Experiment: rhizosphere EcoFAB sample 3; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01240 and BWI76_RS01245 are separated by 67 nucleotides BWI76_RS01245 and BWI76_RS01250 are separated by 61 nucleotides BWI76_RS01250 and BWI76_RS01255 are separated by 246 nucleotides
BWI76_RS01240: BWI76_RS01240 - phospholipase A, at 253,655 to 254,527
_RS01240
BWI76_RS01245: BWI76_RS01245 - DNA helicase RecQ, at 254,595 to 256,421
_RS01245
BWI76_RS01250: BWI76_RS01250 - threonine export protein RhtC, at 256,483 to 257,106
_RS01250
BWI76_RS01255: BWI76_RS01255 - homoserine/homoserine lactone efflux protein, at 257,353 to 257,973
_RS01255
Position (kb) 254
255
256
257 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 253.615 kb on - strand at 253.657 kb on + strand at 253.664 kb on + strand at 253.664 kb on + strand at 253.672 kb on - strand at 253.672 kb on - strand at 253.743 kb on + strand, within BWI76_RS01240 at 253.815 kb on + strand, within BWI76_RS01240 at 253.823 kb on - strand, within BWI76_RS01240 at 253.939 kb on - strand, within BWI76_RS01240 at 254.025 kb on - strand, within BWI76_RS01240 at 254.123 kb on + strand, within BWI76_RS01240 at 254.316 kb on + strand, within BWI76_RS01240 at 254.331 kb on + strand, within BWI76_RS01240 at 254.339 kb on - strand, within BWI76_RS01240 at 254.427 kb on + strand, within BWI76_RS01240 at 254.437 kb on + strand, within BWI76_RS01240 at 254.639 kb on + strand at 254.703 kb on + strand at 254.722 kb on + strand at 254.722 kb on + strand at 254.769 kb on - strand at 254.831 kb on + strand, within BWI76_RS01245 at 254.901 kb on - strand, within BWI76_RS01245 at 255.222 kb on - strand, within BWI76_RS01245 at 255.654 kb on - strand, within BWI76_RS01245 at 255.695 kb on + strand, within BWI76_RS01245 at 255.739 kb on + strand, within BWI76_RS01245 at 255.885 kb on + strand, within BWI76_RS01245 at 255.885 kb on + strand, within BWI76_RS01245 at 255.885 kb on + strand, within BWI76_RS01245 at 255.963 kb on + strand, within BWI76_RS01245 at 256.006 kb on + strand, within BWI76_RS01245 at 256.258 kb on + strand at 256.357 kb on - strand at 256.376 kb on - strand at 256.408 kb on + strand at 256.535 kb on + strand at 256.543 kb on - strand at 256.633 kb on - strand, within BWI76_RS01250 at 256.643 kb on + strand, within BWI76_RS01250 at 256.643 kb on + strand, within BWI76_RS01250 at 256.651 kb on - strand, within BWI76_RS01250 at 256.656 kb on - strand, within BWI76_RS01250 at 256.718 kb on + strand, within BWI76_RS01250 at 256.807 kb on - strand, within BWI76_RS01250 at 256.875 kb on - strand, within BWI76_RS01250 at 257.129 kb on + strand at 257.137 kb on - strand at 257.179 kb on + strand at 257.379 kb on - strand at 257.379 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction rhizosphere EcoFAB sample 3; outgrowth in LB remove 253,615 - -0.2 253,657 + +0.7 253,664 + -0.1 253,664 + +2.2 253,672 - +0.8 253,672 - -0.7 253,743 + BWI76_RS01240 0.10 +0.6 253,815 + BWI76_RS01240 0.18 +1.0 253,823 - BWI76_RS01240 0.19 -1.1 253,939 - BWI76_RS01240 0.33 +0.1 254,025 - BWI76_RS01240 0.42 -3.0 254,123 + BWI76_RS01240 0.54 +2.1 254,316 + BWI76_RS01240 0.76 +0.3 254,331 + BWI76_RS01240 0.77 +0.7 254,339 - BWI76_RS01240 0.78 +1.3 254,427 + BWI76_RS01240 0.88 -1.2 254,437 + BWI76_RS01240 0.90 +1.6 254,639 + +0.6 254,703 + -0.8 254,722 + -1.6 254,722 + -2.7 254,769 - +0.6 254,831 + BWI76_RS01245 0.13 -0.2 254,901 - BWI76_RS01245 0.17 +0.4 255,222 - BWI76_RS01245 0.34 +0.4 255,654 - BWI76_RS01245 0.58 -2.4 255,695 + BWI76_RS01245 0.60 -1.7 255,739 + BWI76_RS01245 0.63 -0.5 255,885 + BWI76_RS01245 0.71 +2.5 255,885 + BWI76_RS01245 0.71 -2.0 255,885 + BWI76_RS01245 0.71 -0.0 255,963 + BWI76_RS01245 0.75 +1.3 256,006 + BWI76_RS01245 0.77 +1.1 256,258 + +0.4 256,357 - +0.8 256,376 - +0.7 256,408 + +1.2 256,535 + -0.1 256,543 - +0.0 256,633 - BWI76_RS01250 0.24 +0.1 256,643 + BWI76_RS01250 0.26 -0.2 256,643 + BWI76_RS01250 0.26 +1.0 256,651 - BWI76_RS01250 0.27 -2.2 256,656 - BWI76_RS01250 0.28 -2.6 256,718 + BWI76_RS01250 0.38 +0.2 256,807 - BWI76_RS01250 0.52 -0.5 256,875 - BWI76_RS01250 0.63 -0.6 257,129 + -1.4 257,137 - +0.4 257,179 + +3.0 257,379 - -0.9 257,379 - -0.3
Or see this region's nucleotide sequence