Experiment: rhizosphere EcoFAB sample 3; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00290 and BWI76_RS00295 are separated by 12 nucleotides BWI76_RS00295 and BWI76_RS00300 overlap by 4 nucleotides
BWI76_RS00290: BWI76_RS00290 - Ribokinase (EC 2.7.1.15) (from data) , at 60,235 to 61,164
_RS00290
BWI76_RS00295: BWI76_RS00295 - transcriptional regulator RbsR, at 61,177 to 62,160
_RS00295
BWI76_RS00300: BWI76_RS00300 - MFS transporter, at 62,157 to 63,554
_RS00300
Position (kb)
61
62
63 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 60.261 kb on - strand at 60.289 kb on + strand at 60.371 kb on + strand, within BWI76_RS00290 at 60.462 kb on + strand, within BWI76_RS00290 at 60.500 kb on - strand, within BWI76_RS00290 at 60.505 kb on - strand, within BWI76_RS00290 at 60.519 kb on - strand, within BWI76_RS00290 at 60.597 kb on - strand at 60.599 kb on - strand, within BWI76_RS00290 at 60.635 kb on + strand, within BWI76_RS00290 at 60.656 kb on + strand, within BWI76_RS00290 at 60.734 kb on - strand, within BWI76_RS00290 at 60.734 kb on - strand, within BWI76_RS00290 at 60.793 kb on + strand, within BWI76_RS00290 at 60.820 kb on - strand, within BWI76_RS00290 at 60.821 kb on - strand, within BWI76_RS00290 at 60.865 kb on - strand, within BWI76_RS00290 at 61.113 kb on - strand at 61.165 kb on + strand at 61.173 kb on - strand at 61.431 kb on + strand, within BWI76_RS00295 at 61.433 kb on + strand, within BWI76_RS00295 at 61.439 kb on + strand, within BWI76_RS00295 at 61.441 kb on - strand, within BWI76_RS00295 at 61.441 kb on - strand, within BWI76_RS00295 at 61.447 kb on - strand, within BWI76_RS00295 at 61.457 kb on - strand, within BWI76_RS00295 at 61.580 kb on - strand, within BWI76_RS00295 at 61.960 kb on + strand, within BWI76_RS00295 at 61.960 kb on + strand, within BWI76_RS00295 at 61.968 kb on - strand, within BWI76_RS00295 at 62.007 kb on + strand, within BWI76_RS00295 at 62.102 kb on + strand at 62.115 kb on - strand at 62.137 kb on - strand at 62.143 kb on - strand at 62.163 kb on - strand at 62.169 kb on - strand at 62.273 kb on + strand at 62.429 kb on + strand, within BWI76_RS00300 at 62.470 kb on + strand, within BWI76_RS00300 at 62.584 kb on - strand, within BWI76_RS00300 at 62.599 kb on - strand, within BWI76_RS00300 at 62.633 kb on + strand, within BWI76_RS00300 at 62.673 kb on + strand, within BWI76_RS00300 at 62.730 kb on - strand, within BWI76_RS00300 at 62.814 kb on - strand, within BWI76_RS00300 at 63.029 kb on - strand, within BWI76_RS00300 at 63.075 kb on + strand, within BWI76_RS00300 at 63.088 kb on - strand, within BWI76_RS00300 at 63.098 kb on - strand, within BWI76_RS00300 at 63.110 kb on - strand, within BWI76_RS00300 at 63.115 kb on - strand, within BWI76_RS00300 at 63.129 kb on - strand, within BWI76_RS00300 at 63.129 kb on - strand, within BWI76_RS00300 at 63.130 kb on - strand, within BWI76_RS00300
Per-strain Table
Position Strand Gene LocusTag Fraction rhizosphere EcoFAB sample 3; outgrowth in LB remove 60,261 - -2.8 60,289 + -2.4 60,371 + BWI76_RS00290 0.15 -1.4 60,462 + BWI76_RS00290 0.24 -0.7 60,500 - BWI76_RS00290 0.28 -0.7 60,505 - BWI76_RS00290 0.29 -4.1 60,519 - BWI76_RS00290 0.31 -1.2 60,597 - -1.0 60,599 - BWI76_RS00290 0.39 -0.3 60,635 + BWI76_RS00290 0.43 -2.1 60,656 + BWI76_RS00290 0.45 -1.0 60,734 - BWI76_RS00290 0.54 -4.8 60,734 - BWI76_RS00290 0.54 -1.9 60,793 + BWI76_RS00290 0.60 -1.7 60,820 - BWI76_RS00290 0.63 -3.4 60,821 - BWI76_RS00290 0.63 -1.6 60,865 - BWI76_RS00290 0.68 -4.1 61,113 - -5.6 61,165 + -4.5 61,173 - -4.1 61,431 + BWI76_RS00295 0.26 -2.0 61,433 + BWI76_RS00295 0.26 -3.9 61,439 + BWI76_RS00295 0.27 -4.5 61,441 - BWI76_RS00295 0.27 -0.9 61,441 - BWI76_RS00295 0.27 -5.0 61,447 - BWI76_RS00295 0.27 -3.5 61,457 - BWI76_RS00295 0.28 -1.7 61,580 - BWI76_RS00295 0.41 -2.6 61,960 + BWI76_RS00295 0.80 -4.7 61,960 + BWI76_RS00295 0.80 -2.6 61,968 - BWI76_RS00295 0.80 -3.2 62,007 + BWI76_RS00295 0.84 -0.5 62,102 + -2.0 62,115 - -3.8 62,137 - -5.0 62,143 - -3.1 62,163 - +0.8 62,169 - +0.4 62,273 + +0.6 62,429 + BWI76_RS00300 0.19 -0.6 62,470 + BWI76_RS00300 0.22 -0.3 62,584 - BWI76_RS00300 0.31 -0.1 62,599 - BWI76_RS00300 0.32 +0.4 62,633 + BWI76_RS00300 0.34 -2.1 62,673 + BWI76_RS00300 0.37 +0.2 62,730 - BWI76_RS00300 0.41 +1.2 62,814 - BWI76_RS00300 0.47 -0.5 63,029 - BWI76_RS00300 0.62 +1.6 63,075 + BWI76_RS00300 0.66 -0.2 63,088 - BWI76_RS00300 0.67 -0.5 63,098 - BWI76_RS00300 0.67 -0.1 63,110 - BWI76_RS00300 0.68 +0.8 63,115 - BWI76_RS00300 0.69 -0.6 63,129 - BWI76_RS00300 0.70 +0.5 63,129 - BWI76_RS00300 0.70 -0.4 63,130 - BWI76_RS00300 0.70 +1.2
Or see this region's nucleotide sequence