Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2377 and Pf6N2E2_2378 are separated by 455 nucleotides Pf6N2E2_2378 and Pf6N2E2_2379 overlap by 4 nucleotides Pf6N2E2_2379 and Pf6N2E2_2380 overlap by 1 nucleotides
Pf6N2E2_2377 - Serine/threonine protein kinase (EC 2.7.11.1), at 2,812,804 to 2,814,477
_2377
Pf6N2E2_2378 - Nitrite reductase (NAD(P)H) (EC 1.7.1.4) (from data) , at 2,814,933 to 2,817,386
_2378
Pf6N2E2_2379 - Nitrite reductase [NAD(P)H] small subunit (EC 1.7.1.4), at 2,817,383 to 2,817,700
_2379
Pf6N2E2_2380 - Nitrate reductase (EC 1.7.99.4) (from data) , at 2,817,700 to 2,820,417
_2380
Position (kb)
2814
2815
2816
2817
2818 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2813.938 kb on + strand, within Pf6N2E2_2377 at 2813.938 kb on + strand, within Pf6N2E2_2377 at 2813.939 kb on - strand, within Pf6N2E2_2377 at 2814.016 kb on + strand, within Pf6N2E2_2377 at 2814.070 kb on + strand, within Pf6N2E2_2377 at 2814.070 kb on + strand, within Pf6N2E2_2377 at 2814.070 kb on + strand, within Pf6N2E2_2377 at 2814.070 kb on + strand, within Pf6N2E2_2377 at 2814.071 kb on - strand, within Pf6N2E2_2377 at 2814.071 kb on - strand, within Pf6N2E2_2377 at 2814.071 kb on - strand, within Pf6N2E2_2377 at 2814.071 kb on - strand, within Pf6N2E2_2377 at 2814.071 kb on - strand, within Pf6N2E2_2377 at 2814.071 kb on - strand, within Pf6N2E2_2377 at 2814.071 kb on - strand, within Pf6N2E2_2377 at 2814.071 kb on - strand, within Pf6N2E2_2377 at 2814.124 kb on + strand, within Pf6N2E2_2377 at 2814.125 kb on - strand, within Pf6N2E2_2377 at 2814.145 kb on + strand, within Pf6N2E2_2377 at 2814.145 kb on + strand, within Pf6N2E2_2377 at 2814.145 kb on + strand, within Pf6N2E2_2377 at 2814.146 kb on - strand, within Pf6N2E2_2377 at 2814.146 kb on - strand, within Pf6N2E2_2377 at 2814.146 kb on - strand, within Pf6N2E2_2377 at 2814.365 kb on + strand at 2814.365 kb on + strand at 2814.480 kb on - strand at 2814.497 kb on + strand at 2814.620 kb on + strand at 2814.668 kb on + strand at 2814.669 kb on - strand at 2814.669 kb on - strand at 2814.762 kb on + strand at 2814.762 kb on + strand at 2814.763 kb on - strand at 2814.763 kb on - strand at 2814.763 kb on - strand at 2814.763 kb on - strand at 2814.766 kb on + strand at 2814.766 kb on + strand at 2814.766 kb on + strand at 2814.766 kb on + strand at 2814.766 kb on + strand at 2814.767 kb on - strand at 2814.767 kb on - strand at 2814.767 kb on - strand at 2814.767 kb on - strand at 2814.767 kb on - strand at 2814.767 kb on - strand at 2815.020 kb on + strand at 2815.021 kb on - strand at 2815.059 kb on + strand at 2815.059 kb on + strand at 2815.059 kb on + strand at 2815.060 kb on - strand at 2815.060 kb on - strand at 2815.060 kb on - strand at 2815.137 kb on + strand at 2815.137 kb on + strand at 2815.236 kb on + strand, within Pf6N2E2_2378 at 2815.236 kb on + strand, within Pf6N2E2_2378 at 2815.741 kb on - strand, within Pf6N2E2_2378 at 2815.741 kb on - strand, within Pf6N2E2_2378 at 2815.755 kb on + strand, within Pf6N2E2_2378 at 2815.755 kb on + strand, within Pf6N2E2_2378 at 2815.755 kb on + strand, within Pf6N2E2_2378 at 2815.755 kb on + strand, within Pf6N2E2_2378 at 2815.756 kb on - strand, within Pf6N2E2_2378 at 2815.756 kb on - strand, within Pf6N2E2_2378 at 2815.869 kb on + strand, within Pf6N2E2_2378 at 2815.869 kb on + strand, within Pf6N2E2_2378 at 2815.869 kb on + strand, within Pf6N2E2_2378 at 2815.870 kb on - strand, within Pf6N2E2_2378 at 2815.870 kb on - strand, within Pf6N2E2_2378 at 2815.870 kb on - strand, within Pf6N2E2_2378 at 2815.870 kb on - strand, within Pf6N2E2_2378 at 2815.992 kb on + strand, within Pf6N2E2_2378 at 2816.067 kb on + strand, within Pf6N2E2_2378 at 2816.067 kb on + strand, within Pf6N2E2_2378 at 2816.067 kb on + strand, within Pf6N2E2_2378 at 2816.329 kb on + strand, within Pf6N2E2_2378 at 2816.330 kb on - strand, within Pf6N2E2_2378 at 2816.481 kb on + strand, within Pf6N2E2_2378 at 2816.535 kb on + strand, within Pf6N2E2_2378 at 2816.535 kb on + strand, within Pf6N2E2_2378 at 2816.535 kb on + strand, within Pf6N2E2_2378 at 2816.536 kb on - strand, within Pf6N2E2_2378 at 2816.536 kb on - strand, within Pf6N2E2_2378 at 2816.536 kb on - strand, within Pf6N2E2_2378 at 2816.536 kb on - strand, within Pf6N2E2_2378 at 2816.536 kb on - strand, within Pf6N2E2_2378 at 2816.538 kb on + strand, within Pf6N2E2_2378 at 2816.539 kb on - strand, within Pf6N2E2_2378 at 2816.539 kb on - strand, within Pf6N2E2_2378 at 2816.635 kb on - strand, within Pf6N2E2_2378 at 2816.713 kb on - strand, within Pf6N2E2_2378 at 2816.713 kb on - strand, within Pf6N2E2_2378 at 2816.713 kb on - strand, within Pf6N2E2_2378 at 2816.713 kb on - strand, within Pf6N2E2_2378 at 2816.719 kb on + strand, within Pf6N2E2_2378 at 2816.719 kb on + strand, within Pf6N2E2_2378 at 2816.720 kb on - strand, within Pf6N2E2_2378 at 2817.048 kb on + strand, within Pf6N2E2_2378 at 2817.048 kb on + strand, within Pf6N2E2_2378 at 2817.048 kb on + strand, within Pf6N2E2_2378 at 2817.048 kb on + strand, within Pf6N2E2_2378 at 2817.048 kb on + strand, within Pf6N2E2_2378 at 2817.048 kb on + strand, within Pf6N2E2_2378 at 2817.048 kb on + strand, within Pf6N2E2_2378 at 2817.048 kb on + strand, within Pf6N2E2_2378 at 2817.049 kb on - strand, within Pf6N2E2_2378 at 2817.049 kb on - strand, within Pf6N2E2_2378 at 2817.049 kb on - strand, within Pf6N2E2_2378 at 2817.049 kb on - strand, within Pf6N2E2_2378 at 2817.049 kb on - strand, within Pf6N2E2_2378 at 2817.049 kb on - strand, within Pf6N2E2_2378 at 2817.049 kb on - strand, within Pf6N2E2_2378 at 2817.130 kb on - strand, within Pf6N2E2_2378 at 2817.171 kb on + strand at 2817.171 kb on + strand at 2817.172 kb on - strand at 2817.172 kb on - strand at 2817.192 kb on + strand at 2817.192 kb on + strand at 2817.192 kb on + strand at 2817.192 kb on + strand at 2817.192 kb on + strand at 2817.192 kb on + strand at 2817.192 kb on + strand at 2817.193 kb on - strand at 2817.193 kb on - strand at 2817.193 kb on - strand at 2817.193 kb on - strand at 2817.398 kb on - strand at 2817.672 kb on + strand at 2818.012 kb on + strand, within Pf6N2E2_2380 at 2818.012 kb on + strand, within Pf6N2E2_2380 at 2818.012 kb on + strand, within Pf6N2E2_2380 at 2818.013 kb on - strand, within Pf6N2E2_2380 at 2818.013 kb on - strand, within Pf6N2E2_2380 at 2818.013 kb on - strand, within Pf6N2E2_2380 at 2818.042 kb on + strand, within Pf6N2E2_2380 at 2818.042 kb on + strand, within Pf6N2E2_2380 at 2818.042 kb on + strand, within Pf6N2E2_2380 at 2818.043 kb on - strand, within Pf6N2E2_2380 at 2818.043 kb on - strand, within Pf6N2E2_2380 at 2818.043 kb on - strand, within Pf6N2E2_2380 at 2818.043 kb on - strand, within Pf6N2E2_2380 at 2818.043 kb on - strand, within Pf6N2E2_2380 at 2818.043 kb on - strand, within Pf6N2E2_2380 at 2818.043 kb on - strand, within Pf6N2E2_2380 at 2818.043 kb on - strand, within Pf6N2E2_2380 at 2818.043 kb on - strand, within Pf6N2E2_2380 at 2818.043 kb on - strand, within Pf6N2E2_2380 at 2818.043 kb on - strand, within Pf6N2E2_2380 at 2818.045 kb on + strand, within Pf6N2E2_2380 at 2818.045 kb on + strand, within Pf6N2E2_2380 at 2818.045 kb on + strand, within Pf6N2E2_2380 at 2818.046 kb on - strand, within Pf6N2E2_2380 at 2818.046 kb on - strand, within Pf6N2E2_2380 at 2818.046 kb on - strand, within Pf6N2E2_2380 at 2818.046 kb on - strand, within Pf6N2E2_2380 at 2818.046 kb on - strand, within Pf6N2E2_2380 at 2818.046 kb on - strand, within Pf6N2E2_2380 at 2818.046 kb on - strand, within Pf6N2E2_2380 at 2818.086 kb on + strand, within Pf6N2E2_2380 at 2818.136 kb on - strand, within Pf6N2E2_2380 at 2818.136 kb on - strand, within Pf6N2E2_2380
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 2,813,938 + Pf6N2E2_2377 0.68 +0.7 2,813,938 + Pf6N2E2_2377 0.68 +1.5 2,813,939 - Pf6N2E2_2377 0.68 +0.5 2,814,016 + Pf6N2E2_2377 0.72 -0.2 2,814,070 + Pf6N2E2_2377 0.76 +0.2 2,814,070 + Pf6N2E2_2377 0.76 +0.3 2,814,070 + Pf6N2E2_2377 0.76 -0.0 2,814,070 + Pf6N2E2_2377 0.76 +0.3 2,814,071 - Pf6N2E2_2377 0.76 +0.4 2,814,071 - Pf6N2E2_2377 0.76 +1.5 2,814,071 - Pf6N2E2_2377 0.76 +0.4 2,814,071 - Pf6N2E2_2377 0.76 -0.6 2,814,071 - Pf6N2E2_2377 0.76 -0.8 2,814,071 - Pf6N2E2_2377 0.76 -1.0 2,814,071 - Pf6N2E2_2377 0.76 +1.1 2,814,071 - Pf6N2E2_2377 0.76 -2.2 2,814,124 + Pf6N2E2_2377 0.79 +2.5 2,814,125 - Pf6N2E2_2377 0.79 +0.6 2,814,145 + Pf6N2E2_2377 0.80 -0.4 2,814,145 + Pf6N2E2_2377 0.80 +0.1 2,814,145 + Pf6N2E2_2377 0.80 -0.5 2,814,146 - Pf6N2E2_2377 0.80 -0.5 2,814,146 - Pf6N2E2_2377 0.80 -0.4 2,814,146 - Pf6N2E2_2377 0.80 +0.5 2,814,365 + +0.3 2,814,365 + -0.6 2,814,480 - -0.1 2,814,497 + -0.6 2,814,620 + +0.1 2,814,668 + +0.3 2,814,669 - +0.1 2,814,669 - -0.1 2,814,762 + -1.8 2,814,762 + +0.5 2,814,763 - -1.7 2,814,763 - -0.3 2,814,763 - +1.5 2,814,763 - +1.3 2,814,766 + +0.6 2,814,766 + -0.3 2,814,766 + +0.1 2,814,766 + -0.5 2,814,766 + -0.3 2,814,767 - +0.0 2,814,767 - +0.4 2,814,767 - -0.4 2,814,767 - -0.1 2,814,767 - -0.2 2,814,767 - -2.9 2,815,020 + -0.6 2,815,021 - -0.8 2,815,059 + +0.0 2,815,059 + -1.3 2,815,059 + +1.1 2,815,060 - +0.7 2,815,060 - -0.9 2,815,060 - +1.2 2,815,137 + -0.0 2,815,137 + -0.5 2,815,236 + Pf6N2E2_2378 0.12 -1.6 2,815,236 + Pf6N2E2_2378 0.12 +0.2 2,815,741 - Pf6N2E2_2378 0.33 +1.5 2,815,741 - Pf6N2E2_2378 0.33 -2.1 2,815,755 + Pf6N2E2_2378 0.33 -0.1 2,815,755 + Pf6N2E2_2378 0.33 -0.1 2,815,755 + Pf6N2E2_2378 0.33 +1.5 2,815,755 + Pf6N2E2_2378 0.33 -0.1 2,815,756 - Pf6N2E2_2378 0.34 +0.7 2,815,756 - Pf6N2E2_2378 0.34 -0.9 2,815,869 + Pf6N2E2_2378 0.38 +1.5 2,815,869 + Pf6N2E2_2378 0.38 +1.1 2,815,869 + Pf6N2E2_2378 0.38 -1.3 2,815,870 - Pf6N2E2_2378 0.38 +1.2 2,815,870 - Pf6N2E2_2378 0.38 +0.7 2,815,870 - Pf6N2E2_2378 0.38 +1.5 2,815,870 - Pf6N2E2_2378 0.38 +1.2 2,815,992 + Pf6N2E2_2378 0.43 +1.2 2,816,067 + Pf6N2E2_2378 0.46 +0.5 2,816,067 + Pf6N2E2_2378 0.46 -0.5 2,816,067 + Pf6N2E2_2378 0.46 -1.1 2,816,329 + Pf6N2E2_2378 0.57 +0.1 2,816,330 - Pf6N2E2_2378 0.57 -0.0 2,816,481 + Pf6N2E2_2378 0.63 +1.5 2,816,535 + Pf6N2E2_2378 0.65 -0.1 2,816,535 + Pf6N2E2_2378 0.65 +0.5 2,816,535 + Pf6N2E2_2378 0.65 -0.6 2,816,536 - Pf6N2E2_2378 0.65 +0.3 2,816,536 - Pf6N2E2_2378 0.65 +1.0 2,816,536 - Pf6N2E2_2378 0.65 +0.0 2,816,536 - Pf6N2E2_2378 0.65 +0.5 2,816,536 - Pf6N2E2_2378 0.65 -0.5 2,816,538 + Pf6N2E2_2378 0.65 +0.8 2,816,539 - Pf6N2E2_2378 0.65 -0.2 2,816,539 - Pf6N2E2_2378 0.65 +0.1 2,816,635 - Pf6N2E2_2378 0.69 -0.2 2,816,713 - Pf6N2E2_2378 0.73 +0.9 2,816,713 - Pf6N2E2_2378 0.73 -0.1 2,816,713 - Pf6N2E2_2378 0.73 +0.5 2,816,713 - Pf6N2E2_2378 0.73 -0.2 2,816,719 + Pf6N2E2_2378 0.73 +0.9 2,816,719 + Pf6N2E2_2378 0.73 -0.3 2,816,720 - Pf6N2E2_2378 0.73 -0.4 2,817,048 + Pf6N2E2_2378 0.86 -0.2 2,817,048 + Pf6N2E2_2378 0.86 -0.5 2,817,048 + Pf6N2E2_2378 0.86 -0.9 2,817,048 + Pf6N2E2_2378 0.86 +0.1 2,817,048 + Pf6N2E2_2378 0.86 -0.7 2,817,048 + Pf6N2E2_2378 0.86 -0.7 2,817,048 + Pf6N2E2_2378 0.86 +0.4 2,817,048 + Pf6N2E2_2378 0.86 -0.5 2,817,049 - Pf6N2E2_2378 0.86 -0.4 2,817,049 - Pf6N2E2_2378 0.86 -0.7 2,817,049 - Pf6N2E2_2378 0.86 -0.1 2,817,049 - Pf6N2E2_2378 0.86 +0.1 2,817,049 - Pf6N2E2_2378 0.86 -0.0 2,817,049 - Pf6N2E2_2378 0.86 +0.9 2,817,049 - Pf6N2E2_2378 0.86 -0.1 2,817,130 - Pf6N2E2_2378 0.90 +0.3 2,817,171 + -1.1 2,817,171 + -0.4 2,817,172 - +2.1 2,817,172 - -0.1 2,817,192 + -0.5 2,817,192 + +0.5 2,817,192 + +1.5 2,817,192 + +0.4 2,817,192 + +0.1 2,817,192 + +0.8 2,817,192 + +0.3 2,817,193 - -0.4 2,817,193 - -1.1 2,817,193 - -0.3 2,817,193 - -0.1 2,817,398 - +0.1 2,817,672 + +0.2 2,818,012 + Pf6N2E2_2380 0.11 -0.1 2,818,012 + Pf6N2E2_2380 0.11 +0.4 2,818,012 + Pf6N2E2_2380 0.11 +1.3 2,818,013 - Pf6N2E2_2380 0.12 +0.5 2,818,013 - Pf6N2E2_2380 0.12 -0.4 2,818,013 - Pf6N2E2_2380 0.12 -0.5 2,818,042 + Pf6N2E2_2380 0.13 -0.1 2,818,042 + Pf6N2E2_2380 0.13 +0.7 2,818,042 + Pf6N2E2_2380 0.13 -0.4 2,818,043 - Pf6N2E2_2380 0.13 -0.2 2,818,043 - Pf6N2E2_2380 0.13 -0.7 2,818,043 - Pf6N2E2_2380 0.13 +0.5 2,818,043 - Pf6N2E2_2380 0.13 -1.3 2,818,043 - Pf6N2E2_2380 0.13 -1.4 2,818,043 - Pf6N2E2_2380 0.13 +0.6 2,818,043 - Pf6N2E2_2380 0.13 -2.0 2,818,043 - Pf6N2E2_2380 0.13 +0.8 2,818,043 - Pf6N2E2_2380 0.13 +0.2 2,818,043 - Pf6N2E2_2380 0.13 -1.1 2,818,043 - Pf6N2E2_2380 0.13 -0.1 2,818,045 + Pf6N2E2_2380 0.13 -0.7 2,818,045 + Pf6N2E2_2380 0.13 +0.2 2,818,045 + Pf6N2E2_2380 0.13 +0.6 2,818,046 - Pf6N2E2_2380 0.13 -1.8 2,818,046 - Pf6N2E2_2380 0.13 +0.1 2,818,046 - Pf6N2E2_2380 0.13 +0.5 2,818,046 - Pf6N2E2_2380 0.13 -1.1 2,818,046 - Pf6N2E2_2380 0.13 +1.5 2,818,046 - Pf6N2E2_2380 0.13 -0.2 2,818,046 - Pf6N2E2_2380 0.13 -0.9 2,818,086 + Pf6N2E2_2380 0.14 +0.9 2,818,136 - Pf6N2E2_2380 0.16 +1.5 2,818,136 - Pf6N2E2_2380 0.16 +0.2
Or see this region's nucleotide sequence