Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2181

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
2,585,388	-		Pf6N2E2_2180	0.24	-1.3
2,585,402	+		Pf6N2E2_2180	0.26	-3.5
2,585,781	-		Pf6N2E2_2180	0.63	-0.7
2,585,781	-		Pf6N2E2_2180	0.63	-3.0
2,585,781	-		Pf6N2E2_2180	0.63	-1.7
2,585,781	-		Pf6N2E2_2180	0.63	-0.7
2,585,888	+		Pf6N2E2_2180	0.73	-1.0
2,585,888	+				+1.5
2,585,889	-		Pf6N2E2_2180	0.74	-3.4
2,585,889	-		Pf6N2E2_2180	0.74	-0.5
2,585,896	+				-0.8
2,586,038	+		Pf6N2E2_2180	0.88	-2.6
2,586,153	+				-1.7
2,586,201	+				-0.2
2,586,201	+				+0.7
2,586,201	+				+0.1
2,586,201	+				-0.1
2,586,202	-				-0.6
2,586,202	-				-1.4
2,586,202	-				-1.9
2,586,202	-				-2.0
2,586,202	-				-0.5
2,586,202	-				-0.9
2,586,472	-				+1.5
2,586,472	-		Pf6N2E2_2181	0.24	-2.5
2,586,477	+		Pf6N2E2_2181	0.25	-2.6
2,586,850	-		Pf6N2E2_2181	0.61	-2.0
2,587,285	-				+0.3
2,587,285	-				-1.4
2,587,289	-				-0.4
2,587,289	-				+0.4
2,587,289	-				+0.6
2,587,329	-				-0.7
2,587,329	-				-0.6
2,587,357	-				-0.1
2,587,434	+				-0.5
2,587,457	+				+0.8
2,587,652	-		Pf6N2E2_2182	0.34	+1.5
2,587,671	+		Pf6N2E2_2182	0.36	+1.5
2,587,712	-		Pf6N2E2_2182	0.42	-0.2
2,587,884	-		Pf6N2E2_2182	0.64	+0.0
2,587,884	-		Pf6N2E2_2182	0.64	-0.9
2,587,884	-		Pf6N2E2_2182	0.64	+0.0
2,588,017	+		Pf6N2E2_2182	0.81	+1.4
2,588,017	+		Pf6N2E2_2182	0.81	+3.1
2,588,018	-		Pf6N2E2_2182	0.81	+0.1
2,588,024	-		Pf6N2E2_2182	0.82	+0.1
2,588,092	+				+1.5
2,588,093	-				+0.8
2,588,093	-				+0.3
2,588,199	+				+0.8
2,588,199	+				+1.5
2,588,200	-				+1.5
2,588,200	-				-0.6