Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2450 and Pf6N2E2_2451 are separated by 54 nucleotides Pf6N2E2_2451 and Pf6N2E2_2452 are separated by 500 nucleotides Pf6N2E2_2452 and Pf6N2E2_2453 are separated by 139 nucleotides
Pf6N2E2_2450 - DEAD-box ATP-dependent RNA helicase CshA (EC 3.6.4.13), at 2,901,123 to 2,902,796
_2450
Pf6N2E2_2451 - hypothetical protein, at 2,902,851 to 2,903,003
_2451
Pf6N2E2_2452 - Spermidine synthase (EC 2.5.1.16), at 2,903,504 to 2,904,259
_2452
Pf6N2E2_2453 - 2-keto-3-deoxy-D-arabino-heptulosonate-7- phosphate synthase II (EC 2.5.1.54), at 2,904,399 to 2,905,748
_2453
Position (kb)
2903
2904
2905 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2902.809 kb on - strand at 2903.019 kb on - strand at 2903.062 kb on + strand at 2903.191 kb on - strand at 2903.482 kb on - strand at 2903.679 kb on + strand, within Pf6N2E2_2452 at 2903.680 kb on - strand, within Pf6N2E2_2452 at 2903.680 kb on - strand, within Pf6N2E2_2452 at 2903.827 kb on + strand, within Pf6N2E2_2452 at 2903.830 kb on - strand, within Pf6N2E2_2452 at 2903.901 kb on + strand, within Pf6N2E2_2452 at 2903.901 kb on + strand, within Pf6N2E2_2452 at 2903.901 kb on + strand, within Pf6N2E2_2452 at 2903.902 kb on - strand, within Pf6N2E2_2452 at 2903.902 kb on - strand, within Pf6N2E2_2452 at 2903.902 kb on - strand, within Pf6N2E2_2452 at 2903.931 kb on + strand, within Pf6N2E2_2452 at 2904.022 kb on + strand, within Pf6N2E2_2452 at 2904.023 kb on - strand, within Pf6N2E2_2452 at 2904.023 kb on - strand, within Pf6N2E2_2452 at 2904.023 kb on - strand, within Pf6N2E2_2452 at 2904.097 kb on + strand, within Pf6N2E2_2452 at 2904.099 kb on + strand, within Pf6N2E2_2452 at 2904.099 kb on + strand, within Pf6N2E2_2452 at 2904.099 kb on + strand, within Pf6N2E2_2452 at 2904.099 kb on + strand, within Pf6N2E2_2452 at 2904.099 kb on + strand, within Pf6N2E2_2452 at 2904.099 kb on + strand, within Pf6N2E2_2452 at 2904.100 kb on - strand, within Pf6N2E2_2452 at 2904.100 kb on - strand, within Pf6N2E2_2452 at 2904.100 kb on - strand, within Pf6N2E2_2452 at 2904.100 kb on - strand, within Pf6N2E2_2452 at 2904.100 kb on - strand, within Pf6N2E2_2452 at 2904.106 kb on - strand, within Pf6N2E2_2452 at 2904.352 kb on + strand at 2904.353 kb on - strand at 2904.363 kb on + strand at 2904.391 kb on + strand at 2904.392 kb on - strand at 2904.874 kb on - strand, within Pf6N2E2_2453 at 2904.874 kb on - strand, within Pf6N2E2_2453 at 2904.996 kb on + strand, within Pf6N2E2_2453 at 2905.119 kb on + strand, within Pf6N2E2_2453 at 2905.120 kb on - strand, within Pf6N2E2_2453 at 2905.162 kb on - strand, within Pf6N2E2_2453 at 2905.164 kb on + strand, within Pf6N2E2_2453 at 2905.164 kb on + strand, within Pf6N2E2_2453 at 2905.164 kb on + strand, within Pf6N2E2_2453 at 2905.165 kb on - strand, within Pf6N2E2_2453
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 2,902,809 - +1.0 2,903,019 - +1.5 2,903,062 + -0.3 2,903,191 - +0.1 2,903,482 - -0.1 2,903,679 + Pf6N2E2_2452 0.23 +1.5 2,903,680 - Pf6N2E2_2452 0.23 +0.2 2,903,680 - Pf6N2E2_2452 0.23 -0.1 2,903,827 + Pf6N2E2_2452 0.43 -0.9 2,903,830 - Pf6N2E2_2452 0.43 -0.5 2,903,901 + Pf6N2E2_2452 0.53 +0.3 2,903,901 + Pf6N2E2_2452 0.53 -0.4 2,903,901 + Pf6N2E2_2452 0.53 +1.5 2,903,902 - Pf6N2E2_2452 0.53 -1.1 2,903,902 - Pf6N2E2_2452 0.53 +1.5 2,903,902 - Pf6N2E2_2452 0.53 +1.1 2,903,931 + Pf6N2E2_2452 0.56 +0.6 2,904,022 + Pf6N2E2_2452 0.69 +0.2 2,904,023 - Pf6N2E2_2452 0.69 +0.3 2,904,023 - Pf6N2E2_2452 0.69 -1.3 2,904,023 - Pf6N2E2_2452 0.69 +0.9 2,904,097 + Pf6N2E2_2452 0.78 -0.1 2,904,099 + Pf6N2E2_2452 0.79 +1.1 2,904,099 + Pf6N2E2_2452 0.79 +1.5 2,904,099 + Pf6N2E2_2452 0.79 +1.1 2,904,099 + Pf6N2E2_2452 0.79 -0.6 2,904,099 + Pf6N2E2_2452 0.79 -0.3 2,904,099 + Pf6N2E2_2452 0.79 +0.2 2,904,100 - Pf6N2E2_2452 0.79 -0.1 2,904,100 - Pf6N2E2_2452 0.79 +0.2 2,904,100 - Pf6N2E2_2452 0.79 -0.3 2,904,100 - Pf6N2E2_2452 0.79 -1.0 2,904,100 - Pf6N2E2_2452 0.79 +0.1 2,904,106 - Pf6N2E2_2452 0.80 -0.3 2,904,352 + -0.1 2,904,353 - +0.0 2,904,363 + -0.3 2,904,391 + +0.7 2,904,392 - +1.5 2,904,874 - Pf6N2E2_2453 0.35 -0.2 2,904,874 - Pf6N2E2_2453 0.35 -0.1 2,904,996 + Pf6N2E2_2453 0.44 +0.4 2,905,119 + Pf6N2E2_2453 0.53 -3.0 2,905,120 - Pf6N2E2_2453 0.53 +0.0 2,905,162 - Pf6N2E2_2453 0.57 -0.1 2,905,164 + Pf6N2E2_2453 0.57 -0.6 2,905,164 + Pf6N2E2_2453 0.57 -0.5 2,905,164 + Pf6N2E2_2453 0.57 -0.1 2,905,165 - Pf6N2E2_2453 0.57 +0.1
Or see this region's nucleotide sequence