Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1672 and Pf6N2E2_1673 are separated by 19 nucleotides Pf6N2E2_1673 and Pf6N2E2_1674 are separated by 608 nucleotides
Pf6N2E2_1672 - hypothetical protein, at 1,978,161 to 1,979,042
_1672
Pf6N2E2_1673 - Transcriptional regulator, TetR family, at 1,979,062 to 1,979,499
_1673
Pf6N2E2_1674 - Hydroxymethylglutaryl-CoA synthase (EC 2.3.3.10), at 1,980,108 to 1,981,190
_1674
Position (kb)
1979
1980 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1978.063 kb on + strand at 1978.063 kb on + strand at 1978.067 kb on + strand at 1978.067 kb on + strand at 1978.068 kb on - strand at 1978.068 kb on - strand at 1978.092 kb on + strand at 1978.093 kb on - strand at 1978.143 kb on + strand at 1978.143 kb on + strand at 1978.144 kb on - strand at 1978.144 kb on - strand at 1978.144 kb on - strand at 1978.154 kb on + strand at 1978.155 kb on - strand at 1978.155 kb on - strand at 1978.174 kb on + strand at 1978.191 kb on + strand at 1978.254 kb on + strand, within Pf6N2E2_1672 at 1978.408 kb on - strand, within Pf6N2E2_1672 at 1978.435 kb on - strand, within Pf6N2E2_1672 at 1978.435 kb on - strand, within Pf6N2E2_1672 at 1978.435 kb on - strand, within Pf6N2E2_1672 at 1978.435 kb on - strand, within Pf6N2E2_1672 at 1978.521 kb on + strand, within Pf6N2E2_1672 at 1978.540 kb on - strand, within Pf6N2E2_1672 at 1978.614 kb on + strand, within Pf6N2E2_1672 at 1978.615 kb on - strand, within Pf6N2E2_1672 at 1978.615 kb on - strand, within Pf6N2E2_1672 at 1978.615 kb on - strand, within Pf6N2E2_1672 at 1978.615 kb on - strand, within Pf6N2E2_1672 at 1978.622 kb on + strand, within Pf6N2E2_1672 at 1978.623 kb on - strand, within Pf6N2E2_1672 at 1978.623 kb on - strand, within Pf6N2E2_1672 at 1978.623 kb on - strand, within Pf6N2E2_1672 at 1978.623 kb on - strand, within Pf6N2E2_1672 at 1978.623 kb on - strand, within Pf6N2E2_1672 at 1978.623 kb on - strand, within Pf6N2E2_1672 at 1978.642 kb on - strand, within Pf6N2E2_1672 at 1978.642 kb on - strand, within Pf6N2E2_1672 at 1978.732 kb on + strand, within Pf6N2E2_1672 at 1978.733 kb on - strand, within Pf6N2E2_1672 at 1978.780 kb on + strand, within Pf6N2E2_1672 at 1978.780 kb on + strand, within Pf6N2E2_1672 at 1978.780 kb on + strand, within Pf6N2E2_1672 at 1978.830 kb on + strand, within Pf6N2E2_1672 at 1978.831 kb on - strand, within Pf6N2E2_1672 at 1978.906 kb on + strand, within Pf6N2E2_1672 at 1978.907 kb on - strand, within Pf6N2E2_1672 at 1979.040 kb on + strand at 1979.040 kb on + strand at 1979.227 kb on - strand, within Pf6N2E2_1673 at 1979.227 kb on - strand, within Pf6N2E2_1673 at 1979.446 kb on - strand, within Pf6N2E2_1673 at 1979.483 kb on + strand at 1979.484 kb on - strand at 1979.525 kb on + strand at 1979.526 kb on - strand at 1979.598 kb on + strand at 1979.599 kb on - strand at 1979.599 kb on - strand at 1979.599 kb on - strand at 1979.599 kb on - strand at 1979.608 kb on + strand at 1979.609 kb on - strand at 1979.688 kb on + strand at 1979.689 kb on - strand at 1979.778 kb on + strand at 1979.781 kb on - strand at 1979.781 kb on - strand at 1979.785 kb on - strand at 1979.785 kb on - strand at 1979.785 kb on - strand at 1979.787 kb on - strand at 1979.787 kb on - strand at 1979.828 kb on + strand at 1979.829 kb on - strand at 1979.829 kb on - strand at 1979.829 kb on - strand at 1979.842 kb on - strand at 1979.849 kb on + strand at 1979.849 kb on + strand at 1979.850 kb on - strand at 1979.910 kb on + strand at 1979.910 kb on + strand at 1979.914 kb on + strand at 1979.937 kb on + strand at 1979.986 kb on + strand at 1980.008 kb on + strand at 1980.043 kb on + strand at 1980.103 kb on + strand at 1980.103 kb on + strand at 1980.103 kb on + strand at 1980.103 kb on + strand at 1980.103 kb on + strand at 1980.103 kb on + strand at 1980.104 kb on - strand at 1980.113 kb on + strand at 1980.113 kb on + strand at 1980.113 kb on + strand at 1980.113 kb on + strand at 1980.113 kb on + strand at 1980.113 kb on + strand at 1980.114 kb on - strand at 1980.118 kb on + strand at 1980.132 kb on + strand at 1980.211 kb on + strand at 1980.211 kb on + strand at 1980.269 kb on + strand, within Pf6N2E2_1674 at 1980.269 kb on + strand, within Pf6N2E2_1674 at 1980.269 kb on + strand, within Pf6N2E2_1674 at 1980.360 kb on + strand, within Pf6N2E2_1674 at 1980.360 kb on + strand, within Pf6N2E2_1674 at 1980.360 kb on + strand, within Pf6N2E2_1674 at 1980.361 kb on - strand, within Pf6N2E2_1674 at 1980.477 kb on + strand, within Pf6N2E2_1674 at 1980.487 kb on + strand, within Pf6N2E2_1674
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 1,978,063 + +0.6 1,978,063 + -0.2 1,978,067 + +0.8 1,978,067 + +0.4 1,978,068 - +0.6 1,978,068 - +0.0 1,978,092 + +0.1 1,978,093 - -0.4 1,978,143 + +0.1 1,978,143 + +0.2 1,978,144 - -1.2 1,978,144 - -0.9 1,978,144 - -0.9 1,978,154 + +0.1 1,978,155 - +2.6 1,978,155 - +1.6 1,978,174 + +1.1 1,978,191 + +0.6 1,978,254 + Pf6N2E2_1672 0.11 -0.1 1,978,408 - Pf6N2E2_1672 0.28 +0.4 1,978,435 - Pf6N2E2_1672 0.31 -0.7 1,978,435 - Pf6N2E2_1672 0.31 +0.1 1,978,435 - Pf6N2E2_1672 0.31 -1.4 1,978,435 - Pf6N2E2_1672 0.31 -0.3 1,978,521 + Pf6N2E2_1672 0.41 +0.6 1,978,540 - Pf6N2E2_1672 0.43 +1.1 1,978,614 + Pf6N2E2_1672 0.51 +0.3 1,978,615 - Pf6N2E2_1672 0.51 -0.0 1,978,615 - Pf6N2E2_1672 0.51 -0.0 1,978,615 - Pf6N2E2_1672 0.51 -0.3 1,978,615 - Pf6N2E2_1672 0.51 -0.7 1,978,622 + Pf6N2E2_1672 0.52 +0.1 1,978,623 - Pf6N2E2_1672 0.52 +1.0 1,978,623 - Pf6N2E2_1672 0.52 -0.6 1,978,623 - Pf6N2E2_1672 0.52 -0.3 1,978,623 - Pf6N2E2_1672 0.52 +0.6 1,978,623 - Pf6N2E2_1672 0.52 -0.6 1,978,623 - Pf6N2E2_1672 0.52 -0.0 1,978,642 - Pf6N2E2_1672 0.55 +0.6 1,978,642 - Pf6N2E2_1672 0.55 -0.6 1,978,732 + Pf6N2E2_1672 0.65 -0.8 1,978,733 - Pf6N2E2_1672 0.65 -0.8 1,978,780 + Pf6N2E2_1672 0.70 +0.2 1,978,780 + Pf6N2E2_1672 0.70 -0.0 1,978,780 + Pf6N2E2_1672 0.70 -0.1 1,978,830 + Pf6N2E2_1672 0.76 +0.2 1,978,831 - Pf6N2E2_1672 0.76 -1.0 1,978,906 + Pf6N2E2_1672 0.84 -1.2 1,978,907 - Pf6N2E2_1672 0.85 +2.6 1,979,040 + -0.4 1,979,040 + -0.2 1,979,227 - Pf6N2E2_1673 0.38 -2.2 1,979,227 - Pf6N2E2_1673 0.38 +1.6 1,979,446 - Pf6N2E2_1673 0.88 -0.6 1,979,483 + -0.0 1,979,484 - -1.8 1,979,525 + -0.4 1,979,526 - -0.3 1,979,598 + -2.3 1,979,599 - -0.2 1,979,599 - +1.6 1,979,599 - -0.4 1,979,599 - -0.2 1,979,608 + -2.1 1,979,609 - -1.3 1,979,688 + -0.7 1,979,689 - -0.4 1,979,778 + +0.6 1,979,781 - +1.3 1,979,781 - -0.3 1,979,785 - -0.3 1,979,785 - -0.1 1,979,785 - -0.5 1,979,787 - +0.5 1,979,787 - +0.6 1,979,828 + -0.9 1,979,829 - +0.4 1,979,829 - -1.2 1,979,829 - +1.6 1,979,842 - -0.1 1,979,849 + -1.3 1,979,849 + +0.0 1,979,850 - +0.6 1,979,910 + -0.4 1,979,910 + -0.8 1,979,914 + -0.4 1,979,937 + +1.6 1,979,986 + +0.6 1,980,008 + -2.2 1,980,043 + -0.4 1,980,103 + -0.4 1,980,103 + -0.1 1,980,103 + -0.2 1,980,103 + -1.0 1,980,103 + -2.1 1,980,103 + -0.2 1,980,104 - +1.4 1,980,113 + -0.1 1,980,113 + -0.7 1,980,113 + +0.0 1,980,113 + +1.6 1,980,113 + +1.7 1,980,113 + +0.3 1,980,114 - -0.7 1,980,118 + -1.0 1,980,132 + -1.3 1,980,211 + +0.0 1,980,211 + +0.1 1,980,269 + Pf6N2E2_1674 0.15 +1.6 1,980,269 + Pf6N2E2_1674 0.15 +0.4 1,980,269 + Pf6N2E2_1674 0.15 +0.3 1,980,360 + Pf6N2E2_1674 0.23 -0.7 1,980,360 + Pf6N2E2_1674 0.23 +1.6 1,980,360 + Pf6N2E2_1674 0.23 -1.3 1,980,361 - Pf6N2E2_1674 0.23 +1.6 1,980,477 + Pf6N2E2_1674 0.34 +0.3 1,980,487 + Pf6N2E2_1674 0.35 -0.9
Or see this region's nucleotide sequence