Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1338

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1337 and Pf6N2E2_1338 are separated by 199 nucleotidesPf6N2E2_1338 and Pf6N2E2_1339 are separated by 181 nucleotides Pf6N2E2_1337 - Cys regulon transcriptional activator CysB, at 1,572,865 to 1,573,776 _1337 Pf6N2E2_1338 - ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1), at 1,573,976 to 1,575,016 _1338 Pf6N2E2_1339 - Acetylornithine deacetylase (EC 3.5.1.16), at 1,575,198 to 1,576,382 _1339 Position (kb) 1573 1574 1575 1576Strain fitness (log2 ratio) -2 -1 0 1 2at 1573.335 kb on + strand, within Pf6N2E2_1337at 1573.335 kb on + strand, within Pf6N2E2_1337at 1573.484 kb on - strand, within Pf6N2E2_1337at 1573.624 kb on + strand, within Pf6N2E2_1337at 1573.624 kb on + strand, within Pf6N2E2_1337at 1573.624 kb on + strand, within Pf6N2E2_1337at 1573.624 kb on + strand, within Pf6N2E2_1337at 1573.625 kb on - strand, within Pf6N2E2_1337at 1573.713 kb on + strandat 1573.742 kb on - strandat 1573.742 kb on - strandat 1573.815 kb on + strandat 1573.815 kb on + strandat 1573.820 kb on - strandat 1573.971 kb on + strandat 1573.971 kb on + strandat 1573.971 kb on + strandat 1573.971 kb on + strandat 1574.469 kb on + strand, within Pf6N2E2_1338at 1574.469 kb on + strand, within Pf6N2E2_1338at 1574.470 kb on - strand, within Pf6N2E2_1338at 1574.470 kb on - strand, within Pf6N2E2_1338at 1574.470 kb on - strand, within Pf6N2E2_1338at 1574.470 kb on - strand, within Pf6N2E2_1338at 1574.520 kb on + strand, within Pf6N2E2_1338at 1574.521 kb on - strand, within Pf6N2E2_1338at 1574.521 kb on - strand, within Pf6N2E2_1338at 1574.619 kb on + strand, within Pf6N2E2_1338at 1574.620 kb on - strand, within Pf6N2E2_1338at 1574.643 kb on + strand, within Pf6N2E2_1338at 1574.643 kb on + strand, within Pf6N2E2_1338at 1574.643 kb on + strand, within Pf6N2E2_1338at 1574.643 kb on + strand, within Pf6N2E2_1338at 1574.644 kb on - strand, within Pf6N2E2_1338at 1574.644 kb on - strand, within Pf6N2E2_1338at 1574.682 kb on + strand, within Pf6N2E2_1338at 1574.682 kb on + strand, within Pf6N2E2_1338at 1574.683 kb on - strand, within Pf6N2E2_1338at 1574.683 kb on - strand, within Pf6N2E2_1338at 1574.683 kb on - strand, within Pf6N2E2_1338at 1574.683 kb on - strand, within Pf6N2E2_1338at 1574.683 kb on - strand, within Pf6N2E2_1338at 1574.835 kb on + strand, within Pf6N2E2_1338at 1574.835 kb on + strand, within Pf6N2E2_1338at 1574.836 kb on - strand, within Pf6N2E2_1338at 1574.887 kb on - strand, within Pf6N2E2_1338at 1574.993 kb on - strandat 1575.082 kb on - strandat 1575.082 kb on - strandat 1575.085 kb on + strandat 1575.102 kb on - strandat 1575.223 kb on + strandat 1575.223 kb on + strandat 1575.224 kb on - strandat 1575.224 kb on - strandat 1575.224 kb on - strandat 1575.280 kb on + strandat 1575.280 kb on + strandat 1575.281 kb on - strandat 1575.539 kb on - strand, within Pf6N2E2_1339

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
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1,573,335 + Pf6N2E2_1337 0.52 +0.6
1,573,335 + Pf6N2E2_1337 0.52 -2.5
1,573,484 - Pf6N2E2_1337 0.68 +0.0
1,573,624 + Pf6N2E2_1337 0.83 -1.6
1,573,624 + Pf6N2E2_1337 0.83 -0.0
1,573,624 + Pf6N2E2_1337 0.83 +0.3
1,573,624 + Pf6N2E2_1337 0.83 +1.2
1,573,625 - Pf6N2E2_1337 0.83 -0.2
1,573,713 + -0.6
1,573,742 - +0.2
1,573,742 - +0.8
1,573,815 + +0.7
1,573,815 + +0.3
1,573,820 - +1.6
1,573,971 + +0.1
1,573,971 + +0.3
1,573,971 + -0.7
1,573,971 + +0.6
1,574,469 + Pf6N2E2_1338 0.47 +1.6
1,574,469 + Pf6N2E2_1338 0.47 -0.0
1,574,470 - Pf6N2E2_1338 0.47 +0.4
1,574,470 - Pf6N2E2_1338 0.47 -0.8
1,574,470 - Pf6N2E2_1338 0.47 -0.1
1,574,470 - Pf6N2E2_1338 0.47 -0.1
1,574,520 + Pf6N2E2_1338 0.52 +0.6
1,574,521 - Pf6N2E2_1338 0.52 -0.8
1,574,521 - Pf6N2E2_1338 0.52 +2.6
1,574,619 + Pf6N2E2_1338 0.62 +0.0
1,574,620 - Pf6N2E2_1338 0.62 +1.2
1,574,643 + Pf6N2E2_1338 0.64 +1.6
1,574,643 + Pf6N2E2_1338 0.64 -0.4
1,574,643 + Pf6N2E2_1338 0.64 +0.6
1,574,643 + Pf6N2E2_1338 0.64 +0.0
1,574,644 - Pf6N2E2_1338 0.64 +1.0
1,574,644 - Pf6N2E2_1338 0.64 +0.3
1,574,682 + Pf6N2E2_1338 0.68 -1.0
1,574,682 + Pf6N2E2_1338 0.68 +0.4
1,574,683 - Pf6N2E2_1338 0.68 -0.4
1,574,683 - Pf6N2E2_1338 0.68 +0.4
1,574,683 - Pf6N2E2_1338 0.68 +0.3
1,574,683 - Pf6N2E2_1338 0.68 +1.6
1,574,683 - Pf6N2E2_1338 0.68 -0.2
1,574,835 + Pf6N2E2_1338 0.83 +0.8
1,574,835 + Pf6N2E2_1338 0.83 +0.0
1,574,836 - Pf6N2E2_1338 0.83 +1.0
1,574,887 - Pf6N2E2_1338 0.88 +1.3
1,574,993 - +1.6
1,575,082 - +0.9
1,575,082 - -0.8
1,575,085 + -0.6
1,575,102 - +0.5
1,575,223 + -0.8
1,575,223 + +2.6
1,575,224 - +0.0
1,575,224 - -1.4
1,575,224 - -0.9
1,575,280 + +0.8
1,575,280 + +0.2
1,575,281 - +0.0
1,575,539 - Pf6N2E2_1339 0.29 -1.2

Or see this region's nucleotide sequence