Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1743 and Pf6N2E2_1744 are separated by 151 nucleotides Pf6N2E2_1744 and Pf6N2E2_1745 are separated by 143 nucleotides
Pf6N2E2_1743 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 2,056,158 to 2,057,264
_1743
Pf6N2E2_1744 - ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1), at 2,057,416 to 2,058,468
_1744
Pf6N2E2_1745 - Spermidine Putrescine ABC transporter permease component PotB (TC 3.A.1.11.1), at 2,058,612 to 2,059,793
_1745
Position (kb)
2057
2058
2059 Strain fitness (log2 ratio)
-1
0
1 at 2056.444 kb on - strand, within Pf6N2E2_1743 at 2056.714 kb on - strand, within Pf6N2E2_1743 at 2056.758 kb on + strand, within Pf6N2E2_1743 at 2057.248 kb on + strand at 2057.248 kb on + strand at 2057.249 kb on - strand at 2057.249 kb on - strand at 2057.288 kb on + strand at 2057.289 kb on - strand at 2057.289 kb on - strand at 2057.348 kb on + strand at 2057.348 kb on + strand at 2057.387 kb on + strand at 2057.388 kb on - strand at 2057.388 kb on - strand at 2057.388 kb on - strand at 2057.471 kb on + strand at 2057.471 kb on + strand at 2057.472 kb on - strand at 2057.605 kb on + strand, within Pf6N2E2_1744 at 2057.605 kb on + strand, within Pf6N2E2_1744 at 2057.606 kb on - strand, within Pf6N2E2_1744 at 2057.821 kb on + strand, within Pf6N2E2_1744 at 2057.821 kb on + strand, within Pf6N2E2_1744 at 2058.116 kb on - strand, within Pf6N2E2_1744 at 2058.185 kb on - strand, within Pf6N2E2_1744 at 2058.238 kb on + strand, within Pf6N2E2_1744 at 2058.253 kb on + strand, within Pf6N2E2_1744 at 2058.253 kb on + strand, within Pf6N2E2_1744 at 2058.253 kb on + strand, within Pf6N2E2_1744 at 2058.466 kb on + strand at 2058.467 kb on - strand at 2058.540 kb on - strand at 2058.640 kb on - strand at 2058.640 kb on - strand at 2058.696 kb on - strand at 2058.737 kb on - strand, within Pf6N2E2_1745 at 2058.828 kb on - strand, within Pf6N2E2_1745 at 2059.048 kb on - strand, within Pf6N2E2_1745 at 2059.119 kb on + strand, within Pf6N2E2_1745 at 2059.150 kb on + strand, within Pf6N2E2_1745 at 2059.151 kb on - strand, within Pf6N2E2_1745 at 2059.151 kb on - strand, within Pf6N2E2_1745 at 2059.399 kb on - strand, within Pf6N2E2_1745 at 2059.447 kb on - strand, within Pf6N2E2_1745 at 2059.447 kb on - strand, within Pf6N2E2_1745
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 2,056,444 - Pf6N2E2_1743 0.26 -0.4 2,056,714 - Pf6N2E2_1743 0.50 -0.4 2,056,758 + Pf6N2E2_1743 0.54 -0.7 2,057,248 + +1.3 2,057,248 + -0.7 2,057,249 - +0.5 2,057,249 - -0.0 2,057,288 + +1.6 2,057,289 - +0.1 2,057,289 - -0.4 2,057,348 + -0.8 2,057,348 + +1.2 2,057,387 + +0.2 2,057,388 - +0.1 2,057,388 - +0.6 2,057,388 - -1.2 2,057,471 + -0.0 2,057,471 + +0.8 2,057,472 - +0.6 2,057,605 + Pf6N2E2_1744 0.18 -0.8 2,057,605 + Pf6N2E2_1744 0.18 +1.0 2,057,606 - Pf6N2E2_1744 0.18 +0.4 2,057,821 + Pf6N2E2_1744 0.38 +0.2 2,057,821 + Pf6N2E2_1744 0.38 +0.7 2,058,116 - Pf6N2E2_1744 0.66 -0.9 2,058,185 - Pf6N2E2_1744 0.73 +1.6 2,058,238 + Pf6N2E2_1744 0.78 +1.6 2,058,253 + Pf6N2E2_1744 0.79 -0.2 2,058,253 + Pf6N2E2_1744 0.79 -0.0 2,058,253 + Pf6N2E2_1744 0.79 -0.2 2,058,466 + -0.4 2,058,467 - +0.6 2,058,540 - -0.7 2,058,640 - +0.7 2,058,640 - -0.3 2,058,696 - -0.0 2,058,737 - Pf6N2E2_1745 0.11 -0.3 2,058,828 - Pf6N2E2_1745 0.18 +1.6 2,059,048 - Pf6N2E2_1745 0.37 -1.2 2,059,119 + Pf6N2E2_1745 0.43 +1.6 2,059,150 + Pf6N2E2_1745 0.46 -0.3 2,059,151 - Pf6N2E2_1745 0.46 -0.4 2,059,151 - Pf6N2E2_1745 0.46 -0.3 2,059,399 - Pf6N2E2_1745 0.67 -0.4 2,059,447 - Pf6N2E2_1745 0.71 +0.7 2,059,447 - Pf6N2E2_1745 0.71 -0.1
Or see this region's nucleotide sequence