Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1598

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1597 and Pf6N2E2_1598 are separated by 11 nucleotidesPf6N2E2_1598 and Pf6N2E2_1599 are separated by 9 nucleotidesPf6N2E2_1599 and Pf6N2E2_1600 are separated by 130 nucleotides Pf6N2E2_1597 - Periplasmic nitrate reductase precursor (EC 1.7.99.4), at 1,868,482 to 1,870,986 _1597 Pf6N2E2_1598 - Nitrate reductase cytochrome c550-type subunit, at 1,870,998 to 1,871,483 _1598 Pf6N2E2_1599 - Cytochrome c-type protein NapC, at 1,871,493 to 1,872,089 _1599 Pf6N2E2_1600 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 1,872,220 to 1,873,872 _1600 Position (kb) 1870 1871 1872Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1870.021 kb on + strand, within Pf6N2E2_1597at 1870.254 kb on + strand, within Pf6N2E2_1597at 1870.328 kb on - strand, within Pf6N2E2_1597at 1870.387 kb on + strand, within Pf6N2E2_1597at 1870.388 kb on - strand, within Pf6N2E2_1597at 1870.520 kb on - strand, within Pf6N2E2_1597at 1870.549 kb on + strand, within Pf6N2E2_1597at 1870.624 kb on + strand, within Pf6N2E2_1597at 1870.729 kb on + strand, within Pf6N2E2_1597at 1870.865 kb on + strandat 1870.866 kb on - strandat 1871.184 kb on + strand, within Pf6N2E2_1598at 1871.319 kb on + strand, within Pf6N2E2_1598at 1871.320 kb on - strand, within Pf6N2E2_1598at 1871.368 kb on - strand, within Pf6N2E2_1598at 1871.404 kb on + strand, within Pf6N2E2_1598at 1871.404 kb on + strand, within Pf6N2E2_1598at 1871.405 kb on - strand, within Pf6N2E2_1598at 1871.405 kb on - strand, within Pf6N2E2_1598at 1871.524 kb on - strandat 1871.553 kb on + strand, within Pf6N2E2_1599at 1871.554 kb on - strand, within Pf6N2E2_1599at 1871.554 kb on - strand, within Pf6N2E2_1599at 1871.554 kb on - strand, within Pf6N2E2_1599at 1871.554 kb on - strand, within Pf6N2E2_1599at 1871.554 kb on - strand, within Pf6N2E2_1599at 1871.554 kb on - strand, within Pf6N2E2_1599at 1871.554 kb on - strand, within Pf6N2E2_1599at 1871.557 kb on - strand, within Pf6N2E2_1599at 1871.557 kb on - strand, within Pf6N2E2_1599at 1871.715 kb on + strand, within Pf6N2E2_1599at 1871.781 kb on - strand, within Pf6N2E2_1599at 1872.224 kb on - strandat 1872.224 kb on - strandat 1872.224 kb on - strandat 1872.224 kb on - strandat 1872.370 kb on + strandat 1872.370 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
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1,870,021 + Pf6N2E2_1597 0.61 -1.1
1,870,254 + Pf6N2E2_1597 0.71 -0.0
1,870,328 - Pf6N2E2_1597 0.74 -1.2
1,870,387 + Pf6N2E2_1597 0.76 +0.5
1,870,388 - Pf6N2E2_1597 0.76 +0.3
1,870,520 - Pf6N2E2_1597 0.81 +0.6
1,870,549 + Pf6N2E2_1597 0.83 +0.1
1,870,624 + Pf6N2E2_1597 0.86 -0.6
1,870,729 + Pf6N2E2_1597 0.90 +1.3
1,870,865 + -0.3
1,870,866 - -0.3
1,871,184 + Pf6N2E2_1598 0.38 -0.4
1,871,319 + Pf6N2E2_1598 0.66 -0.1
1,871,320 - Pf6N2E2_1598 0.66 +0.2
1,871,368 - Pf6N2E2_1598 0.76 +1.6
1,871,404 + Pf6N2E2_1598 0.84 +0.2
1,871,404 + Pf6N2E2_1598 0.84 +2.9
1,871,405 - Pf6N2E2_1598 0.84 +0.2
1,871,405 - Pf6N2E2_1598 0.84 +0.3
1,871,524 - -0.4
1,871,553 + Pf6N2E2_1599 0.10 +1.6
1,871,554 - Pf6N2E2_1599 0.10 +1.6
1,871,554 - Pf6N2E2_1599 0.10 -0.8
1,871,554 - Pf6N2E2_1599 0.10 +1.6
1,871,554 - Pf6N2E2_1599 0.10 -0.1
1,871,554 - Pf6N2E2_1599 0.10 -0.3
1,871,554 - Pf6N2E2_1599 0.10 +0.4
1,871,554 - Pf6N2E2_1599 0.10 -1.0
1,871,557 - Pf6N2E2_1599 0.11 +0.0
1,871,557 - Pf6N2E2_1599 0.11 -0.6
1,871,715 + Pf6N2E2_1599 0.37 -0.2
1,871,781 - Pf6N2E2_1599 0.48 -0.9
1,872,224 - +0.8
1,872,224 - +1.6
1,872,224 - -0.1
1,872,224 - -2.2
1,872,370 + +0.2
1,872,370 + -0.2

Or see this region's nucleotide sequence