Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1113 and Pf6N2E2_1114 are separated by 567 nucleotides Pf6N2E2_1114 and Pf6N2E2_1115 overlap by 1 nucleotides Pf6N2E2_1115 and Pf6N2E2_1116 are separated by 150 nucleotides
Pf6N2E2_1113 - Transcriptional regulator, TetR family, at 1,305,354 to 1,305,905
_1113
Pf6N2E2_1114 - Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4), at 1,306,473 to 1,309,034
_1114
Pf6N2E2_1115 - Nitrite reductase [NAD(P)H] small subunit (EC 1.7.1.4), at 1,309,034 to 1,309,420
_1115
Pf6N2E2_1116 - Inosine-5'-monophosphate dehydrogenase (EC 1.1.1.205), at 1,309,571 to 1,310,011
_1116
Position (kb)
1306
1307
1308
1309
1310 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1305.496 kb on - strand, within Pf6N2E2_1113 at 1305.496 kb on - strand, within Pf6N2E2_1113 at 1305.594 kb on + strand, within Pf6N2E2_1113 at 1305.688 kb on - strand, within Pf6N2E2_1113 at 1306.010 kb on + strand at 1306.010 kb on + strand at 1306.011 kb on - strand at 1306.080 kb on + strand at 1306.349 kb on + strand at 1306.349 kb on + strand at 1306.349 kb on + strand at 1306.350 kb on - strand at 1306.626 kb on + strand at 1306.626 kb on + strand at 1306.627 kb on - strand at 1306.651 kb on - strand at 1306.701 kb on + strand at 1306.702 kb on - strand at 1306.798 kb on - strand, within Pf6N2E2_1114 at 1306.878 kb on + strand, within Pf6N2E2_1114 at 1306.879 kb on - strand, within Pf6N2E2_1114 at 1306.879 kb on - strand, within Pf6N2E2_1114 at 1307.319 kb on + strand, within Pf6N2E2_1114 at 1307.320 kb on - strand, within Pf6N2E2_1114 at 1307.320 kb on - strand, within Pf6N2E2_1114 at 1307.320 kb on - strand, within Pf6N2E2_1114 at 1307.320 kb on - strand, within Pf6N2E2_1114 at 1307.320 kb on - strand, within Pf6N2E2_1114 at 1307.380 kb on - strand, within Pf6N2E2_1114 at 1307.628 kb on + strand, within Pf6N2E2_1114 at 1307.628 kb on + strand, within Pf6N2E2_1114 at 1307.628 kb on + strand, within Pf6N2E2_1114 at 1307.628 kb on + strand, within Pf6N2E2_1114 at 1307.629 kb on - strand, within Pf6N2E2_1114 at 1307.629 kb on - strand, within Pf6N2E2_1114 at 1307.631 kb on + strand, within Pf6N2E2_1114 at 1307.631 kb on + strand, within Pf6N2E2_1114 at 1307.632 kb on - strand, within Pf6N2E2_1114 at 1307.632 kb on - strand, within Pf6N2E2_1114 at 1307.649 kb on + strand, within Pf6N2E2_1114 at 1307.649 kb on + strand, within Pf6N2E2_1114 at 1307.649 kb on + strand, within Pf6N2E2_1114 at 1307.649 kb on + strand, within Pf6N2E2_1114 at 1307.649 kb on + strand, within Pf6N2E2_1114 at 1307.650 kb on - strand, within Pf6N2E2_1114 at 1307.650 kb on - strand, within Pf6N2E2_1114 at 1307.650 kb on - strand, within Pf6N2E2_1114 at 1307.650 kb on - strand, within Pf6N2E2_1114 at 1307.650 kb on - strand, within Pf6N2E2_1114 at 1307.650 kb on - strand, within Pf6N2E2_1114 at 1307.976 kb on + strand, within Pf6N2E2_1114 at 1307.976 kb on + strand, within Pf6N2E2_1114 at 1307.977 kb on - strand, within Pf6N2E2_1114 at 1307.977 kb on - strand, within Pf6N2E2_1114 at 1307.977 kb on - strand, within Pf6N2E2_1114 at 1307.977 kb on - strand, within Pf6N2E2_1114 at 1308.155 kb on + strand, within Pf6N2E2_1114 at 1308.184 kb on - strand, within Pf6N2E2_1114 at 1308.262 kb on - strand, within Pf6N2E2_1114 at 1308.270 kb on + strand, within Pf6N2E2_1114 at 1308.271 kb on - strand, within Pf6N2E2_1114 at 1308.271 kb on - strand, within Pf6N2E2_1114 at 1308.483 kb on + strand, within Pf6N2E2_1114 at 1308.684 kb on + strand, within Pf6N2E2_1114 at 1308.708 kb on + strand, within Pf6N2E2_1114 at 1308.708 kb on + strand, within Pf6N2E2_1114 at 1308.708 kb on + strand, within Pf6N2E2_1114 at 1308.709 kb on - strand, within Pf6N2E2_1114 at 1308.709 kb on - strand, within Pf6N2E2_1114 at 1308.778 kb on - strand at 1308.778 kb on - strand at 1309.058 kb on - strand at 1309.058 kb on - strand at 1309.178 kb on + strand, within Pf6N2E2_1115 at 1309.179 kb on - strand, within Pf6N2E2_1115 at 1309.179 kb on - strand, within Pf6N2E2_1115 at 1309.208 kb on + strand, within Pf6N2E2_1115 at 1309.208 kb on + strand, within Pf6N2E2_1115 at 1309.307 kb on + strand, within Pf6N2E2_1115 at 1309.308 kb on - strand, within Pf6N2E2_1115 at 1309.308 kb on - strand, within Pf6N2E2_1115 at 1309.308 kb on - strand, within Pf6N2E2_1115 at 1309.308 kb on - strand, within Pf6N2E2_1115 at 1309.542 kb on + strand at 1309.543 kb on - strand at 1309.543 kb on - strand at 1309.549 kb on + strand at 1309.550 kb on - strand at 1309.566 kb on - strand at 1309.746 kb on - strand, within Pf6N2E2_1116 at 1309.804 kb on + strand, within Pf6N2E2_1116 at 1309.805 kb on - strand, within Pf6N2E2_1116 at 1309.994 kb on + strand at 1309.994 kb on + strand at 1309.994 kb on + strand at 1309.995 kb on - strand at 1309.995 kb on - strand at 1309.995 kb on - strand at 1309.995 kb on - strand at 1309.995 kb on - strand at 1309.995 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A remove 1,305,496 - Pf6N2E2_1113 0.26 +0.2 1,305,496 - Pf6N2E2_1113 0.26 +1.6 1,305,594 + Pf6N2E2_1113 0.43 -0.0 1,305,688 - Pf6N2E2_1113 0.61 +0.6 1,306,010 + -0.4 1,306,010 + -0.7 1,306,011 - +0.4 1,306,080 + -0.3 1,306,349 + -0.5 1,306,349 + -0.3 1,306,349 + -0.2 1,306,350 - +0.4 1,306,626 + -0.9 1,306,626 + +1.6 1,306,627 - -0.1 1,306,651 - +0.6 1,306,701 + -0.2 1,306,702 - -0.2 1,306,798 - Pf6N2E2_1114 0.13 -1.8 1,306,878 + Pf6N2E2_1114 0.16 -0.6 1,306,879 - Pf6N2E2_1114 0.16 +1.6 1,306,879 - Pf6N2E2_1114 0.16 -0.2 1,307,319 + Pf6N2E2_1114 0.33 -0.2 1,307,320 - Pf6N2E2_1114 0.33 +1.2 1,307,320 - Pf6N2E2_1114 0.33 -0.3 1,307,320 - Pf6N2E2_1114 0.33 +0.2 1,307,320 - Pf6N2E2_1114 0.33 -0.2 1,307,320 - Pf6N2E2_1114 0.33 +0.6 1,307,380 - Pf6N2E2_1114 0.35 +0.5 1,307,628 + Pf6N2E2_1114 0.45 +0.0 1,307,628 + Pf6N2E2_1114 0.45 +1.8 1,307,628 + Pf6N2E2_1114 0.45 +0.2 1,307,628 + Pf6N2E2_1114 0.45 +0.6 1,307,629 - Pf6N2E2_1114 0.45 -1.2 1,307,629 - Pf6N2E2_1114 0.45 +0.5 1,307,631 + Pf6N2E2_1114 0.45 +1.1 1,307,631 + Pf6N2E2_1114 0.45 +0.7 1,307,632 - Pf6N2E2_1114 0.45 +1.6 1,307,632 - Pf6N2E2_1114 0.45 +0.4 1,307,649 + Pf6N2E2_1114 0.46 +0.5 1,307,649 + Pf6N2E2_1114 0.46 +0.8 1,307,649 + Pf6N2E2_1114 0.46 -0.6 1,307,649 + Pf6N2E2_1114 0.46 +0.8 1,307,649 + Pf6N2E2_1114 0.46 +0.6 1,307,650 - Pf6N2E2_1114 0.46 -1.2 1,307,650 - Pf6N2E2_1114 0.46 +1.6 1,307,650 - Pf6N2E2_1114 0.46 +1.0 1,307,650 - Pf6N2E2_1114 0.46 +0.7 1,307,650 - Pf6N2E2_1114 0.46 +0.4 1,307,650 - Pf6N2E2_1114 0.46 -0.8 1,307,976 + Pf6N2E2_1114 0.59 -1.0 1,307,976 + Pf6N2E2_1114 0.59 +1.6 1,307,977 - Pf6N2E2_1114 0.59 +0.1 1,307,977 - Pf6N2E2_1114 0.59 -0.7 1,307,977 - Pf6N2E2_1114 0.59 +0.5 1,307,977 - Pf6N2E2_1114 0.59 -0.4 1,308,155 + Pf6N2E2_1114 0.66 -1.0 1,308,184 - Pf6N2E2_1114 0.67 -0.2 1,308,262 - Pf6N2E2_1114 0.70 -0.2 1,308,270 + Pf6N2E2_1114 0.70 -0.4 1,308,271 - Pf6N2E2_1114 0.70 +0.4 1,308,271 - Pf6N2E2_1114 0.70 +0.4 1,308,483 + Pf6N2E2_1114 0.78 +0.2 1,308,684 + Pf6N2E2_1114 0.86 +0.9 1,308,708 + Pf6N2E2_1114 0.87 -0.3 1,308,708 + Pf6N2E2_1114 0.87 +0.3 1,308,708 + Pf6N2E2_1114 0.87 +1.0 1,308,709 - Pf6N2E2_1114 0.87 -1.2 1,308,709 - Pf6N2E2_1114 0.87 +0.1 1,308,778 - -0.7 1,308,778 - -0.2 1,309,058 - +0.4 1,309,058 - +1.4 1,309,178 + Pf6N2E2_1115 0.37 +0.0 1,309,179 - Pf6N2E2_1115 0.37 -0.1 1,309,179 - Pf6N2E2_1115 0.37 +0.4 1,309,208 + Pf6N2E2_1115 0.45 +0.5 1,309,208 + Pf6N2E2_1115 0.45 +0.4 1,309,307 + Pf6N2E2_1115 0.71 +0.9 1,309,308 - Pf6N2E2_1115 0.71 -1.0 1,309,308 - Pf6N2E2_1115 0.71 -2.3 1,309,308 - Pf6N2E2_1115 0.71 +1.0 1,309,308 - Pf6N2E2_1115 0.71 +1.4 1,309,542 + +3.6 1,309,543 - -1.0 1,309,543 - -1.0 1,309,549 + -0.1 1,309,550 - +0.6 1,309,566 - +0.2 1,309,746 - Pf6N2E2_1116 0.40 -0.2 1,309,804 + Pf6N2E2_1116 0.53 -1.4 1,309,805 - Pf6N2E2_1116 0.53 +0.6 1,309,994 + +0.6 1,309,994 + +0.1 1,309,994 + +0.0 1,309,995 - -0.0 1,309,995 - -0.9 1,309,995 - -0.2 1,309,995 - +1.3 1,309,995 - +1.6 1,309,995 - +0.8
Or see this region's nucleotide sequence