Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04865

Experiment: rhizosphere EcoFAB sample 3; outgrowth in LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04855 and BWI76_RS04860 are separated by 31 nucleotidesBWI76_RS04860 and BWI76_RS04865 overlap by 1 nucleotidesBWI76_RS04865 and BWI76_RS04870 are separated by 176 nucleotides BWI76_RS04855: BWI76_RS04855 - regulatory protein AmpE, at 1,013,665 to 1,014,519 _RS04855 BWI76_RS04860: BWI76_RS04860 - alpha-N-arabinofuranosidase, at 1,014,551 to 1,015,501 _RS04860 BWI76_RS04865: BWI76_RS04865 - MFS transporter, at 1,015,501 to 1,016,907 _RS04865 BWI76_RS04870: BWI76_RS04870 - aromatic amino acid transporter AroP, at 1,017,084 to 1,018,451 _RS04870 Position (kb) 1015 1016 1017Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1014.681 kb on + strand, within BWI76_RS04860at 1014.735 kb on + strand, within BWI76_RS04860at 1014.748 kb on - strand, within BWI76_RS04860at 1014.824 kb on - strand, within BWI76_RS04860at 1014.841 kb on - strand, within BWI76_RS04860at 1015.293 kb on - strand, within BWI76_RS04860at 1015.293 kb on - strand, within BWI76_RS04860at 1015.366 kb on + strand, within BWI76_RS04860at 1015.374 kb on - strand, within BWI76_RS04860at 1015.400 kb on + strand, within BWI76_RS04860at 1015.493 kb on + strandat 1015.562 kb on - strandat 1015.683 kb on + strand, within BWI76_RS04865at 1015.683 kb on - strand, within BWI76_RS04865at 1015.885 kb on + strand, within BWI76_RS04865at 1015.893 kb on - strandat 1015.893 kb on - strand, within BWI76_RS04865at 1015.893 kb on - strand, within BWI76_RS04865at 1015.893 kb on - strand, within BWI76_RS04865at 1016.033 kb on - strand, within BWI76_RS04865at 1016.093 kb on - strand, within BWI76_RS04865at 1016.093 kb on - strand, within BWI76_RS04865at 1016.132 kb on + strand, within BWI76_RS04865at 1016.149 kb on - strand, within BWI76_RS04865at 1016.274 kb on - strandat 1016.367 kb on - strand, within BWI76_RS04865at 1016.380 kb on - strand, within BWI76_RS04865at 1016.535 kb on + strand, within BWI76_RS04865at 1016.545 kb on - strand, within BWI76_RS04865at 1016.612 kb on + strand, within BWI76_RS04865at 1016.617 kb on + strand, within BWI76_RS04865at 1016.632 kb on - strand, within BWI76_RS04865at 1016.632 kb on - strand, within BWI76_RS04865at 1016.754 kb on + strand, within BWI76_RS04865at 1016.755 kb on + strand, within BWI76_RS04865at 1016.757 kb on + strand, within BWI76_RS04865at 1016.986 kb on - strandat 1017.187 kb on - strandat 1017.428 kb on + strand, within BWI76_RS04870at 1017.429 kb on - strand, within BWI76_RS04870at 1017.524 kb on + strand, within BWI76_RS04870at 1017.524 kb on + strand, within BWI76_RS04870at 1017.532 kb on - strand, within BWI76_RS04870at 1017.560 kb on - strand, within BWI76_RS04870at 1017.588 kb on + strand, within BWI76_RS04870at 1017.808 kb on - strand, within BWI76_RS04870at 1017.808 kb on - strand, within BWI76_RS04870at 1017.837 kb on + strand, within BWI76_RS04870at 1017.837 kb on + strand, within BWI76_RS04870

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Per-strain Table

Position Strand Gene LocusTag Fraction rhizosphere EcoFAB sample 3; outgrowth in LB
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1,014,681 + BWI76_RS04860 0.14 +1.9
1,014,735 + BWI76_RS04860 0.19 +0.1
1,014,748 - BWI76_RS04860 0.21 -2.7
1,014,824 - BWI76_RS04860 0.29 +0.5
1,014,841 - BWI76_RS04860 0.30 +0.2
1,015,293 - BWI76_RS04860 0.78 -0.0
1,015,293 - BWI76_RS04860 0.78 -2.5
1,015,366 + BWI76_RS04860 0.86 -0.5
1,015,374 - BWI76_RS04860 0.87 -0.4
1,015,400 + BWI76_RS04860 0.89 +2.9
1,015,493 + +1.5
1,015,562 - +2.5
1,015,683 + BWI76_RS04865 0.13 -0.4
1,015,683 - BWI76_RS04865 0.13 +0.2
1,015,885 + BWI76_RS04865 0.27 -1.3
1,015,893 - +0.6
1,015,893 - BWI76_RS04865 0.28 +0.1
1,015,893 - BWI76_RS04865 0.28 +1.0
1,015,893 - BWI76_RS04865 0.28 -0.6
1,016,033 - BWI76_RS04865 0.38 -2.0
1,016,093 - BWI76_RS04865 0.42 -0.5
1,016,093 - BWI76_RS04865 0.42 +0.2
1,016,132 + BWI76_RS04865 0.45 +0.8
1,016,149 - BWI76_RS04865 0.46 +0.7
1,016,274 - -0.1
1,016,367 - BWI76_RS04865 0.62 +0.2
1,016,380 - BWI76_RS04865 0.62 -0.2
1,016,535 + BWI76_RS04865 0.73 -0.2
1,016,545 - BWI76_RS04865 0.74 +0.3
1,016,612 + BWI76_RS04865 0.79 +0.6
1,016,617 + BWI76_RS04865 0.79 +0.4
1,016,632 - BWI76_RS04865 0.80 +0.5
1,016,632 - BWI76_RS04865 0.80 -0.8
1,016,754 + BWI76_RS04865 0.89 -1.4
1,016,755 + BWI76_RS04865 0.89 -0.3
1,016,757 + BWI76_RS04865 0.89 +1.9
1,016,986 - -1.3
1,017,187 - -0.4
1,017,428 + BWI76_RS04870 0.25 +0.0
1,017,429 - BWI76_RS04870 0.25 -2.2
1,017,524 + BWI76_RS04870 0.32 -0.9
1,017,524 + BWI76_RS04870 0.32 +0.1
1,017,532 - BWI76_RS04870 0.33 -0.5
1,017,560 - BWI76_RS04870 0.35 +0.3
1,017,588 + BWI76_RS04870 0.37 -1.7
1,017,808 - BWI76_RS04870 0.53 -1.2
1,017,808 - BWI76_RS04870 0.53 -1.6
1,017,837 + BWI76_RS04870 0.55 -0.8
1,017,837 + BWI76_RS04870 0.55 -0.1

Or see this region's nucleotide sequence