Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1664

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
1,969,810	+				+0.3
1,969,810	+				+1.6
1,969,855	+				-2.2
1,969,921	-				+0.5
1,969,921	-				-1.9
1,969,993	-		Pf6N2E2_1663	0.19	-0.0
1,970,274	+		Pf6N2E2_1663	0.57	+1.3
1,970,274	+		Pf6N2E2_1663	0.57	-0.2
1,970,333	+		Pf6N2E2_1663	0.65	-0.5
1,970,333	+		Pf6N2E2_1663	0.65	+0.2
1,970,333	+		Pf6N2E2_1663	0.65	+0.0
1,970,334	-		Pf6N2E2_1663	0.65	-0.3
1,970,334	-		Pf6N2E2_1663	0.65	+0.9
1,970,334	-		Pf6N2E2_1663	0.65	-1.3
1,970,353	-		Pf6N2E2_1663	0.68	+0.2
1,970,353	-		Pf6N2E2_1663	0.68	+0.2
1,970,567	+				+0.1
1,970,582	+				+0.6
1,970,583	-				-0.1
1,970,699	-				+0.6
1,970,699	-				-0.1
1,970,699	-				-0.7
1,970,699	-				+0.0
1,970,702	+				+0.5
1,970,702	+				-0.4
1,970,702	+				+0.2
1,970,702	+				+1.6
1,970,702	+				+0.9
1,970,702	+				-0.2
1,970,702	+				-1.2
1,970,702	+				-0.0
1,970,702	+				-0.1
1,970,703	-				+1.2
1,970,703	-				-0.2
1,970,703	-				+3.0
1,971,137	+		Pf6N2E2_1664	0.40	+0.6
1,971,427	+		Pf6N2E2_1664	0.61	+1.6
1,971,509	+		Pf6N2E2_1664	0.67	-0.4
1,971,510	-		Pf6N2E2_1664	0.67	-1.1
1,971,518	+		Pf6N2E2_1664	0.67	+1.6
1,971,519	-		Pf6N2E2_1664	0.67	-0.7
1,971,519	-		Pf6N2E2_1664	0.67	-0.3
1,971,578	+		Pf6N2E2_1664	0.72	+2.2
1,971,578	+		Pf6N2E2_1664	0.72	-0.0
1,971,578	+		Pf6N2E2_1664	0.72	+0.3
1,971,578	+		Pf6N2E2_1664	0.72	-1.6
1,971,579	-		Pf6N2E2_1664	0.72	+0.7
1,971,579	-		Pf6N2E2_1664	0.72	-0.6
1,971,579	-		Pf6N2E2_1664	0.72	-0.4
1,971,630	-		Pf6N2E2_1664	0.75	+2.0
1,971,770	+		Pf6N2E2_1664	0.86	-0.1
1,971,770	+		Pf6N2E2_1664	0.86	-0.3
1,971,770	+		Pf6N2E2_1664	0.86	+0.9
1,971,770	+		Pf6N2E2_1664	0.86	-1.3
1,971,771	-		Pf6N2E2_1664	0.86	+0.8
1,971,854	+				+1.4
1,971,854	+				-0.2
1,971,855	-				+0.6
1,971,899	+				+0.5
1,971,900	-				+0.2
1,971,974	+				-0.7
1,971,975	-				-0.3
1,971,975	-				-0.8
1,971,975	-				-0.2
1,972,041	+		Pf6N2E2_1665	0.51	+1.7
1,972,041	+		Pf6N2E2_1665	0.51	+0.2
1,972,041	+		Pf6N2E2_1665	0.51	+1.2
1,972,041	+		Pf6N2E2_1665	0.51	-1.3
1,972,042	-		Pf6N2E2_1665	0.52	-0.0
1,972,131	+				-0.6
1,972,131	+				+1.0
1,972,131	+				-1.7
1,972,131	+				-0.6
1,972,206	+				+1.6
1,972,239	+				-0.8
1,972,709	+		Pf6N2E2_1666	0.41	+0.2
1,972,710	-		Pf6N2E2_1666	0.41	-1.0
1,972,710	-		Pf6N2E2_1666	0.41	+1.0
1,972,839	+		Pf6N2E2_1666	0.55	+1.6