Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_235 and Pf6N2E2_236 are separated by 177 nucleotides Pf6N2E2_236 and Pf6N2E2_237 are separated by 291 nucleotides Pf6N2E2_237 and Pf6N2E2_238 are separated by 219 nucleotides Pf6N2E2_238 and Pf6N2E2_239 are separated by 33 nucleotides
Pf6N2E2_235 - L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1), at 297,682 to 298,422
_235
Pf6N2E2_236 - putative transcriptional regulator protein, at 298,600 to 299,253
_236
Pf6N2E2_237 - hypothetical protein, at 299,545 to 299,757
_237
Pf6N2E2_238 - hypothetical protein, at 299,977 to 300,096
_238
Pf6N2E2_239 - Fatty acid desaturase, at 300,130 to 300,831
_239
Position (kb) 298
299
300 Strain fitness (log2 ratio) -2
-1
0
1
2 at 297.663 kb on - strand at 297.812 kb on - strand, within Pf6N2E2_235 at 297.820 kb on + strand, within Pf6N2E2_235 at 297.820 kb on + strand, within Pf6N2E2_235 at 297.820 kb on + strand, within Pf6N2E2_235 at 297.821 kb on - strand, within Pf6N2E2_235 at 297.821 kb on - strand, within Pf6N2E2_235 at 297.821 kb on - strand, within Pf6N2E2_235 at 297.821 kb on - strand, within Pf6N2E2_235 at 297.821 kb on - strand, within Pf6N2E2_235 at 297.821 kb on - strand, within Pf6N2E2_235 at 297.853 kb on + strand, within Pf6N2E2_235 at 297.854 kb on - strand, within Pf6N2E2_235 at 297.991 kb on + strand, within Pf6N2E2_235 at 297.991 kb on + strand, within Pf6N2E2_235 at 297.991 kb on + strand, within Pf6N2E2_235 at 297.991 kb on + strand, within Pf6N2E2_235 at 298.023 kb on + strand, within Pf6N2E2_235 at 298.024 kb on - strand, within Pf6N2E2_235 at 298.024 kb on - strand, within Pf6N2E2_235 at 298.348 kb on + strand, within Pf6N2E2_235 at 298.351 kb on + strand at 298.351 kb on + strand at 298.351 kb on + strand at 298.351 kb on + strand at 298.352 kb on - strand at 298.440 kb on + strand at 298.441 kb on - strand at 298.553 kb on - strand at 298.716 kb on + strand, within Pf6N2E2_236 at 298.822 kb on + strand, within Pf6N2E2_236 at 299.089 kb on + strand, within Pf6N2E2_236 at 299.090 kb on - strand, within Pf6N2E2_236 at 299.116 kb on + strand, within Pf6N2E2_236 at 299.129 kb on + strand, within Pf6N2E2_236 at 299.129 kb on + strand, within Pf6N2E2_236 at 299.129 kb on + strand, within Pf6N2E2_236 at 299.130 kb on - strand, within Pf6N2E2_236 at 299.130 kb on - strand, within Pf6N2E2_236 at 299.165 kb on + strand, within Pf6N2E2_236 at 299.272 kb on + strand at 299.272 kb on + strand at 299.301 kb on - strand at 299.307 kb on + strand at 299.392 kb on + strand at 299.393 kb on - strand at 299.413 kb on + strand at 299.414 kb on - strand at 299.491 kb on + strand at 299.491 kb on + strand at 299.491 kb on + strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.538 kb on + strand at 299.539 kb on - strand at 299.539 kb on - strand at 299.539 kb on - strand at 299.544 kb on + strand at 299.544 kb on + strand at 299.544 kb on + strand at 299.544 kb on + strand at 299.545 kb on - strand at 299.545 kb on - strand at 299.591 kb on - strand, within Pf6N2E2_237 at 299.591 kb on - strand, within Pf6N2E2_237 at 299.591 kb on - strand, within Pf6N2E2_237 at 299.591 kb on - strand, within Pf6N2E2_237 at 299.747 kb on - strand at 299.786 kb on - strand at 299.810 kb on - strand at 299.810 kb on - strand at 299.810 kb on - strand at 299.810 kb on - strand at 299.810 kb on - strand at 299.813 kb on - strand at 299.813 kb on - strand at 299.871 kb on - strand at 299.880 kb on + strand at 299.880 kb on + strand at 299.880 kb on + strand at 299.881 kb on - strand at 299.881 kb on - strand at 299.881 kb on - strand at 299.896 kb on + strand at 299.978 kb on + strand at 299.979 kb on - strand at 299.992 kb on + strand, within Pf6N2E2_238 at 299.992 kb on + strand, within Pf6N2E2_238 at 300.010 kb on - strand, within Pf6N2E2_238 at 300.172 kb on + strand at 300.183 kb on + strand at 300.184 kb on - strand at 300.184 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 297,663 - -1.7 297,812 - Pf6N2E2_235 0.18 +0.3 297,820 + Pf6N2E2_235 0.19 +0.7 297,820 + Pf6N2E2_235 0.19 -0.1 297,820 + Pf6N2E2_235 0.19 -0.8 297,821 - Pf6N2E2_235 0.19 -0.8 297,821 - Pf6N2E2_235 0.19 +0.2 297,821 - Pf6N2E2_235 0.19 -0.6 297,821 - Pf6N2E2_235 0.19 +0.8 297,821 - Pf6N2E2_235 0.19 -0.4 297,821 - Pf6N2E2_235 0.19 -2.3 297,853 + Pf6N2E2_235 0.23 +0.0 297,854 - Pf6N2E2_235 0.23 +0.0 297,991 + Pf6N2E2_235 0.42 -0.5 297,991 + Pf6N2E2_235 0.42 +1.6 297,991 + Pf6N2E2_235 0.42 -0.2 297,991 + Pf6N2E2_235 0.42 -0.8 298,023 + Pf6N2E2_235 0.46 +1.6 298,024 - Pf6N2E2_235 0.46 -0.0 298,024 - Pf6N2E2_235 0.46 -0.6 298,348 + Pf6N2E2_235 0.90 +0.3 298,351 + +0.3 298,351 + -0.7 298,351 + +0.6 298,351 + -0.0 298,352 - -0.4 298,440 + -0.5 298,441 - -0.6 298,553 - +1.4 298,716 + Pf6N2E2_236 0.18 -0.0 298,822 + Pf6N2E2_236 0.34 +0.5 299,089 + Pf6N2E2_236 0.75 -0.8 299,090 - Pf6N2E2_236 0.75 -1.3 299,116 + Pf6N2E2_236 0.79 -1.7 299,129 + Pf6N2E2_236 0.81 -0.8 299,129 + Pf6N2E2_236 0.81 -0.9 299,129 + Pf6N2E2_236 0.81 -0.0 299,130 - Pf6N2E2_236 0.81 -0.0 299,130 - Pf6N2E2_236 0.81 -0.1 299,165 + Pf6N2E2_236 0.86 -2.0 299,272 + -1.0 299,272 + -0.0 299,301 - -0.1 299,307 + +0.1 299,392 + -1.1 299,393 - -0.8 299,413 + -0.6 299,414 - -0.6 299,491 + -2.7 299,491 + -0.7 299,491 + +0.6 299,492 - +1.6 299,492 - -0.9 299,492 - -0.4 299,492 - +0.0 299,492 - -0.7 299,492 - +0.6 299,492 - -0.6 299,492 - +0.1 299,492 - -0.7 299,538 + +0.6 299,539 - +1.6 299,539 - +0.3 299,539 - +1.6 299,544 + -0.7 299,544 + -0.2 299,544 + +0.4 299,544 + +1.6 299,545 - +0.6 299,545 - +1.6 299,591 - Pf6N2E2_237 0.22 -2.3 299,591 - Pf6N2E2_237 0.22 -0.8 299,591 - Pf6N2E2_237 0.22 +0.7 299,591 - Pf6N2E2_237 0.22 -0.6 299,747 - -0.3 299,786 - +1.1 299,810 - +0.3 299,810 - +1.1 299,810 - -1.0 299,810 - +1.0 299,810 - -0.8 299,813 - +0.2 299,813 - +0.6 299,871 - +0.6 299,880 + +1.6 299,880 + -1.7 299,880 + +0.5 299,881 - +0.1 299,881 - -1.9 299,881 - -0.2 299,896 + -0.4 299,978 + -0.6 299,979 - -0.4 299,992 + Pf6N2E2_238 0.12 +0.6 299,992 + Pf6N2E2_238 0.12 -1.0 300,010 - Pf6N2E2_238 0.28 +1.6 300,172 + +2.6 300,183 + +1.6 300,184 - +0.6 300,184 - -0.7
Or see this region's nucleotide sequence