Experiment: rhizosphere EcoFAB sample 3; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09115 and BWI76_RS09120 are separated by 259 nucleotides BWI76_RS09120 and BWI76_RS09125 are separated by 33 nucleotides BWI76_RS09125 and BWI76_RS09130 are separated by 376 nucleotides BWI76_RS09130 and BWI76_RS09135 are separated by 104 nucleotides
BWI76_RS09115: BWI76_RS09115 - 30S ribosomal protein S12 methylthiotransferase RimO, at 1,925,715 to 1,927,040
_RS09115
BWI76_RS09120: BWI76_RS09120 - hypothetical protein, at 1,927,300 to 1,927,818
_RS09120
BWI76_RS09125: BWI76_RS09125 - MFS transporter, at 1,927,852 to 1,929,096
_RS09125
BWI76_RS09130: BWI76_RS09130 - biofilm formation regulatory protein BssR, at 1,929,473 to 1,929,856
_RS09130
BWI76_RS09135: BWI76_RS09135 - oxidoreductase, at 1,929,961 to 1,931,073
_RS09135
Position (kb)
1927
1928
1929
1930 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1926.855 kb on + strand, within BWI76_RS09115 at 1927.001 kb on - strand at 1927.069 kb on + strand at 1927.077 kb on - strand at 1927.082 kb on - strand at 1927.111 kb on - strand at 1927.113 kb on + strand at 1927.228 kb on + strand at 1927.228 kb on + strand at 1927.228 kb on + strand at 1927.236 kb on - strand at 1927.236 kb on - strand at 1927.238 kb on + strand at 1927.238 kb on + strand at 1927.241 kb on - strand at 1927.246 kb on - strand at 1927.318 kb on + strand at 1927.333 kb on - strand at 1927.343 kb on - strand at 1927.383 kb on - strand at 1927.386 kb on + strand, within BWI76_RS09120 at 1927.472 kb on + strand, within BWI76_RS09120 at 1927.472 kb on + strand, within BWI76_RS09120 at 1927.472 kb on + strand, within BWI76_RS09120 at 1927.498 kb on - strand, within BWI76_RS09120 at 1927.509 kb on + strand, within BWI76_RS09120 at 1927.517 kb on - strand, within BWI76_RS09120 at 1927.517 kb on - strand, within BWI76_RS09120 at 1927.542 kb on + strand, within BWI76_RS09120 at 1927.548 kb on + strand, within BWI76_RS09120 at 1927.548 kb on + strand, within BWI76_RS09120 at 1927.596 kb on + strand, within BWI76_RS09120 at 1927.604 kb on - strand, within BWI76_RS09120 at 1927.606 kb on + strand, within BWI76_RS09120 at 1927.675 kb on - strand, within BWI76_RS09120 at 1927.796 kb on + strand at 1927.866 kb on - strand at 1927.881 kb on + strand at 1927.902 kb on + strand at 1927.928 kb on + strand at 1927.945 kb on + strand at 1927.945 kb on + strand at 1927.973 kb on - strand at 1928.318 kb on - strand, within BWI76_RS09125 at 1928.372 kb on + strand, within BWI76_RS09125 at 1928.469 kb on + strand, within BWI76_RS09125 at 1928.469 kb on + strand, within BWI76_RS09125 at 1928.477 kb on - strand, within BWI76_RS09125 at 1928.477 kb on - strand, within BWI76_RS09125 at 1928.477 kb on - strand, within BWI76_RS09125 at 1928.541 kb on + strand, within BWI76_RS09125 at 1928.541 kb on + strand, within BWI76_RS09125 at 1928.549 kb on - strand, within BWI76_RS09125 at 1928.549 kb on - strand, within BWI76_RS09125 at 1928.549 kb on - strand, within BWI76_RS09125 at 1928.591 kb on - strand, within BWI76_RS09125 at 1928.765 kb on - strand, within BWI76_RS09125 at 1928.799 kb on - strand, within BWI76_RS09125 at 1928.845 kb on - strand, within BWI76_RS09125 at 1928.948 kb on + strand, within BWI76_RS09125 at 1928.953 kb on + strand, within BWI76_RS09125 at 1929.039 kb on - strand at 1929.105 kb on + strand at 1929.126 kb on - strand at 1929.476 kb on + strand at 1929.495 kb on - strand at 1929.619 kb on + strand, within BWI76_RS09130 at 1929.740 kb on + strand, within BWI76_RS09130 at 1929.748 kb on - strand, within BWI76_RS09130 at 1929.929 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction rhizosphere EcoFAB sample 3; outgrowth in LB remove 1,926,855 + BWI76_RS09115 0.86 +0.1 1,927,001 - -0.3 1,927,069 + -1.3 1,927,077 - -1.1 1,927,082 - -3.0 1,927,111 - +0.9 1,927,113 + +0.7 1,927,228 + +0.0 1,927,228 + -0.8 1,927,228 + -1.3 1,927,236 - -1.9 1,927,236 - -2.9 1,927,238 + +0.2 1,927,238 + +0.1 1,927,241 - -1.3 1,927,246 - -2.2 1,927,318 + +0.6 1,927,333 - -0.9 1,927,343 - -1.9 1,927,383 - +0.6 1,927,386 + BWI76_RS09120 0.17 -0.1 1,927,472 + BWI76_RS09120 0.33 +0.2 1,927,472 + BWI76_RS09120 0.33 +0.4 1,927,472 + BWI76_RS09120 0.33 -1.8 1,927,498 - BWI76_RS09120 0.38 -0.6 1,927,509 + BWI76_RS09120 0.40 -1.0 1,927,517 - BWI76_RS09120 0.42 -2.0 1,927,517 - BWI76_RS09120 0.42 +2.7 1,927,542 + BWI76_RS09120 0.47 -0.3 1,927,548 + BWI76_RS09120 0.48 +0.3 1,927,548 + BWI76_RS09120 0.48 +0.0 1,927,596 + BWI76_RS09120 0.57 +0.4 1,927,604 - BWI76_RS09120 0.59 +0.5 1,927,606 + BWI76_RS09120 0.59 -2.7 1,927,675 - BWI76_RS09120 0.72 +0.2 1,927,796 + +1.4 1,927,866 - +0.8 1,927,881 + -0.2 1,927,902 + -1.9 1,927,928 + -0.0 1,927,945 + +1.0 1,927,945 + +0.3 1,927,973 - -0.7 1,928,318 - BWI76_RS09125 0.37 +0.6 1,928,372 + BWI76_RS09125 0.42 +0.5 1,928,469 + BWI76_RS09125 0.50 +1.3 1,928,469 + BWI76_RS09125 0.50 +0.5 1,928,477 - BWI76_RS09125 0.50 +0.5 1,928,477 - BWI76_RS09125 0.50 +1.6 1,928,477 - BWI76_RS09125 0.50 -0.0 1,928,541 + BWI76_RS09125 0.55 -1.2 1,928,541 + BWI76_RS09125 0.55 +1.6 1,928,549 - BWI76_RS09125 0.56 -0.9 1,928,549 - BWI76_RS09125 0.56 -2.0 1,928,549 - BWI76_RS09125 0.56 -0.1 1,928,591 - BWI76_RS09125 0.59 -0.2 1,928,765 - BWI76_RS09125 0.73 +0.6 1,928,799 - BWI76_RS09125 0.76 +0.2 1,928,845 - BWI76_RS09125 0.80 -2.9 1,928,948 + BWI76_RS09125 0.88 +3.1 1,928,953 + BWI76_RS09125 0.88 +0.5 1,929,039 - -0.7 1,929,105 + +1.5 1,929,126 - +0.7 1,929,476 + -0.3 1,929,495 - +0.8 1,929,619 + BWI76_RS09130 0.38 +0.2 1,929,740 + BWI76_RS09130 0.70 +0.4 1,929,748 - BWI76_RS09130 0.72 -0.3 1,929,929 - +0.3
Or see this region's nucleotide sequence