Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1337 and Pf6N2E2_1338 are separated by 199 nucleotides Pf6N2E2_1338 and Pf6N2E2_1339 are separated by 181 nucleotides
Pf6N2E2_1337 - Cys regulon transcriptional activator CysB, at 1,572,865 to 1,573,776
_1337
Pf6N2E2_1338 - ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1), at 1,573,976 to 1,575,016
_1338
Pf6N2E2_1339 - Acetylornithine deacetylase (EC 3.5.1.16), at 1,575,198 to 1,576,382
_1339
Position (kb)
1573
1574
1575
1576 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1573.335 kb on + strand, within Pf6N2E2_1337 at 1573.335 kb on + strand, within Pf6N2E2_1337 at 1573.484 kb on - strand, within Pf6N2E2_1337 at 1573.624 kb on + strand, within Pf6N2E2_1337 at 1573.624 kb on + strand, within Pf6N2E2_1337 at 1573.624 kb on + strand, within Pf6N2E2_1337 at 1573.624 kb on + strand, within Pf6N2E2_1337 at 1573.625 kb on - strand, within Pf6N2E2_1337 at 1573.713 kb on + strand at 1573.742 kb on - strand at 1573.742 kb on - strand at 1573.815 kb on + strand at 1573.815 kb on + strand at 1573.820 kb on - strand at 1573.971 kb on + strand at 1573.971 kb on + strand at 1573.971 kb on + strand at 1573.971 kb on + strand at 1574.469 kb on + strand, within Pf6N2E2_1338 at 1574.469 kb on + strand, within Pf6N2E2_1338 at 1574.470 kb on - strand, within Pf6N2E2_1338 at 1574.470 kb on - strand, within Pf6N2E2_1338 at 1574.470 kb on - strand, within Pf6N2E2_1338 at 1574.470 kb on - strand, within Pf6N2E2_1338 at 1574.520 kb on + strand, within Pf6N2E2_1338 at 1574.521 kb on - strand, within Pf6N2E2_1338 at 1574.521 kb on - strand, within Pf6N2E2_1338 at 1574.619 kb on + strand, within Pf6N2E2_1338 at 1574.620 kb on - strand, within Pf6N2E2_1338 at 1574.643 kb on + strand, within Pf6N2E2_1338 at 1574.643 kb on + strand, within Pf6N2E2_1338 at 1574.643 kb on + strand, within Pf6N2E2_1338 at 1574.643 kb on + strand, within Pf6N2E2_1338 at 1574.644 kb on - strand, within Pf6N2E2_1338 at 1574.644 kb on - strand, within Pf6N2E2_1338 at 1574.682 kb on + strand, within Pf6N2E2_1338 at 1574.682 kb on + strand, within Pf6N2E2_1338 at 1574.683 kb on - strand, within Pf6N2E2_1338 at 1574.683 kb on - strand, within Pf6N2E2_1338 at 1574.683 kb on - strand, within Pf6N2E2_1338 at 1574.683 kb on - strand, within Pf6N2E2_1338 at 1574.683 kb on - strand, within Pf6N2E2_1338 at 1574.835 kb on + strand, within Pf6N2E2_1338 at 1574.835 kb on + strand, within Pf6N2E2_1338 at 1574.836 kb on - strand, within Pf6N2E2_1338 at 1574.887 kb on - strand, within Pf6N2E2_1338 at 1574.993 kb on - strand at 1575.082 kb on - strand at 1575.082 kb on - strand at 1575.085 kb on + strand at 1575.102 kb on - strand at 1575.223 kb on + strand at 1575.223 kb on + strand at 1575.224 kb on - strand at 1575.224 kb on - strand at 1575.224 kb on - strand at 1575.280 kb on + strand at 1575.280 kb on + strand at 1575.281 kb on - strand at 1575.539 kb on - strand, within Pf6N2E2_1339
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 1,573,335 + Pf6N2E2_1337 0.52 +0.6 1,573,335 + Pf6N2E2_1337 0.52 -2.5 1,573,484 - Pf6N2E2_1337 0.68 +0.0 1,573,624 + Pf6N2E2_1337 0.83 -1.6 1,573,624 + Pf6N2E2_1337 0.83 -0.0 1,573,624 + Pf6N2E2_1337 0.83 +0.3 1,573,624 + Pf6N2E2_1337 0.83 +1.2 1,573,625 - Pf6N2E2_1337 0.83 -0.2 1,573,713 + -0.6 1,573,742 - +0.2 1,573,742 - +0.8 1,573,815 + +0.7 1,573,815 + +0.3 1,573,820 - +1.6 1,573,971 + +0.1 1,573,971 + +0.3 1,573,971 + -0.7 1,573,971 + +0.6 1,574,469 + Pf6N2E2_1338 0.47 +1.6 1,574,469 + Pf6N2E2_1338 0.47 -0.0 1,574,470 - Pf6N2E2_1338 0.47 +0.4 1,574,470 - Pf6N2E2_1338 0.47 -0.8 1,574,470 - Pf6N2E2_1338 0.47 -0.1 1,574,470 - Pf6N2E2_1338 0.47 -0.1 1,574,520 + Pf6N2E2_1338 0.52 +0.6 1,574,521 - Pf6N2E2_1338 0.52 -0.8 1,574,521 - Pf6N2E2_1338 0.52 +2.6 1,574,619 + Pf6N2E2_1338 0.62 +0.0 1,574,620 - Pf6N2E2_1338 0.62 +1.2 1,574,643 + Pf6N2E2_1338 0.64 +1.6 1,574,643 + Pf6N2E2_1338 0.64 -0.4 1,574,643 + Pf6N2E2_1338 0.64 +0.6 1,574,643 + Pf6N2E2_1338 0.64 +0.0 1,574,644 - Pf6N2E2_1338 0.64 +1.0 1,574,644 - Pf6N2E2_1338 0.64 +0.3 1,574,682 + Pf6N2E2_1338 0.68 -1.0 1,574,682 + Pf6N2E2_1338 0.68 +0.4 1,574,683 - Pf6N2E2_1338 0.68 -0.4 1,574,683 - Pf6N2E2_1338 0.68 +0.4 1,574,683 - Pf6N2E2_1338 0.68 +0.3 1,574,683 - Pf6N2E2_1338 0.68 +1.6 1,574,683 - Pf6N2E2_1338 0.68 -0.2 1,574,835 + Pf6N2E2_1338 0.83 +0.8 1,574,835 + Pf6N2E2_1338 0.83 +0.0 1,574,836 - Pf6N2E2_1338 0.83 +1.0 1,574,887 - Pf6N2E2_1338 0.88 +1.3 1,574,993 - +1.6 1,575,082 - +0.9 1,575,082 - -0.8 1,575,085 + -0.6 1,575,102 - +0.5 1,575,223 + -0.8 1,575,223 + +2.6 1,575,224 - +0.0 1,575,224 - -1.4 1,575,224 - -0.9 1,575,280 + +0.8 1,575,280 + +0.2 1,575,281 - +0.0 1,575,539 - Pf6N2E2_1339 0.29 -1.2
Or see this region's nucleotide sequence