Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1918 and Pf6N2E2_1919 are separated by 205 nucleotides Pf6N2E2_1919 and Pf6N2E2_1920 are separated by 87 nucleotides Pf6N2E2_1920 and Pf6N2E2_1921 are separated by 22 nucleotides Pf6N2E2_1921 and Pf6N2E2_1922 overlap by 4 nucleotides
Pf6N2E2_1918 - Major facilitator family transporter, at 2,271,740 to 2,272,900
_1918
Pf6N2E2_1919 - Aldehyde dehydrogenase (EC 1.2.1.3), at 2,273,106 to 2,274,578
_1919
Pf6N2E2_1920 - Rubredoxin, at 2,274,666 to 2,274,872
_1920
Pf6N2E2_1921 - hypothetical protein, at 2,274,895 to 2,275,032
_1921
Pf6N2E2_1922 - Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 2,275,029 to 2,277,140
_1922
Position (kb)
2273
2274
2275 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2272.128 kb on - strand, within Pf6N2E2_1918 at 2272.216 kb on - strand, within Pf6N2E2_1918 at 2272.216 kb on - strand, within Pf6N2E2_1918 at 2272.216 kb on - strand, within Pf6N2E2_1918 at 2272.221 kb on + strand, within Pf6N2E2_1918 at 2272.222 kb on - strand, within Pf6N2E2_1918 at 2272.222 kb on - strand, within Pf6N2E2_1918 at 2272.222 kb on - strand, within Pf6N2E2_1918 at 2272.222 kb on - strand, within Pf6N2E2_1918 at 2272.231 kb on - strand, within Pf6N2E2_1918 at 2272.398 kb on + strand, within Pf6N2E2_1918 at 2272.436 kb on - strand at 2272.605 kb on + strand, within Pf6N2E2_1918 at 2272.628 kb on - strand, within Pf6N2E2_1918 at 2272.665 kb on + strand, within Pf6N2E2_1918 at 2272.665 kb on + strand, within Pf6N2E2_1918 at 2272.665 kb on + strand, within Pf6N2E2_1918 at 2272.665 kb on + strand, within Pf6N2E2_1918 at 2272.665 kb on + strand, within Pf6N2E2_1918 at 2272.666 kb on - strand, within Pf6N2E2_1918 at 2272.666 kb on - strand, within Pf6N2E2_1918 at 2272.666 kb on - strand, within Pf6N2E2_1918 at 2272.666 kb on - strand, within Pf6N2E2_1918 at 2272.737 kb on + strand, within Pf6N2E2_1918 at 2272.737 kb on + strand, within Pf6N2E2_1918 at 2272.738 kb on - strand, within Pf6N2E2_1918 at 2272.738 kb on - strand, within Pf6N2E2_1918 at 2272.738 kb on - strand, within Pf6N2E2_1918 at 2272.738 kb on - strand, within Pf6N2E2_1918 at 2272.738 kb on - strand, within Pf6N2E2_1918 at 2272.738 kb on - strand, within Pf6N2E2_1918 at 2272.738 kb on - strand, within Pf6N2E2_1918 at 2272.847 kb on - strand at 2272.973 kb on + strand at 2272.973 kb on + strand at 2273.018 kb on + strand at 2273.019 kb on - strand at 2273.127 kb on + strand at 2273.149 kb on + strand at 2273.149 kb on + strand at 2273.149 kb on + strand at 2273.150 kb on - strand at 2273.334 kb on + strand, within Pf6N2E2_1919 at 2273.431 kb on - strand, within Pf6N2E2_1919 at 2273.431 kb on - strand, within Pf6N2E2_1919 at 2273.431 kb on - strand, within Pf6N2E2_1919 at 2273.482 kb on - strand, within Pf6N2E2_1919 at 2273.482 kb on - strand, within Pf6N2E2_1919 at 2273.617 kb on - strand, within Pf6N2E2_1919 at 2273.659 kb on + strand, within Pf6N2E2_1919 at 2273.660 kb on - strand, within Pf6N2E2_1919 at 2273.664 kb on - strand, within Pf6N2E2_1919 at 2273.664 kb on - strand, within Pf6N2E2_1919 at 2273.995 kb on - strand, within Pf6N2E2_1919 at 2274.259 kb on - strand, within Pf6N2E2_1919 at 2274.259 kb on - strand, within Pf6N2E2_1919 at 2274.341 kb on + strand, within Pf6N2E2_1919 at 2274.341 kb on + strand, within Pf6N2E2_1919 at 2274.341 kb on + strand, within Pf6N2E2_1919 at 2274.342 kb on - strand, within Pf6N2E2_1919 at 2274.344 kb on + strand, within Pf6N2E2_1919 at 2274.345 kb on - strand, within Pf6N2E2_1919 at 2274.345 kb on - strand, within Pf6N2E2_1919 at 2274.345 kb on - strand, within Pf6N2E2_1919 at 2274.557 kb on + strand at 2274.558 kb on - strand at 2274.558 kb on - strand at 2274.558 kb on - strand at 2274.664 kb on - strand at 2274.664 kb on - strand at 2274.760 kb on - strand, within Pf6N2E2_1920 at 2274.760 kb on - strand, within Pf6N2E2_1920 at 2274.777 kb on - strand, within Pf6N2E2_1920 at 2274.777 kb on - strand, within Pf6N2E2_1920 at 2274.896 kb on + strand at 2274.935 kb on + strand, within Pf6N2E2_1921 at 2275.033 kb on - strand at 2275.033 kb on - strand at 2275.033 kb on - strand at 2275.094 kb on + strand at 2275.115 kb on - strand at 2275.178 kb on - strand at 2275.216 kb on + strand at 2275.257 kb on - strand, within Pf6N2E2_1922 at 2275.257 kb on - strand, within Pf6N2E2_1922 at 2275.257 kb on - strand, within Pf6N2E2_1922 at 2275.299 kb on + strand, within Pf6N2E2_1922 at 2275.299 kb on + strand, within Pf6N2E2_1922 at 2275.335 kb on - strand, within Pf6N2E2_1922 at 2275.430 kb on - strand, within Pf6N2E2_1922 at 2275.501 kb on + strand, within Pf6N2E2_1922 at 2275.502 kb on - strand, within Pf6N2E2_1922 at 2275.541 kb on - strand, within Pf6N2E2_1922
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 2,272,128 - Pf6N2E2_1918 0.33 -0.1 2,272,216 - Pf6N2E2_1918 0.41 -0.1 2,272,216 - Pf6N2E2_1918 0.41 -0.4 2,272,216 - Pf6N2E2_1918 0.41 -0.3 2,272,221 + Pf6N2E2_1918 0.41 -0.7 2,272,222 - Pf6N2E2_1918 0.42 -0.2 2,272,222 - Pf6N2E2_1918 0.42 -0.6 2,272,222 - Pf6N2E2_1918 0.42 +0.2 2,272,222 - Pf6N2E2_1918 0.42 -0.2 2,272,231 - Pf6N2E2_1918 0.42 +1.6 2,272,398 + Pf6N2E2_1918 0.57 +0.4 2,272,436 - -1.7 2,272,605 + Pf6N2E2_1918 0.75 -0.1 2,272,628 - Pf6N2E2_1918 0.76 -0.4 2,272,665 + Pf6N2E2_1918 0.80 +0.1 2,272,665 + Pf6N2E2_1918 0.80 -1.3 2,272,665 + Pf6N2E2_1918 0.80 +0.1 2,272,665 + Pf6N2E2_1918 0.80 -0.1 2,272,665 + Pf6N2E2_1918 0.80 -0.1 2,272,666 - Pf6N2E2_1918 0.80 +0.0 2,272,666 - Pf6N2E2_1918 0.80 -0.2 2,272,666 - Pf6N2E2_1918 0.80 -0.5 2,272,666 - Pf6N2E2_1918 0.80 -0.2 2,272,737 + Pf6N2E2_1918 0.86 +0.5 2,272,737 + Pf6N2E2_1918 0.86 +3.2 2,272,738 - Pf6N2E2_1918 0.86 -0.3 2,272,738 - Pf6N2E2_1918 0.86 -0.7 2,272,738 - Pf6N2E2_1918 0.86 +0.0 2,272,738 - Pf6N2E2_1918 0.86 +0.0 2,272,738 - Pf6N2E2_1918 0.86 -0.7 2,272,738 - Pf6N2E2_1918 0.86 -0.5 2,272,738 - Pf6N2E2_1918 0.86 +0.6 2,272,847 - -0.3 2,272,973 + +1.0 2,272,973 + +0.5 2,273,018 + -0.5 2,273,019 - -0.8 2,273,127 + +0.6 2,273,149 + +0.2 2,273,149 + -0.1 2,273,149 + +1.6 2,273,150 - +0.7 2,273,334 + Pf6N2E2_1919 0.15 -0.4 2,273,431 - Pf6N2E2_1919 0.22 +0.0 2,273,431 - Pf6N2E2_1919 0.22 -0.9 2,273,431 - Pf6N2E2_1919 0.22 +0.0 2,273,482 - Pf6N2E2_1919 0.26 -0.9 2,273,482 - Pf6N2E2_1919 0.26 -1.4 2,273,617 - Pf6N2E2_1919 0.35 +0.1 2,273,659 + Pf6N2E2_1919 0.38 -1.2 2,273,660 - Pf6N2E2_1919 0.38 -1.1 2,273,664 - Pf6N2E2_1919 0.38 -0.4 2,273,664 - Pf6N2E2_1919 0.38 -1.3 2,273,995 - Pf6N2E2_1919 0.60 +0.2 2,274,259 - Pf6N2E2_1919 0.78 +1.6 2,274,259 - Pf6N2E2_1919 0.78 +1.3 2,274,341 + Pf6N2E2_1919 0.84 -1.1 2,274,341 + Pf6N2E2_1919 0.84 +0.4 2,274,341 + Pf6N2E2_1919 0.84 -1.1 2,274,342 - Pf6N2E2_1919 0.84 +1.6 2,274,344 + Pf6N2E2_1919 0.84 +0.6 2,274,345 - Pf6N2E2_1919 0.84 -0.7 2,274,345 - Pf6N2E2_1919 0.84 +1.3 2,274,345 - Pf6N2E2_1919 0.84 -0.3 2,274,557 + +0.4 2,274,558 - -0.8 2,274,558 - -1.2 2,274,558 - -1.6 2,274,664 - +0.6 2,274,664 - +0.2 2,274,760 - Pf6N2E2_1920 0.45 +1.2 2,274,760 - Pf6N2E2_1920 0.45 +0.8 2,274,777 - Pf6N2E2_1920 0.54 -0.2 2,274,777 - Pf6N2E2_1920 0.54 -1.4 2,274,896 + +2.2 2,274,935 + Pf6N2E2_1921 0.29 +0.9 2,275,033 - -0.7 2,275,033 - -0.2 2,275,033 - -0.5 2,275,094 + -0.7 2,275,115 - -1.5 2,275,178 - +1.2 2,275,216 + +1.6 2,275,257 - Pf6N2E2_1922 0.11 +1.8 2,275,257 - Pf6N2E2_1922 0.11 +0.4 2,275,257 - Pf6N2E2_1922 0.11 -0.2 2,275,299 + Pf6N2E2_1922 0.13 +0.0 2,275,299 + Pf6N2E2_1922 0.13 +0.5 2,275,335 - Pf6N2E2_1922 0.14 +0.8 2,275,430 - Pf6N2E2_1922 0.19 +0.9 2,275,501 + Pf6N2E2_1922 0.22 -0.7 2,275,502 - Pf6N2E2_1922 0.22 -0.2 2,275,541 - Pf6N2E2_1922 0.24 +0.3
Or see this region's nucleotide sequence