Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS09125

Experiment: rhizosphere EcoFAB sample 3; outgrowth in LB

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntBWI76_RS09115 and BWI76_RS09120 are separated by 259 nucleotidesBWI76_RS09120 and BWI76_RS09125 are separated by 33 nucleotidesBWI76_RS09125 and BWI76_RS09130 are separated by 376 nucleotidesBWI76_RS09130 and BWI76_RS09135 are separated by 104 nucleotides BWI76_RS09115: BWI76_RS09115 - 30S ribosomal protein S12 methylthiotransferase RimO, at 1,925,715 to 1,927,040 _RS09115 BWI76_RS09120: BWI76_RS09120 - hypothetical protein, at 1,927,300 to 1,927,818 _RS09120 BWI76_RS09125: BWI76_RS09125 - MFS transporter, at 1,927,852 to 1,929,096 _RS09125 BWI76_RS09130: BWI76_RS09130 - biofilm formation regulatory protein BssR, at 1,929,473 to 1,929,856 _RS09130 BWI76_RS09135: BWI76_RS09135 - oxidoreductase, at 1,929,961 to 1,931,073 _RS09135 Position (kb) 1927 1928 1929 1930Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1926.855 kb on + strand, within BWI76_RS09115at 1927.001 kb on - strandat 1927.069 kb on + strandat 1927.077 kb on - strandat 1927.082 kb on - strandat 1927.111 kb on - strandat 1927.113 kb on + strandat 1927.228 kb on + strandat 1927.228 kb on + strandat 1927.228 kb on + strandat 1927.236 kb on - strandat 1927.236 kb on - strandat 1927.238 kb on + strandat 1927.238 kb on + strandat 1927.241 kb on - strandat 1927.246 kb on - strandat 1927.318 kb on + strandat 1927.333 kb on - strandat 1927.343 kb on - strandat 1927.383 kb on - strandat 1927.386 kb on + strand, within BWI76_RS09120at 1927.472 kb on + strand, within BWI76_RS09120at 1927.472 kb on + strand, within BWI76_RS09120at 1927.472 kb on + strand, within BWI76_RS09120at 1927.498 kb on - strand, within BWI76_RS09120at 1927.509 kb on + strand, within BWI76_RS09120at 1927.517 kb on - strand, within BWI76_RS09120at 1927.517 kb on - strand, within BWI76_RS09120at 1927.542 kb on + strand, within BWI76_RS09120at 1927.548 kb on + strand, within BWI76_RS09120at 1927.548 kb on + strand, within BWI76_RS09120at 1927.596 kb on + strand, within BWI76_RS09120at 1927.604 kb on - strand, within BWI76_RS09120at 1927.606 kb on + strand, within BWI76_RS09120at 1927.675 kb on - strand, within BWI76_RS09120at 1927.796 kb on + strandat 1927.866 kb on - strandat 1927.881 kb on + strandat 1927.902 kb on + strandat 1927.928 kb on + strandat 1927.945 kb on + strandat 1927.945 kb on + strandat 1927.973 kb on - strandat 1928.318 kb on - strand, within BWI76_RS09125at 1928.372 kb on + strand, within BWI76_RS09125at 1928.469 kb on + strand, within BWI76_RS09125at 1928.469 kb on + strand, within BWI76_RS09125at 1928.477 kb on - strand, within BWI76_RS09125at 1928.477 kb on - strand, within BWI76_RS09125at 1928.477 kb on - strand, within BWI76_RS09125at 1928.541 kb on + strand, within BWI76_RS09125at 1928.541 kb on + strand, within BWI76_RS09125at 1928.549 kb on - strand, within BWI76_RS09125at 1928.549 kb on - strand, within BWI76_RS09125at 1928.549 kb on - strand, within BWI76_RS09125at 1928.591 kb on - strand, within BWI76_RS09125at 1928.765 kb on - strand, within BWI76_RS09125at 1928.799 kb on - strand, within BWI76_RS09125at 1928.845 kb on - strand, within BWI76_RS09125at 1928.948 kb on + strand, within BWI76_RS09125at 1928.953 kb on + strand, within BWI76_RS09125at 1929.039 kb on - strandat 1929.105 kb on + strandat 1929.126 kb on - strandat 1929.476 kb on + strandat 1929.495 kb on - strandat 1929.619 kb on + strand, within BWI76_RS09130at 1929.740 kb on + strand, within BWI76_RS09130at 1929.748 kb on - strand, within BWI76_RS09130at 1929.929 kb on - strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction rhizosphere EcoFAB sample 3; outgrowth in LB
remove
1,926,855 + BWI76_RS09115 0.86 +0.1
1,927,001 - -0.3
1,927,069 + -1.3
1,927,077 - -1.1
1,927,082 - -3.0
1,927,111 - +0.9
1,927,113 + +0.7
1,927,228 + +0.0
1,927,228 + -0.8
1,927,228 + -1.3
1,927,236 - -1.9
1,927,236 - -2.9
1,927,238 + +0.2
1,927,238 + +0.1
1,927,241 - -1.3
1,927,246 - -2.2
1,927,318 + +0.6
1,927,333 - -0.9
1,927,343 - -1.9
1,927,383 - +0.6
1,927,386 + BWI76_RS09120 0.17 -0.1
1,927,472 + BWI76_RS09120 0.33 +0.2
1,927,472 + BWI76_RS09120 0.33 +0.4
1,927,472 + BWI76_RS09120 0.33 -1.8
1,927,498 - BWI76_RS09120 0.38 -0.6
1,927,509 + BWI76_RS09120 0.40 -1.0
1,927,517 - BWI76_RS09120 0.42 -2.0
1,927,517 - BWI76_RS09120 0.42 +2.7
1,927,542 + BWI76_RS09120 0.47 -0.3
1,927,548 + BWI76_RS09120 0.48 +0.3
1,927,548 + BWI76_RS09120 0.48 +0.0
1,927,596 + BWI76_RS09120 0.57 +0.4
1,927,604 - BWI76_RS09120 0.59 +0.5
1,927,606 + BWI76_RS09120 0.59 -2.7
1,927,675 - BWI76_RS09120 0.72 +0.2
1,927,796 + +1.4
1,927,866 - +0.8
1,927,881 + -0.2
1,927,902 + -1.9
1,927,928 + -0.0
1,927,945 + +1.0
1,927,945 + +0.3
1,927,973 - -0.7
1,928,318 - BWI76_RS09125 0.37 +0.6
1,928,372 + BWI76_RS09125 0.42 +0.5
1,928,469 + BWI76_RS09125 0.50 +1.3
1,928,469 + BWI76_RS09125 0.50 +0.5
1,928,477 - BWI76_RS09125 0.50 +0.5
1,928,477 - BWI76_RS09125 0.50 +1.6
1,928,477 - BWI76_RS09125 0.50 -0.0
1,928,541 + BWI76_RS09125 0.55 -1.2
1,928,541 + BWI76_RS09125 0.55 +1.6
1,928,549 - BWI76_RS09125 0.56 -0.9
1,928,549 - BWI76_RS09125 0.56 -2.0
1,928,549 - BWI76_RS09125 0.56 -0.1
1,928,591 - BWI76_RS09125 0.59 -0.2
1,928,765 - BWI76_RS09125 0.73 +0.6
1,928,799 - BWI76_RS09125 0.76 +0.2
1,928,845 - BWI76_RS09125 0.80 -2.9
1,928,948 + BWI76_RS09125 0.88 +3.1
1,928,953 + BWI76_RS09125 0.88 +0.5
1,929,039 - -0.7
1,929,105 + +1.5
1,929,126 - +0.7
1,929,476 + -0.3
1,929,495 - +0.8
1,929,619 + BWI76_RS09130 0.38 +0.2
1,929,740 + BWI76_RS09130 0.70 +0.4
1,929,748 - BWI76_RS09130 0.72 -0.3
1,929,929 - +0.3

Or see this region's nucleotide sequence