Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1745

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1744 and Pf6N2E2_1745 are separated by 143 nucleotidesPf6N2E2_1745 and Pf6N2E2_1746 are separated by 12 nucleotidesPf6N2E2_1746 and Pf6N2E2_1747 are separated by 73 nucleotides Pf6N2E2_1744 - ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1), at 2,057,416 to 2,058,468 _1744 Pf6N2E2_1745 - Spermidine Putrescine ABC transporter permease component PotB (TC 3.A.1.11.1), at 2,058,612 to 2,059,793 _1745 Pf6N2E2_1746 - Spermidine Putrescine ABC transporter permease component potC (TC_3.A.1.11.1), at 2,059,806 to 2,060,624 _1746 Pf6N2E2_1747 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 2,060,698 to 2,061,432 _1747 Position (kb) 2058 2059 2060Strain fitness (log2 ratio) -2 -1 0 1at 2057.821 kb on + strand, within Pf6N2E2_1744at 2057.821 kb on + strand, within Pf6N2E2_1744at 2058.116 kb on - strand, within Pf6N2E2_1744at 2058.185 kb on - strand, within Pf6N2E2_1744at 2058.238 kb on + strand, within Pf6N2E2_1744at 2058.253 kb on + strand, within Pf6N2E2_1744at 2058.253 kb on + strand, within Pf6N2E2_1744at 2058.253 kb on + strand, within Pf6N2E2_1744at 2058.466 kb on + strandat 2058.467 kb on - strandat 2058.540 kb on - strandat 2058.640 kb on - strandat 2058.640 kb on - strandat 2058.696 kb on - strandat 2058.737 kb on - strand, within Pf6N2E2_1745at 2058.828 kb on - strand, within Pf6N2E2_1745at 2059.048 kb on - strand, within Pf6N2E2_1745at 2059.119 kb on + strand, within Pf6N2E2_1745at 2059.150 kb on + strand, within Pf6N2E2_1745at 2059.151 kb on - strand, within Pf6N2E2_1745at 2059.151 kb on - strand, within Pf6N2E2_1745at 2059.399 kb on - strand, within Pf6N2E2_1745at 2059.447 kb on - strand, within Pf6N2E2_1745at 2059.447 kb on - strand, within Pf6N2E2_1745at 2059.533 kb on + strand, within Pf6N2E2_1745at 2059.534 kb on - strand, within Pf6N2E2_1745at 2059.600 kb on - strand, within Pf6N2E2_1745at 2059.603 kb on - strand, within Pf6N2E2_1745at 2059.665 kb on + strand, within Pf6N2E2_1745at 2059.666 kb on - strand, within Pf6N2E2_1745at 2059.740 kb on + strandat 2059.740 kb on + strandat 2059.740 kb on + strandat 2059.791 kb on + strandat 2059.873 kb on + strandat 2059.873 kb on + strandat 2059.907 kb on + strand, within Pf6N2E2_1746at 2059.907 kb on + strand, within Pf6N2E2_1746at 2059.969 kb on - strand, within Pf6N2E2_1746at 2060.116 kb on + strand, within Pf6N2E2_1746at 2060.117 kb on - strand, within Pf6N2E2_1746at 2060.175 kb on + strand, within Pf6N2E2_1746at 2060.175 kb on + strand, within Pf6N2E2_1746at 2060.175 kb on + strand, within Pf6N2E2_1746at 2060.176 kb on - strand, within Pf6N2E2_1746at 2060.701 kb on + strandat 2060.701 kb on + strandat 2060.701 kb on + strandat 2060.702 kb on - strandat 2060.778 kb on - strand, within Pf6N2E2_1747

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
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2,057,821 + Pf6N2E2_1744 0.38 +0.2
2,057,821 + Pf6N2E2_1744 0.38 +0.7
2,058,116 - Pf6N2E2_1744 0.66 -0.9
2,058,185 - Pf6N2E2_1744 0.73 +1.6
2,058,238 + Pf6N2E2_1744 0.78 +1.6
2,058,253 + Pf6N2E2_1744 0.79 -0.2
2,058,253 + Pf6N2E2_1744 0.79 -0.0
2,058,253 + Pf6N2E2_1744 0.79 -0.2
2,058,466 + -0.4
2,058,467 - +0.6
2,058,540 - -0.7
2,058,640 - +0.7
2,058,640 - -0.3
2,058,696 - -0.0
2,058,737 - Pf6N2E2_1745 0.11 -0.3
2,058,828 - Pf6N2E2_1745 0.18 +1.6
2,059,048 - Pf6N2E2_1745 0.37 -1.2
2,059,119 + Pf6N2E2_1745 0.43 +1.6
2,059,150 + Pf6N2E2_1745 0.46 -0.3
2,059,151 - Pf6N2E2_1745 0.46 -0.4
2,059,151 - Pf6N2E2_1745 0.46 -0.3
2,059,399 - Pf6N2E2_1745 0.67 -0.4
2,059,447 - Pf6N2E2_1745 0.71 +0.7
2,059,447 - Pf6N2E2_1745 0.71 -0.1
2,059,533 + Pf6N2E2_1745 0.78 +1.2
2,059,534 - Pf6N2E2_1745 0.78 +1.0
2,059,600 - Pf6N2E2_1745 0.84 +1.6
2,059,603 - Pf6N2E2_1745 0.84 -1.2
2,059,665 + Pf6N2E2_1745 0.89 +0.2
2,059,666 - Pf6N2E2_1745 0.89 -0.4
2,059,740 + -1.1
2,059,740 + -1.3
2,059,740 + +0.2
2,059,791 + +0.6
2,059,873 + +1.0
2,059,873 + -2.2
2,059,907 + Pf6N2E2_1746 0.12 -0.6
2,059,907 + Pf6N2E2_1746 0.12 +0.4
2,059,969 - Pf6N2E2_1746 0.20 -0.6
2,060,116 + Pf6N2E2_1746 0.38 -1.4
2,060,117 - Pf6N2E2_1746 0.38 +0.8
2,060,175 + Pf6N2E2_1746 0.45 -0.2
2,060,175 + Pf6N2E2_1746 0.45 +0.8
2,060,175 + Pf6N2E2_1746 0.45 -0.9
2,060,176 - Pf6N2E2_1746 0.45 -1.0
2,060,701 + +0.6
2,060,701 + +1.3
2,060,701 + +1.6
2,060,702 - +0.5
2,060,778 - Pf6N2E2_1747 0.11 +0.1

Or see this region's nucleotide sequence