Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2059

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2057 and Pf6N2E2_2058 are separated by 53 nucleotidesPf6N2E2_2058 and Pf6N2E2_2059 are separated by 53 nucleotidesPf6N2E2_2059 and Pf6N2E2_2060 are separated by 174 nucleotides Pf6N2E2_2057 - Phosphatidylserine/phosphatidylglycerophosphate/ cardiolipi n synthases and related enzymes, at 2,445,939 to 2,447,585 _2057 Pf6N2E2_2058 - ChlD component of cobalt chelatase involved in B12 biosynthesis, at 2,447,639 to 2,448,250 _2058 Pf6N2E2_2059 - ChlI component of cobalt chelatase involved in B12 biosynthesis, at 2,448,304 to 2,449,308 _2059 Pf6N2E2_2060 - CobN component of cobalt chelatase involved in B12 biosynthesis, at 2,449,483 to 2,453,256 _2060 Position (kb) 2448 2449 2450Strain fitness (log2 ratio) -2 -1 0 1 2at 2447.323 kb on + strand, within Pf6N2E2_2057at 2447.323 kb on + strand, within Pf6N2E2_2057at 2447.323 kb on + strand, within Pf6N2E2_2057at 2447.517 kb on + strandat 2447.517 kb on + strandat 2447.537 kb on - strandat 2447.577 kb on + strandat 2447.616 kb on + strandat 2447.616 kb on + strandat 2447.617 kb on - strandat 2447.617 kb on - strandat 2447.617 kb on - strandat 2447.617 kb on - strandat 2447.635 kb on + strandat 2447.635 kb on + strandat 2447.635 kb on + strandat 2447.636 kb on - strandat 2447.636 kb on - strandat 2447.662 kb on + strandat 2447.663 kb on - strandat 2447.663 kb on - strandat 2447.671 kb on - strandat 2448.306 kb on - strandat 2449.001 kb on + strand, within Pf6N2E2_2059at 2449.001 kb on + strand, within Pf6N2E2_2059at 2449.002 kb on - strand, within Pf6N2E2_2059at 2449.002 kb on - strand, within Pf6N2E2_2059at 2449.652 kb on + strandat 2449.653 kb on - strandat 2449.653 kb on - strandat 2449.673 kb on + strandat 2449.673 kb on + strandat 2449.757 kb on + strandat 2449.758 kb on - strandat 2449.880 kb on + strand, within Pf6N2E2_2060at 2449.880 kb on + strand, within Pf6N2E2_2060at 2449.881 kb on - strand, within Pf6N2E2_2060at 2449.881 kb on - strand, within Pf6N2E2_2060at 2449.943 kb on + strand, within Pf6N2E2_2060at 2449.943 kb on + strand, within Pf6N2E2_2060at 2449.943 kb on + strand, within Pf6N2E2_2060at 2449.943 kb on + strand, within Pf6N2E2_2060at 2449.943 kb on + strand, within Pf6N2E2_2060at 2449.944 kb on - strand, within Pf6N2E2_2060at 2449.944 kb on - strand, within Pf6N2E2_2060at 2449.946 kb on + strand, within Pf6N2E2_2060at 2449.947 kb on - strand, within Pf6N2E2_2060

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
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2,447,323 + Pf6N2E2_2057 0.84 -0.2
2,447,323 + Pf6N2E2_2057 0.84 -0.2
2,447,323 + Pf6N2E2_2057 0.84 +0.8
2,447,517 + +0.1
2,447,517 + +1.2
2,447,537 - +1.6
2,447,577 + -1.4
2,447,616 + +0.4
2,447,616 + -0.9
2,447,617 - -0.2
2,447,617 - +0.9
2,447,617 - +0.5
2,447,617 - -0.2
2,447,635 + +1.6
2,447,635 + +1.6
2,447,635 + +0.4
2,447,636 - -0.2
2,447,636 - +0.4
2,447,662 + -1.4
2,447,663 - -1.3
2,447,663 - +1.6
2,447,671 - +0.6
2,448,306 - +2.0
2,449,001 + Pf6N2E2_2059 0.69 -1.2
2,449,001 + Pf6N2E2_2059 0.69 -0.7
2,449,002 - Pf6N2E2_2059 0.69 +0.6
2,449,002 - Pf6N2E2_2059 0.69 +1.6
2,449,652 + -0.7
2,449,653 - -1.4
2,449,653 - +0.6
2,449,673 + -0.6
2,449,673 + +0.6
2,449,757 + -0.1
2,449,758 - +1.2
2,449,880 + Pf6N2E2_2060 0.11 +0.4
2,449,880 + Pf6N2E2_2060 0.11 +1.6
2,449,881 - Pf6N2E2_2060 0.11 -0.6
2,449,881 - Pf6N2E2_2060 0.11 -0.6
2,449,943 + Pf6N2E2_2060 0.12 +0.4
2,449,943 + Pf6N2E2_2060 0.12 +0.0
2,449,943 + Pf6N2E2_2060 0.12 +0.3
2,449,943 + Pf6N2E2_2060 0.12 +0.2
2,449,943 + Pf6N2E2_2060 0.12 -0.1
2,449,944 - Pf6N2E2_2060 0.12 -2.0
2,449,944 - Pf6N2E2_2060 0.12 -1.9
2,449,946 + Pf6N2E2_2060 0.12 -0.4
2,449,947 - Pf6N2E2_2060 0.12 +1.6

Or see this region's nucleotide sequence