Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2389

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
2,826,447	+		Pf6N2E2_2388	0.47	+0.5
2,826,447	+		Pf6N2E2_2388	0.47	-1.3
2,826,447	+		Pf6N2E2_2388	0.47	+0.5
2,826,451	+		Pf6N2E2_2388	0.47	-1.1
2,826,452	-		Pf6N2E2_2388	0.48	+2.5
2,826,507	+		Pf6N2E2_2388	0.53	+0.3
2,826,726	+		Pf6N2E2_2388	0.75	-0.1
2,826,970	+				-1.4
2,826,970	+				+0.8
2,826,971	-				+1.5
2,827,016	+				+1.5
2,827,040	+				-0.1
2,827,040	+				-0.1
2,827,041	-				-1.7
2,827,069	-				+0.1
2,827,069	-				-0.8
2,827,069	-				-0.1
2,827,069	-				-0.4
2,827,112	-				+0.5
2,827,112	-				+0.5
2,827,189	-				-2.1
2,827,189	-				-0.1
2,827,189	-				-2.5
2,827,196	-				+0.5
2,827,196	-				-0.5
2,827,196	-				-0.9
2,827,196	-				+0.0
2,827,196	-				-2.3
2,827,196	-				-0.9
2,827,198	+				+0.5
2,827,199	-				-0.9
2,827,199	-				-0.4
2,827,199	-				-1.1
2,827,199	-				-1.3
2,827,368	+				-0.1
2,827,368	+				+0.5
2,827,645	+		Pf6N2E2_2389	0.18	+1.5
2,827,646	-		Pf6N2E2_2389	0.18	-3.3
2,827,770	+		Pf6N2E2_2389	0.23	+0.9
2,827,771	-		Pf6N2E2_2389	0.23	-1.5
2,827,822	-		Pf6N2E2_2389	0.25	-2.7
2,827,849	-		Pf6N2E2_2389	0.26	-3.3
2,827,849	-		Pf6N2E2_2389	0.26	-1.9
2,828,019	+		Pf6N2E2_2389	0.34	+1.5
2,828,020	-		Pf6N2E2_2389	0.34	-2.6
2,828,435	+		Pf6N2E2_2389	0.51	-1.5
2,828,435	+		Pf6N2E2_2389	0.51	+1.5
2,828,435	+		Pf6N2E2_2389	0.51	-0.1
2,828,436	-		Pf6N2E2_2389	0.51	-1.3
2,828,487	-		Pf6N2E2_2389	0.53	-3.1
2,828,559	+		Pf6N2E2_2389	0.56	-0.4
2,828,559	+		Pf6N2E2_2389	0.56	+1.5
2,828,560	-		Pf6N2E2_2389	0.56	-1.5
2,828,560	-		Pf6N2E2_2389	0.56	-0.8
2,828,622	+		Pf6N2E2_2389	0.59	-1.3
2,828,622	+		Pf6N2E2_2389	0.59	-1.5
2,828,622	+		Pf6N2E2_2389	0.59	-1.7
2,828,622	+		Pf6N2E2_2389	0.59	+1.5
2,828,622	+		Pf6N2E2_2389	0.59	-2.5
2,828,623	-		Pf6N2E2_2389	0.59	-2.3
2,828,623	-		Pf6N2E2_2389	0.59	-1.9
2,828,623	-		Pf6N2E2_2389	0.59	-2.3
2,828,676	+		Pf6N2E2_2389	0.61	-4.0
2,828,676	+		Pf6N2E2_2389	0.61	-1.2
2,828,676	+		Pf6N2E2_2389	0.61	+0.5
2,828,677	-		Pf6N2E2_2389	0.61	-2.5
2,828,677	-		Pf6N2E2_2389	0.61	-1.5
2,828,677	-		Pf6N2E2_2389	0.61	+0.5
2,828,677	-		Pf6N2E2_2389	0.61	-0.8
2,828,677	-		Pf6N2E2_2389	0.61	+0.5
2,828,808	+		Pf6N2E2_2389	0.67	-1.9
2,828,808	+		Pf6N2E2_2389	0.67	-3.0
2,828,808	+		Pf6N2E2_2389	0.67	-3.0
2,828,809	-		Pf6N2E2_2389	0.67	-3.3
2,828,809	-		Pf6N2E2_2389	0.67	-2.4
2,828,809	-		Pf6N2E2_2389	0.67	-0.7
2,828,889	+		Pf6N2E2_2389	0.70	-0.1
2,828,889	+		Pf6N2E2_2389	0.70	+1.5
2,828,889	+		Pf6N2E2_2389	0.70	-1.7
2,828,890	-		Pf6N2E2_2389	0.70	-2.2
2,828,890	-		Pf6N2E2_2389	0.70	-2.6
2,828,890	-		Pf6N2E2_2389	0.70	-2.3
2,828,941	-		Pf6N2E2_2389	0.72	-2.2
2,829,486	-				-0.5
2,829,486	-				-1.8
2,829,505	+				-0.8
2,829,506	-				-2.9
2,829,551	+				+0.5
2,829,551	+				-1.7
2,829,551	+				-0.1
2,829,552	-				-1.7
2,829,552	-				-1.4
2,829,552	-				-2.1
2,829,552	-				-1.6
2,829,552	-				-0.9
2,829,584	+				+2.5
2,829,585	-				-2.1
2,829,585	-				-1.1
2,829,585	-				-1.3
2,829,589	-				+1.5
2,829,589	-				-1.8
2,829,589	-				+0.5
2,829,589	-				-2.4
2,829,593	+				-1.7
2,829,594	-				-1.4
2,829,613	+		Pf6N2E2_2390	0.27	-0.5
2,829,614	-		Pf6N2E2_2390	0.28	-0.6
2,829,690	+		Pf6N2E2_2390	0.64	-1.2
2,829,731	+		Pf6N2E2_2390	0.84	-2.3
2,829,761	+				+1.5
2,829,761	+				-1.4
2,829,762	-				-1.2
2,829,762	-				-0.2
2,829,824	+				+1.5
2,829,873	-		Pf6N2E2_2391	0.11	-0.5
2,829,889	+		Pf6N2E2_2391	0.13	-0.1
2,829,897	+		Pf6N2E2_2391	0.14	-0.1
2,829,897	+		Pf6N2E2_2391	0.14	-0.1
2,829,931	+		Pf6N2E2_2391	0.18	-0.8
2,829,932	-		Pf6N2E2_2391	0.18	-2.5
2,829,932	-		Pf6N2E2_2391	0.18	-0.3
2,829,932	-		Pf6N2E2_2391	0.18	-1.7
2,829,944	+		Pf6N2E2_2391	0.20	-1.3
2,829,945	-		Pf6N2E2_2391	0.20	-1.3
2,829,977	+		Pf6N2E2_2391	0.24	+0.5
2,829,978	-		Pf6N2E2_2391	0.24	-0.8
2,830,101	+		Pf6N2E2_2391	0.39	-0.7
2,830,143	+		Pf6N2E2_2391	0.44	-0.9
2,830,143	+		Pf6N2E2_2391	0.44	+0.5
2,830,143	+		Pf6N2E2_2391	0.44	+0.8
2,830,143	+		Pf6N2E2_2391	0.44	+0.8
2,830,143	+		Pf6N2E2_2391	0.44	-0.5
2,830,143	+		Pf6N2E2_2391	0.44	+0.1
2,830,144	-		Pf6N2E2_2391	0.44	-2.2
2,830,144	-		Pf6N2E2_2391	0.44	-0.5
2,830,144	-		Pf6N2E2_2391	0.44	-0.8
2,830,144	-				+1.5
2,830,206	+		Pf6N2E2_2391	0.52	-0.3
2,830,206	+		Pf6N2E2_2391	0.52	-0.7
2,830,209	+		Pf6N2E2_2391	0.52	+0.5
2,830,210	-		Pf6N2E2_2391	0.52	-1.0
2,830,210	-		Pf6N2E2_2391	0.52	+0.1
2,830,210	-		Pf6N2E2_2391	0.52	-0.6
2,830,231	+		Pf6N2E2_2391	0.55	-0.3
2,830,232	-		Pf6N2E2_2391	0.55	-0.5
2,830,232	-		Pf6N2E2_2391	0.55	-2.6
2,830,253	+		Pf6N2E2_2391	0.57	-1.8
2,830,272	+		Pf6N2E2_2391	0.60	-0.1
2,830,272	+		Pf6N2E2_2391	0.60	-0.4
2,830,273	-		Pf6N2E2_2391	0.60	-1.8
2,830,308	+		Pf6N2E2_2391	0.64	-0.6
2,830,308	+		Pf6N2E2_2391	0.64	+0.7
2,830,309	-		Pf6N2E2_2391	0.64	+0.3
2,830,455	+		Pf6N2E2_2391	0.82	+0.5
2,830,455	+		Pf6N2E2_2391	0.82	-0.5
2,830,455	+		Pf6N2E2_2391	0.82	+0.7
2,830,455	+		Pf6N2E2_2391	0.82	-1.1
2,830,455	+		Pf6N2E2_2391	0.82	+3.1
2,830,456	-		Pf6N2E2_2391	0.82	+1.5
2,830,512	-		Pf6N2E2_2391	0.89	+0.7
2,830,512	-		Pf6N2E2_2391	0.89	+1.5
2,830,512	-		Pf6N2E2_2391	0.89	+1.5