Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS22240

Experiment: rhizosphere EcoFAB sample 3; outgrowth in LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS22230 and BWI76_RS22235 are separated by 133 nucleotidesBWI76_RS22235 and BWI76_RS22240 are separated by 68 nucleotidesBWI76_RS22240 and BWI76_RS22245 overlap by 8 nucleotidesBWI76_RS22245 and BWI76_RS22250 overlap by 38 nucleotides BWI76_RS22230: BWI76_RS22230 - Sorbitol-6-phosphate 2-dehydrogenase (EC 1.1.1.140) (from data), at 4,634,055 to 4,634,834 _RS22230 BWI76_RS22235: BWI76_RS22235 - transcriptional regulator GutM, at 4,634,968 to 4,635,327 _RS22235 BWI76_RS22240: BWI76_RS22240 - transcriptional regulator, at 4,635,396 to 4,636,169 _RS22240 BWI76_RS22245: BWI76_RS22245 - arabinose 5-phosphate isomerase GutQ, at 4,636,162 to 4,637,127 _RS22245 BWI76_RS22250: BWI76_RS22250 - nitric oxide reductase transcription regulator, at 4,637,090 to 4,638,634 _RS22250 Position (kb) 4635 4636 4637Strain fitness (log2 ratio) -7 -6 -5 -4 -3 -2 -1 0 1at 4634.454 kb on - strand, within BWI76_RS22230at 4634.508 kb on + strand, within BWI76_RS22230at 4634.581 kb on + strand, within BWI76_RS22230at 4634.664 kb on - strand, within BWI76_RS22230at 4634.679 kb on - strand, within BWI76_RS22230at 4635.182 kb on - strand, within BWI76_RS22235at 4635.467 kb on - strandat 4635.495 kb on - strand, within BWI76_RS22240at 4635.583 kb on - strand, within BWI76_RS22240at 4635.705 kb on + strand, within BWI76_RS22240at 4635.964 kb on - strand, within BWI76_RS22240at 4636.176 kb on + strandat 4636.196 kb on + strandat 4636.356 kb on - strand, within BWI76_RS22245at 4636.472 kb on - strand, within BWI76_RS22245at 4636.513 kb on - strand, within BWI76_RS22245at 4636.622 kb on + strand, within BWI76_RS22245at 4636.623 kb on + strand, within BWI76_RS22245at 4636.835 kb on - strand, within BWI76_RS22245at 4636.847 kb on + strand, within BWI76_RS22245at 4637.022 kb on - strand, within BWI76_RS22245at 4637.163 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction rhizosphere EcoFAB sample 3; outgrowth in LB
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4,634,454 - BWI76_RS22230 0.51 +0.8
4,634,508 + BWI76_RS22230 0.58 -0.4
4,634,581 + BWI76_RS22230 0.67 +0.8
4,634,664 - BWI76_RS22230 0.78 -1.1
4,634,679 - BWI76_RS22230 0.80 -0.4
4,635,182 - BWI76_RS22235 0.59 +0.8
4,635,467 - -2.1
4,635,495 - BWI76_RS22240 0.13 -5.2
4,635,583 - BWI76_RS22240 0.24 -4.8
4,635,705 + BWI76_RS22240 0.40 -4.2
4,635,964 - BWI76_RS22240 0.73 -7.5
4,636,176 + -0.2
4,636,196 + -1.3
4,636,356 - BWI76_RS22245 0.20 -1.1
4,636,472 - BWI76_RS22245 0.32 -0.1
4,636,513 - BWI76_RS22245 0.36 -3.2
4,636,622 + BWI76_RS22245 0.48 +0.0
4,636,623 + BWI76_RS22245 0.48 +0.1
4,636,835 - BWI76_RS22245 0.70 +0.9
4,636,847 + BWI76_RS22245 0.71 +0.6
4,637,022 - BWI76_RS22245 0.89 -2.3
4,637,163 - -0.2

Or see this region's nucleotide sequence