Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_233

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_232 and Pf6N2E2_233 are separated by 144 nucleotidesPf6N2E2_233 and Pf6N2E2_234 are separated by 229 nucleotidesPf6N2E2_234 and Pf6N2E2_235 are separated by 181 nucleotides Pf6N2E2_232 - Protein involved in meta-pathway of phenol degradation, at 295,139 to 296,131 _232 Pf6N2E2_233 - Transcriptional regulator, TetR family, at 296,276 to 296,896 _233 Pf6N2E2_234 - FIG00454312: hypothetical protein, at 297,126 to 297,500 _234 Pf6N2E2_235 - L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1), at 297,682 to 298,422 _235 Position (kb) 296 297Strain fitness (log2 ratio) -2 -1 0 1at 295.341 kb on + strand, within Pf6N2E2_232at 295.341 kb on + strand, within Pf6N2E2_232at 295.342 kb on - strand, within Pf6N2E2_232at 295.344 kb on + strand, within Pf6N2E2_232at 295.345 kb on - strand, within Pf6N2E2_232at 295.384 kb on - strand, within Pf6N2E2_232at 295.384 kb on - strand, within Pf6N2E2_232at 295.494 kb on + strand, within Pf6N2E2_232at 295.494 kb on + strand, within Pf6N2E2_232at 295.495 kb on - strand, within Pf6N2E2_232at 295.495 kb on - strand, within Pf6N2E2_232at 295.495 kb on - strand, within Pf6N2E2_232at 295.495 kb on - strand, within Pf6N2E2_232at 295.500 kb on + strand, within Pf6N2E2_232at 295.500 kb on + strand, within Pf6N2E2_232at 295.500 kb on + strand, within Pf6N2E2_232at 295.612 kb on - strand, within Pf6N2E2_232at 295.612 kb on - strand, within Pf6N2E2_232at 295.845 kb on + strand, within Pf6N2E2_232at 295.846 kb on - strand, within Pf6N2E2_232at 295.846 kb on - strand, within Pf6N2E2_232at 295.848 kb on + strand, within Pf6N2E2_232at 295.848 kb on + strand, within Pf6N2E2_232at 295.849 kb on - strand, within Pf6N2E2_232at 295.849 kb on - strand, within Pf6N2E2_232at 295.849 kb on - strand, within Pf6N2E2_232at 295.956 kb on + strand, within Pf6N2E2_232at 295.956 kb on + strand, within Pf6N2E2_232at 295.957 kb on - strand, within Pf6N2E2_232at 295.957 kb on - strand, within Pf6N2E2_232at 295.957 kb on - strand, within Pf6N2E2_232at 296.190 kb on + strandat 296.190 kb on + strandat 296.191 kb on - strandat 296.376 kb on + strand, within Pf6N2E2_233at 296.376 kb on + strand, within Pf6N2E2_233at 296.377 kb on - strand, within Pf6N2E2_233at 296.377 kb on - strand, within Pf6N2E2_233at 296.649 kb on + strand, within Pf6N2E2_233at 296.649 kb on + strand, within Pf6N2E2_233at 296.650 kb on - strand, within Pf6N2E2_233at 296.650 kb on - strand, within Pf6N2E2_233at 296.691 kb on + strand, within Pf6N2E2_233at 296.691 kb on + strand, within Pf6N2E2_233at 296.691 kb on + strand, within Pf6N2E2_233at 296.691 kb on + strand, within Pf6N2E2_233at 296.691 kb on + strand, within Pf6N2E2_233at 296.692 kb on - strand, within Pf6N2E2_233at 296.692 kb on - strand, within Pf6N2E2_233at 296.692 kb on - strand, within Pf6N2E2_233at 296.733 kb on + strand, within Pf6N2E2_233at 296.733 kb on + strand, within Pf6N2E2_233at 296.733 kb on + strand, within Pf6N2E2_233at 296.733 kb on + strand, within Pf6N2E2_233at 296.733 kb on + strand, within Pf6N2E2_233at 296.733 kb on + strand, within Pf6N2E2_233at 296.734 kb on - strand, within Pf6N2E2_233at 296.734 kb on - strand, within Pf6N2E2_233at 296.734 kb on - strand, within Pf6N2E2_233at 296.734 kb on - strand, within Pf6N2E2_233at 296.738 kb on - strand, within Pf6N2E2_233at 296.792 kb on + strand, within Pf6N2E2_233at 296.792 kb on + strand, within Pf6N2E2_233at 296.793 kb on - strand, within Pf6N2E2_233at 296.793 kb on - strand, within Pf6N2E2_233at 296.793 kb on - strand, within Pf6N2E2_233at 296.793 kb on - strand, within Pf6N2E2_233at 296.793 kb on - strand, within Pf6N2E2_233at 296.793 kb on - strand, within Pf6N2E2_233at 296.793 kb on - strand, within Pf6N2E2_233at 296.806 kb on + strand, within Pf6N2E2_233at 296.833 kb on + strand, within Pf6N2E2_233at 296.833 kb on + strand, within Pf6N2E2_233at 296.834 kb on - strand, within Pf6N2E2_233at 296.926 kb on + strandat 296.926 kb on + strandat 296.927 kb on - strandat 296.927 kb on - strandat 297.081 kb on + strandat 297.081 kb on + strandat 297.081 kb on + strandat 297.081 kb on + strandat 297.114 kb on - strandat 297.114 kb on - strandat 297.114 kb on - strandat 297.135 kb on + strandat 297.254 kb on + strand, within Pf6N2E2_234at 297.342 kb on + strand, within Pf6N2E2_234at 297.342 kb on + strand, within Pf6N2E2_234at 297.342 kb on + strand, within Pf6N2E2_234at 297.342 kb on + strand, within Pf6N2E2_234at 297.464 kb on + strandat 297.499 kb on - strandat 297.663 kb on - strandat 297.812 kb on - strand, within Pf6N2E2_235at 297.820 kb on + strand, within Pf6N2E2_235at 297.820 kb on + strand, within Pf6N2E2_235at 297.820 kb on + strand, within Pf6N2E2_235at 297.821 kb on - strand, within Pf6N2E2_235at 297.821 kb on - strand, within Pf6N2E2_235at 297.821 kb on - strand, within Pf6N2E2_235at 297.821 kb on - strand, within Pf6N2E2_235at 297.821 kb on - strand, within Pf6N2E2_235at 297.821 kb on - strand, within Pf6N2E2_235at 297.853 kb on + strand, within Pf6N2E2_235at 297.854 kb on - strand, within Pf6N2E2_235

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
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295,341 + Pf6N2E2_232 0.20 +1.0
295,341 + Pf6N2E2_232 0.20 +0.7
295,342 - Pf6N2E2_232 0.20 -1.1
295,344 + Pf6N2E2_232 0.21 +1.6
295,345 - Pf6N2E2_232 0.21 -0.3
295,384 - Pf6N2E2_232 0.25 -0.1
295,384 - Pf6N2E2_232 0.25 -0.5
295,494 + Pf6N2E2_232 0.36 -0.4
295,494 + Pf6N2E2_232 0.36 +0.2
295,495 - Pf6N2E2_232 0.36 +1.6
295,495 - Pf6N2E2_232 0.36 -0.0
295,495 - Pf6N2E2_232 0.36 -2.3
295,495 - Pf6N2E2_232 0.36 +0.3
295,500 + Pf6N2E2_232 0.36 +0.2
295,500 + Pf6N2E2_232 0.36 -0.2
295,500 + Pf6N2E2_232 0.36 -0.3
295,612 - Pf6N2E2_232 0.48 -0.8
295,612 - Pf6N2E2_232 0.48 +0.7
295,845 + Pf6N2E2_232 0.71 -1.1
295,846 - Pf6N2E2_232 0.71 +0.6
295,846 - Pf6N2E2_232 0.71 -1.7
295,848 + Pf6N2E2_232 0.71 -0.3
295,848 + Pf6N2E2_232 0.71 -0.2
295,849 - Pf6N2E2_232 0.72 -0.4
295,849 - Pf6N2E2_232 0.72 -1.3
295,849 - Pf6N2E2_232 0.72 -0.4
295,956 + Pf6N2E2_232 0.82 +0.2
295,956 + Pf6N2E2_232 0.82 -0.4
295,957 - Pf6N2E2_232 0.82 +0.6
295,957 - Pf6N2E2_232 0.82 -0.8
295,957 - Pf6N2E2_232 0.82 -0.3
296,190 + +0.5
296,190 + +0.6
296,191 - +0.0
296,376 + Pf6N2E2_233 0.16 +0.6
296,376 + Pf6N2E2_233 0.16 +1.6
296,377 - Pf6N2E2_233 0.16 -0.1
296,377 - Pf6N2E2_233 0.16 +0.6
296,649 + Pf6N2E2_233 0.60 -0.7
296,649 + Pf6N2E2_233 0.60 -0.4
296,650 - Pf6N2E2_233 0.60 -1.0
296,650 - Pf6N2E2_233 0.60 -1.2
296,691 + Pf6N2E2_233 0.67 -0.0
296,691 + Pf6N2E2_233 0.67 -0.2
296,691 + Pf6N2E2_233 0.67 -0.7
296,691 + Pf6N2E2_233 0.67 -0.8
296,691 + Pf6N2E2_233 0.67 +1.6
296,692 - Pf6N2E2_233 0.67 +0.6
296,692 - Pf6N2E2_233 0.67 -0.5
296,692 - Pf6N2E2_233 0.67 -0.9
296,733 + Pf6N2E2_233 0.74 -0.8
296,733 + Pf6N2E2_233 0.74 -0.5
296,733 + Pf6N2E2_233 0.74 -0.0
296,733 + Pf6N2E2_233 0.74 -1.2
296,733 + Pf6N2E2_233 0.74 +0.9
296,733 + Pf6N2E2_233 0.74 -0.4
296,734 - Pf6N2E2_233 0.74 -0.3
296,734 - Pf6N2E2_233 0.74 -1.5
296,734 - Pf6N2E2_233 0.74 -0.9
296,734 - Pf6N2E2_233 0.74 -0.2
296,738 - Pf6N2E2_233 0.74 -0.6
296,792 + Pf6N2E2_233 0.83 +0.0
296,792 + Pf6N2E2_233 0.83 -0.7
296,793 - Pf6N2E2_233 0.83 -1.5
296,793 - Pf6N2E2_233 0.83 -0.4
296,793 - Pf6N2E2_233 0.83 -0.4
296,793 - Pf6N2E2_233 0.83 -0.3
296,793 - Pf6N2E2_233 0.83 +0.2
296,793 - Pf6N2E2_233 0.83 -0.0
296,793 - Pf6N2E2_233 0.83 -0.4
296,806 + Pf6N2E2_233 0.85 -0.8
296,833 + Pf6N2E2_233 0.90 +0.3
296,833 + Pf6N2E2_233 0.90 +0.1
296,834 - Pf6N2E2_233 0.90 -0.7
296,926 + -1.0
296,926 + -0.0
296,927 - -0.5
296,927 - -1.2
297,081 + -0.4
297,081 + +0.7
297,081 + -1.4
297,081 + +0.6
297,114 - -0.2
297,114 - -0.5
297,114 - -0.4
297,135 + -0.3
297,254 + Pf6N2E2_234 0.34 -2.5
297,342 + Pf6N2E2_234 0.58 -0.4
297,342 + Pf6N2E2_234 0.58 -0.3
297,342 + Pf6N2E2_234 0.58 -2.0
297,342 + Pf6N2E2_234 0.58 -0.0
297,464 + -0.2
297,499 - -0.8
297,663 - -1.7
297,812 - Pf6N2E2_235 0.18 +0.3
297,820 + Pf6N2E2_235 0.19 +0.7
297,820 + Pf6N2E2_235 0.19 -0.1
297,820 + Pf6N2E2_235 0.19 -0.8
297,821 - Pf6N2E2_235 0.19 -0.8
297,821 - Pf6N2E2_235 0.19 +0.2
297,821 - Pf6N2E2_235 0.19 -0.6
297,821 - Pf6N2E2_235 0.19 +0.8
297,821 - Pf6N2E2_235 0.19 -0.4
297,821 - Pf6N2E2_235 0.19 -2.3
297,853 + Pf6N2E2_235 0.23 +0.0
297,854 - Pf6N2E2_235 0.23 +0.0

Or see this region's nucleotide sequence