Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2561 and Pf6N2E2_2562 overlap by 4 nucleotides Pf6N2E2_2562 and Pf6N2E2_2563 are separated by 252 nucleotides Pf6N2E2_2563 and Pf6N2E2_2564 are separated by 1 nucleotides Pf6N2E2_2564 and Pf6N2E2_2565 are separated by 72 nucleotides
Pf6N2E2_2561 - FIG00955434: hypothetical protein, at 3,019,831 to 3,021,402
_2561
Pf6N2E2_2562 - FlhB domain protein, at 3,021,399 to 3,021,728
_2562
Pf6N2E2_2563 - FIG00953562: hypothetical protein, at 3,021,981 to 3,022,376
_2563
Pf6N2E2_2564 - Positive regulator of CheA protein activity (CheW), at 3,022,378 to 3,022,863
_2564
Pf6N2E2_2565 - CheW domain protein, at 3,022,936 to 3,023,874
_2565
Position (kb)
3022
3023 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3021.723 kb on + strand at 3021.724 kb on - strand at 3021.928 kb on + strand at 3021.928 kb on + strand at 3021.928 kb on + strand at 3022.001 kb on + strand at 3022.001 kb on + strand at 3022.001 kb on + strand at 3022.001 kb on + strand at 3022.001 kb on + strand at 3022.002 kb on - strand at 3022.002 kb on - strand at 3022.002 kb on - strand at 3022.302 kb on + strand, within Pf6N2E2_2563 at 3022.302 kb on + strand, within Pf6N2E2_2563 at 3022.302 kb on + strand, within Pf6N2E2_2563 at 3022.302 kb on + strand, within Pf6N2E2_2563 at 3022.302 kb on + strand, within Pf6N2E2_2563 at 3022.302 kb on + strand, within Pf6N2E2_2563 at 3022.302 kb on + strand, within Pf6N2E2_2563 at 3022.302 kb on + strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.304 kb on + strand, within Pf6N2E2_2563 at 3022.304 kb on + strand, within Pf6N2E2_2563 at 3022.304 kb on + strand, within Pf6N2E2_2563 at 3022.304 kb on + strand, within Pf6N2E2_2563 at 3022.305 kb on - strand, within Pf6N2E2_2563 at 3022.305 kb on - strand, within Pf6N2E2_2563 at 3022.305 kb on - strand, within Pf6N2E2_2563 at 3022.305 kb on - strand, within Pf6N2E2_2563 at 3022.305 kb on - strand, within Pf6N2E2_2563 at 3022.306 kb on + strand, within Pf6N2E2_2563 at 3022.306 kb on + strand, within Pf6N2E2_2563 at 3022.306 kb on + strand, within Pf6N2E2_2563 at 3022.306 kb on + strand, within Pf6N2E2_2563 at 3022.307 kb on - strand, within Pf6N2E2_2563 at 3022.307 kb on - strand, within Pf6N2E2_2563 at 3022.307 kb on - strand, within Pf6N2E2_2563 at 3022.307 kb on - strand, within Pf6N2E2_2563 at 3022.363 kb on - strand at 3022.363 kb on - strand at 3022.363 kb on - strand at 3022.384 kb on - strand at 3022.384 kb on - strand at 3022.384 kb on - strand at 3022.539 kb on - strand, within Pf6N2E2_2564 at 3022.706 kb on + strand, within Pf6N2E2_2564 at 3022.707 kb on - strand, within Pf6N2E2_2564 at 3022.707 kb on - strand, within Pf6N2E2_2564 at 3022.741 kb on - strand, within Pf6N2E2_2564 at 3022.742 kb on + strand, within Pf6N2E2_2564 at 3022.742 kb on + strand, within Pf6N2E2_2564 at 3022.781 kb on + strand, within Pf6N2E2_2564 at 3022.781 kb on + strand, within Pf6N2E2_2564 at 3022.782 kb on - strand, within Pf6N2E2_2564 at 3022.782 kb on - strand, within Pf6N2E2_2564 at 3022.854 kb on + strand at 3022.854 kb on + strand at 3022.863 kb on + strand at 3022.864 kb on - strand at 3022.900 kb on + strand at 3022.900 kb on + strand at 3022.900 kb on + strand at 3022.900 kb on + strand at 3022.900 kb on + strand at 3022.900 kb on + strand at 3022.901 kb on - strand at 3022.901 kb on - strand at 3022.901 kb on - strand at 3022.937 kb on + strand at 3022.937 kb on + strand at 3023.037 kb on + strand, within Pf6N2E2_2565 at 3023.037 kb on + strand, within Pf6N2E2_2565 at 3023.037 kb on + strand, within Pf6N2E2_2565 at 3023.037 kb on + strand, within Pf6N2E2_2565 at 3023.037 kb on + strand, within Pf6N2E2_2565 at 3023.037 kb on + strand, within Pf6N2E2_2565 at 3023.037 kb on + strand, within Pf6N2E2_2565 at 3023.037 kb on + strand, within Pf6N2E2_2565 at 3023.037 kb on + strand, within Pf6N2E2_2565 at 3023.037 kb on + strand, within Pf6N2E2_2565 at 3023.037 kb on + strand, within Pf6N2E2_2565 at 3023.037 kb on + strand, within Pf6N2E2_2565 at 3023.038 kb on - strand, within Pf6N2E2_2565 at 3023.038 kb on - strand, within Pf6N2E2_2565 at 3023.038 kb on - strand, within Pf6N2E2_2565 at 3023.038 kb on - strand, within Pf6N2E2_2565 at 3023.038 kb on - strand, within Pf6N2E2_2565 at 3023.038 kb on - strand, within Pf6N2E2_2565 at 3023.038 kb on - strand, within Pf6N2E2_2565 at 3023.038 kb on - strand, within Pf6N2E2_2565 at 3023.038 kb on - strand, within Pf6N2E2_2565 at 3023.052 kb on + strand, within Pf6N2E2_2565 at 3023.157 kb on - strand, within Pf6N2E2_2565 at 3023.177 kb on + strand, within Pf6N2E2_2565 at 3023.177 kb on + strand, within Pf6N2E2_2565 at 3023.178 kb on - strand, within Pf6N2E2_2565 at 3023.178 kb on - strand, within Pf6N2E2_2565 at 3023.606 kb on - strand, within Pf6N2E2_2565 at 3023.760 kb on - strand, within Pf6N2E2_2565 at 3023.760 kb on - strand, within Pf6N2E2_2565 at 3023.798 kb on - strand at 3023.811 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 3,021,723 + -0.6 3,021,724 - +0.2 3,021,928 + -0.7 3,021,928 + -0.0 3,021,928 + +0.1 3,022,001 + +0.4 3,022,001 + +1.2 3,022,001 + -0.5 3,022,001 + +1.2 3,022,001 + +0.7 3,022,002 - +0.4 3,022,002 - -0.1 3,022,002 - +1.0 3,022,302 + Pf6N2E2_2563 0.81 +0.6 3,022,302 + Pf6N2E2_2563 0.81 +0.5 3,022,302 + Pf6N2E2_2563 0.81 +0.1 3,022,302 + Pf6N2E2_2563 0.81 +1.3 3,022,302 + Pf6N2E2_2563 0.81 +1.0 3,022,302 + Pf6N2E2_2563 0.81 +1.5 3,022,302 + Pf6N2E2_2563 0.81 +1.1 3,022,302 + Pf6N2E2_2563 0.81 +0.1 3,022,303 - Pf6N2E2_2563 0.81 +1.0 3,022,303 - Pf6N2E2_2563 0.81 +1.0 3,022,303 - Pf6N2E2_2563 0.81 +0.9 3,022,303 - Pf6N2E2_2563 0.81 +0.9 3,022,303 - Pf6N2E2_2563 0.81 +1.2 3,022,303 - Pf6N2E2_2563 0.81 +0.7 3,022,303 - Pf6N2E2_2563 0.81 +1.1 3,022,303 - Pf6N2E2_2563 0.81 +2.5 3,022,303 - Pf6N2E2_2563 0.81 +1.1 3,022,303 - Pf6N2E2_2563 0.81 +0.6 3,022,303 - Pf6N2E2_2563 0.81 +0.9 3,022,303 - Pf6N2E2_2563 0.81 +0.7 3,022,303 - Pf6N2E2_2563 0.81 +1.2 3,022,303 - Pf6N2E2_2563 0.81 +0.6 3,022,303 - Pf6N2E2_2563 0.81 +0.7 3,022,303 - Pf6N2E2_2563 0.81 +0.9 3,022,303 - Pf6N2E2_2563 0.81 +1.0 3,022,303 - Pf6N2E2_2563 0.81 +0.7 3,022,303 - Pf6N2E2_2563 0.81 +0.4 3,022,303 - Pf6N2E2_2563 0.81 +0.9 3,022,303 - Pf6N2E2_2563 0.81 +1.8 3,022,303 - Pf6N2E2_2563 0.81 -0.4 3,022,303 - Pf6N2E2_2563 0.81 -0.7 3,022,303 - Pf6N2E2_2563 0.81 +1.5 3,022,303 - Pf6N2E2_2563 0.81 -1.7 3,022,303 - Pf6N2E2_2563 0.81 +0.9 3,022,303 - Pf6N2E2_2563 0.81 +1.3 3,022,303 - Pf6N2E2_2563 0.81 +0.8 3,022,303 - Pf6N2E2_2563 0.81 +1.0 3,022,304 + Pf6N2E2_2563 0.82 -0.7 3,022,304 + Pf6N2E2_2563 0.82 +1.3 3,022,304 + Pf6N2E2_2563 0.82 +0.8 3,022,304 + Pf6N2E2_2563 0.82 +1.0 3,022,305 - Pf6N2E2_2563 0.82 +0.7 3,022,305 - Pf6N2E2_2563 0.82 +0.4 3,022,305 - Pf6N2E2_2563 0.82 +1.3 3,022,305 - Pf6N2E2_2563 0.82 +0.7 3,022,305 - Pf6N2E2_2563 0.82 +1.7 3,022,306 + Pf6N2E2_2563 0.82 +0.9 3,022,306 + Pf6N2E2_2563 0.82 +1.5 3,022,306 + Pf6N2E2_2563 0.82 +0.5 3,022,306 + Pf6N2E2_2563 0.82 -0.4 3,022,307 - Pf6N2E2_2563 0.82 +0.3 3,022,307 - Pf6N2E2_2563 0.82 +0.8 3,022,307 - Pf6N2E2_2563 0.82 +1.5 3,022,307 - Pf6N2E2_2563 0.82 +0.5 3,022,363 - +0.9 3,022,363 - +0.8 3,022,363 - +0.3 3,022,384 - +0.7 3,022,384 - -1.3 3,022,384 - +1.0 3,022,539 - Pf6N2E2_2564 0.33 -3.1 3,022,706 + Pf6N2E2_2564 0.67 -0.5 3,022,707 - Pf6N2E2_2564 0.68 -0.8 3,022,707 - Pf6N2E2_2564 0.68 -0.9 3,022,741 - Pf6N2E2_2564 0.75 +0.1 3,022,742 + Pf6N2E2_2564 0.75 -1.5 3,022,742 + Pf6N2E2_2564 0.75 +0.2 3,022,781 + Pf6N2E2_2564 0.83 -1.9 3,022,781 + Pf6N2E2_2564 0.83 -1.1 3,022,782 - Pf6N2E2_2564 0.83 -1.5 3,022,782 - Pf6N2E2_2564 0.83 -1.3 3,022,854 + -0.1 3,022,854 + +0.1 3,022,863 + -1.4 3,022,864 - -0.1 3,022,900 + -0.9 3,022,900 + -1.8 3,022,900 + -0.4 3,022,900 + -0.5 3,022,900 + -1.1 3,022,900 + -2.2 3,022,901 - +0.4 3,022,901 - -1.1 3,022,901 - -0.1 3,022,937 + -0.3 3,022,937 + -0.8 3,023,037 + Pf6N2E2_2565 0.11 +0.0 3,023,037 + Pf6N2E2_2565 0.11 -0.5 3,023,037 + Pf6N2E2_2565 0.11 -1.3 3,023,037 + Pf6N2E2_2565 0.11 +0.9 3,023,037 + Pf6N2E2_2565 0.11 -0.2 3,023,037 + Pf6N2E2_2565 0.11 +0.2 3,023,037 + Pf6N2E2_2565 0.11 +0.1 3,023,037 + Pf6N2E2_2565 0.11 -1.4 3,023,037 + Pf6N2E2_2565 0.11 -1.2 3,023,037 + Pf6N2E2_2565 0.11 -0.8 3,023,037 + Pf6N2E2_2565 0.11 +0.1 3,023,037 + Pf6N2E2_2565 0.11 -0.4 3,023,038 - Pf6N2E2_2565 0.11 -0.1 3,023,038 - Pf6N2E2_2565 0.11 -1.2 3,023,038 - Pf6N2E2_2565 0.11 -1.0 3,023,038 - Pf6N2E2_2565 0.11 +0.5 3,023,038 - Pf6N2E2_2565 0.11 +0.0 3,023,038 - Pf6N2E2_2565 0.11 +0.0 3,023,038 - Pf6N2E2_2565 0.11 +0.4 3,023,038 - Pf6N2E2_2565 0.11 +0.1 3,023,038 - Pf6N2E2_2565 0.11 -1.7 3,023,052 + Pf6N2E2_2565 0.12 -0.4 3,023,157 - Pf6N2E2_2565 0.24 +0.3 3,023,177 + Pf6N2E2_2565 0.26 -0.6 3,023,177 + Pf6N2E2_2565 0.26 -0.3 3,023,178 - Pf6N2E2_2565 0.26 -0.2 3,023,178 - Pf6N2E2_2565 0.26 -0.8 3,023,606 - Pf6N2E2_2565 0.71 +2.5 3,023,760 - Pf6N2E2_2565 0.88 +0.1 3,023,760 - Pf6N2E2_2565 0.88 +0.0 3,023,798 - -0.1 3,023,811 + -2.0
Or see this region's nucleotide sequence