Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2199 and Pf6N2E2_2200 are separated by 168 nucleotides Pf6N2E2_2200 and Pf6N2E2_2201 overlap by 4 nucleotides Pf6N2E2_2201 and Pf6N2E2_2202 are separated by 24 nucleotides Pf6N2E2_2202 and Pf6N2E2_2203 are separated by 327 nucleotides
Pf6N2E2_2199 - Catalase (EC 1.11.1.6), at 2,605,658 to 2,606,740
_2199
Pf6N2E2_2200 - RNA polymerase sigma-54 factor RpoN, at 2,606,909 to 2,607,415
_2200
Pf6N2E2_2201 - Transmembrane regulator protein PrtR, at 2,607,412 to 2,608,164
_2201
Pf6N2E2_2202 - Transcriptional regulator, IclR family, at 2,608,189 to 2,608,494
_2202
Pf6N2E2_2203 - Mobile element protein, at 2,608,822 to 2,610,090
_2203
Position (kb)
2607
2608
2609 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2606.503 kb on - strand, within Pf6N2E2_2199 at 2606.503 kb on - strand, within Pf6N2E2_2199 at 2606.629 kb on - strand, within Pf6N2E2_2199 at 2606.806 kb on + strand at 2606.822 kb on - strand at 2606.844 kb on + strand at 2606.908 kb on + strand at 2606.909 kb on - strand at 2607.131 kb on + strand, within Pf6N2E2_2200 at 2607.327 kb on + strand, within Pf6N2E2_2200 at 2607.451 kb on + strand at 2607.451 kb on + strand at 2607.451 kb on + strand at 2607.451 kb on + strand at 2607.452 kb on - strand at 2607.452 kb on - strand at 2607.452 kb on - strand at 2607.499 kb on + strand, within Pf6N2E2_2201 at 2607.499 kb on + strand, within Pf6N2E2_2201 at 2607.499 kb on + strand, within Pf6N2E2_2201 at 2607.500 kb on - strand, within Pf6N2E2_2201 at 2607.738 kb on - strand, within Pf6N2E2_2201 at 2607.863 kb on - strand, within Pf6N2E2_2201 at 2608.000 kb on + strand, within Pf6N2E2_2201 at 2608.000 kb on + strand, within Pf6N2E2_2201 at 2608.001 kb on - strand, within Pf6N2E2_2201 at 2608.075 kb on + strand, within Pf6N2E2_2201 at 2608.093 kb on + strand at 2608.094 kb on - strand at 2608.094 kb on - strand at 2608.094 kb on - strand at 2608.099 kb on + strand at 2608.099 kb on + strand at 2608.099 kb on + strand at 2608.099 kb on + strand at 2608.099 kb on + strand at 2608.099 kb on + strand at 2608.100 kb on - strand at 2608.100 kb on - strand at 2608.100 kb on - strand at 2608.100 kb on - strand at 2608.100 kb on - strand at 2608.100 kb on - strand at 2608.100 kb on - strand at 2608.100 kb on - strand at 2608.143 kb on + strand at 2608.144 kb on - strand at 2608.144 kb on - strand at 2608.489 kb on + strand at 2608.490 kb on - strand at 2608.490 kb on - strand at 2608.490 kb on - strand at 2608.490 kb on - strand at 2608.490 kb on - strand at 2608.511 kb on + strand at 2608.524 kb on + strand at 2608.566 kb on - strand at 2608.566 kb on - strand at 2608.566 kb on - strand at 2608.566 kb on - strand at 2608.566 kb on - strand at 2608.566 kb on - strand at 2608.566 kb on - strand at 2608.566 kb on - strand at 2608.566 kb on - strand at 2608.566 kb on - strand at 2608.566 kb on - strand at 2608.566 kb on - strand at 2608.570 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 2,606,503 - Pf6N2E2_2199 0.78 -0.4 2,606,503 - Pf6N2E2_2199 0.78 -0.5 2,606,629 - Pf6N2E2_2199 0.90 -0.1 2,606,806 + -3.0 2,606,822 - +0.5 2,606,844 + -0.8 2,606,908 + +0.0 2,606,909 - +0.3 2,607,131 + Pf6N2E2_2200 0.44 -0.2 2,607,327 + Pf6N2E2_2200 0.82 +1.5 2,607,451 + -1.3 2,607,451 + -3.3 2,607,451 + -1.1 2,607,451 + -3.1 2,607,452 - -1.1 2,607,452 - +1.5 2,607,452 - -1.8 2,607,499 + Pf6N2E2_2201 0.12 -2.5 2,607,499 + Pf6N2E2_2201 0.12 -1.9 2,607,499 + Pf6N2E2_2201 0.12 -2.3 2,607,500 - Pf6N2E2_2201 0.12 -1.1 2,607,738 - Pf6N2E2_2201 0.43 -0.5 2,607,863 - Pf6N2E2_2201 0.60 -2.2 2,608,000 + Pf6N2E2_2201 0.78 -0.6 2,608,000 + Pf6N2E2_2201 0.78 +0.9 2,608,001 - Pf6N2E2_2201 0.78 +1.5 2,608,075 + Pf6N2E2_2201 0.88 -0.1 2,608,093 + +0.3 2,608,094 - -1.3 2,608,094 - -0.3 2,608,094 - +0.5 2,608,099 + -0.1 2,608,099 + -0.0 2,608,099 + -0.4 2,608,099 + +0.3 2,608,099 + -1.1 2,608,099 + +1.5 2,608,100 - -0.4 2,608,100 - +0.1 2,608,100 - +0.2 2,608,100 - -0.2 2,608,100 - +0.2 2,608,100 - -2.5 2,608,100 - +0.4 2,608,100 - +0.4 2,608,143 + +0.0 2,608,144 - -1.1 2,608,144 - -0.6 2,608,489 + -1.2 2,608,490 - +1.1 2,608,490 - +1.5 2,608,490 - -0.0 2,608,490 - -1.5 2,608,490 - -1.1 2,608,511 + +0.4 2,608,524 + -0.1 2,608,566 - -0.2 2,608,566 - +0.1 2,608,566 - +0.1 2,608,566 - +0.3 2,608,566 - +0.5 2,608,566 - +0.1 2,608,566 - +0.2 2,608,566 - -0.2 2,608,566 - -0.2 2,608,566 - -0.2 2,608,566 - +0.8 2,608,566 - -0.1 2,608,570 - -0.1
Or see this region's nucleotide sequence