Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10975

Experiment: rhizosphere EcoFAB sample 3; outgrowth in LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10965 and BWI76_RS10970 overlap by 15 nucleotidesBWI76_RS10970 and BWI76_RS10975 are separated by 73 nucleotidesBWI76_RS10975 and BWI76_RS10980 are separated by 124 nucleotidesBWI76_RS10980 and BWI76_RS10985 are separated by 285 nucleotidesBWI76_RS10985 and BWI76_RS10990 are separated by 72 nucleotides BWI76_RS10965: BWI76_RS10965 - sulfurtransferase, at 2,314,992 to 2,316,056 _RS10965 BWI76_RS10970: BWI76_RS10970 - DUF2770 domain-containing protein, at 2,316,042 to 2,316,155 _RS10970 BWI76_RS10975: BWI76_RS10975 - N-methyltryptophan oxidase, at 2,316,229 to 2,317,347 _RS10975 BWI76_RS10980: BWI76_RS10980 - biofilm formation regulatory protein BssS, at 2,317,472 to 2,317,726 _RS10980 BWI76_RS10985: BWI76_RS10985 - DNA damage-inducible protein I, at 2,318,012 to 2,318,257 _RS10985 BWI76_RS10990: BWI76_RS10990 - dihydroorotase, at 2,318,330 to 2,319,376 _RS10990 Position (kb) 2316 2317 2318Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2315.306 kb on + strand, within BWI76_RS10965at 2315.320 kb on + strand, within BWI76_RS10965at 2315.909 kb on - strand, within BWI76_RS10965at 2316.539 kb on - strand, within BWI76_RS10975at 2316.610 kb on - strand, within BWI76_RS10975at 2317.096 kb on + strand, within BWI76_RS10975at 2317.133 kb on + strand, within BWI76_RS10975at 2317.198 kb on + strand, within BWI76_RS10975at 2317.267 kb on + strandat 2317.274 kb on - strandat 2317.284 kb on + strandat 2317.284 kb on + strandat 2317.294 kb on + strandat 2317.337 kb on - strandat 2317.344 kb on + strandat 2317.352 kb on + strandat 2317.361 kb on - strandat 2317.367 kb on - strandat 2317.433 kb on - strandat 2317.492 kb on - strandat 2317.497 kb on - strandat 2317.517 kb on - strand, within BWI76_RS10980at 2317.518 kb on - strand, within BWI76_RS10980at 2317.638 kb on + strand, within BWI76_RS10980at 2317.643 kb on + strand, within BWI76_RS10980at 2317.670 kb on + strand, within BWI76_RS10980at 2317.676 kb on - strand, within BWI76_RS10980at 2317.680 kb on + strand, within BWI76_RS10980at 2317.690 kb on + strand, within BWI76_RS10980at 2317.697 kb on + strand, within BWI76_RS10980at 2317.697 kb on + strand, within BWI76_RS10980at 2317.698 kb on - strand, within BWI76_RS10980at 2317.705 kb on - strandat 2317.705 kb on - strandat 2317.726 kb on + strandat 2317.927 kb on + strandat 2317.935 kb on - strandat 2317.945 kb on - strandat 2318.025 kb on + strandat 2318.033 kb on - strandat 2318.033 kb on - strandat 2318.035 kb on - strandat 2318.045 kb on - strand, within BWI76_RS10985at 2318.134 kb on + strand, within BWI76_RS10985at 2318.152 kb on - strand, within BWI76_RS10985at 2318.193 kb on - strand, within BWI76_RS10985at 2318.193 kb on - strand, within BWI76_RS10985

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Per-strain Table

Position Strand Gene LocusTag Fraction rhizosphere EcoFAB sample 3; outgrowth in LB
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2,315,306 + BWI76_RS10965 0.29 +0.3
2,315,320 + BWI76_RS10965 0.31 -0.1
2,315,909 - BWI76_RS10965 0.86 +0.2
2,316,539 - BWI76_RS10975 0.28 +0.0
2,316,610 - BWI76_RS10975 0.34 -1.0
2,317,096 + BWI76_RS10975 0.77 -2.7
2,317,133 + BWI76_RS10975 0.81 -1.2
2,317,198 + BWI76_RS10975 0.87 -0.2
2,317,267 + +0.3
2,317,274 - -0.6
2,317,284 + -0.8
2,317,284 + +0.1
2,317,294 + -1.3
2,317,337 - +0.1
2,317,344 + -0.5
2,317,352 + -1.0
2,317,361 - +1.0
2,317,367 - -2.6
2,317,433 - +0.2
2,317,492 - +0.2
2,317,497 - +0.8
2,317,517 - BWI76_RS10980 0.18 +0.6
2,317,518 - BWI76_RS10980 0.18 -0.2
2,317,638 + BWI76_RS10980 0.65 +1.6
2,317,643 + BWI76_RS10980 0.67 -1.7
2,317,670 + BWI76_RS10980 0.78 -1.8
2,317,676 - BWI76_RS10980 0.80 +0.7
2,317,680 + BWI76_RS10980 0.82 -3.5
2,317,690 + BWI76_RS10980 0.85 -0.1
2,317,697 + BWI76_RS10980 0.88 -2.9
2,317,697 + BWI76_RS10980 0.88 -0.8
2,317,698 - BWI76_RS10980 0.89 -0.4
2,317,705 - +0.0
2,317,705 - +0.6
2,317,726 + +0.4
2,317,927 + +0.8
2,317,935 - +0.0
2,317,945 - +1.2
2,318,025 + -0.5
2,318,033 - -0.4
2,318,033 - +1.2
2,318,035 - +2.8
2,318,045 - BWI76_RS10985 0.13 -0.2
2,318,134 + BWI76_RS10985 0.50 -0.2
2,318,152 - BWI76_RS10985 0.57 +1.3
2,318,193 - BWI76_RS10985 0.74 +1.1
2,318,193 - BWI76_RS10985 0.74 -0.0

Or see this region's nucleotide sequence