Experiment: rhizosphere EcoFAB sample 3; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04575 and BWI76_RS04580 are separated by 214 nucleotides BWI76_RS04580 and BWI76_RS04585 are separated by 73 nucleotides BWI76_RS04585 and BWI76_RS04590 are separated by 10 nucleotides
BWI76_RS04575: BWI76_RS04575 - DNA polymerase II, at 949,877 to 952,234
_RS04575
BWI76_RS04580: BWI76_RS04580 - L-ribulose-5-phosphate 4-epimerase, at 952,449 to 953,144
_RS04580
BWI76_RS04585: BWI76_RS04585 - L-arabinose isomerase, at 953,218 to 954,720
_RS04585
BWI76_RS04590: BWI76_RS04590 - ribulokinase, at 954,731 to 956,440
_RS04590
Position (kb) 953
954
955 Strain fitness (log2 ratio) -2
-1
0
1
2 at 952.274 kb on - strand at 952.441 kb on - strand at 952.643 kb on + strand, within BWI76_RS04580 at 952.865 kb on - strand, within BWI76_RS04580 at 952.865 kb on - strand, within BWI76_RS04580 at 952.915 kb on - strand, within BWI76_RS04580 at 952.935 kb on - strand, within BWI76_RS04580 at 952.947 kb on - strand, within BWI76_RS04580 at 953.039 kb on + strand, within BWI76_RS04580 at 953.089 kb on + strand at 953.270 kb on - strand at 953.348 kb on - strand at 953.352 kb on + strand at 953.360 kb on - strand at 953.367 kb on - strand at 953.377 kb on - strand, within BWI76_RS04585 at 953.393 kb on + strand, within BWI76_RS04585 at 953.555 kb on - strand, within BWI76_RS04585 at 953.557 kb on + strand, within BWI76_RS04585 at 953.558 kb on + strand, within BWI76_RS04585 at 953.652 kb on - strand, within BWI76_RS04585 at 953.722 kb on - strand, within BWI76_RS04585 at 953.743 kb on + strand, within BWI76_RS04585 at 953.916 kb on + strand, within BWI76_RS04585 at 953.924 kb on - strand, within BWI76_RS04585 at 953.965 kb on - strand, within BWI76_RS04585 at 954.053 kb on + strand, within BWI76_RS04585 at 954.090 kb on + strand, within BWI76_RS04585 at 954.092 kb on + strand, within BWI76_RS04585 at 954.097 kb on + strand, within BWI76_RS04585 at 954.267 kb on + strand, within BWI76_RS04585 at 954.275 kb on - strand, within BWI76_RS04585 at 954.641 kb on + strand at 954.662 kb on - strand at 954.972 kb on + strand, within BWI76_RS04590 at 954.977 kb on + strand, within BWI76_RS04590 at 954.982 kb on + strand, within BWI76_RS04590 at 955.036 kb on - strand, within BWI76_RS04590 at 955.114 kb on + strand, within BWI76_RS04590 at 955.187 kb on + strand, within BWI76_RS04590 at 955.307 kb on - strand, within BWI76_RS04590 at 955.335 kb on + strand, within BWI76_RS04590 at 955.335 kb on + strand, within BWI76_RS04590 at 955.372 kb on - strand, within BWI76_RS04590 at 955.512 kb on - strand, within BWI76_RS04590 at 955.666 kb on - strand, within BWI76_RS04590
Per-strain Table
Position Strand Gene LocusTag Fraction rhizosphere EcoFAB sample 3; outgrowth in LB remove 952,274 - -1.0 952,441 - -0.8 952,643 + BWI76_RS04580 0.28 +0.9 952,865 - BWI76_RS04580 0.60 +1.9 952,865 - BWI76_RS04580 0.60 +0.7 952,915 - BWI76_RS04580 0.67 +1.5 952,935 - BWI76_RS04580 0.70 -0.0 952,947 - BWI76_RS04580 0.72 +0.3 953,039 + BWI76_RS04580 0.85 +0.4 953,089 + +0.8 953,270 - +0.8 953,348 - -0.2 953,352 + -0.3 953,360 - -0.6 953,367 - -0.4 953,377 - BWI76_RS04585 0.11 +0.9 953,393 + BWI76_RS04585 0.12 +0.6 953,555 - BWI76_RS04585 0.22 -2.0 953,557 + BWI76_RS04585 0.23 -1.1 953,558 + BWI76_RS04585 0.23 -0.3 953,652 - BWI76_RS04585 0.29 -0.4 953,722 - BWI76_RS04585 0.34 -0.7 953,743 + BWI76_RS04585 0.35 -0.6 953,916 + BWI76_RS04585 0.46 -0.1 953,924 - BWI76_RS04585 0.47 -0.1 953,965 - BWI76_RS04585 0.50 +0.4 954,053 + BWI76_RS04585 0.56 +0.3 954,090 + BWI76_RS04585 0.58 -0.3 954,092 + BWI76_RS04585 0.58 -0.5 954,097 + BWI76_RS04585 0.58 -0.4 954,267 + BWI76_RS04585 0.70 -0.1 954,275 - BWI76_RS04585 0.70 -0.7 954,641 + -1.9 954,662 - +0.6 954,972 + BWI76_RS04590 0.14 -1.3 954,977 + BWI76_RS04590 0.14 +1.5 954,982 + BWI76_RS04590 0.15 -0.1 955,036 - BWI76_RS04590 0.18 +0.2 955,114 + BWI76_RS04590 0.22 +1.1 955,187 + BWI76_RS04590 0.27 +0.0 955,307 - BWI76_RS04590 0.34 +0.4 955,335 + BWI76_RS04590 0.35 +0.5 955,335 + BWI76_RS04590 0.35 +0.4 955,372 - BWI76_RS04590 0.37 +1.9 955,512 - BWI76_RS04590 0.46 +0.4 955,666 - BWI76_RS04590 0.55 +0.3
Or see this region's nucleotide sequence