Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00695

Experiment: rhizosphere EcoFAB sample 3; outgrowth in LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00685 and BWI76_RS00690 are separated by 50 nucleotidesBWI76_RS00690 and BWI76_RS00695 are separated by 41 nucleotidesBWI76_RS00695 and BWI76_RS00700 are separated by 5 nucleotides BWI76_RS00685: BWI76_RS00685 - ABC transporter ATP-binding protein, at 140,828 to 140,929 _RS00685 BWI76_RS00690: BWI76_RS00690 - transcriptional activator RhaS, at 140,980 to 141,816 _RS00690 BWI76_RS00695: BWI76_RS00695 - AraC family transcriptional regulator, at 141,858 to 142,739 _RS00695 BWI76_RS00700: BWI76_RS00700 - rhamnose/proton symporter RhaT, at 142,745 to 143,779 _RS00700 Position (kb) 141 142 143Strain fitness (log2 ratio) -2 -1 0 1at 140.985 kb on - strandat 140.985 kb on - strandat 140.986 kb on + strandat 140.986 kb on + strandat 140.986 kb on + strandat 140.986 kb on + strandat 141.138 kb on + strand, within BWI76_RS00690at 141.138 kb on + strand, within BWI76_RS00690at 141.138 kb on + strand, within BWI76_RS00690at 141.226 kb on + strand, within BWI76_RS00690at 141.328 kb on + strand, within BWI76_RS00690at 141.368 kb on + strand, within BWI76_RS00690at 141.369 kb on + strandat 141.825 kb on - strandat 142.002 kb on - strand, within BWI76_RS00695at 142.044 kb on - strand, within BWI76_RS00695at 142.045 kb on + strand, within BWI76_RS00695at 142.149 kb on + strand, within BWI76_RS00695at 142.211 kb on + strand, within BWI76_RS00695at 142.263 kb on - strand, within BWI76_RS00695at 142.309 kb on + strand, within BWI76_RS00695at 142.327 kb on - strand, within BWI76_RS00695at 142.327 kb on - strand, within BWI76_RS00695at 142.411 kb on + strand, within BWI76_RS00695at 142.461 kb on - strand, within BWI76_RS00695at 142.601 kb on + strand, within BWI76_RS00695at 142.604 kb on - strand, within BWI76_RS00695at 142.645 kb on + strand, within BWI76_RS00695at 142.653 kb on - strandat 142.908 kb on - strand, within BWI76_RS00700at 142.952 kb on - strand, within BWI76_RS00700at 143.140 kb on - strand, within BWI76_RS00700at 143.145 kb on + strand, within BWI76_RS00700at 143.153 kb on - strand, within BWI76_RS00700at 143.155 kb on + strand, within BWI76_RS00700at 143.163 kb on - strand, within BWI76_RS00700at 143.163 kb on - strand, within BWI76_RS00700at 143.243 kb on - strand, within BWI76_RS00700at 143.255 kb on - strand, within BWI76_RS00700at 143.305 kb on + strand, within BWI76_RS00700at 143.313 kb on - strand, within BWI76_RS00700at 143.324 kb on + strand, within BWI76_RS00700at 143.332 kb on - strand, within BWI76_RS00700at 143.390 kb on - strand, within BWI76_RS00700at 143.616 kb on - strand, within BWI76_RS00700at 143.673 kb on - strand, within BWI76_RS00700at 143.714 kb on + strandat 143.724 kb on + strandat 143.724 kb on + strandat 143.732 kb on - strandat 143.732 kb on - strandat 143.732 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction rhizosphere EcoFAB sample 3; outgrowth in LB
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140,985 - +0.6
140,985 - +1.3
140,986 + +0.9
140,986 + -1.1
140,986 + +0.5
140,986 + +0.0
141,138 + BWI76_RS00690 0.19 -0.6
141,138 + BWI76_RS00690 0.19 -0.3
141,138 + BWI76_RS00690 0.19 +0.5
141,226 + BWI76_RS00690 0.29 +0.4
141,328 + BWI76_RS00690 0.42 +0.9
141,368 + BWI76_RS00690 0.46 +0.7
141,369 + -1.0
141,825 - +1.2
142,002 - BWI76_RS00695 0.16 -0.1
142,044 - BWI76_RS00695 0.21 +1.1
142,045 + BWI76_RS00695 0.21 +0.4
142,149 + BWI76_RS00695 0.33 +0.4
142,211 + BWI76_RS00695 0.40 +0.7
142,263 - BWI76_RS00695 0.46 -2.4
142,309 + BWI76_RS00695 0.51 +0.6
142,327 - BWI76_RS00695 0.53 -0.2
142,327 - BWI76_RS00695 0.53 -0.6
142,411 + BWI76_RS00695 0.63 +1.3
142,461 - BWI76_RS00695 0.68 +0.9
142,601 + BWI76_RS00695 0.84 +0.1
142,604 - BWI76_RS00695 0.85 +1.4
142,645 + BWI76_RS00695 0.89 -0.2
142,653 - +1.2
142,908 - BWI76_RS00700 0.16 -0.7
142,952 - BWI76_RS00700 0.20 +0.3
143,140 - BWI76_RS00700 0.38 +0.3
143,145 + BWI76_RS00700 0.39 +0.3
143,153 - BWI76_RS00700 0.39 -2.2
143,155 + BWI76_RS00700 0.40 -1.2
143,163 - BWI76_RS00700 0.40 -0.7
143,163 - BWI76_RS00700 0.40 +1.1
143,243 - BWI76_RS00700 0.48 +0.4
143,255 - BWI76_RS00700 0.49 +0.3
143,305 + BWI76_RS00700 0.54 -0.0
143,313 - BWI76_RS00700 0.55 -1.3
143,324 + BWI76_RS00700 0.56 -1.7
143,332 - BWI76_RS00700 0.57 +0.2
143,390 - BWI76_RS00700 0.62 -0.2
143,616 - BWI76_RS00700 0.84 +0.1
143,673 - BWI76_RS00700 0.90 +0.4
143,714 + -0.8
143,724 + +0.6
143,724 + +0.1
143,732 - +1.0
143,732 - +0.5
143,732 - -1.4

Or see this region's nucleotide sequence