Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2374

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
2,808,447	-				+0.3
2,808,447	-				-0.8
2,808,447	-				+1.5
2,808,447	-				-1.4
2,808,727	+		Pf6N2E2_2373	0.40	-0.1
2,808,736	+		Pf6N2E2_2373	0.41	-0.1
2,808,786	-		Pf6N2E2_2373	0.48	-0.6
2,809,183	+				-1.4
2,809,183	+				-0.9
2,809,184	-				-2.2
2,809,184	-				-0.7
2,809,187	+				+1.3
2,809,187	+				-0.4
2,809,187	+				-0.4
2,809,187	+				-0.8
2,809,187	+				-1.6
2,809,188	-				-0.3
2,809,265	+				+0.3
2,809,265	+				+1.5
2,809,265	+				-0.8
2,809,266	-				-0.8
2,809,266	-				-0.7
2,809,266	-				+0.1
2,809,331	-				+0.6
2,809,657	+		Pf6N2E2_2374	0.20	+0.4
2,809,657	+		Pf6N2E2_2374	0.20	+0.0
2,809,658	-		Pf6N2E2_2374	0.20	-0.3
2,809,658	-		Pf6N2E2_2374	0.20	+0.2
2,809,870	+		Pf6N2E2_2374	0.38	+0.8
2,809,870	+		Pf6N2E2_2374	0.38	-0.1
2,809,870	+		Pf6N2E2_2374	0.38	-0.6
2,809,870	+		Pf6N2E2_2374	0.38	-0.7
2,809,870	+		Pf6N2E2_2374	0.38	-0.3
2,809,870	+		Pf6N2E2_2374	0.38	-0.3
2,809,871	-		Pf6N2E2_2374	0.38	+0.4
2,809,871	-		Pf6N2E2_2374	0.38	-0.1
2,809,871	-		Pf6N2E2_2374	0.38	-0.1
2,809,871	-		Pf6N2E2_2374	0.38	+0.2
2,809,873	+		Pf6N2E2_2374	0.38	+0.3
2,809,874	-		Pf6N2E2_2374	0.38	+0.4
2,809,969	-		Pf6N2E2_2374	0.46	-0.3
2,810,347	+		Pf6N2E2_2374	0.77	-0.1
2,810,348	-		Pf6N2E2_2374	0.77	-0.4
2,810,416	+		Pf6N2E2_2374	0.83	-0.1
2,810,560	+				+1.5
2,810,646	-				+0.1
2,811,120	+		Pf6N2E2_2375	0.84	-0.3
2,811,120	+		Pf6N2E2_2375	0.84	-0.6
2,811,120	+		Pf6N2E2_2375	0.84	-2.5
2,811,121	-		Pf6N2E2_2375	0.84	+1.0
2,811,241	+				-0.5
2,811,352	-				+1.5
2,811,352	-				+0.0
2,811,388	+				-0.4
2,811,404	+				+0.2
2,811,567	-				+0.1