.2
3,129,272	-				+0.9
3,129,383	-				+0.9
3,129,749	+		Pf6N2E2_2657	0.32	+0.2
3,129,750	-		Pf6N2E2_2657	0.32	-0.3
3,129,750	-		Pf6N2E2_2657	0.32	-0.4
3,129,750	-		Pf6N2E2_2657	0.32	-1.6
3,129,789	+		Pf6N2E2_2657	0.35	-0.7