Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1744

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1743 and Pf6N2E2_1744 are separated by 151 nucleotidesPf6N2E2_1744 and Pf6N2E2_1745 are separated by 143 nucleotides Pf6N2E2_1743 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 2,056,158 to 2,057,264 _1743 Pf6N2E2_1744 - ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1), at 2,057,416 to 2,058,468 _1744 Pf6N2E2_1745 - Spermidine Putrescine ABC transporter permease component PotB (TC 3.A.1.11.1), at 2,058,612 to 2,059,793 _1745 Position (kb) 2057 2058 2059Strain fitness (log2 ratio) -1 0 1at 2056.444 kb on - strand, within Pf6N2E2_1743at 2056.714 kb on - strand, within Pf6N2E2_1743at 2056.758 kb on + strand, within Pf6N2E2_1743at 2057.248 kb on + strandat 2057.248 kb on + strandat 2057.249 kb on - strandat 2057.249 kb on - strandat 2057.288 kb on + strandat 2057.289 kb on - strandat 2057.289 kb on - strandat 2057.348 kb on + strandat 2057.348 kb on + strandat 2057.387 kb on + strandat 2057.388 kb on - strandat 2057.388 kb on - strandat 2057.388 kb on - strandat 2057.471 kb on + strandat 2057.471 kb on + strandat 2057.472 kb on - strandat 2057.605 kb on + strand, within Pf6N2E2_1744at 2057.605 kb on + strand, within Pf6N2E2_1744at 2057.606 kb on - strand, within Pf6N2E2_1744at 2057.821 kb on + strand, within Pf6N2E2_1744at 2057.821 kb on + strand, within Pf6N2E2_1744at 2058.116 kb on - strand, within Pf6N2E2_1744at 2058.185 kb on - strand, within Pf6N2E2_1744at 2058.238 kb on + strand, within Pf6N2E2_1744at 2058.253 kb on + strand, within Pf6N2E2_1744at 2058.253 kb on + strand, within Pf6N2E2_1744at 2058.253 kb on + strand, within Pf6N2E2_1744at 2058.466 kb on + strandat 2058.467 kb on - strandat 2058.540 kb on - strandat 2058.640 kb on - strandat 2058.640 kb on - strandat 2058.696 kb on - strandat 2058.737 kb on - strand, within Pf6N2E2_1745at 2058.828 kb on - strand, within Pf6N2E2_1745at 2059.048 kb on - strand, within Pf6N2E2_1745at 2059.119 kb on + strand, within Pf6N2E2_1745at 2059.150 kb on + strand, within Pf6N2E2_1745at 2059.151 kb on - strand, within Pf6N2E2_1745at 2059.151 kb on - strand, within Pf6N2E2_1745at 2059.399 kb on - strand, within Pf6N2E2_1745at 2059.447 kb on - strand, within Pf6N2E2_1745at 2059.447 kb on - strand, within Pf6N2E2_1745

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
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2,056,444 - Pf6N2E2_1743 0.26 -0.4
2,056,714 - Pf6N2E2_1743 0.50 -0.4
2,056,758 + Pf6N2E2_1743 0.54 -0.7
2,057,248 + +1.3
2,057,248 + -0.7
2,057,249 - +0.5
2,057,249 - -0.0
2,057,288 + +1.6
2,057,289 - +0.1
2,057,289 - -0.4
2,057,348 + -0.8
2,057,348 + +1.2
2,057,387 + +0.2
2,057,388 - +0.1
2,057,388 - +0.6
2,057,388 - -1.2
2,057,471 + -0.0
2,057,471 + +0.8
2,057,472 - +0.6
2,057,605 + Pf6N2E2_1744 0.18 -0.8
2,057,605 + Pf6N2E2_1744 0.18 +1.0
2,057,606 - Pf6N2E2_1744 0.18 +0.4
2,057,821 + Pf6N2E2_1744 0.38 +0.2
2,057,821 + Pf6N2E2_1744 0.38 +0.7
2,058,116 - Pf6N2E2_1744 0.66 -0.9
2,058,185 - Pf6N2E2_1744 0.73 +1.6
2,058,238 + Pf6N2E2_1744 0.78 +1.6
2,058,253 + Pf6N2E2_1744 0.79 -0.2
2,058,253 + Pf6N2E2_1744 0.79 -0.0
2,058,253 + Pf6N2E2_1744 0.79 -0.2
2,058,466 + -0.4
2,058,467 - +0.6
2,058,540 - -0.7
2,058,640 - +0.7
2,058,640 - -0.3
2,058,696 - -0.0
2,058,737 - Pf6N2E2_1745 0.11 -0.3
2,058,828 - Pf6N2E2_1745 0.18 +1.6
2,059,048 - Pf6N2E2_1745 0.37 -1.2
2,059,119 + Pf6N2E2_1745 0.43 +1.6
2,059,150 + Pf6N2E2_1745 0.46 -0.3
2,059,151 - Pf6N2E2_1745 0.46 -0.4
2,059,151 - Pf6N2E2_1745 0.46 -0.3
2,059,399 - Pf6N2E2_1745 0.67 -0.4
2,059,447 - Pf6N2E2_1745 0.71 +0.7
2,059,447 - Pf6N2E2_1745 0.71 -0.1

Or see this region's nucleotide sequence