Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1292 and Pf6N2E2_1293 are separated by 332 nucleotides Pf6N2E2_1293 and Pf6N2E2_1294 are separated by 15 nucleotides Pf6N2E2_1294 and Pf6N2E2_1295 are separated by 314 nucleotides
Pf6N2E2_1292 - Methyl-accepting chemotaxis protein, at 1,525,230 to 1,527,026
_1292
Pf6N2E2_1293 - COG2879, Hypothetical small protein yjiX, at 1,527,359 to 1,527,556
_1293
Pf6N2E2_1294 - Carbon starvation protein A, at 1,527,572 to 1,529,632
_1294
Pf6N2E2_1295 - Sensor histidine kinase, at 1,529,947 to 1,531,308
_1295
Position (kb)
1527
1528
1529
1530 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1526.652 kb on + strand, within Pf6N2E2_1292 at 1526.652 kb on + strand, within Pf6N2E2_1292 at 1526.652 kb on + strand, within Pf6N2E2_1292 at 1526.653 kb on - strand, within Pf6N2E2_1292 at 1526.653 kb on - strand, within Pf6N2E2_1292 at 1526.653 kb on - strand, within Pf6N2E2_1292 at 1526.664 kb on + strand, within Pf6N2E2_1292 at 1526.763 kb on + strand, within Pf6N2E2_1292 at 1526.911 kb on - strand at 1526.911 kb on - strand at 1526.911 kb on - strand at 1526.950 kb on + strand at 1526.950 kb on + strand at 1527.053 kb on + strand at 1527.053 kb on + strand at 1527.053 kb on + strand at 1527.053 kb on + strand at 1527.053 kb on + strand at 1527.053 kb on + strand at 1527.525 kb on + strand, within Pf6N2E2_1293 at 1527.529 kb on + strand, within Pf6N2E2_1293 at 1527.529 kb on + strand, within Pf6N2E2_1293 at 1528.461 kb on + strand, within Pf6N2E2_1294 at 1528.789 kb on - strand, within Pf6N2E2_1294 at 1528.993 kb on + strand, within Pf6N2E2_1294 at 1529.475 kb on + strand at 1529.496 kb on + strand at 1529.678 kb on - strand at 1529.678 kb on - strand at 1529.678 kb on - strand at 1529.730 kb on + strand at 1529.731 kb on - strand at 1529.731 kb on - strand at 1529.731 kb on - strand at 1529.742 kb on + strand at 1529.743 kb on - strand at 1529.743 kb on - strand at 1529.745 kb on + strand at 1529.746 kb on - strand at 1529.748 kb on + strand at 1529.748 kb on + strand at 1529.748 kb on + strand at 1529.748 kb on + strand at 1529.748 kb on + strand at 1529.749 kb on - strand at 1529.752 kb on + strand at 1529.752 kb on + strand at 1529.752 kb on + strand at 1529.752 kb on + strand at 1529.753 kb on - strand at 1529.753 kb on - strand at 1529.753 kb on - strand at 1529.753 kb on - strand at 1529.753 kb on - strand at 1529.803 kb on + strand at 1529.815 kb on + strand at 1529.816 kb on - strand at 1529.876 kb on + strand at 1529.876 kb on + strand at 1529.900 kb on + strand at 1529.901 kb on - strand at 1529.901 kb on - strand at 1529.901 kb on - strand at 1529.942 kb on + strand at 1529.942 kb on + strand at 1529.942 kb on + strand at 1529.942 kb on + strand at 1529.942 kb on + strand at 1529.943 kb on - strand at 1529.943 kb on - strand at 1529.943 kb on - strand at 1529.943 kb on - strand at 1529.943 kb on - strand at 1530.112 kb on + strand, within Pf6N2E2_1295 at 1530.112 kb on + strand, within Pf6N2E2_1295 at 1530.113 kb on - strand, within Pf6N2E2_1295 at 1530.222 kb on - strand at 1530.242 kb on - strand, within Pf6N2E2_1295 at 1530.299 kb on - strand, within Pf6N2E2_1295 at 1530.309 kb on + strand, within Pf6N2E2_1295 at 1530.385 kb on + strand, within Pf6N2E2_1295 at 1530.433 kb on + strand, within Pf6N2E2_1295 at 1530.434 kb on - strand, within Pf6N2E2_1295 at 1530.434 kb on - strand, within Pf6N2E2_1295 at 1530.593 kb on + strand, within Pf6N2E2_1295 at 1530.593 kb on + strand, within Pf6N2E2_1295
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 1,526,652 + Pf6N2E2_1292 0.79 +0.2 1,526,652 + Pf6N2E2_1292 0.79 -0.5 1,526,652 + Pf6N2E2_1292 0.79 +0.1 1,526,653 - Pf6N2E2_1292 0.79 +0.1 1,526,653 - Pf6N2E2_1292 0.79 +0.2 1,526,653 - Pf6N2E2_1292 0.79 -0.7 1,526,664 + Pf6N2E2_1292 0.80 +0.3 1,526,763 + Pf6N2E2_1292 0.85 +0.4 1,526,911 - +1.1 1,526,911 - +0.3 1,526,911 - +2.0 1,526,950 + -1.5 1,526,950 + +0.7 1,527,053 + -0.3 1,527,053 + -0.9 1,527,053 + -1.5 1,527,053 + -0.1 1,527,053 + +0.4 1,527,053 + +0.5 1,527,525 + Pf6N2E2_1293 0.84 -3.7 1,527,529 + Pf6N2E2_1293 0.86 -2.5 1,527,529 + Pf6N2E2_1293 0.86 -0.6 1,528,461 + Pf6N2E2_1294 0.43 -2.4 1,528,789 - Pf6N2E2_1294 0.59 -2.1 1,528,993 + Pf6N2E2_1294 0.69 -3.2 1,529,475 + -2.2 1,529,496 + -2.4 1,529,678 - -0.9 1,529,678 - +1.4 1,529,678 - -2.5 1,529,730 + -3.5 1,529,731 - -1.8 1,529,731 - -0.9 1,529,731 - +1.5 1,529,742 + -2.4 1,529,743 - -0.5 1,529,743 - -3.4 1,529,745 + +0.6 1,529,746 - +0.6 1,529,748 + -3.0 1,529,748 + -0.8 1,529,748 + -2.1 1,529,748 + -1.3 1,529,748 + -3.2 1,529,749 - -0.9 1,529,752 + -2.7 1,529,752 + -0.7 1,529,752 + -2.3 1,529,752 + -2.0 1,529,753 - -0.9 1,529,753 - +0.6 1,529,753 - +0.1 1,529,753 - -0.2 1,529,753 - -0.5 1,529,803 + -0.6 1,529,815 + -0.5 1,529,816 - +0.6 1,529,876 + +0.2 1,529,876 + +0.4 1,529,900 + +0.3 1,529,901 - +1.6 1,529,901 - +0.3 1,529,901 - +0.1 1,529,942 + +1.6 1,529,942 + -0.2 1,529,942 + +0.1 1,529,942 + -0.7 1,529,942 + +1.6 1,529,943 - -0.9 1,529,943 - +1.6 1,529,943 - +0.6 1,529,943 - +0.3 1,529,943 - -1.7 1,530,112 + Pf6N2E2_1295 0.12 -3.0 1,530,112 + Pf6N2E2_1295 0.12 +0.4 1,530,113 - Pf6N2E2_1295 0.12 +1.6 1,530,222 - -0.4 1,530,242 - Pf6N2E2_1295 0.22 +1.6 1,530,299 - Pf6N2E2_1295 0.26 -1.7 1,530,309 + Pf6N2E2_1295 0.27 -1.7 1,530,385 + Pf6N2E2_1295 0.32 -3.0 1,530,433 + Pf6N2E2_1295 0.36 -3.2 1,530,434 - Pf6N2E2_1295 0.36 -2.5 1,530,434 - Pf6N2E2_1295 0.36 -3.2 1,530,593 + Pf6N2E2_1295 0.47 -0.4 1,530,593 + Pf6N2E2_1295 0.47 +0.1
Or see this region's nucleotide sequence