Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2378 and Pf6N2E2_2379 overlap by 4 nucleotides Pf6N2E2_2379 and Pf6N2E2_2380 overlap by 1 nucleotides Pf6N2E2_2380 and Pf6N2E2_2381 are separated by 10 nucleotides Pf6N2E2_2381 and Pf6N2E2_2382 are separated by 197 nucleotides
Pf6N2E2_2378 - Nitrite reductase (NAD(P)H) (EC 1.7.1.4) (from data) , at 2,814,933 to 2,817,386
_2378
Pf6N2E2_2379 - Nitrite reductase [NAD(P)H] small subunit (EC 1.7.1.4), at 2,817,383 to 2,817,700
_2379
Pf6N2E2_2380 - Nitrate reductase (EC 1.7.99.4) (from data) , at 2,817,700 to 2,820,417
_2380
Pf6N2E2_2381 - Uroporphyrinogen-III methyltransferase (EC 2.1.1.107), at 2,820,428 to 2,821,183
_2381
Pf6N2E2_2382 - Major porin and structural outer membrane porin OprF, at 2,821,381 to 2,822,415
_2382
Position (kb)
2817
2818
2819
2820
2821 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2816.713 kb on - strand, within Pf6N2E2_2378 at 2816.713 kb on - strand, within Pf6N2E2_2378 at 2816.713 kb on - strand, within Pf6N2E2_2378 at 2816.713 kb on - strand, within Pf6N2E2_2378 at 2816.719 kb on + strand, within Pf6N2E2_2378 at 2816.719 kb on + strand, within Pf6N2E2_2378 at 2816.720 kb on - strand, within Pf6N2E2_2378 at 2817.048 kb on + strand, within Pf6N2E2_2378 at 2817.048 kb on + strand, within Pf6N2E2_2378 at 2817.048 kb on + strand, within Pf6N2E2_2378 at 2817.048 kb on + strand, within Pf6N2E2_2378 at 2817.048 kb on + strand, within Pf6N2E2_2378 at 2817.048 kb on + strand, within Pf6N2E2_2378 at 2817.048 kb on + strand, within Pf6N2E2_2378 at 2817.048 kb on + strand, within Pf6N2E2_2378 at 2817.049 kb on - strand, within Pf6N2E2_2378 at 2817.049 kb on - strand, within Pf6N2E2_2378 at 2817.049 kb on - strand, within Pf6N2E2_2378 at 2817.049 kb on - strand, within Pf6N2E2_2378 at 2817.049 kb on - strand, within Pf6N2E2_2378 at 2817.049 kb on - strand, within Pf6N2E2_2378 at 2817.049 kb on - strand, within Pf6N2E2_2378 at 2817.130 kb on - strand, within Pf6N2E2_2378 at 2817.171 kb on + strand at 2817.171 kb on + strand at 2817.172 kb on - strand at 2817.172 kb on - strand at 2817.192 kb on + strand at 2817.192 kb on + strand at 2817.192 kb on + strand at 2817.192 kb on + strand at 2817.192 kb on + strand at 2817.192 kb on + strand at 2817.192 kb on + strand at 2817.193 kb on - strand at 2817.193 kb on - strand at 2817.193 kb on - strand at 2817.193 kb on - strand at 2817.398 kb on - strand at 2817.672 kb on + strand at 2818.012 kb on + strand, within Pf6N2E2_2380 at 2818.012 kb on + strand, within Pf6N2E2_2380 at 2818.012 kb on + strand, within Pf6N2E2_2380 at 2818.013 kb on - strand, within Pf6N2E2_2380 at 2818.013 kb on - strand, within Pf6N2E2_2380 at 2818.013 kb on - strand, within Pf6N2E2_2380 at 2818.042 kb on + strand, within Pf6N2E2_2380 at 2818.042 kb on + strand, within Pf6N2E2_2380 at 2818.042 kb on + strand, within Pf6N2E2_2380 at 2818.043 kb on - strand, within Pf6N2E2_2380 at 2818.043 kb on - strand, within Pf6N2E2_2380 at 2818.043 kb on - strand, within Pf6N2E2_2380 at 2818.043 kb on - strand, within Pf6N2E2_2380 at 2818.043 kb on - strand, within Pf6N2E2_2380 at 2818.043 kb on - strand, within Pf6N2E2_2380 at 2818.043 kb on - strand, within Pf6N2E2_2380 at 2818.043 kb on - strand, within Pf6N2E2_2380 at 2818.043 kb on - strand, within Pf6N2E2_2380 at 2818.043 kb on - strand, within Pf6N2E2_2380 at 2818.043 kb on - strand, within Pf6N2E2_2380 at 2818.045 kb on + strand, within Pf6N2E2_2380 at 2818.045 kb on + strand, within Pf6N2E2_2380 at 2818.045 kb on + strand, within Pf6N2E2_2380 at 2818.046 kb on - strand, within Pf6N2E2_2380 at 2818.046 kb on - strand, within Pf6N2E2_2380 at 2818.046 kb on - strand, within Pf6N2E2_2380 at 2818.046 kb on - strand, within Pf6N2E2_2380 at 2818.046 kb on - strand, within Pf6N2E2_2380 at 2818.046 kb on - strand, within Pf6N2E2_2380 at 2818.046 kb on - strand, within Pf6N2E2_2380 at 2818.086 kb on + strand, within Pf6N2E2_2380 at 2818.136 kb on - strand, within Pf6N2E2_2380 at 2818.136 kb on - strand, within Pf6N2E2_2380 at 2818.483 kb on + strand, within Pf6N2E2_2380 at 2818.643 kb on + strand, within Pf6N2E2_2380 at 2818.643 kb on + strand, within Pf6N2E2_2380 at 2818.643 kb on + strand, within Pf6N2E2_2380 at 2818.643 kb on + strand, within Pf6N2E2_2380 at 2818.643 kb on + strand, within Pf6N2E2_2380 at 2818.643 kb on + strand, within Pf6N2E2_2380 at 2818.643 kb on + strand, within Pf6N2E2_2380 at 2818.643 kb on + strand, within Pf6N2E2_2380 at 2818.644 kb on - strand, within Pf6N2E2_2380 at 2818.644 kb on - strand, within Pf6N2E2_2380 at 2818.644 kb on - strand, within Pf6N2E2_2380 at 2818.644 kb on - strand, within Pf6N2E2_2380 at 2818.644 kb on - strand, within Pf6N2E2_2380 at 2819.239 kb on + strand, within Pf6N2E2_2380 at 2819.239 kb on + strand, within Pf6N2E2_2380 at 2819.239 kb on + strand, within Pf6N2E2_2380 at 2819.239 kb on + strand, within Pf6N2E2_2380 at 2819.362 kb on + strand, within Pf6N2E2_2380 at 2819.362 kb on + strand, within Pf6N2E2_2380 at 2819.362 kb on + strand, within Pf6N2E2_2380 at 2819.363 kb on - strand, within Pf6N2E2_2380 at 2819.363 kb on - strand, within Pf6N2E2_2380 at 2819.363 kb on - strand, within Pf6N2E2_2380 at 2819.730 kb on + strand, within Pf6N2E2_2380 at 2819.896 kb on + strand, within Pf6N2E2_2380 at 2819.896 kb on + strand, within Pf6N2E2_2380 at 2820.031 kb on + strand, within Pf6N2E2_2380 at 2820.072 kb on - strand, within Pf6N2E2_2380 at 2820.288 kb on + strand at 2820.288 kb on + strand at 2820.427 kb on + strand at 2820.428 kb on - strand at 2820.478 kb on - strand at 2820.478 kb on - strand at 2820.926 kb on + strand, within Pf6N2E2_2381 at 2820.926 kb on + strand, within Pf6N2E2_2381 at 2820.926 kb on + strand, within Pf6N2E2_2381 at 2820.926 kb on + strand, within Pf6N2E2_2381 at 2820.926 kb on + strand, within Pf6N2E2_2381 at 2820.926 kb on + strand, within Pf6N2E2_2381 at 2820.926 kb on + strand, within Pf6N2E2_2381 at 2820.927 kb on - strand, within Pf6N2E2_2381 at 2820.927 kb on - strand, within Pf6N2E2_2381 at 2820.927 kb on - strand, within Pf6N2E2_2381 at 2820.927 kb on - strand, within Pf6N2E2_2381 at 2820.927 kb on - strand, within Pf6N2E2_2381 at 2820.927 kb on - strand, within Pf6N2E2_2381 at 2820.927 kb on - strand, within Pf6N2E2_2381 at 2821.179 kb on + strand at 2821.179 kb on + strand at 2821.179 kb on + strand at 2821.180 kb on - strand at 2821.291 kb on + strand at 2821.291 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 2,816,713 - Pf6N2E2_2378 0.73 +0.9 2,816,713 - Pf6N2E2_2378 0.73 -0.1 2,816,713 - Pf6N2E2_2378 0.73 +0.5 2,816,713 - Pf6N2E2_2378 0.73 -0.2 2,816,719 + Pf6N2E2_2378 0.73 +0.9 2,816,719 + Pf6N2E2_2378 0.73 -0.3 2,816,720 - Pf6N2E2_2378 0.73 -0.4 2,817,048 + Pf6N2E2_2378 0.86 -0.2 2,817,048 + Pf6N2E2_2378 0.86 -0.5 2,817,048 + Pf6N2E2_2378 0.86 -0.9 2,817,048 + Pf6N2E2_2378 0.86 +0.1 2,817,048 + Pf6N2E2_2378 0.86 -0.7 2,817,048 + Pf6N2E2_2378 0.86 -0.7 2,817,048 + Pf6N2E2_2378 0.86 +0.4 2,817,048 + Pf6N2E2_2378 0.86 -0.5 2,817,049 - Pf6N2E2_2378 0.86 -0.4 2,817,049 - Pf6N2E2_2378 0.86 -0.7 2,817,049 - Pf6N2E2_2378 0.86 -0.1 2,817,049 - Pf6N2E2_2378 0.86 +0.1 2,817,049 - Pf6N2E2_2378 0.86 -0.0 2,817,049 - Pf6N2E2_2378 0.86 +0.9 2,817,049 - Pf6N2E2_2378 0.86 -0.1 2,817,130 - Pf6N2E2_2378 0.90 +0.3 2,817,171 + -1.1 2,817,171 + -0.4 2,817,172 - +2.1 2,817,172 - -0.1 2,817,192 + -0.5 2,817,192 + +0.5 2,817,192 + +1.5 2,817,192 + +0.4 2,817,192 + +0.1 2,817,192 + +0.8 2,817,192 + +0.3 2,817,193 - -0.4 2,817,193 - -1.1 2,817,193 - -0.3 2,817,193 - -0.1 2,817,398 - +0.1 2,817,672 + +0.2 2,818,012 + Pf6N2E2_2380 0.11 -0.1 2,818,012 + Pf6N2E2_2380 0.11 +0.4 2,818,012 + Pf6N2E2_2380 0.11 +1.3 2,818,013 - Pf6N2E2_2380 0.12 +0.5 2,818,013 - Pf6N2E2_2380 0.12 -0.4 2,818,013 - Pf6N2E2_2380 0.12 -0.5 2,818,042 + Pf6N2E2_2380 0.13 -0.1 2,818,042 + Pf6N2E2_2380 0.13 +0.7 2,818,042 + Pf6N2E2_2380 0.13 -0.4 2,818,043 - Pf6N2E2_2380 0.13 -0.2 2,818,043 - Pf6N2E2_2380 0.13 -0.7 2,818,043 - Pf6N2E2_2380 0.13 +0.5 2,818,043 - Pf6N2E2_2380 0.13 -1.3 2,818,043 - Pf6N2E2_2380 0.13 -1.4 2,818,043 - Pf6N2E2_2380 0.13 +0.6 2,818,043 - Pf6N2E2_2380 0.13 -2.0 2,818,043 - Pf6N2E2_2380 0.13 +0.8 2,818,043 - Pf6N2E2_2380 0.13 +0.2 2,818,043 - Pf6N2E2_2380 0.13 -1.1 2,818,043 - Pf6N2E2_2380 0.13 -0.1 2,818,045 + Pf6N2E2_2380 0.13 -0.7 2,818,045 + Pf6N2E2_2380 0.13 +0.2 2,818,045 + Pf6N2E2_2380 0.13 +0.6 2,818,046 - Pf6N2E2_2380 0.13 -1.8 2,818,046 - Pf6N2E2_2380 0.13 +0.1 2,818,046 - Pf6N2E2_2380 0.13 +0.5 2,818,046 - Pf6N2E2_2380 0.13 -1.1 2,818,046 - Pf6N2E2_2380 0.13 +1.5 2,818,046 - Pf6N2E2_2380 0.13 -0.2 2,818,046 - Pf6N2E2_2380 0.13 -0.9 2,818,086 + Pf6N2E2_2380 0.14 +0.9 2,818,136 - Pf6N2E2_2380 0.16 +1.5 2,818,136 - Pf6N2E2_2380 0.16 +0.2 2,818,483 + Pf6N2E2_2380 0.29 -0.1 2,818,643 + Pf6N2E2_2380 0.35 -1.1 2,818,643 + Pf6N2E2_2380 0.35 -0.2 2,818,643 + Pf6N2E2_2380 0.35 -0.7 2,818,643 + Pf6N2E2_2380 0.35 +0.7 2,818,643 + Pf6N2E2_2380 0.35 -0.1 2,818,643 + Pf6N2E2_2380 0.35 -0.4 2,818,643 + Pf6N2E2_2380 0.35 +0.1 2,818,643 + Pf6N2E2_2380 0.35 -0.2 2,818,644 - Pf6N2E2_2380 0.35 -0.1 2,818,644 - Pf6N2E2_2380 0.35 +1.5 2,818,644 - Pf6N2E2_2380 0.35 -0.5 2,818,644 - Pf6N2E2_2380 0.35 +2.5 2,818,644 - Pf6N2E2_2380 0.35 -0.8 2,819,239 + Pf6N2E2_2380 0.57 -0.4 2,819,239 + Pf6N2E2_2380 0.57 +1.7 2,819,239 + Pf6N2E2_2380 0.57 -1.5 2,819,239 + Pf6N2E2_2380 0.57 -0.9 2,819,362 + Pf6N2E2_2380 0.61 -0.9 2,819,362 + Pf6N2E2_2380 0.61 -2.1 2,819,362 + Pf6N2E2_2380 0.61 -0.2 2,819,363 - Pf6N2E2_2380 0.61 -1.5 2,819,363 - Pf6N2E2_2380 0.61 -0.1 2,819,363 - Pf6N2E2_2380 0.61 -0.1 2,819,730 + Pf6N2E2_2380 0.75 +1.2 2,819,896 + Pf6N2E2_2380 0.81 -0.4 2,819,896 + Pf6N2E2_2380 0.81 -0.8 2,820,031 + Pf6N2E2_2380 0.86 +0.5 2,820,072 - Pf6N2E2_2380 0.87 +0.1 2,820,288 + -2.1 2,820,288 + +1.5 2,820,427 + -1.7 2,820,428 - -1.8 2,820,478 - +2.5 2,820,478 - +1.5 2,820,926 + Pf6N2E2_2381 0.66 -1.3 2,820,926 + Pf6N2E2_2381 0.66 +1.5 2,820,926 + Pf6N2E2_2381 0.66 +0.5 2,820,926 + Pf6N2E2_2381 0.66 -1.8 2,820,926 + Pf6N2E2_2381 0.66 -0.3 2,820,926 + Pf6N2E2_2381 0.66 +0.1 2,820,926 + Pf6N2E2_2381 0.66 -0.3 2,820,927 - Pf6N2E2_2381 0.66 +0.5 2,820,927 - Pf6N2E2_2381 0.66 -0.5 2,820,927 - Pf6N2E2_2381 0.66 +1.5 2,820,927 - Pf6N2E2_2381 0.66 +0.9 2,820,927 - Pf6N2E2_2381 0.66 +1.5 2,820,927 - Pf6N2E2_2381 0.66 -1.7 2,820,927 - Pf6N2E2_2381 0.66 +1.5 2,821,179 + -1.4 2,821,179 + -0.8 2,821,179 + -0.6 2,821,180 - +1.5 2,821,291 + -0.2 2,821,291 + +0.5
Or see this region's nucleotide sequence