Experiment: rhizosphere EcoFAB sample 3; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22310 and BWI76_RS22315 overlap by 1 nucleotides BWI76_RS22315 and BWI76_RS22320 are separated by 9 nucleotides BWI76_RS22320 and BWI76_RS22325 are separated by 17 nucleotides BWI76_RS22325 and BWI76_RS22330 are separated by 2 nucleotides
BWI76_RS22310: BWI76_RS22310 - hydrogenase 3 small subunit, at 4,650,832 to 4,651,599
_RS22310
BWI76_RS22315: BWI76_RS22315 - formate hydrogenlyase complex iron-sulfur subunit, at 4,651,599 to 4,652,141
_RS22315
BWI76_RS22320: BWI76_RS22320 - hydrogenase 3 large subunit, at 4,652,151 to 4,653,860
_RS22320
BWI76_RS22325: BWI76_RS22325 - hydrogenase 3 membrane subunit, at 4,653,878 to 4,654,801
_RS22325
BWI76_RS22330: BWI76_RS22330 - formate hydrogenlyase subunit 3, at 4,654,804 to 4,656,630
_RS22330
Position (kb) 4652
4653
4654 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4651.316 kb on + strand, within BWI76_RS22310 at 4651.334 kb on - strand, within BWI76_RS22310 at 4651.414 kb on - strand, within BWI76_RS22310 at 4651.414 kb on - strand, within BWI76_RS22310 at 4651.518 kb on + strand, within BWI76_RS22310 at 4651.620 kb on - strand at 4651.646 kb on + strand at 4651.656 kb on + strand, within BWI76_RS22315 at 4651.664 kb on - strand, within BWI76_RS22315 at 4651.664 kb on - strand, within BWI76_RS22315 at 4652.005 kb on - strand, within BWI76_RS22315 at 4652.078 kb on + strand, within BWI76_RS22315 at 4652.102 kb on - strand at 4652.190 kb on + strand at 4652.212 kb on + strand at 4652.234 kb on + strand at 4652.257 kb on + strand at 4652.257 kb on + strand at 4652.265 kb on - strand at 4652.281 kb on - strand at 4652.309 kb on + strand at 4652.392 kb on - strand, within BWI76_RS22320 at 4652.465 kb on + strand, within BWI76_RS22320 at 4652.472 kb on + strand, within BWI76_RS22320 at 4652.473 kb on - strand, within BWI76_RS22320 at 4652.542 kb on + strand, within BWI76_RS22320 at 4652.550 kb on - strand, within BWI76_RS22320 at 4652.550 kb on - strand, within BWI76_RS22320 at 4652.597 kb on - strand, within BWI76_RS22320 at 4652.794 kb on - strand, within BWI76_RS22320 at 4652.884 kb on - strand, within BWI76_RS22320 at 4652.932 kb on - strand, within BWI76_RS22320 at 4653.168 kb on + strand, within BWI76_RS22320 at 4653.222 kb on - strand, within BWI76_RS22320 at 4653.289 kb on + strand, within BWI76_RS22320 at 4653.314 kb on + strand, within BWI76_RS22320 at 4653.327 kb on - strand, within BWI76_RS22320 at 4653.353 kb on + strand, within BWI76_RS22320 at 4653.581 kb on + strand, within BWI76_RS22320 at 4653.607 kb on + strand, within BWI76_RS22320 at 4653.779 kb on + strand at 4653.956 kb on - strand at 4654.110 kb on + strand, within BWI76_RS22325 at 4654.128 kb on - strand, within BWI76_RS22325 at 4654.312 kb on - strand, within BWI76_RS22325 at 4654.328 kb on + strand, within BWI76_RS22325 at 4654.367 kb on - strand, within BWI76_RS22325 at 4654.576 kb on + strand, within BWI76_RS22325
Per-strain Table
Position Strand Gene LocusTag Fraction rhizosphere EcoFAB sample 3; outgrowth in LB remove 4,651,316 + BWI76_RS22310 0.63 -0.3 4,651,334 - BWI76_RS22310 0.65 +0.9 4,651,414 - BWI76_RS22310 0.76 -2.3 4,651,414 - BWI76_RS22310 0.76 -1.7 4,651,518 + BWI76_RS22310 0.89 +2.4 4,651,620 - +1.2 4,651,646 + +0.7 4,651,656 + BWI76_RS22315 0.10 +0.3 4,651,664 - BWI76_RS22315 0.12 +0.4 4,651,664 - BWI76_RS22315 0.12 +2.0 4,652,005 - BWI76_RS22315 0.75 +0.9 4,652,078 + BWI76_RS22315 0.88 -0.2 4,652,102 - -0.6 4,652,190 + -1.5 4,652,212 + +0.2 4,652,234 + -0.5 4,652,257 + +1.1 4,652,257 + -0.6 4,652,265 - +1.3 4,652,281 - +1.0 4,652,309 + -1.2 4,652,392 - BWI76_RS22320 0.14 +0.2 4,652,465 + BWI76_RS22320 0.18 -0.1 4,652,472 + BWI76_RS22320 0.19 +1.0 4,652,473 - BWI76_RS22320 0.19 +0.2 4,652,542 + BWI76_RS22320 0.23 -0.2 4,652,550 - BWI76_RS22320 0.23 +2.9 4,652,550 - BWI76_RS22320 0.23 -0.4 4,652,597 - BWI76_RS22320 0.26 +0.8 4,652,794 - BWI76_RS22320 0.38 +0.6 4,652,884 - BWI76_RS22320 0.43 -0.8 4,652,932 - BWI76_RS22320 0.46 -0.1 4,653,168 + BWI76_RS22320 0.59 +0.2 4,653,222 - BWI76_RS22320 0.63 +1.0 4,653,289 + BWI76_RS22320 0.67 +1.2 4,653,314 + BWI76_RS22320 0.68 +0.5 4,653,327 - BWI76_RS22320 0.69 +0.3 4,653,353 + BWI76_RS22320 0.70 +0.6 4,653,581 + BWI76_RS22320 0.84 +0.7 4,653,607 + BWI76_RS22320 0.85 -0.2 4,653,779 + -1.2 4,653,956 - -0.6 4,654,110 + BWI76_RS22325 0.25 +0.7 4,654,128 - BWI76_RS22325 0.27 -0.3 4,654,312 - BWI76_RS22325 0.47 +0.6 4,654,328 + BWI76_RS22325 0.49 +0.3 4,654,367 - BWI76_RS22325 0.53 -1.1 4,654,576 + BWI76_RS22325 0.76 -1.8
Or see this region's nucleotide sequence