Experiment: H2 50 mM hypophosphite
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06490 and MMP_RS06495 are separated by 17 nucleotides MMP_RS06495 and MMP_RS06500 are separated by 15 nucleotides MMP_RS06500 and MMP_RS06505 are separated by 77 nucleotides
MMP_RS06490: MMP_RS06490 - hypothetical protein, at 1,245,458 to 1,245,985
_RS06490
MMP_RS06495: MMP_RS06495 - YkgJ family cysteine cluster protein, at 1,246,003 to 1,246,815
_RS06495
MMP_RS06500: MMP_RS06500 - ATP-binding protein, at 1,246,831 to 1,248,324
_RS06500
MMP_RS06505: MMP_RS06505 - Glu-tRNA(Gln) amidotransferase subunit GatE, at 1,248,402 to 1,250,297
_RS06505
Position (kb)
1246
1247
1248
1249 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1245.920 kb on - strand, within MMP_RS06490 at 1245.985 kb on - strand at 1245.985 kb on - strand at 1246.057 kb on + strand at 1246.087 kb on + strand, within MMP_RS06495 at 1246.087 kb on + strand, within MMP_RS06495 at 1246.138 kb on + strand, within MMP_RS06495 at 1246.761 kb on + strand at 1246.782 kb on + strand at 1247.095 kb on - strand, within MMP_RS06500 at 1247.273 kb on - strand, within MMP_RS06500 at 1247.498 kb on + strand, within MMP_RS06500 at 1247.712 kb on - strand, within MMP_RS06500 at 1247.713 kb on + strand, within MMP_RS06500 at 1247.726 kb on + strand, within MMP_RS06500 at 1247.742 kb on - strand, within MMP_RS06500 at 1247.831 kb on + strand, within MMP_RS06500 at 1247.894 kb on + strand, within MMP_RS06500 at 1247.917 kb on - strand, within MMP_RS06500 at 1248.300 kb on - strand at 1248.306 kb on + strand at 1248.348 kb on + strand at 1248.352 kb on - strand at 1248.356 kb on - strand at 1248.470 kb on - strand at 1248.553 kb on + strand at 1248.910 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 50 mM hypophosphite remove 1,245,920 - MMP_RS06490 0.88 +0.2 1,245,985 - +0.4 1,245,985 - +0.8 1,246,057 + -1.3 1,246,087 + MMP_RS06495 0.10 -1.6 1,246,087 + MMP_RS06495 0.10 -1.1 1,246,138 + MMP_RS06495 0.17 -0.9 1,246,761 + -0.1 1,246,782 + +0.3 1,247,095 - MMP_RS06500 0.18 -2.8 1,247,273 - MMP_RS06500 0.30 -0.3 1,247,498 + MMP_RS06500 0.45 -0.9 1,247,712 - MMP_RS06500 0.59 -2.2 1,247,713 + MMP_RS06500 0.59 -2.2 1,247,726 + MMP_RS06500 0.60 -2.8 1,247,742 - MMP_RS06500 0.61 -3.7 1,247,831 + MMP_RS06500 0.67 -1.7 1,247,894 + MMP_RS06500 0.71 -1.5 1,247,917 - MMP_RS06500 0.73 -2.2 1,248,300 - -0.5 1,248,306 + -1.3 1,248,348 + -0.9 1,248,352 - -1.4 1,248,356 - +0.7 1,248,470 - +2.0 1,248,553 + +1.9 1,248,910 + +0.3
Or see this region's nucleotide sequence