Experiment: rhizosphere EcoFAB sample 3; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02430 and BWI76_RS02435 overlap by 4 nucleotides BWI76_RS02435 and BWI76_RS02440 are separated by 140 nucleotides BWI76_RS02440 and BWI76_RS02445 are separated by 10 nucleotides BWI76_RS02445 and BWI76_RS02450 are separated by 10 nucleotides
BWI76_RS02430: BWI76_RS02430 - multidrug transporter, at 499,179 to 499,496
_RS02430
BWI76_RS02435: BWI76_RS02435 - outer membrane lipoprotein Blc, at 499,493 to 500,023
_RS02435
BWI76_RS02440: BWI76_RS02440 - fumarate reductase subunit D, at 500,164 to 500,523
_RS02440
BWI76_RS02445: BWI76_RS02445 - fumarate reductase subunit C, at 500,534 to 500,929
_RS02445
BWI76_RS02450: BWI76_RS02450 - succinate dehydrogenase/fumarate reductase iron-sulfur subunit, at 500,940 to 501,674
_RS02450
Position (kb)
500
501 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 499.196 kb on + strand at 499.202 kb on - strand at 499.211 kb on + strand, within BWI76_RS02430 at 499.211 kb on + strand, within BWI76_RS02430 at 499.219 kb on - strand, within BWI76_RS02430 at 499.220 kb on + strand, within BWI76_RS02430 at 499.220 kb on + strand, within BWI76_RS02430 at 499.308 kb on + strand, within BWI76_RS02430 at 499.324 kb on - strand, within BWI76_RS02430 at 499.359 kb on + strand, within BWI76_RS02430 at 499.392 kb on - strand, within BWI76_RS02430 at 499.397 kb on - strand, within BWI76_RS02430 at 499.468 kb on + strand at 499.491 kb on + strand at 499.499 kb on - strand at 499.510 kb on + strand at 499.606 kb on + strand, within BWI76_RS02435 at 499.614 kb on - strand, within BWI76_RS02435 at 499.828 kb on + strand, within BWI76_RS02435 at 499.836 kb on - strand, within BWI76_RS02435 at 499.836 kb on - strand, within BWI76_RS02435 at 499.836 kb on - strand, within BWI76_RS02435 at 499.934 kb on - strand, within BWI76_RS02435 at 499.960 kb on + strand, within BWI76_RS02435 at 499.960 kb on + strand, within BWI76_RS02435 at 499.960 kb on + strand, within BWI76_RS02435 at 500.052 kb on + strand at 500.299 kb on - strand, within BWI76_RS02440 at 500.304 kb on + strand, within BWI76_RS02440 at 500.463 kb on - strand, within BWI76_RS02440 at 500.543 kb on - strand at 500.543 kb on - strand at 500.586 kb on + strand, within BWI76_RS02445 at 500.762 kb on + strand, within BWI76_RS02445 at 500.848 kb on + strand, within BWI76_RS02445 at 500.850 kb on + strand, within BWI76_RS02445 at 500.911 kb on - strand at 500.948 kb on + strand at 500.956 kb on - strand at 500.994 kb on - strand at 501.287 kb on + strand, within BWI76_RS02450 at 501.420 kb on - strand, within BWI76_RS02450 at 501.475 kb on - strand, within BWI76_RS02450 at 501.505 kb on + strand, within BWI76_RS02450
Per-strain Table
Position Strand Gene LocusTag Fraction rhizosphere EcoFAB sample 3; outgrowth in LB remove 499,196 + +0.3 499,202 - -0.1 499,211 + BWI76_RS02430 0.10 +0.3 499,211 + BWI76_RS02430 0.10 +0.2 499,219 - BWI76_RS02430 0.13 -0.1 499,220 + BWI76_RS02430 0.13 +0.8 499,220 + BWI76_RS02430 0.13 +1.5 499,308 + BWI76_RS02430 0.41 -2.3 499,324 - BWI76_RS02430 0.46 +0.5 499,359 + BWI76_RS02430 0.57 +1.1 499,392 - BWI76_RS02430 0.67 +1.2 499,397 - BWI76_RS02430 0.69 +0.2 499,468 + -1.7 499,491 + +0.6 499,499 - +0.2 499,510 + -1.4 499,606 + BWI76_RS02435 0.21 -1.7 499,614 - BWI76_RS02435 0.23 +0.7 499,828 + BWI76_RS02435 0.63 +0.8 499,836 - BWI76_RS02435 0.65 +0.7 499,836 - BWI76_RS02435 0.65 +0.4 499,836 - BWI76_RS02435 0.65 -0.1 499,934 - BWI76_RS02435 0.83 +1.1 499,960 + BWI76_RS02435 0.88 +1.1 499,960 + BWI76_RS02435 0.88 +0.7 499,960 + BWI76_RS02435 0.88 +0.4 500,052 + -1.2 500,299 - BWI76_RS02440 0.38 -1.3 500,304 + BWI76_RS02440 0.39 -0.3 500,463 - BWI76_RS02440 0.83 -0.5 500,543 - -0.9 500,543 - -0.7 500,586 + BWI76_RS02445 0.13 +1.1 500,762 + BWI76_RS02445 0.58 -3.7 500,848 + BWI76_RS02445 0.79 -1.2 500,850 + BWI76_RS02445 0.80 -0.0 500,911 - -3.5 500,948 + -1.6 500,956 - +0.5 500,994 - +0.1 501,287 + BWI76_RS02450 0.47 -0.2 501,420 - BWI76_RS02450 0.65 -0.5 501,475 - BWI76_RS02450 0.73 -1.3 501,505 + BWI76_RS02450 0.77 -2.9
Or see this region's nucleotide sequence