Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2374 and Pf6N2E2_2375 are separated by 14 nucleotides Pf6N2E2_2375 and Pf6N2E2_2376 are separated by 370 nucleotides Pf6N2E2_2376 and Pf6N2E2_2377 are separated by 9 nucleotides
Pf6N2E2_2374 - Nitrate ABC transporter, nitrate-binding protein, at 2,809,411 to 2,810,622
_2374
Pf6N2E2_2375 - Response regulator NasT, at 2,810,637 to 2,811,212
_2375
Pf6N2E2_2376 - nitrate transporter (from data) , at 2,811,583 to 2,812,794
_2376
Pf6N2E2_2377 - Serine/threonine protein kinase (EC 2.7.11.1), at 2,812,804 to 2,814,477
_2377
Position (kb)
2811
2812
2813 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2810.646 kb on - strand at 2811.120 kb on + strand, within Pf6N2E2_2375 at 2811.120 kb on + strand, within Pf6N2E2_2375 at 2811.120 kb on + strand, within Pf6N2E2_2375 at 2811.121 kb on - strand, within Pf6N2E2_2375 at 2811.241 kb on + strand at 2811.352 kb on - strand at 2811.352 kb on - strand at 2811.388 kb on + strand at 2811.404 kb on + strand at 2811.567 kb on - strand at 2811.640 kb on + strand at 2811.640 kb on + strand at 2811.640 kb on + strand at 2811.640 kb on + strand at 2811.641 kb on - strand at 2811.886 kb on + strand, within Pf6N2E2_2376 at 2811.886 kb on + strand, within Pf6N2E2_2376 at 2811.886 kb on + strand, within Pf6N2E2_2376 at 2812.187 kb on - strand, within Pf6N2E2_2376 at 2812.234 kb on + strand, within Pf6N2E2_2376 at 2812.300 kb on + strand, within Pf6N2E2_2376 at 2812.328 kb on - strand, within Pf6N2E2_2376 at 2812.330 kb on + strand, within Pf6N2E2_2376 at 2812.330 kb on + strand, within Pf6N2E2_2376 at 2812.330 kb on + strand, within Pf6N2E2_2376 at 2812.330 kb on + strand, within Pf6N2E2_2376 at 2812.331 kb on - strand, within Pf6N2E2_2376 at 2812.331 kb on - strand, within Pf6N2E2_2376 at 2812.331 kb on - strand, within Pf6N2E2_2376 at 2812.331 kb on - strand, within Pf6N2E2_2376 at 2812.331 kb on - strand, within Pf6N2E2_2376 at 2812.331 kb on - strand, within Pf6N2E2_2376 at 2812.331 kb on - strand, within Pf6N2E2_2376 at 2812.331 kb on - strand, within Pf6N2E2_2376 at 2812.426 kb on + strand, within Pf6N2E2_2376 at 2812.426 kb on + strand, within Pf6N2E2_2376 at 2812.426 kb on + strand, within Pf6N2E2_2376 at 2812.426 kb on + strand, within Pf6N2E2_2376 at 2812.426 kb on + strand, within Pf6N2E2_2376 at 2812.426 kb on + strand, within Pf6N2E2_2376 at 2812.426 kb on + strand, within Pf6N2E2_2376 at 2812.426 kb on + strand, within Pf6N2E2_2376 at 2812.426 kb on + strand, within Pf6N2E2_2376 at 2812.427 kb on - strand, within Pf6N2E2_2376 at 2812.427 kb on - strand, within Pf6N2E2_2376 at 2812.427 kb on - strand, within Pf6N2E2_2376 at 2812.427 kb on - strand, within Pf6N2E2_2376 at 2812.427 kb on - strand, within Pf6N2E2_2376 at 2812.427 kb on - strand, within Pf6N2E2_2376 at 2812.427 kb on - strand, within Pf6N2E2_2376 at 2812.427 kb on - strand, within Pf6N2E2_2376 at 2812.481 kb on - strand, within Pf6N2E2_2376 at 2812.790 kb on + strand at 2812.792 kb on + strand at 2812.792 kb on + strand at 2812.793 kb on - strand at 2812.793 kb on - strand at 2812.793 kb on - strand at 2812.793 kb on - strand at 2812.793 kb on - strand at 2812.793 kb on - strand at 2812.793 kb on - strand at 2812.796 kb on + strand at 2812.796 kb on + strand at 2812.796 kb on + strand at 2812.916 kb on - strand at 2813.002 kb on + strand, within Pf6N2E2_2377 at 2813.002 kb on + strand, within Pf6N2E2_2377 at 2813.002 kb on + strand, within Pf6N2E2_2377 at 2813.003 kb on - strand, within Pf6N2E2_2377 at 2813.003 kb on - strand, within Pf6N2E2_2377 at 2813.006 kb on - strand, within Pf6N2E2_2377 at 2813.006 kb on - strand, within Pf6N2E2_2377 at 2813.331 kb on - strand, within Pf6N2E2_2377 at 2813.331 kb on - strand, within Pf6N2E2_2377 at 2813.331 kb on - strand, within Pf6N2E2_2377 at 2813.663 kb on + strand, within Pf6N2E2_2377
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 2,810,646 - +0.1 2,811,120 + Pf6N2E2_2375 0.84 -0.3 2,811,120 + Pf6N2E2_2375 0.84 -0.6 2,811,120 + Pf6N2E2_2375 0.84 -2.5 2,811,121 - Pf6N2E2_2375 0.84 +1.0 2,811,241 + -0.5 2,811,352 - +1.5 2,811,352 - +0.0 2,811,388 + -0.4 2,811,404 + +0.2 2,811,567 - +0.1 2,811,640 + -0.8 2,811,640 + -0.2 2,811,640 + +0.1 2,811,640 + -0.2 2,811,641 - +0.7 2,811,886 + Pf6N2E2_2376 0.25 -1.1 2,811,886 + Pf6N2E2_2376 0.25 -0.4 2,811,886 + Pf6N2E2_2376 0.25 +1.5 2,812,187 - Pf6N2E2_2376 0.50 -1.5 2,812,234 + Pf6N2E2_2376 0.54 -0.0 2,812,300 + Pf6N2E2_2376 0.59 -3.3 2,812,328 - Pf6N2E2_2376 0.61 -0.3 2,812,330 + Pf6N2E2_2376 0.62 +0.5 2,812,330 + Pf6N2E2_2376 0.62 +0.1 2,812,330 + Pf6N2E2_2376 0.62 +1.3 2,812,330 + Pf6N2E2_2376 0.62 -1.1 2,812,331 - Pf6N2E2_2376 0.62 +3.5 2,812,331 - Pf6N2E2_2376 0.62 +1.5 2,812,331 - Pf6N2E2_2376 0.62 +0.3 2,812,331 - Pf6N2E2_2376 0.62 -0.8 2,812,331 - Pf6N2E2_2376 0.62 -0.1 2,812,331 - Pf6N2E2_2376 0.62 +0.5 2,812,331 - Pf6N2E2_2376 0.62 +0.1 2,812,331 - Pf6N2E2_2376 0.62 +1.5 2,812,426 + Pf6N2E2_2376 0.70 -0.3 2,812,426 + Pf6N2E2_2376 0.70 -1.5 2,812,426 + Pf6N2E2_2376 0.70 +0.5 2,812,426 + Pf6N2E2_2376 0.70 -0.8 2,812,426 + Pf6N2E2_2376 0.70 +1.8 2,812,426 + Pf6N2E2_2376 0.70 -1.7 2,812,426 + Pf6N2E2_2376 0.70 -0.3 2,812,426 + Pf6N2E2_2376 0.70 +0.4 2,812,426 + Pf6N2E2_2376 0.70 +0.5 2,812,427 - Pf6N2E2_2376 0.70 -0.5 2,812,427 - Pf6N2E2_2376 0.70 -1.1 2,812,427 - Pf6N2E2_2376 0.70 +0.3 2,812,427 - Pf6N2E2_2376 0.70 -0.2 2,812,427 - Pf6N2E2_2376 0.70 -0.5 2,812,427 - Pf6N2E2_2376 0.70 +0.0 2,812,427 - Pf6N2E2_2376 0.70 -1.0 2,812,427 - Pf6N2E2_2376 0.70 -0.9 2,812,481 - Pf6N2E2_2376 0.74 +0.3 2,812,790 + -0.1 2,812,792 + -0.1 2,812,792 + -0.6 2,812,793 - +0.2 2,812,793 - +0.1 2,812,793 - -0.2 2,812,793 - -0.1 2,812,793 - +0.3 2,812,793 - +0.3 2,812,793 - +0.1 2,812,796 + -0.3 2,812,796 + -0.2 2,812,796 + +0.1 2,812,916 - -2.4 2,813,002 + Pf6N2E2_2377 0.12 -0.1 2,813,002 + Pf6N2E2_2377 0.12 -0.2 2,813,002 + Pf6N2E2_2377 0.12 +1.5 2,813,003 - Pf6N2E2_2377 0.12 -1.1 2,813,003 - Pf6N2E2_2377 0.12 -0.2 2,813,006 - Pf6N2E2_2377 0.12 -1.9 2,813,006 - Pf6N2E2_2377 0.12 -0.2 2,813,331 - Pf6N2E2_2377 0.31 -0.4 2,813,331 - Pf6N2E2_2377 0.31 +0.9 2,813,331 - Pf6N2E2_2377 0.31 -0.7 2,813,663 + Pf6N2E2_2377 0.51 -0.1
Or see this region's nucleotide sequence