Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1768 and Pf6N2E2_1769 overlap by 4 nucleotides Pf6N2E2_1769 and Pf6N2E2_1770 are separated by 44 nucleotides Pf6N2E2_1770 and Pf6N2E2_1771 are separated by 56 nucleotides
Pf6N2E2_1768 - O-acetylhomoserine sulfhydrylase (EC 2.5.1.49), at 2,089,558 to 2,090,982
_1768
Pf6N2E2_1769 - Nucleoside-diphosphate-sugar epimerases, at 2,090,979 to 2,091,320
_1769
Pf6N2E2_1770 - Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2), at 2,091,365 to 2,092,438
_1770
Pf6N2E2_1771 - Response regulator, at 2,092,495 to 2,093,475
_1771
Position (kb)
2091
2092
2093 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2090.425 kb on + strand, within Pf6N2E2_1768 at 2090.425 kb on + strand, within Pf6N2E2_1768 at 2090.426 kb on - strand, within Pf6N2E2_1768 at 2090.476 kb on + strand, within Pf6N2E2_1768 at 2090.696 kb on + strand, within Pf6N2E2_1768 at 2090.696 kb on + strand, within Pf6N2E2_1768 at 2090.843 kb on - strand at 2090.883 kb on - strand at 2090.883 kb on - strand at 2091.220 kb on + strand, within Pf6N2E2_1769 at 2091.220 kb on + strand, within Pf6N2E2_1769 at 2091.220 kb on + strand, within Pf6N2E2_1769 at 2091.220 kb on + strand, within Pf6N2E2_1769 at 2091.220 kb on + strand, within Pf6N2E2_1769 at 2091.220 kb on + strand, within Pf6N2E2_1769 at 2091.221 kb on - strand, within Pf6N2E2_1769 at 2091.221 kb on - strand, within Pf6N2E2_1769 at 2091.221 kb on - strand, within Pf6N2E2_1769 at 2091.221 kb on - strand, within Pf6N2E2_1769 at 2091.224 kb on + strand, within Pf6N2E2_1769 at 2091.224 kb on + strand, within Pf6N2E2_1769 at 2091.225 kb on - strand, within Pf6N2E2_1769 at 2091.225 kb on - strand, within Pf6N2E2_1769 at 2091.225 kb on - strand, within Pf6N2E2_1769 at 2091.225 kb on - strand, within Pf6N2E2_1769 at 2091.280 kb on + strand, within Pf6N2E2_1769 at 2091.303 kb on + strand at 2091.304 kb on - strand at 2091.304 kb on - strand at 2091.304 kb on - strand at 2091.308 kb on - strand at 2091.388 kb on - strand at 2091.434 kb on + strand at 2091.434 kb on + strand at 2091.434 kb on + strand at 2091.449 kb on + strand at 2091.507 kb on - strand, within Pf6N2E2_1770 at 2091.507 kb on - strand, within Pf6N2E2_1770 at 2091.729 kb on - strand, within Pf6N2E2_1770 at 2091.729 kb on - strand, within Pf6N2E2_1770 at 2091.792 kb on + strand, within Pf6N2E2_1770 at 2091.792 kb on + strand, within Pf6N2E2_1770 at 2091.792 kb on + strand, within Pf6N2E2_1770 at 2091.793 kb on - strand, within Pf6N2E2_1770 at 2091.793 kb on - strand, within Pf6N2E2_1770 at 2091.905 kb on + strand, within Pf6N2E2_1770 at 2091.906 kb on - strand, within Pf6N2E2_1770 at 2091.906 kb on - strand, within Pf6N2E2_1770 at 2091.906 kb on - strand, within Pf6N2E2_1770 at 2091.945 kb on - strand, within Pf6N2E2_1770 at 2091.983 kb on + strand, within Pf6N2E2_1770 at 2091.993 kb on - strand, within Pf6N2E2_1770 at 2091.993 kb on - strand, within Pf6N2E2_1770 at 2092.010 kb on + strand, within Pf6N2E2_1770 at 2092.010 kb on + strand, within Pf6N2E2_1770 at 2092.011 kb on - strand, within Pf6N2E2_1770 at 2092.122 kb on - strand, within Pf6N2E2_1770 at 2092.217 kb on + strand, within Pf6N2E2_1770 at 2092.217 kb on + strand, within Pf6N2E2_1770 at 2092.217 kb on + strand, within Pf6N2E2_1770 at 2092.217 kb on + strand, within Pf6N2E2_1770 at 2092.217 kb on + strand, within Pf6N2E2_1770 at 2092.218 kb on - strand, within Pf6N2E2_1770 at 2092.266 kb on - strand, within Pf6N2E2_1770 at 2092.331 kb on + strand at 2092.365 kb on + strand at 2092.423 kb on + strand at 2092.424 kb on - strand at 2092.532 kb on - strand at 2092.639 kb on - strand, within Pf6N2E2_1771 at 2092.641 kb on - strand, within Pf6N2E2_1771 at 2092.641 kb on - strand, within Pf6N2E2_1771 at 2092.657 kb on - strand, within Pf6N2E2_1771 at 2092.668 kb on - strand, within Pf6N2E2_1771 at 2092.668 kb on - strand, within Pf6N2E2_1771 at 2092.668 kb on - strand, within Pf6N2E2_1771 at 2092.668 kb on - strand, within Pf6N2E2_1771 at 2092.903 kb on + strand, within Pf6N2E2_1771 at 2092.903 kb on + strand, within Pf6N2E2_1771 at 2092.904 kb on - strand, within Pf6N2E2_1771 at 2093.014 kb on - strand, within Pf6N2E2_1771 at 2093.022 kb on + strand, within Pf6N2E2_1771 at 2093.022 kb on + strand, within Pf6N2E2_1771 at 2093.022 kb on + strand, within Pf6N2E2_1771 at 2093.022 kb on + strand, within Pf6N2E2_1771 at 2093.022 kb on + strand, within Pf6N2E2_1771 at 2093.022 kb on + strand, within Pf6N2E2_1771 at 2093.023 kb on - strand, within Pf6N2E2_1771 at 2093.023 kb on - strand, within Pf6N2E2_1771 at 2093.023 kb on - strand, within Pf6N2E2_1771 at 2093.023 kb on - strand, within Pf6N2E2_1771 at 2093.049 kb on - strand, within Pf6N2E2_1771 at 2093.088 kb on - strand, within Pf6N2E2_1771 at 2093.088 kb on - strand, within Pf6N2E2_1771 at 2093.088 kb on - strand, within Pf6N2E2_1771 at 2093.187 kb on + strand, within Pf6N2E2_1771 at 2093.187 kb on + strand, within Pf6N2E2_1771 at 2093.188 kb on - strand, within Pf6N2E2_1771 at 2093.329 kb on - strand, within Pf6N2E2_1771 at 2093.329 kb on - strand, within Pf6N2E2_1771
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 2,090,425 + Pf6N2E2_1768 0.61 +0.3 2,090,425 + Pf6N2E2_1768 0.61 +0.4 2,090,426 - Pf6N2E2_1768 0.61 +0.3 2,090,476 + Pf6N2E2_1768 0.64 +0.6 2,090,696 + Pf6N2E2_1768 0.80 -1.4 2,090,696 + Pf6N2E2_1768 0.80 -3.0 2,090,843 - +1.0 2,090,883 - -0.4 2,090,883 - -0.8 2,091,220 + Pf6N2E2_1769 0.70 +1.6 2,091,220 + Pf6N2E2_1769 0.70 -0.0 2,091,220 + Pf6N2E2_1769 0.70 +0.7 2,091,220 + Pf6N2E2_1769 0.70 +0.3 2,091,220 + Pf6N2E2_1769 0.70 +1.6 2,091,220 + Pf6N2E2_1769 0.70 -1.2 2,091,221 - Pf6N2E2_1769 0.71 +1.6 2,091,221 - Pf6N2E2_1769 0.71 +0.5 2,091,221 - Pf6N2E2_1769 0.71 +0.1 2,091,221 - Pf6N2E2_1769 0.71 -0.4 2,091,224 + Pf6N2E2_1769 0.72 +1.1 2,091,224 + Pf6N2E2_1769 0.72 -0.6 2,091,225 - Pf6N2E2_1769 0.72 +0.6 2,091,225 - Pf6N2E2_1769 0.72 -2.1 2,091,225 - Pf6N2E2_1769 0.72 -0.0 2,091,225 - Pf6N2E2_1769 0.72 +0.2 2,091,280 + Pf6N2E2_1769 0.88 -0.4 2,091,303 + -0.2 2,091,304 - +1.0 2,091,304 - +1.6 2,091,304 - +0.1 2,091,308 - +1.4 2,091,388 - -1.2 2,091,434 + -0.8 2,091,434 + -1.0 2,091,434 + +0.6 2,091,449 + +1.0 2,091,507 - Pf6N2E2_1770 0.13 +1.2 2,091,507 - Pf6N2E2_1770 0.13 +0.5 2,091,729 - Pf6N2E2_1770 0.34 +0.1 2,091,729 - Pf6N2E2_1770 0.34 +0.2 2,091,792 + Pf6N2E2_1770 0.40 +1.6 2,091,792 + Pf6N2E2_1770 0.40 -0.6 2,091,792 + Pf6N2E2_1770 0.40 +1.4 2,091,793 - Pf6N2E2_1770 0.40 +0.2 2,091,793 - Pf6N2E2_1770 0.40 +0.6 2,091,905 + Pf6N2E2_1770 0.50 -2.5 2,091,906 - Pf6N2E2_1770 0.50 +0.2 2,091,906 - Pf6N2E2_1770 0.50 +0.5 2,091,906 - Pf6N2E2_1770 0.50 -0.8 2,091,945 - Pf6N2E2_1770 0.54 +2.2 2,091,983 + Pf6N2E2_1770 0.58 -0.0 2,091,993 - Pf6N2E2_1770 0.58 +1.6 2,091,993 - Pf6N2E2_1770 0.58 -0.8 2,092,010 + Pf6N2E2_1770 0.60 -0.8 2,092,010 + Pf6N2E2_1770 0.60 -1.8 2,092,011 - Pf6N2E2_1770 0.60 -0.4 2,092,122 - Pf6N2E2_1770 0.70 +0.7 2,092,217 + Pf6N2E2_1770 0.79 -0.2 2,092,217 + Pf6N2E2_1770 0.79 -0.7 2,092,217 + Pf6N2E2_1770 0.79 -0.8 2,092,217 + Pf6N2E2_1770 0.79 +1.4 2,092,217 + Pf6N2E2_1770 0.79 +0.6 2,092,218 - Pf6N2E2_1770 0.79 -0.3 2,092,266 - Pf6N2E2_1770 0.84 +1.6 2,092,331 + +0.9 2,092,365 + +0.7 2,092,423 + -0.3 2,092,424 - +0.4 2,092,532 - +2.1 2,092,639 - Pf6N2E2_1771 0.15 +0.6 2,092,641 - Pf6N2E2_1771 0.15 -1.2 2,092,641 - Pf6N2E2_1771 0.15 +0.0 2,092,657 - Pf6N2E2_1771 0.17 +1.6 2,092,668 - Pf6N2E2_1771 0.18 -0.1 2,092,668 - Pf6N2E2_1771 0.18 +1.6 2,092,668 - Pf6N2E2_1771 0.18 +0.2 2,092,668 - Pf6N2E2_1771 0.18 +0.1 2,092,903 + Pf6N2E2_1771 0.42 -0.4 2,092,903 + Pf6N2E2_1771 0.42 +0.7 2,092,904 - Pf6N2E2_1771 0.42 -0.9 2,093,014 - Pf6N2E2_1771 0.53 -1.3 2,093,022 + Pf6N2E2_1771 0.54 +0.6 2,093,022 + Pf6N2E2_1771 0.54 +1.0 2,093,022 + Pf6N2E2_1771 0.54 -0.1 2,093,022 + Pf6N2E2_1771 0.54 -0.7 2,093,022 + Pf6N2E2_1771 0.54 +0.9 2,093,022 + Pf6N2E2_1771 0.54 +0.3 2,093,023 - Pf6N2E2_1771 0.54 -0.5 2,093,023 - Pf6N2E2_1771 0.54 +0.7 2,093,023 - Pf6N2E2_1771 0.54 -0.0 2,093,023 - Pf6N2E2_1771 0.54 -1.4 2,093,049 - Pf6N2E2_1771 0.56 +0.7 2,093,088 - Pf6N2E2_1771 0.60 -0.8 2,093,088 - Pf6N2E2_1771 0.60 -0.8 2,093,088 - Pf6N2E2_1771 0.60 +0.6 2,093,187 + Pf6N2E2_1771 0.71 -0.3 2,093,187 + Pf6N2E2_1771 0.71 +0.4 2,093,188 - Pf6N2E2_1771 0.71 -1.0 2,093,329 - Pf6N2E2_1771 0.85 -0.4 2,093,329 - Pf6N2E2_1771 0.85 +1.6
Or see this region's nucleotide sequence