Experiment: rhizosphere EcoFAB sample 3; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02580 and BWI76_RS02585 are separated by 45 nucleotides BWI76_RS02585 and BWI76_RS02590 are separated by 105 nucleotides BWI76_RS02590 and BWI76_RS02595 overlap by 10 nucleotides BWI76_RS02595 and BWI76_RS02600 are separated by 138 nucleotides BWI76_RS02600 and BWI76_RS02605 are separated by 176 nucleotides
BWI76_RS02580: BWI76_RS02580 - 23S rRNA (guanosine(2251)-2'-O)-methyltransferase, at 529,269 to 530,000
_RS02580
BWI76_RS02585: BWI76_RS02585 - hypothetical protein, at 530,046 to 530,399
_RS02585
BWI76_RS02590: BWI76_RS02590 - isovaleryl-CoA dehydrogenase, at 530,505 to 532,142
_RS02590
BWI76_RS02595: BWI76_RS02595 - hypothetical protein, at 532,133 to 532,408
_RS02595
BWI76_RS02600: BWI76_RS02600 - biofilm peroxide resistance protein BsmA, at 532,547 to 532,876
_RS02600
BWI76_RS02605: BWI76_RS02605 - esterase, at 533,053 to 533,769
_RS02605
Position (kb) 530
531
532
533 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 529.667 kb on + strand, within BWI76_RS02580 at 529.792 kb on + strand, within BWI76_RS02580 at 529.804 kb on + strand, within BWI76_RS02580 at 529.971 kb on - strand at 530.042 kb on - strand at 530.042 kb on - strand at 530.114 kb on - strand, within BWI76_RS02585 at 530.114 kb on - strand, within BWI76_RS02585 at 530.253 kb on + strand, within BWI76_RS02585 at 530.286 kb on - strand, within BWI76_RS02585 at 530.351 kb on + strand, within BWI76_RS02585 at 530.353 kb on + strand, within BWI76_RS02585 at 530.526 kb on - strand at 530.552 kb on - strand at 530.827 kb on + strand, within BWI76_RS02590 at 530.931 kb on - strand, within BWI76_RS02590 at 531.256 kb on - strand, within BWI76_RS02590 at 531.261 kb on + strand, within BWI76_RS02590 at 531.264 kb on - strand, within BWI76_RS02590 at 531.351 kb on + strand, within BWI76_RS02590 at 531.419 kb on - strand, within BWI76_RS02590 at 531.421 kb on + strand, within BWI76_RS02590 at 531.448 kb on - strand, within BWI76_RS02590 at 531.458 kb on + strand, within BWI76_RS02590 at 531.522 kb on + strand, within BWI76_RS02590 at 531.598 kb on - strand, within BWI76_RS02590 at 531.709 kb on + strand, within BWI76_RS02590 at 531.737 kb on + strand, within BWI76_RS02590 at 531.942 kb on - strand, within BWI76_RS02590 at 531.996 kb on + strand at 532.143 kb on + strand at 532.195 kb on - strand, within BWI76_RS02595 at 532.244 kb on - strand, within BWI76_RS02595 at 532.246 kb on + strand, within BWI76_RS02595 at 532.370 kb on + strand, within BWI76_RS02595 at 532.437 kb on - strand at 532.437 kb on - strand at 532.437 kb on - strand at 532.538 kb on - strand at 532.596 kb on - strand, within BWI76_RS02600 at 532.644 kb on + strand, within BWI76_RS02600 at 532.652 kb on - strand, within BWI76_RS02600 at 532.690 kb on - strand, within BWI76_RS02600 at 532.757 kb on + strand, within BWI76_RS02600 at 532.789 kb on + strand, within BWI76_RS02600 at 532.795 kb on - strand, within BWI76_RS02600 at 532.834 kb on + strand, within BWI76_RS02600 at 532.834 kb on + strand, within BWI76_RS02600 at 532.834 kb on + strand, within BWI76_RS02600 at 532.842 kb on - strand, within BWI76_RS02600 at 532.842 kb on - strand, within BWI76_RS02600 at 532.842 kb on - strand, within BWI76_RS02600 at 532.878 kb on + strand at 532.886 kb on - strand at 533.068 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction rhizosphere EcoFAB sample 3; outgrowth in LB remove 529,667 + BWI76_RS02580 0.54 -1.1 529,792 + BWI76_RS02580 0.71 -1.7 529,804 + BWI76_RS02580 0.73 -1.4 529,971 - -1.1 530,042 - -0.6 530,042 - -0.4 530,114 - BWI76_RS02585 0.19 -0.4 530,114 - BWI76_RS02585 0.19 -0.1 530,253 + BWI76_RS02585 0.58 -0.1 530,286 - BWI76_RS02585 0.68 +1.3 530,351 + BWI76_RS02585 0.86 +0.3 530,353 + BWI76_RS02585 0.87 +0.1 530,526 - +1.4 530,552 - -0.0 530,827 + BWI76_RS02590 0.20 -2.0 530,931 - BWI76_RS02590 0.26 -2.7 531,256 - BWI76_RS02590 0.46 -0.6 531,261 + BWI76_RS02590 0.46 -0.8 531,264 - BWI76_RS02590 0.46 -0.5 531,351 + BWI76_RS02590 0.52 -2.6 531,419 - BWI76_RS02590 0.56 -3.5 531,421 + BWI76_RS02590 0.56 -2.3 531,448 - BWI76_RS02590 0.58 -0.3 531,458 + BWI76_RS02590 0.58 -0.1 531,522 + BWI76_RS02590 0.62 -2.3 531,598 - BWI76_RS02590 0.67 -0.4 531,709 + BWI76_RS02590 0.74 -0.6 531,737 + BWI76_RS02590 0.75 -3.3 531,942 - BWI76_RS02590 0.88 -0.4 531,996 + -2.8 532,143 + -0.2 532,195 - BWI76_RS02595 0.22 -0.4 532,244 - BWI76_RS02595 0.40 +0.7 532,246 + BWI76_RS02595 0.41 +1.4 532,370 + BWI76_RS02595 0.86 +1.8 532,437 - +1.7 532,437 - +1.2 532,437 - +1.7 532,538 - +1.7 532,596 - BWI76_RS02600 0.15 +1.3 532,644 + BWI76_RS02600 0.29 +1.9 532,652 - BWI76_RS02600 0.32 +1.3 532,690 - BWI76_RS02600 0.43 +2.1 532,757 + BWI76_RS02600 0.64 +1.7 532,789 + BWI76_RS02600 0.73 +2.0 532,795 - BWI76_RS02600 0.75 +1.3 532,834 + BWI76_RS02600 0.87 +2.1 532,834 + BWI76_RS02600 0.87 +1.7 532,834 + BWI76_RS02600 0.87 +0.9 532,842 - BWI76_RS02600 0.89 +1.6 532,842 - BWI76_RS02600 0.89 +1.6 532,842 - BWI76_RS02600 0.89 +1.7 532,878 + +1.1 532,886 - +1.1 533,068 - +0.3
Or see this region's nucleotide sequence