Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_207

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_206 and Pf6N2E2_207 are separated by 12 nucleotidesPf6N2E2_207 and Pf6N2E2_208 are separated by 259 nucleotides Pf6N2E2_206 - Glycosyl transferase, group 2 family protein, at 257,106 to 258,128 _206 Pf6N2E2_207 - related to dolichyl-phosphate mannose synthase, at 258,141 to 260,522 _207 Pf6N2E2_208 - Glycosyl transferase, group 2 family protein, at 260,782 to 263,229 _208 Position (kb) 258 259 260 261Strain fitness (log2 ratio) -2 -1 0 1 2at 257.148 kb on - strandat 257.305 kb on + strand, within Pf6N2E2_206at 257.305 kb on + strand, within Pf6N2E2_206at 257.306 kb on - strand, within Pf6N2E2_206at 257.306 kb on - strand, within Pf6N2E2_206at 257.306 kb on - strand, within Pf6N2E2_206at 257.387 kb on - strand, within Pf6N2E2_206at 257.389 kb on + strand, within Pf6N2E2_206at 257.390 kb on - strand, within Pf6N2E2_206at 257.395 kb on + strand, within Pf6N2E2_206at 257.395 kb on + strand, within Pf6N2E2_206at 257.395 kb on + strand, within Pf6N2E2_206at 257.396 kb on - strand, within Pf6N2E2_206at 257.459 kb on + strand, within Pf6N2E2_206at 257.459 kb on + strand, within Pf6N2E2_206at 257.761 kb on + strand, within Pf6N2E2_206at 257.761 kb on + strand, within Pf6N2E2_206at 257.850 kb on - strand, within Pf6N2E2_206at 258.004 kb on + strand, within Pf6N2E2_206at 258.004 kb on + strand, within Pf6N2E2_206at 258.004 kb on + strand, within Pf6N2E2_206at 258.004 kb on + strand, within Pf6N2E2_206at 258.004 kb on + strand, within Pf6N2E2_206at 258.004 kb on + strand, within Pf6N2E2_206at 258.005 kb on - strand, within Pf6N2E2_206at 258.005 kb on - strand, within Pf6N2E2_206at 258.005 kb on - strand, within Pf6N2E2_206at 258.005 kb on - strand, within Pf6N2E2_206at 258.005 kb on - strand, within Pf6N2E2_206at 258.028 kb on - strandat 258.048 kb on + strandat 258.048 kb on + strandat 258.049 kb on - strandat 258.050 kb on + strandat 258.051 kb on - strandat 258.051 kb on - strandat 258.051 kb on - strandat 258.199 kb on + strandat 258.199 kb on + strandat 258.199 kb on + strandat 258.199 kb on + strandat 258.199 kb on + strandat 258.200 kb on - strandat 258.200 kb on - strandat 258.383 kb on - strand, within Pf6N2E2_207at 258.383 kb on - strand, within Pf6N2E2_207at 258.463 kb on + strand, within Pf6N2E2_207at 258.464 kb on - strand, within Pf6N2E2_207at 258.577 kb on + strand, within Pf6N2E2_207at 258.578 kb on - strand, within Pf6N2E2_207at 258.730 kb on + strand, within Pf6N2E2_207at 258.861 kb on + strand, within Pf6N2E2_207at 258.862 kb on - strand, within Pf6N2E2_207at 258.862 kb on - strand, within Pf6N2E2_207at 258.862 kb on - strand, within Pf6N2E2_207at 258.862 kb on - strand, within Pf6N2E2_207at 258.862 kb on - strand, within Pf6N2E2_207at 258.865 kb on + strand, within Pf6N2E2_207at 258.865 kb on + strand, within Pf6N2E2_207at 258.865 kb on + strand, within Pf6N2E2_207at 258.865 kb on + strand, within Pf6N2E2_207at 258.865 kb on + strand, within Pf6N2E2_207at 258.866 kb on - strand, within Pf6N2E2_207at 258.916 kb on + strand, within Pf6N2E2_207at 258.919 kb on + strand, within Pf6N2E2_207at 258.920 kb on - strand, within Pf6N2E2_207at 258.920 kb on - strand, within Pf6N2E2_207at 259.060 kb on + strand, within Pf6N2E2_207at 259.060 kb on + strand, within Pf6N2E2_207at 259.061 kb on - strand, within Pf6N2E2_207at 259.381 kb on + strand, within Pf6N2E2_207at 259.462 kb on + strand, within Pf6N2E2_207at 259.462 kb on + strand, within Pf6N2E2_207at 259.462 kb on + strand, within Pf6N2E2_207at 259.462 kb on + strand, within Pf6N2E2_207at 259.829 kb on + strand, within Pf6N2E2_207at 259.829 kb on + strand, within Pf6N2E2_207at 259.829 kb on + strand, within Pf6N2E2_207at 259.829 kb on + strand, within Pf6N2E2_207at 259.830 kb on - strand, within Pf6N2E2_207at 259.830 kb on - strand, within Pf6N2E2_207at 259.830 kb on - strand, within Pf6N2E2_207at 259.831 kb on + strand, within Pf6N2E2_207at 259.831 kb on + strand, within Pf6N2E2_207at 259.831 kb on + strand, within Pf6N2E2_207at 259.832 kb on - strand, within Pf6N2E2_207at 259.832 kb on - strand, within Pf6N2E2_207at 259.832 kb on - strand, within Pf6N2E2_207at 259.832 kb on - strand, within Pf6N2E2_207at 259.832 kb on - strand, within Pf6N2E2_207at 259.832 kb on - strand, within Pf6N2E2_207at 259.844 kb on - strand, within Pf6N2E2_207at 259.844 kb on - strand, within Pf6N2E2_207at 259.882 kb on + strand, within Pf6N2E2_207at 259.882 kb on + strand, within Pf6N2E2_207at 259.882 kb on + strand, within Pf6N2E2_207at 259.882 kb on + strand, within Pf6N2E2_207at 259.882 kb on + strand, within Pf6N2E2_207at 259.882 kb on + strand, within Pf6N2E2_207at 259.882 kb on + strand, within Pf6N2E2_207at 259.882 kb on + strand, within Pf6N2E2_207at 259.882 kb on + strand, within Pf6N2E2_207at 259.882 kb on + strand, within Pf6N2E2_207at 259.883 kb on - strand, within Pf6N2E2_207at 259.883 kb on - strand, within Pf6N2E2_207at 259.883 kb on - strand, within Pf6N2E2_207at 259.883 kb on - strand, within Pf6N2E2_207at 259.883 kb on - strand, within Pf6N2E2_207at 259.883 kb on - strand, within Pf6N2E2_207at 259.883 kb on - strand, within Pf6N2E2_207at 259.993 kb on + strand, within Pf6N2E2_207at 259.993 kb on + strand, within Pf6N2E2_207at 259.993 kb on + strand, within Pf6N2E2_207at 259.994 kb on - strand, within Pf6N2E2_207at 259.994 kb on - strand, within Pf6N2E2_207at 260.146 kb on + strand, within Pf6N2E2_207at 260.147 kb on - strand, within Pf6N2E2_207at 260.158 kb on + strand, within Pf6N2E2_207at 260.173 kb on + strand, within Pf6N2E2_207at 260.174 kb on - strand, within Pf6N2E2_207at 260.427 kb on + strandat 260.479 kb on + strandat 260.480 kb on - strandat 260.527 kb on + strandat 260.527 kb on + strandat 260.553 kb on - strandat 260.586 kb on - strandat 260.754 kb on + strandat 260.783 kb on + strandat 260.783 kb on + strandat 260.784 kb on - strandat 260.784 kb on - strandat 260.784 kb on - strandat 260.785 kb on + strandat 260.785 kb on + strandat 260.785 kb on + strandat 260.785 kb on + strandat 260.786 kb on - strandat 260.786 kb on - strandat 260.786 kb on - strandat 260.786 kb on - strandat 260.796 kb on - strandat 260.970 kb on - strandat 260.999 kb on + strandat 261.000 kb on - strandat 261.000 kb on - strandat 261.000 kb on - strandat 261.000 kb on - strandat 261.146 kb on + strand, within Pf6N2E2_208at 261.291 kb on - strand, within Pf6N2E2_208at 261.515 kb on + strand, within Pf6N2E2_208at 261.515 kb on + strand, within Pf6N2E2_208at 261.515 kb on + strand, within Pf6N2E2_208at 261.516 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
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257,148 - -1.0
257,305 + Pf6N2E2_206 0.19 -2.4
257,305 + Pf6N2E2_206 0.19 +0.0
257,306 - Pf6N2E2_206 0.20 +0.2
257,306 - Pf6N2E2_206 0.20 +0.5
257,306 - Pf6N2E2_206 0.20 +1.1
257,387 - Pf6N2E2_206 0.27 -0.1
257,389 + Pf6N2E2_206 0.28 +0.3
257,390 - Pf6N2E2_206 0.28 +2.5
257,395 + Pf6N2E2_206 0.28 +0.9
257,395 + Pf6N2E2_206 0.28 +0.2
257,395 + Pf6N2E2_206 0.28 +0.7
257,396 - Pf6N2E2_206 0.28 +0.0
257,459 + Pf6N2E2_206 0.35 +0.5
257,459 + Pf6N2E2_206 0.35 +0.3
257,761 + Pf6N2E2_206 0.64 -0.0
257,761 + Pf6N2E2_206 0.64 +1.1
257,850 - Pf6N2E2_206 0.73 -0.6
258,004 + Pf6N2E2_206 0.88 +0.0
258,004 + Pf6N2E2_206 0.88 +0.5
258,004 + Pf6N2E2_206 0.88 +0.1
258,004 + Pf6N2E2_206 0.88 +1.5
258,004 + Pf6N2E2_206 0.88 -0.1
258,004 + Pf6N2E2_206 0.88 +0.8
258,005 - Pf6N2E2_206 0.88 +1.5
258,005 - Pf6N2E2_206 0.88 -0.4
258,005 - Pf6N2E2_206 0.88 +0.3
258,005 - Pf6N2E2_206 0.88 -0.9
258,005 - Pf6N2E2_206 0.88 -2.1
258,028 - -0.5
258,048 + +1.0
258,048 + +0.4
258,049 - +0.1
258,050 + -0.9
258,051 - -1.0
258,051 - +0.8
258,051 - +1.5
258,199 + +0.6
258,199 + +0.2
258,199 + +0.2
258,199 + +0.7
258,199 + -0.5
258,200 - -0.5
258,200 - +0.2
258,383 - Pf6N2E2_207 0.10 -0.0
258,383 - Pf6N2E2_207 0.10 -1.3
258,463 + Pf6N2E2_207 0.14 +0.4
258,464 - Pf6N2E2_207 0.14 -0.2
258,577 + Pf6N2E2_207 0.18 +1.3
258,578 - Pf6N2E2_207 0.18 -0.8
258,730 + Pf6N2E2_207 0.25 +0.4
258,861 + Pf6N2E2_207 0.30 +1.5
258,862 - Pf6N2E2_207 0.30 -0.6
258,862 - Pf6N2E2_207 0.30 +0.5
258,862 - Pf6N2E2_207 0.30 +0.4
258,862 - Pf6N2E2_207 0.30 -0.3
258,862 - Pf6N2E2_207 0.30 +2.5
258,865 + Pf6N2E2_207 0.30 -0.5
258,865 + Pf6N2E2_207 0.30 +1.2
258,865 + Pf6N2E2_207 0.30 +2.5
258,865 + Pf6N2E2_207 0.30 -0.5
258,865 + Pf6N2E2_207 0.30 +0.5
258,866 - Pf6N2E2_207 0.30 +0.2
258,916 + Pf6N2E2_207 0.33 +1.0
258,919 + Pf6N2E2_207 0.33 -0.1
258,920 - Pf6N2E2_207 0.33 +1.5
258,920 - Pf6N2E2_207 0.33 +0.8
259,060 + Pf6N2E2_207 0.39 +0.5
259,060 + Pf6N2E2_207 0.39 -0.5
259,061 - Pf6N2E2_207 0.39 -2.2
259,381 + Pf6N2E2_207 0.52 +0.2
259,462 + Pf6N2E2_207 0.55 -0.3
259,462 + Pf6N2E2_207 0.55 +0.4
259,462 + Pf6N2E2_207 0.55 +0.9
259,462 + Pf6N2E2_207 0.55 -1.4
259,829 + Pf6N2E2_207 0.71 -0.2
259,829 + Pf6N2E2_207 0.71 +0.7
259,829 + Pf6N2E2_207 0.71 -0.0
259,829 + Pf6N2E2_207 0.71 +1.5
259,830 - Pf6N2E2_207 0.71 +0.6
259,830 - Pf6N2E2_207 0.71 -0.5
259,830 - Pf6N2E2_207 0.71 -1.3
259,831 + Pf6N2E2_207 0.71 +0.0
259,831 + Pf6N2E2_207 0.71 -0.3
259,831 + Pf6N2E2_207 0.71 -0.9
259,832 - Pf6N2E2_207 0.71 -0.0
259,832 - Pf6N2E2_207 0.71 +0.3
259,832 - Pf6N2E2_207 0.71 +0.2
259,832 - Pf6N2E2_207 0.71 +0.9
259,832 - Pf6N2E2_207 0.71 +0.2
259,832 - Pf6N2E2_207 0.71 +0.4
259,844 - Pf6N2E2_207 0.71 +1.5
259,844 - Pf6N2E2_207 0.71 -0.7
259,882 + Pf6N2E2_207 0.73 -0.0
259,882 + Pf6N2E2_207 0.73 -0.8
259,882 + Pf6N2E2_207 0.73 -0.8
259,882 + Pf6N2E2_207 0.73 +1.2
259,882 + Pf6N2E2_207 0.73 -0.2
259,882 + Pf6N2E2_207 0.73 +0.4
259,882 + Pf6N2E2_207 0.73 -0.3
259,882 + Pf6N2E2_207 0.73 +0.0
259,882 + Pf6N2E2_207 0.73 +0.5
259,882 + Pf6N2E2_207 0.73 +0.5
259,883 - Pf6N2E2_207 0.73 +1.0
259,883 - Pf6N2E2_207 0.73 -1.7
259,883 - Pf6N2E2_207 0.73 -1.5
259,883 - Pf6N2E2_207 0.73 -0.5
259,883 - Pf6N2E2_207 0.73 +0.2
259,883 - Pf6N2E2_207 0.73 +0.1
259,883 - Pf6N2E2_207 0.73 +1.0
259,993 + Pf6N2E2_207 0.78 +0.2
259,993 + Pf6N2E2_207 0.78 +0.1
259,993 + Pf6N2E2_207 0.78 +0.6
259,994 - Pf6N2E2_207 0.78 +0.3
259,994 - Pf6N2E2_207 0.78 +0.3
260,146 + Pf6N2E2_207 0.84 +0.2
260,147 - Pf6N2E2_207 0.84 -0.5
260,158 + Pf6N2E2_207 0.85 +0.3
260,173 + Pf6N2E2_207 0.85 +0.3
260,174 - Pf6N2E2_207 0.85 +0.4
260,427 + -0.2
260,479 + +0.4
260,480 - -0.4
260,527 + -2.3
260,527 + +0.1
260,553 - -0.0
260,586 - +1.5
260,754 + +0.2
260,783 + -1.3
260,783 + -0.3
260,784 - -0.9
260,784 - +1.4
260,784 - -1.4
260,785 + +1.0
260,785 + -0.7
260,785 + -0.6
260,785 + +1.5
260,786 - +0.1
260,786 - +0.1
260,786 - -0.5
260,786 - -0.0
260,796 - -0.2
260,970 - +0.5
260,999 + -0.7
261,000 - +0.5
261,000 - +1.5
261,000 - -0.3
261,000 - -0.6
261,146 + Pf6N2E2_208 0.15 +0.5
261,291 - Pf6N2E2_208 0.21 +0.4
261,515 + Pf6N2E2_208 0.30 -0.2
261,515 + Pf6N2E2_208 0.30 +0.2
261,515 + Pf6N2E2_208 0.30 +0.4
261,516 - -0.5

Or see this region's nucleotide sequence