Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1517

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1516 and Pf6N2E2_1517 are separated by 130 nucleotidesPf6N2E2_1517 and Pf6N2E2_1518 are separated by 88 nucleotidesPf6N2E2_1518 and Pf6N2E2_1519 are separated by 331 nucleotides Pf6N2E2_1516 - transcriptional regulator, Crp/Fnr family, at 1,771,810 to 1,772,562 _1516 Pf6N2E2_1517 - Arginine/ornithine antiporter ArcD, at 1,772,693 to 1,774,186 _1517 Pf6N2E2_1518 - heat shock protein, HSP20 family, at 1,774,275 to 1,774,814 _1518 Pf6N2E2_1519 - Metallo-beta-lactamase family protein, RNA-specific, at 1,775,146 to 1,776,501 _1519 Position (kb) 1772 1773 1774 1775Strain fitness (log2 ratio) -2 -1 0 1at 1771.778 kb on + strandat 1771.779 kb on - strandat 1771.779 kb on - strandat 1771.779 kb on - strandat 1771.793 kb on + strandat 1771.794 kb on - strandat 1772.009 kb on - strand, within Pf6N2E2_1516at 1772.009 kb on - strand, within Pf6N2E2_1516at 1772.052 kb on - strand, within Pf6N2E2_1516at 1772.211 kb on - strand, within Pf6N2E2_1516at 1772.211 kb on - strand, within Pf6N2E2_1516at 1772.325 kb on - strand, within Pf6N2E2_1516at 1772.347 kb on - strand, within Pf6N2E2_1516at 1772.360 kb on - strand, within Pf6N2E2_1516at 1772.588 kb on + strandat 1772.713 kb on + strandat 1772.714 kb on - strandat 1773.077 kb on + strand, within Pf6N2E2_1517at 1773.078 kb on - strand, within Pf6N2E2_1517at 1773.108 kb on - strand, within Pf6N2E2_1517at 1773.108 kb on - strand, within Pf6N2E2_1517at 1773.133 kb on + strand, within Pf6N2E2_1517at 1773.133 kb on + strand, within Pf6N2E2_1517at 1773.136 kb on + strand, within Pf6N2E2_1517at 1773.137 kb on - strand, within Pf6N2E2_1517at 1773.414 kb on + strand, within Pf6N2E2_1517at 1773.415 kb on - strand, within Pf6N2E2_1517at 1773.415 kb on - strand, within Pf6N2E2_1517at 1773.415 kb on - strand, within Pf6N2E2_1517at 1773.415 kb on - strand, within Pf6N2E2_1517at 1773.821 kb on + strand, within Pf6N2E2_1517at 1773.866 kb on + strand, within Pf6N2E2_1517at 1773.866 kb on + strand, within Pf6N2E2_1517at 1773.884 kb on + strand, within Pf6N2E2_1517at 1773.884 kb on + strand, within Pf6N2E2_1517at 1773.884 kb on + strand, within Pf6N2E2_1517at 1773.918 kb on - strand, within Pf6N2E2_1517at 1773.996 kb on - strand, within Pf6N2E2_1517at 1774.014 kb on - strand, within Pf6N2E2_1517at 1774.038 kb on - strandat 1774.185 kb on - strandat 1774.662 kb on + strand, within Pf6N2E2_1518at 1774.873 kb on + strandat 1774.874 kb on - strandat 1775.039 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
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1,771,778 + -1.3
1,771,779 - -2.1
1,771,779 - -0.4
1,771,779 - -0.7
1,771,793 + -0.8
1,771,794 - +0.7
1,772,009 - Pf6N2E2_1516 0.26 -0.3
1,772,009 - Pf6N2E2_1516 0.26 +0.9
1,772,052 - Pf6N2E2_1516 0.32 -0.2
1,772,211 - Pf6N2E2_1516 0.53 -0.0
1,772,211 - Pf6N2E2_1516 0.53 -0.6
1,772,325 - Pf6N2E2_1516 0.68 -0.4
1,772,347 - Pf6N2E2_1516 0.71 -0.0
1,772,360 - Pf6N2E2_1516 0.73 +0.6
1,772,588 + +1.4
1,772,713 + +0.6
1,772,714 - +0.1
1,773,077 + Pf6N2E2_1517 0.26 -0.7
1,773,078 - Pf6N2E2_1517 0.26 +1.0
1,773,108 - Pf6N2E2_1517 0.28 -0.5
1,773,108 - Pf6N2E2_1517 0.28 +0.1
1,773,133 + Pf6N2E2_1517 0.29 +0.9
1,773,133 + Pf6N2E2_1517 0.29 -0.3
1,773,136 + Pf6N2E2_1517 0.30 -0.8
1,773,137 - Pf6N2E2_1517 0.30 +1.1
1,773,414 + Pf6N2E2_1517 0.48 +0.2
1,773,415 - Pf6N2E2_1517 0.48 +0.3
1,773,415 - Pf6N2E2_1517 0.48 +0.7
1,773,415 - Pf6N2E2_1517 0.48 -0.2
1,773,415 - Pf6N2E2_1517 0.48 -0.6
1,773,821 + Pf6N2E2_1517 0.76 +0.6
1,773,866 + Pf6N2E2_1517 0.79 +0.2
1,773,866 + Pf6N2E2_1517 0.79 -0.5
1,773,884 + Pf6N2E2_1517 0.80 -0.4
1,773,884 + Pf6N2E2_1517 0.80 -0.3
1,773,884 + Pf6N2E2_1517 0.80 +0.1
1,773,918 - Pf6N2E2_1517 0.82 +1.2
1,773,996 - Pf6N2E2_1517 0.87 -0.6
1,774,014 - Pf6N2E2_1517 0.88 -0.4
1,774,038 - -0.6
1,774,185 - -0.2
1,774,662 + Pf6N2E2_1518 0.72 +0.2
1,774,873 + -0.3
1,774,874 - +0.4
1,775,039 - +1.6

Or see this region's nucleotide sequence