Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16940

Experiment: rhizosphere EcoFAB sample 3; outgrowth in LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16930 and BWI76_RS16935 are separated by 63 nucleotidesBWI76_RS16935 and BWI76_RS16940 are separated by 31 nucleotidesBWI76_RS16940 and BWI76_RS16945 are separated by 79 nucleotides BWI76_RS16930: BWI76_RS16930 - transcriptional regulator, at 3,511,058 to 3,511,864 _RS16930 BWI76_RS16935: BWI76_RS16935 - shikimate dehydrogenase, at 3,511,928 to 3,512,764 _RS16935 BWI76_RS16940: BWI76_RS16940 - MFS transporter, at 3,512,796 to 3,514,115 _RS16940 BWI76_RS16945: BWI76_RS16945 - 4-hydroxyphenylpyruvate dioxygenase, at 3,514,195 to 3,516,048 _RS16945 Position (kb) 3512 3513 3514 3515Strain fitness (log2 ratio) -1 0 1 2at 3511.957 kb on + strandat 3512.130 kb on - strand, within BWI76_RS16935at 3512.708 kb on - strandat 3513.503 kb on + strand, within BWI76_RS16940at 3513.684 kb on - strand, within BWI76_RS16940at 3513.762 kb on - strand, within BWI76_RS16940at 3513.906 kb on + strand, within BWI76_RS16940at 3513.956 kb on - strand, within BWI76_RS16940at 3513.981 kb on + strand, within BWI76_RS16940at 3514.156 kb on - strandat 3514.204 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction rhizosphere EcoFAB sample 3; outgrowth in LB
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3,511,957 + -1.3
3,512,130 - BWI76_RS16935 0.24 +1.1
3,512,708 - +1.9
3,513,503 + BWI76_RS16940 0.54 +1.3
3,513,684 - BWI76_RS16940 0.67 -0.4
3,513,762 - BWI76_RS16940 0.73 +0.8
3,513,906 + BWI76_RS16940 0.84 -0.2
3,513,956 - BWI76_RS16940 0.88 +0.3
3,513,981 + BWI76_RS16940 0.90 -0.4
3,514,156 - -0.0
3,514,204 + +0.6

Or see this region's nucleotide sequence