Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1296

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1295 and Pf6N2E2_1296 are separated by 31 nucleotidesPf6N2E2_1296 and Pf6N2E2_1297 are separated by 110 nucleotides Pf6N2E2_1295 - Sensor histidine kinase, at 1,529,947 to 1,531,308 _1295 Pf6N2E2_1296 - DNA-binding response regulator, LuxR family, at 1,531,340 to 1,531,972 _1296 Pf6N2E2_1297 - Nucleoside-diphosphate-sugar epimerases, at 1,532,083 to 1,532,952 _1297 Position (kb) 1531 1532Strain fitness (log2 ratio) -3 -2 -1 0 1at 1530.385 kb on + strand, within Pf6N2E2_1295at 1530.433 kb on + strand, within Pf6N2E2_1295at 1530.434 kb on - strand, within Pf6N2E2_1295at 1530.434 kb on - strand, within Pf6N2E2_1295at 1530.593 kb on + strand, within Pf6N2E2_1295at 1530.593 kb on + strand, within Pf6N2E2_1295at 1530.670 kb on - strand, within Pf6N2E2_1295at 1531.164 kb on - strand, within Pf6N2E2_1295at 1531.605 kb on - strand, within Pf6N2E2_1296at 1531.605 kb on - strand, within Pf6N2E2_1296at 1531.613 kb on + strand, within Pf6N2E2_1296at 1531.619 kb on + strand, within Pf6N2E2_1296at 1531.620 kb on - strand, within Pf6N2E2_1296at 1531.659 kb on + strand, within Pf6N2E2_1296at 1531.660 kb on - strand, within Pf6N2E2_1296at 1531.727 kb on + strand, within Pf6N2E2_1296at 1531.727 kb on + strand, within Pf6N2E2_1296at 1531.727 kb on + strand, within Pf6N2E2_1296at 1531.727 kb on + strand, within Pf6N2E2_1296at 1531.728 kb on - strand, within Pf6N2E2_1296at 1531.941 kb on - strandat 1532.025 kb on + strandat 1532.096 kb on + strandat 1532.944 kb on + strandat 1532.945 kb on - strandat 1532.953 kb on - strandat 1532.953 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
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1,530,385 + Pf6N2E2_1295 0.32 -3.0
1,530,433 + Pf6N2E2_1295 0.36 -3.2
1,530,434 - Pf6N2E2_1295 0.36 -2.5
1,530,434 - Pf6N2E2_1295 0.36 -3.2
1,530,593 + Pf6N2E2_1295 0.47 -0.4
1,530,593 + Pf6N2E2_1295 0.47 +0.1
1,530,670 - Pf6N2E2_1295 0.53 -0.4
1,531,164 - Pf6N2E2_1295 0.89 -1.9
1,531,605 - Pf6N2E2_1296 0.42 -3.2
1,531,605 - Pf6N2E2_1296 0.42 -1.5
1,531,613 + Pf6N2E2_1296 0.43 +1.6
1,531,619 + Pf6N2E2_1296 0.44 -2.4
1,531,620 - Pf6N2E2_1296 0.44 -2.5
1,531,659 + Pf6N2E2_1296 0.50 +0.6
1,531,660 - Pf6N2E2_1296 0.51 -2.9
1,531,727 + Pf6N2E2_1296 0.61 -2.7
1,531,727 + Pf6N2E2_1296 0.61 -2.2
1,531,727 + Pf6N2E2_1296 0.61 -2.1
1,531,727 + Pf6N2E2_1296 0.61 -2.4
1,531,728 - Pf6N2E2_1296 0.61 -2.1
1,531,941 - -2.1
1,532,025 + +0.4
1,532,096 + -0.1
1,532,944 + +1.6
1,532,945 - -1.5
1,532,953 - -0.1
1,532,953 - +1.2

Or see this region's nucleotide sequence