Experiment: rhizosphere EcoFAB sample 3; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10965 and BWI76_RS10970 overlap by 15 nucleotides BWI76_RS10970 and BWI76_RS10975 are separated by 73 nucleotides BWI76_RS10975 and BWI76_RS10980 are separated by 124 nucleotides BWI76_RS10980 and BWI76_RS10985 are separated by 285 nucleotides BWI76_RS10985 and BWI76_RS10990 are separated by 72 nucleotides
BWI76_RS10965: BWI76_RS10965 - sulfurtransferase, at 2,314,992 to 2,316,056
_RS10965
BWI76_RS10970: BWI76_RS10970 - DUF2770 domain-containing protein, at 2,316,042 to 2,316,155
_RS10970
BWI76_RS10975: BWI76_RS10975 - N-methyltryptophan oxidase, at 2,316,229 to 2,317,347
_RS10975
BWI76_RS10980: BWI76_RS10980 - biofilm formation regulatory protein BssS, at 2,317,472 to 2,317,726
_RS10980
BWI76_RS10985: BWI76_RS10985 - DNA damage-inducible protein I, at 2,318,012 to 2,318,257
_RS10985
BWI76_RS10990: BWI76_RS10990 - dihydroorotase, at 2,318,330 to 2,319,376
_RS10990
Position (kb)
2316
2317
2318 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2315.306 kb on + strand, within BWI76_RS10965 at 2315.320 kb on + strand, within BWI76_RS10965 at 2315.909 kb on - strand, within BWI76_RS10965 at 2316.539 kb on - strand, within BWI76_RS10975 at 2316.610 kb on - strand, within BWI76_RS10975 at 2317.096 kb on + strand, within BWI76_RS10975 at 2317.133 kb on + strand, within BWI76_RS10975 at 2317.198 kb on + strand, within BWI76_RS10975 at 2317.267 kb on + strand at 2317.274 kb on - strand at 2317.284 kb on + strand at 2317.284 kb on + strand at 2317.294 kb on + strand at 2317.337 kb on - strand at 2317.344 kb on + strand at 2317.352 kb on + strand at 2317.361 kb on - strand at 2317.367 kb on - strand at 2317.433 kb on - strand at 2317.492 kb on - strand at 2317.497 kb on - strand at 2317.517 kb on - strand, within BWI76_RS10980 at 2317.518 kb on - strand, within BWI76_RS10980 at 2317.638 kb on + strand, within BWI76_RS10980 at 2317.643 kb on + strand, within BWI76_RS10980 at 2317.670 kb on + strand, within BWI76_RS10980 at 2317.676 kb on - strand, within BWI76_RS10980 at 2317.680 kb on + strand, within BWI76_RS10980 at 2317.690 kb on + strand, within BWI76_RS10980 at 2317.697 kb on + strand, within BWI76_RS10980 at 2317.697 kb on + strand, within BWI76_RS10980 at 2317.698 kb on - strand, within BWI76_RS10980 at 2317.705 kb on - strand at 2317.705 kb on - strand at 2317.726 kb on + strand at 2317.927 kb on + strand at 2317.935 kb on - strand at 2317.945 kb on - strand at 2318.025 kb on + strand at 2318.033 kb on - strand at 2318.033 kb on - strand at 2318.035 kb on - strand at 2318.045 kb on - strand, within BWI76_RS10985 at 2318.134 kb on + strand, within BWI76_RS10985 at 2318.152 kb on - strand, within BWI76_RS10985 at 2318.193 kb on - strand, within BWI76_RS10985 at 2318.193 kb on - strand, within BWI76_RS10985
Per-strain Table
Position Strand Gene LocusTag Fraction rhizosphere EcoFAB sample 3; outgrowth in LB remove 2,315,306 + BWI76_RS10965 0.29 +0.3 2,315,320 + BWI76_RS10965 0.31 -0.1 2,315,909 - BWI76_RS10965 0.86 +0.2 2,316,539 - BWI76_RS10975 0.28 +0.0 2,316,610 - BWI76_RS10975 0.34 -1.0 2,317,096 + BWI76_RS10975 0.77 -2.7 2,317,133 + BWI76_RS10975 0.81 -1.2 2,317,198 + BWI76_RS10975 0.87 -0.2 2,317,267 + +0.3 2,317,274 - -0.6 2,317,284 + -0.8 2,317,284 + +0.1 2,317,294 + -1.3 2,317,337 - +0.1 2,317,344 + -0.5 2,317,352 + -1.0 2,317,361 - +1.0 2,317,367 - -2.6 2,317,433 - +0.2 2,317,492 - +0.2 2,317,497 - +0.8 2,317,517 - BWI76_RS10980 0.18 +0.6 2,317,518 - BWI76_RS10980 0.18 -0.2 2,317,638 + BWI76_RS10980 0.65 +1.6 2,317,643 + BWI76_RS10980 0.67 -1.7 2,317,670 + BWI76_RS10980 0.78 -1.8 2,317,676 - BWI76_RS10980 0.80 +0.7 2,317,680 + BWI76_RS10980 0.82 -3.5 2,317,690 + BWI76_RS10980 0.85 -0.1 2,317,697 + BWI76_RS10980 0.88 -2.9 2,317,697 + BWI76_RS10980 0.88 -0.8 2,317,698 - BWI76_RS10980 0.89 -0.4 2,317,705 - +0.0 2,317,705 - +0.6 2,317,726 + +0.4 2,317,927 + +0.8 2,317,935 - +0.0 2,317,945 - +1.2 2,318,025 + -0.5 2,318,033 - -0.4 2,318,033 - +1.2 2,318,035 - +2.8 2,318,045 - BWI76_RS10985 0.13 -0.2 2,318,134 + BWI76_RS10985 0.50 -0.2 2,318,152 - BWI76_RS10985 0.57 +1.3 2,318,193 - BWI76_RS10985 0.74 +1.1 2,318,193 - BWI76_RS10985 0.74 -0.0
Or see this region's nucleotide sequence