Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1596

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1594 and Pf6N2E2_1595 are separated by 196 nucleotidesPf6N2E2_1595 and Pf6N2E2_1596 are separated by 37 nucleotidesPf6N2E2_1596 and Pf6N2E2_1597 overlap by 4 nucleotides Pf6N2E2_1594 - Tia invasion determinant-related protein precursor, at 1,867,125 to 1,867,823 _1594 Pf6N2E2_1595 - Periplasmic nitrate reductase component NapE, at 1,868,020 to 1,868,187 _1595 Pf6N2E2_1596 - Periplasmic nitrate reductase component NapD, at 1,868,225 to 1,868,485 _1596 Pf6N2E2_1597 - Periplasmic nitrate reductase precursor (EC 1.7.99.4), at 1,868,482 to 1,870,986 _1597 Position (kb) 1868 1869Strain fitness (log2 ratio) -2 -1 0 1at 1867.303 kb on - strand, within Pf6N2E2_1594at 1867.431 kb on + strand, within Pf6N2E2_1594at 1867.500 kb on + strand, within Pf6N2E2_1594at 1867.501 kb on - strand, within Pf6N2E2_1594at 1867.545 kb on + strand, within Pf6N2E2_1594at 1867.545 kb on + strand, within Pf6N2E2_1594at 1867.545 kb on + strand, within Pf6N2E2_1594at 1867.545 kb on + strand, within Pf6N2E2_1594at 1867.545 kb on + strand, within Pf6N2E2_1594at 1867.545 kb on + strand, within Pf6N2E2_1594at 1867.546 kb on - strand, within Pf6N2E2_1594at 1867.546 kb on - strand, within Pf6N2E2_1594at 1867.546 kb on - strand, within Pf6N2E2_1594at 1867.711 kb on - strand, within Pf6N2E2_1594at 1867.779 kb on + strandat 1867.812 kb on + strandat 1867.821 kb on + strandat 1867.821 kb on + strandat 1867.821 kb on + strandat 1867.821 kb on + strandat 1867.821 kb on + strandat 1867.952 kb on + strandat 1867.953 kb on - strandat 1867.991 kb on - strandat 1868.162 kb on - strand, within Pf6N2E2_1595at 1868.162 kb on - strand, within Pf6N2E2_1595at 1868.211 kb on - strandat 1868.243 kb on - strandat 1868.243 kb on - strandat 1868.243 kb on - strandat 1868.259 kb on + strand, within Pf6N2E2_1596at 1868.260 kb on - strand, within Pf6N2E2_1596at 1868.761 kb on + strand, within Pf6N2E2_1597at 1868.761 kb on + strand, within Pf6N2E2_1597at 1868.761 kb on + strand, within Pf6N2E2_1597at 1868.761 kb on + strand, within Pf6N2E2_1597at 1868.762 kb on - strand, within Pf6N2E2_1597at 1868.762 kb on - strand, within Pf6N2E2_1597at 1869.116 kb on + strand, within Pf6N2E2_1597at 1869.117 kb on - strand, within Pf6N2E2_1597at 1869.204 kb on - strand, within Pf6N2E2_1597at 1869.296 kb on - strand, within Pf6N2E2_1597at 1869.296 kb on - strand, within Pf6N2E2_1597at 1869.296 kb on - strand, within Pf6N2E2_1597at 1869.296 kb on - strand, within Pf6N2E2_1597

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
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1,867,303 - Pf6N2E2_1594 0.25 +0.4
1,867,431 + Pf6N2E2_1594 0.44 -0.5
1,867,500 + Pf6N2E2_1594 0.54 +0.6
1,867,501 - Pf6N2E2_1594 0.54 -0.3
1,867,545 + Pf6N2E2_1594 0.60 -1.8
1,867,545 + Pf6N2E2_1594 0.60 +0.3
1,867,545 + Pf6N2E2_1594 0.60 -2.2
1,867,545 + Pf6N2E2_1594 0.60 +0.3
1,867,545 + Pf6N2E2_1594 0.60 +0.2
1,867,545 + Pf6N2E2_1594 0.60 +0.1
1,867,546 - Pf6N2E2_1594 0.60 -0.5
1,867,546 - Pf6N2E2_1594 0.60 +1.6
1,867,546 - Pf6N2E2_1594 0.60 +1.6
1,867,711 - Pf6N2E2_1594 0.84 -0.2
1,867,779 + -0.4
1,867,812 + -0.5
1,867,821 + -1.3
1,867,821 + -0.2
1,867,821 + +0.3
1,867,821 + +1.6
1,867,821 + +0.6
1,867,952 + -0.6
1,867,953 - +0.8
1,867,991 - +0.3
1,868,162 - Pf6N2E2_1595 0.85 -0.6
1,868,162 - Pf6N2E2_1595 0.85 +1.6
1,868,211 - -1.8
1,868,243 - -0.5
1,868,243 - +1.6
1,868,243 - -0.2
1,868,259 + Pf6N2E2_1596 0.13 +0.3
1,868,260 - Pf6N2E2_1596 0.13 +0.6
1,868,761 + Pf6N2E2_1597 0.11 -0.2
1,868,761 + Pf6N2E2_1597 0.11 +0.2
1,868,761 + Pf6N2E2_1597 0.11 +0.3
1,868,761 + Pf6N2E2_1597 0.11 +0.6
1,868,762 - Pf6N2E2_1597 0.11 +0.7
1,868,762 - Pf6N2E2_1597 0.11 -0.4
1,869,116 + Pf6N2E2_1597 0.25 -0.6
1,869,117 - Pf6N2E2_1597 0.25 +1.6
1,869,204 - Pf6N2E2_1597 0.29 -0.0
1,869,296 - Pf6N2E2_1597 0.32 +0.1
1,869,296 - Pf6N2E2_1597 0.32 +0.0
1,869,296 - Pf6N2E2_1597 0.32 -0.7
1,869,296 - Pf6N2E2_1597 0.32 +0.4

Or see this region's nucleotide sequence