Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2291 and Pf6N2E2_2292 are separated by 189 nucleotides Pf6N2E2_2292 and Pf6N2E2_2293 are separated by 133 nucleotides
Pf6N2E2_2291 - FIG00953628: hypothetical protein, at 2,711,883 to 2,712,233
_2291
Pf6N2E2_2292 - Universal stress protein family COG0589, at 2,712,423 to 2,712,863
_2292
Pf6N2E2_2293 - COG0488: ATPase components of ABC transporters with duplicated ATPase domains, at 2,712,997 to 2,714,907
_2293
Position (kb)
2712
2713 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2711.429 kb on - strand at 2711.429 kb on - strand at 2711.429 kb on - strand at 2711.480 kb on - strand at 2711.527 kb on - strand at 2711.664 kb on - strand at 2711.664 kb on - strand at 2711.692 kb on + strand at 2711.692 kb on + strand at 2711.692 kb on + strand at 2711.692 kb on + strand at 2711.692 kb on + strand at 2711.692 kb on + strand at 2711.692 kb on + strand at 2711.692 kb on + strand at 2711.693 kb on - strand at 2711.693 kb on - strand at 2711.693 kb on - strand at 2711.693 kb on - strand at 2711.693 kb on - strand at 2711.693 kb on - strand at 2711.696 kb on + strand at 2711.696 kb on + strand at 2711.696 kb on + strand at 2711.696 kb on + strand at 2711.696 kb on + strand at 2711.696 kb on + strand at 2711.696 kb on + strand at 2711.697 kb on - strand at 2711.697 kb on - strand at 2711.697 kb on - strand at 2711.697 kb on - strand at 2711.697 kb on - strand at 2711.697 kb on - strand at 2711.697 kb on - strand at 2711.697 kb on - strand at 2711.702 kb on + strand at 2711.703 kb on - strand at 2711.703 kb on - strand at 2711.711 kb on + strand at 2711.711 kb on + strand at 2711.711 kb on + strand at 2711.711 kb on + strand at 2711.711 kb on + strand at 2711.716 kb on - strand at 2711.758 kb on + strand at 2711.800 kb on + strand at 2711.877 kb on + strand at 2711.877 kb on + strand at 2711.878 kb on - strand at 2711.986 kb on + strand, within Pf6N2E2_2291 at 2712.086 kb on - strand, within Pf6N2E2_2291 at 2712.279 kb on + strand at 2712.280 kb on - strand at 2712.321 kb on - strand at 2712.326 kb on + strand at 2712.388 kb on + strand at 2712.388 kb on + strand at 2712.388 kb on + strand at 2712.388 kb on + strand at 2712.388 kb on + strand at 2712.388 kb on + strand at 2712.388 kb on + strand at 2712.388 kb on + strand at 2712.388 kb on + strand at 2712.388 kb on + strand at 2712.389 kb on - strand at 2712.389 kb on - strand at 2712.389 kb on - strand at 2712.389 kb on - strand at 2712.389 kb on - strand at 2712.389 kb on - strand at 2712.389 kb on - strand at 2712.392 kb on + strand at 2712.393 kb on - strand at 2712.426 kb on + strand at 2712.426 kb on + strand at 2712.426 kb on + strand at 2712.426 kb on + strand at 2712.426 kb on + strand at 2712.427 kb on - strand at 2712.427 kb on - strand at 2712.427 kb on - strand at 2712.429 kb on + strand at 2712.429 kb on + strand at 2712.429 kb on + strand at 2712.429 kb on + strand at 2712.429 kb on + strand at 2712.430 kb on - strand at 2712.430 kb on - strand at 2712.430 kb on - strand at 2712.430 kb on - strand at 2712.430 kb on - strand at 2712.430 kb on - strand at 2712.537 kb on - strand, within Pf6N2E2_2292 at 2712.652 kb on - strand, within Pf6N2E2_2292 at 2712.652 kb on - strand, within Pf6N2E2_2292 at 2712.652 kb on - strand, within Pf6N2E2_2292 at 2712.687 kb on + strand, within Pf6N2E2_2292 at 2712.688 kb on - strand, within Pf6N2E2_2292 at 2713.100 kb on + strand at 2713.101 kb on - strand at 2713.101 kb on - strand at 2713.130 kb on - strand at 2713.199 kb on + strand, within Pf6N2E2_2293 at 2713.199 kb on + strand, within Pf6N2E2_2293 at 2713.200 kb on - strand, within Pf6N2E2_2293 at 2713.200 kb on - strand, within Pf6N2E2_2293 at 2713.200 kb on - strand, within Pf6N2E2_2293 at 2713.200 kb on - strand, within Pf6N2E2_2293 at 2713.647 kb on - strand, within Pf6N2E2_2293 at 2713.757 kb on + strand, within Pf6N2E2_2293
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 2,711,429 - +0.1 2,711,429 - +0.9 2,711,429 - +2.5 2,711,480 - -0.8 2,711,527 - -2.3 2,711,664 - -1.1 2,711,664 - +0.4 2,711,692 + -2.3 2,711,692 + -0.0 2,711,692 + +0.2 2,711,692 + +0.3 2,711,692 + -0.3 2,711,692 + -1.4 2,711,692 + +0.2 2,711,692 + -1.3 2,711,693 - -0.3 2,711,693 - -1.0 2,711,693 - -0.1 2,711,693 - -0.7 2,711,693 - -0.8 2,711,693 - +0.3 2,711,696 + -0.0 2,711,696 + -0.0 2,711,696 + +0.1 2,711,696 + +0.2 2,711,696 + +0.1 2,711,696 + -0.3 2,711,696 + -0.5 2,711,697 - -0.4 2,711,697 - -1.2 2,711,697 - -1.2 2,711,697 - +0.8 2,711,697 - -1.1 2,711,697 - +0.3 2,711,697 - -0.7 2,711,697 - -0.4 2,711,702 + +0.4 2,711,703 - -1.0 2,711,703 - +0.0 2,711,711 + -0.1 2,711,711 + -0.7 2,711,711 + -1.1 2,711,711 + -0.5 2,711,711 + -1.1 2,711,716 - +1.5 2,711,758 + -1.3 2,711,800 + +1.5 2,711,877 + +0.5 2,711,877 + -2.1 2,711,878 - +1.5 2,711,986 + Pf6N2E2_2291 0.29 +1.5 2,712,086 - Pf6N2E2_2291 0.58 +1.5 2,712,279 + +1.5 2,712,280 - +0.5 2,712,321 - +0.3 2,712,326 + +1.5 2,712,388 + +1.5 2,712,388 + +0.3 2,712,388 + -0.1 2,712,388 + -1.7 2,712,388 + -1.8 2,712,388 + -0.1 2,712,388 + -0.8 2,712,388 + +0.3 2,712,388 + -0.5 2,712,388 + -0.1 2,712,389 - -1.6 2,712,389 - +1.5 2,712,389 - +0.5 2,712,389 - -0.9 2,712,389 - -1.2 2,712,389 - -1.4 2,712,389 - -1.5 2,712,392 + -0.2 2,712,393 - +1.5 2,712,426 + +0.5 2,712,426 + -0.8 2,712,426 + +2.5 2,712,426 + -1.3 2,712,426 + +0.1 2,712,427 - -0.1 2,712,427 - -1.8 2,712,427 - +0.3 2,712,429 + -0.8 2,712,429 + -0.6 2,712,429 + -0.2 2,712,429 + -0.5 2,712,429 + -0.9 2,712,430 - -1.3 2,712,430 - -0.8 2,712,430 - +0.1 2,712,430 - -0.3 2,712,430 - +1.5 2,712,430 - -0.6 2,712,537 - Pf6N2E2_2292 0.26 -0.9 2,712,652 - Pf6N2E2_2292 0.52 +0.1 2,712,652 - Pf6N2E2_2292 0.52 -1.2 2,712,652 - Pf6N2E2_2292 0.52 +1.5 2,712,687 + Pf6N2E2_2292 0.60 -1.1 2,712,688 - Pf6N2E2_2292 0.60 -1.7 2,713,100 + -0.5 2,713,101 - +0.2 2,713,101 - -1.8 2,713,130 - +0.4 2,713,199 + Pf6N2E2_2293 0.11 +1.5 2,713,199 + Pf6N2E2_2293 0.11 -0.4 2,713,200 - Pf6N2E2_2293 0.11 -2.2 2,713,200 - Pf6N2E2_2293 0.11 -1.0 2,713,200 - Pf6N2E2_2293 0.11 +0.4 2,713,200 - Pf6N2E2_2293 0.11 -0.0 2,713,647 - Pf6N2E2_2293 0.34 +0.3 2,713,757 + Pf6N2E2_2293 0.40 -2.7
Or see this region's nucleotide sequence